Tuberkulosis (TB) sampai dengan saat ini masih merupakan salah satu masalah kesehatan
utama di Indonesia. Sepuluh persen dari TB ekstraparu adalah TB tulang, dan sekitar 50%
penderita TB tulang menyerang tulang belakang (Spinal Tuberkulosis). Respon tubuh
terhadap Mycobacterium tuberculosis (M.tb) sehingga menimbulkan penyebaran
ekstraparu, khususnya respon makrofag sebagai pertahanan lini pertama, masih belum
sepenuhnya dimengerti. Makrofag menghasilkan molekul reactive oxygen species (ROS)
sebagai hasil dari oxygen burst untuk mengeliminasi antigen. Nitrat Oksida (NO) dan
mieloperoksidase (MPO) berperan pada oxygen burst Selain itu, Pada fagositosis
terdapat organel lisosom yang di dalamnya terdapat enzim hidrolase (fosfatase asam dan
beta glukuronidase) berguna pada pencernaan intraseluler. Penelitian ini menguji
hipotesis bahwa ada gangguan fungsi makrofag pada pasien TB tulang belakang. Monosit
diisolasi dari peripheral blood mononuclear cells (PBMC) dari lima pasien TB tulang
belakang dan lima orang sehat sebagai kontrol. Monosit yang terisolasi dikultur dengan
stimulasi dari macrophage colony-stimulating factor (M-CSF) selama tujuh hari untuk
pematangan. Kemampuan fagositosis makrofag dinilai aktivitasnya terhadapa sel darah
merah domba (SDMD). Sedangkan nitrat oksida (NO), mieloperoksidase (MPO), betaglukuronidase,
dan aktivitas fosfatase asam diselidiki dengan metode spektrofotometer.
Analisis data dengan menggunakan aplikasi SPSS versi 20. Kami menemukan bahwa
monosit yang diisolasi dari PBMC pasien TB tulang belakang secara signifikan lebih
sedikit dibandingkan dengan kelompok kontrol (2992.103 vs 6474.103 (sel / mL)) dan
juga lebih sedikit makrofag yang melekat pada sel darah merah domba (SDMD) (264.103
vs 598.103 (sel / mL)). Namun, produksi NO (2346 vs 325,17 (μmol / gram protein)), dan
MPO (570,7 vs 17,4 (unit / mg), beta-glukuronide (0,149 vs 0,123 (unit / mg)), dan asam
fosfatase aktivitas (1776,9 vs 287,9 (unit / mg)) dari makrofag kelompok TB tulang
belakang lebih tinggi daripada kelompok yang sehat serta korelasi negatif kuat dan
bermakna antara fagositosis makrofag dengan tiap variabel tersebut. Selain itu, Terdapat
korelasi positif lemah dan tidak bermakna antara kejadian fagositosis dan uji WST.
Meskipun pengenalan rendah pada benda asing, proses makrofag intraseluler, termasuk
aktivitas oksidatif dan fungsi lisosom, tinggi secara signifikan. Hasil ini menunjukan
penurunan fungsi makrofag pada pasien TB tulang belakang serta terdapat kemungkinan
adanya dominasi imunitas non-spesifik bawaan pada infeksi TB tulang belakang
Tuberculosis (TB) is still one of the main health problems in Indonesia. Ten percent of
extrapulmonary TB is bone TB and about 50% of people with bone TB affected to the
spine. The immune response against Mycobacterium tuberculosis (M.tb), which causes
extrapulmonary spread, particularly the response of macrophages as a first-line defense,
is still not fully understood. Macrophages produce reactive oxygen species (ROS)
molecules as a result of oxygen bursts to eliminate antigens. Nitric Oxide (NO) and
myeloperoxidase (MPO) play a role in oxygen burst. Also, phagocytosis process involved
lysosomal organelles in which there are hydrolase enzymes (acid phosphatase and betaglucuronidase),
which have important role in intracellular digestion. This study examined
the hypothesis about impairment of macrophage function in spondylitis TB patients.
Monocytes were isolated from peripheral blood mononuclear cells (PBMC) collected
from five spinal TB patients and five healthy people as controls. Isolated monocytes were
cultured by stimulation of macrophage colony-stimulating factor (M-CSF) for seven days
for maturation. The phagocytic ability of macrophages is assessed as to their activity on
sheep red blood cells. Whereas nitric oxide (NO), myeloperoxidase (MPO), betaglucuronidase,
and acid phosphatase activity were investigated by spectrophotometer
methods. Data was analyzed using SPSS version 20. We found that monocytes isolated
from PBMC of spondylitis TB patients were significantly less than in the control group
(2992,103 vs 6474,103 (cells / mL)) and also fewer macrophages attached to red blood
cells sheep (264,103 vs 598,103 (cells / mL)). However, NO production (2346 vs 325.17
(μmol / gram protein)), MPO (570.7 vs. 17.4 (units/mg), beta-glucuronide (0.149 vs 0.123
(units/mg)), and acids phosphatase activity (1776.9 vs 287.9 (units/mg)) of macrophages
in the spondylitis TB group were higher than in the healthy group. There was a strong
and significant negative correlation between phagocytosis of macrophages with each of
the previous variables. There was no significant difference between phagocytic ability
and the WST test. Although the recognition against foreign bodies was low, intracellular
macrophage processes, including oxidative activity and lysosomal function, were
significantly higher than control. This result showed a decrease of macrophage function
in spondylitis TB patients as well as a possible dominance of non- specific immunity.
T-Muhamad Dwi Putra.pdf :: Unduh