Latar Belakang: Temulawak (Curcuma xanthorrhiza Roxb.) merupakan salah satu tanaman obat unggul Indonesia yang memiliki potensi untuk menghambat pembentukan biofilm C. albicans. Faktor virulensi yang dapat menyebabkan C. albicans menjadi fungi patogen diantaranya adalah pembentukan biofilm dan sekresi enzim hidrolitik. Fosfolipase merupakan salah satu enzim hidrolitik yang dapat merusak membran sel inang.Tujuan: Menganalisis aktivitas fosfolipase pada biofilm C. albicans ATCC 10231 fase awal, menengah, dan maturasi yang terhambat ekstrak etanol temulawak (Curcuma xanthorrhiza Roxb.).Metode: Nilai Kadar Hambat Biofilm Minimal (KHBM50) C. albicans ditentukan dengan uji MTT-assay. Ekstrak etanol temulawak dengan konsentrasi sesuai KHBM50 dipaparkan pada biofilm fase awal, menengah, dan maturase. Kontrol negative tidak dipaparkan apapun, kontrol positif dipaparkan Nystatin 100.000 IU. Aktivitas fosfolipase biofilm C. albicans dianalisis dengan mengukur proporsi antara diameterzona presipitasi dengan diameter koloni C. albicans pada medium Egg Yolk Agar (EYA).Hasil: Nilai KHBM50 ekstrak etanol temulawak terhadap biofilm C. albicans ATCC 10231 pada fase awal, fase menengah, dan fase maturasi berturut-turut adalah 25%, 30%, dan 35%. Pada kontrol positif, aktivitas fosfolipase biofilm C. albicans fase awal, fase menengah, dan fase maturasi bernilai 1. Aktivitas fosfolipase biofilm C. albicansfase awal, fase menengah, dan fase maturasi yang terhambat ekstrak etanol temulawak berturut-turut 0.84, 0.80, dan 0.83. Pada kontrol negatif, aktivitas enzim fosfolipase biofilm C. albicans fase awal, fase menengah, dan fase maturasi berturut-turut 0.59, 0.57, dan 0.57.Kesimpulan: Terdapat kecenderungan penurunan aktivitas enzim fosfolipase pada biofilm C. albicans yang terhambat > 50% ekstrak etanol temulawak.

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Background: Javanese turmeric (Curcuma xanthorrhiza Roxb.) is one of medical plant from Indonesia that has potency to inhibit biofilm formation of C. albicans. Biofilm formation and hydrolyticenzymes are two among manyvirulence factors of C. albicans. Phospholipaseisone of hydrolyticenzymesthat could degrade the hostcell membrane.Objective: To observe the activities ofphospholipase in early phase, intermediate phase, and maturation phase of biofilm C. albicans ATCC 10231 that has been inhibited by Javanese turmeric ethanolic extract.Method: MTT-assay wasused to measure the minimum biofilm inhibitory concentration (MBIC50) of C. albicans ATCC 10231in three phases of C. albicans biofilm. Those concentrations were used to observe phospholipase activities of biofilm in the relevant phases. The negative control were not exposed to anything, while the positive control were exposed to Nystatin 100.000 IU. Phospholipase activities were determined bymeasuring the proportion of precipitation zone diameter and C. albicans colony diameter onan egg yolk-agar medium.Results: The MBIC50of Javanese turmeric ethanolic extract towards formation of C. albicans biofilm ATCC 10231 in early phase, intermediate phase, and maturation phase were 25%, 30%, and 35%, respectively. Phospholipase activities value in early phase, intermediate phase, and maturation phase of C. albicans biofilm exposed by Nystatin were 1. Phospholipase activities value in early phase, intermediate phase, and maturation phase of C. albicans biofilms exposed by Javanese turmeric ethanolic extract were 0.84, 0.80, and 0.83, respectively. Phospholipase activities value in early phase, intermediate phase, and maturation phase of unexposed C. albicans biofilm were 0.59, 0.57, and 0.57, respectively.Conclusion: There istendency of decreased phospholipase activity in early phase, intermediate phase, and maturation phase of biofilm C. albicans that has been inhibited by Javanese turmericethanolic extract.