Telah dilakukan penelitian yang bertujuan mengetahui pengaruh fortifikan NaFeEDTA dalam susu kedelai terhadap kadar hemoglobin darah tikus Rattus norvegicus L. jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap RAL . Sebanyak 25 ekor tikus putih jantan dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar tanpa pemberian susu kedelai dan fortifikan; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA dosis 2,7 mgFe/ kgBB; 5,4 mgFe/ kg BB; dan 10,8 mgFe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Pengukuran kadar hemoglobin darah tikus menggunakan hematology analyzer. Hasil uji ANAVA satu arah dan uji LSD P < 0,05 menunjukkan bahwa setelah pemberian perlakuan selama 21 hari berturut-turut, terdapat perbedaan kadar hemoglobin yang nyata antara seluruh kelompok perlakuan KP 1, KP 2 dan KP 3 terhadap kadar hemoglobin KK 1 dan perbedaan kadar hemoglobin yang nyata antara KP 2 dan KP 3 terhadap kadar hemoglobin KK 2. Peningkatan kadar hemoglobin tertinggi terjadi pada KP 2, yaitu 10,84 terhadap KK 1; dan 9,28 terhadap KK 2.

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The effect of NaFeEDTA fortificant in soymilk on blood hemoglobin levels of male Sprague Dawley rats Rattus norvegicus L. had been studied. By using Complete Random Design CRD , twenty five rats were divided into five groups. Normal control group KK 1 was administered with standard feeding and drinking without adding soymilk and fortificant. Treatment control group KK 2 was administered with soymilk without fortificant, and three treatment groups which were administered with soymilk added with NaFeEDTA fortificant 2.7 mg Fe kgbw KP 1 5.4 mg Fe kgbw KP 2 and 10.8 mg Fe kgbw KP 3 . All of the five groups were treated for 21 days consecutively. Measurement of rat blood hemoglobin levels uses the hematology analyzer. One way ANOVA test and post hoc LSD test P 0.05 showed that after 21 days of consecutive treatment, there was a significant effect on blood hemoglobin levels in all treatment groups KP 1, KP 2, and KP 3 compared to KP 1 and a significant effect on blood hemoglobin levels in KP 2 and KP 3 compared to KK 2. The highest increase of blood hemoglobin levels was detected on KP 2 which is 10.84 to KK 1 and 9.28 to KK 2.