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Studi pembentukan DNA adduct 8 hidroksi-2'-deoksiguanosin (8-OHdG) secara in vitro melalui reaksi fenton-like dengan butylated hydroxytoluene quinon (BHT-quinon) = In vitro study of DNA adduct hydroxyl-2'-deoxiguanosin (8-OHdG) formation through fenton-like reaction with butylated hydroxytoluene quinon (BHT-quinon)

Siti Hannaa; Budiawan, supervisor; Sri Handayani, supervisor; Siswati Setiasih, examiner; Riswijanto, examiner; Asep Saefumillah, examiner ([Publisher not identified] , 2016)

 Abstrak

ABSTRAK
Pada penelitian ini dilakukan studi in vitro pembentukan DNA adduct 8-hidroksi-2 deoksiguanosin (8-OHdG) sebagai biomarker kerusakan DNA, dengan mereaksikan 2 deoksiguanosin dengan BHT-Quinon melalui reaksi Fenton-like (Cr(III) dan H2O2). Reaksi dilakukan dengan variasi pH (7,4 dan pH 8,4), suhu (37 C dan 60 C), serta waktu inkubasi (7 dan 12 jam). 8-OHdG yang dihasilkan dianalisis menggunakan HPLC fasa terbalik dengan detektor UV-Vis. Hasil penelitian menunjukkan bahwa konsentrasi DNA adduct 8-OHdG yang paling tinggi diperoleh dari reaksi Fenton-like. Kesimpulan yang didapatkan ialah reaksi 2 dG, BHT-Q, Cr(III), dan H2O2 dihasilkan lebih besar dibanding reaksi 2 dG, BHT-Q, dan Cr(III) serta 2 dG, BHT-Q, dan Cr(III) dihasilkan lebih besar dibanding reaksi 2 -dG, BHT-Q dan H2O2 serta reaksi 2 dG + BHT-Q. Pembentukan DNA adduct 8-OHdG pada pH 8,4 lebih tinggi dibandingkan pH 7,4. Selain itu pembentukan DNA adduct 8-OHdG pada suhu 60 C juga lebih tinggi dibandingkan suhu 37 C dan pembentukan DNA adduct 8-OHdG dengan waktu inkubasi 12 jam lebih tinggi dibanding waktu inkubasi 7 jam.

ABSTRACT
In this research, in vitro study of DNA adduct 8-hidroxy-2 deoxyguanosine (8-OHdG) formation as biomarkers of DNA damage was conducted by reacting 2 -deoxyguanosine (2 dG) with BHT-Q through Fenton-like reaction (Cr(III) dan H2O2). The conditions of reactions were variated in pH (7,4 and pH 8,4), temperature (37 C and 60 C) and incubation time (7 and 12 hours). The 8-OHdG produce were analyzed by using reverse phase HPLC with UV-Vis detector. The result showed that 8-OHdG produced from reaction between 2 dG, BHT-Q, Cr(III), and H2O2 was higher than reaction between 2 dG, BHT-Q, and Cr(III). It also showed that reaction between 2 dG, BHT-Q, and Cr(III) produced 8-OHdG higher than reaction between 2 -dG, BHT-Q dan H2O2 also reaction of 2 dG with BHT-Q. The highest 8-OHdG formation obtained from Fenton-like reaction. The formation of DNA Adduct 8-OHdG at pH 8.4 was higher than pH 7.4. Meanwhile DNA adduct 8-OHdG formation at the temperature of 60 C was also higher than 37 C and DNA adduct formation of 8-OHdG at 12 hours incubation time is higher than 7 hours.

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 Metadata

Jenis Koleksi : UI - Skripsi Membership
No. Panggil : S69871
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Program Studi :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2016
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated (rdamedia ); computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : xv, 50 pages : illustration ; 30 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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No. Panggil No. Barkod Ketersediaan
S69871 14-19-794735148 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20464699
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