Artikel Jurnal :: Kembali

Artikel Jurnal :: Kembali

Detection of three pathogenic vibrio species using multiplex polymerase chain reaction (multiplex-PCR) / Umaporn Khimmakthong, Supaporn Noochu

Umaporn Khimmakthong; Supaporn Noochu (Thammasat University, 2017 )

 Abstrak

ABSTRACT
Vibrio cholera, Vibrio parahaemolyticus and Vibrio vulnificus are opportunistic pathogens causing disease in weak shrimp and possible food poisoning in shrimp consumers. In this study, three pairs of primers were designed to amplify the target DNA fragments of three Vibrio spp. and together with one pair for internal amplification control. The PCR condition was optimized to detect three Vibrio spp. in one reaction tube. The specificity and sensitivity of the reaction were evaluated. The results showed that this technique can detect V. cholera, V. parahaemolyticus and V. vulnificus in the same reaction tube with high specificity. Sensitivity is moderate, 0.5 ng-10 pg. In the future, this technique can be used to detect these bacteria in shrimp. It is potentially useful for shrimp farmers and shrimp consumers.

 Kata Kunci

 Metadata

Jenis Koleksi : Artikel Jurnal
No. Panggil : 607 STA 22:4 (2017)
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Subjek :
Penerbitan : Pathum Thani: Thammasat University, 2017
Sumber Pengatalogan : LibUI eng rda
ISSN : 25869000
Majalah/Jurnal : Science & Technology ASIA
Volume : Vol. 22 No. 4, Oct-Dec 2017: Hal. 11 - 18
Tipe Konten : text
Tipe Media : unmediated
Tipe Carrier : volume
Akses Elektronik :
Institusi Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 4, R. Koleksi Jurnal
  • Ketersediaan
  • Ulasan
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No. Panggil No. Barkod Ketersediaan
607 STA 22:4 (2017) 03-18-127058418 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20470943
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