AbstrakMen who have sex with men (MSM) comprise a population at risk for HIV infection. Assessing the Quality of Life (QOL) in MSM might be different than other populations. This study showed a secondary analysis from our previous research. It was needed to understand whether peer support and family acceptance had an impact on QOL of MSM living with HIV and ART (Antiretroviral Therapy). A total of 175 respondents were involved in this cross-sectional study that was carried out with purposive sampling. The questionnaires were translated to Bahasa and tested for validity and reliability. Data questionnaires completed were analyzed. Results showed that peer support was positively correlated with QOL (p= 0.023; OR= 2.070), and also, family acceptance was significantly related to QOL (p= 0.001; OR= 2.766). Thus, peer support and family acceptance are important factors affecting the well-being and QOL of MSM living with HIV and ART. This finding can be used for the improvement of QOL in people living with HIV.