This study is intended to investigate the correlation between religiousity and respondent's knowledge of prophet Muhammad's practice on tolerance (tasamuh) over Tasamuh, and the role of respondent's knowledge of prophet Muhammad's practice on tolerance as a mediating variable that can improve the relation between religiousity and tasamuh. Tasamuh can be defined as a religious tolerance in Islamic perspective. The research method that used to investigate that relationship and the role of respondent's knowledge of prophet Muhammad's practice on tolerance are bivariate correlation and path analysis. The result of the study shows that there is a negative correlation between relagiousity and tasamuh with coefficient correlation -0,374. Other results also show that relationship between religiousity and respondent's knowledge of prophet Muhammad's practice on tolerance is identified negatively with -,120 coefficient correlation.