Latar Belakang: Pemeriksaan histopatologi pada apendisitis akut dianggap sebagaipemeriksaan baku emas, walaupun tidak selalu dapat membuktikan adanyaperadangan akut. Hal tersebut menimbulkan dugaan adanya patogenesis lain yangbelum diketahui. Beberapa penelitian menemukan adanya korelasi antara sel mastdengan saraf enterik pada apendisitis akut. Tujuan penelitian ini adalah melihatkepadatan sel mast dan jaringan saraf, serta korelasi derajat kepadatan sel mastdengan derajat kepadatan jaringan saraf pada dinding apendisitis akut. Bahan dancara kerja: Penelitian observasional analitik potong lintang dilakukan pada 97sediaan histopatologi apendisitis akut yang dikelompokkan menjadi apendisitis akutfokal, supuratif, gangrenosa dan perforatif. Penilaian sel mast menggunakan pulasanToluidine blue dan penilaian jaringan saraf menggunakan pulasan IHK S100.Kemudian dilakukan penilaian korelasi derajat kepadatan sel mast dengan derajatkepadatan saraf enterik yang masing-masing dikelompokkan menjadi 4 derajat, padalapisan submukosa dan muskularis, menggunakan uji Sommers'd. Hasil: Kepadatansel mast/lpb lebih tinggi pada apendisitis akut fokal (3,9±1,3) dibandingkanapendisitis akut supuratif-gangrenosa. Sedangkan kepadatan jaringan saraf enterik/lpblebih tinggi pada apendisitis akut supuratif-gangrenosa (3,7±0,9). Terdapat korelasikuat antara derajat kepadatan sel mast dengan derajat kepadatan jaringan saraf enterikpada lapisan muskularis apendisitis akut (p<0,05; r=0,733). Sedangkan pada lapisansubmukosa terdapat korelasi lemah antara kedua variabel tersebut (p>0,05; r=0,118).Tidak terdapat perbedaan kepadatan sel mast dan kepadatan jaringan saraf yangbermakna pada kelompok apendisitis akut (p>0,05). Kesimpulan: Kepadatan selmast tertinggi terdapat pada apendisitis akut fokal, sedangkan kepadatan jaringansaraf tertinggi pada apendisitis akut supuratif-gangrenosa. Terdapat korelasi kuatantara derajat kepadatan sel mast dengan derajat kepadatan jaringan saraf enterikpada lapisan muskularis, sedangkan korelasi lemah terdapat pada lapisan submukosa apendisitis akut.

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Background: Histopathologic examination is the gold standard for diagnosis of acute

appendicitis, although no obvious histopathological signs of acute inflamation shown.

Therefore other unknown pathogenesis is suspected. Several studies prove there is

correlation between mast cells and enteric nerve system on acute appendicitis. The

aims of this study are to see the density of mast cell and enteric nerve and to evaluate

correlation between grade of mast cell density and enteric nerve density on

histopathologically acute appendicitis. Material and methods: A cross-sectional

retrospective study was conducted on 97 histopathologically acute appendicitis which

grouped as acute focal, acute suppurative, gangrenous (phlegmonous) and

perforative. All sections were subjected to toluidine blue stain for mast cell and S100

stain for enteric nerve. The density of mast cell and enteric nerve were designed into

4 grades. A correlation test between grade of mast cell density and grade of enteric

nerve density were studied in submucosa and muscularis using Somers?d correlation

test. Results: The highest densities of mast cell/hpf (3,9±1,3) and enteric nerve/hpf

(3,7±0,9) were found in acute focal appendicitis and suppurative-gangrenous

appendicitis respectively. There was strong correlation between grade of mast cell

density and enteric nerve density in muscularis (p<0,05; r=0,733), whereas the

submucosal layer had the weak one (p>0,05; r=0,118). There was no significant

difference for mast cell and enteric nerve density on each group (p>0,05).

Conclusion: The highest densities of mast cell and enteric nerve were found in acute

focal appendicitis and suppurative-gangrenous appendicitis respectively. There was

strong correlation between grade of mast cell density and grade of enteric nerve

density in muscularis layer of acute appendicitis, meanwhile the weak correlation was

on submucosa.