Infeksi HIV terus bertambah. Angka kesakitan dan angka kematian infeksi HIV dapat dihambat dengan terapi ARV. Terapi ARV efektif menekan viral load namun dapat menimbulkan dampak ketidaknyamanan. Kenyamanan spiritual merupakan aspek sentral untuk kesehatan. Pengkajian kenyamanan spiritual digunakan untuk menentukan masalah keperawatan. Penelitian ini ditujukan untuk membentuk instrumen kenyamanan spiritual pada pasien HIV positif. Metode penelitian menggunakan mixed methode. Hasil penelitian kenyamanan spiritual pasien HIV terdapat tiga faktor kenyamanan spiritual yaitu intrapersonal, interpersonal dan transpersonal. Instrumen kenyamanan spiritual pasien HIV positif valid dan reliabel untuk digunakan. Hasil respon regulator tekanan darah sistolik dan denyut jantung berhubungan dengan kenyamanan spiritual dengan tekanan darah sistolik dapat mendeteksi kenyamanan spiritual pada faktor intrapersonal (sensitivitas 70,8%), faktor interpersonal (spesivisitas 70,4%) dan faktor transpersonal (spesivisitas 81,5%). Uji diagnostik perubahan denyut jantung dapat mendeteksi kenyamanan spiritual pada faktor intrapersonal (sensitivitas 60%), faktor interpersonal (spesivisitas 61,5%) dan faktor transpersonal (spesivisitas 80,8%).

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The infection of HIV increases continuously with morbidity and mortality rates can be inhibited through the provision of ARV therapy. Giving ARV therapy suppresses effectively the viral load even though it has an impact on patients' discomfort. However, spiritual comfort is a central aspect in health care. Therefore, spiritual comfort needs to be determine nursing problems. This research intended to produce a spiritual comfort instrument to patients with HIV positive. The research used a mixed-method. The HIV patients' spiritual comfort consisted of three factors which are intrapersonal, interpersonal and transpersonal. The instrument of spiritual comfort for patients with HIV positive valid and reliable to use.  The regulator response of systolic blood pressure and heart rate related to the spiritual comfort. The systolic blood pressure detect the spiritual comfort for intrapersonal factor 70.8% sensitivity, interpersonal factor 70.4% specificity, and transpersonal factor 81.5% specificity. The heart rate changes in diagnostic test detect spiritual comfort in intrapersonal factor 60% sensitivity, interpersonal factor 61.5% specificity, and transpersonal factor 80.8% specificity.