Latar belakang: Sebagai upaya pengembangan rekayasa jaringan tulang untukpenggantian kerusakan jaringan yang luas, telah dikembangkan rekayasa jaringanmaterial graft. Material rekayasa jaringan tulang harus menyerupai komposisi tulang asli,oleh karena itu saat ini scaffold berbahan dasar hidroksiapatit-gelatin banyak diteliti dandisukai penggunaannya karena bersifat biomimetik, biokompatibel, serta biodegrable.Propolis merupakan bahan alami yang diproduksi lebah yang sudah terbukti dapatmendukung dan mempercepat regenerasi tulang. Oleh karena itu, penambahan propolissebagai bahan bioaktif diharapkan dapat yang meningkatkan biokompatibilitas bonegraft. Penambahan suatu zat terhadap suatu material medis diperlukan uji keamanan danpengaruhnya, salah satu uji keamanan adalah uji sitotoksisitas terhadap sel yang mungkinberperan dalam regenerasi tulang untuk mengetahui biokompatibilitasnya. Tujuan:Membuat literature review mengenai potensi penambahan propolis dalam bone grafthidroksiapatit-gelatin serta pengaruhnya terhadap viabilitas sel punca mesenkimal(mesenchymal stem cell) dalam berbagai literatur terkait. Metode: Penyusunan literaturereview ini dilakukan pada bulan Desember 2020 dengan melakukan pencarian literaturterkait melalui 2 electronic database, yaitu PubMed dan Scopus dengan menggunakankombinasi kata kunci terkait. Penentuan literatur terpilih sesuai kriteria inklusi dilakukandengan mengikuti alur Preferred Reporting Items fot Systematic Reviews and Meta-Analyses(PRISMA) Hasil:Dari hasil penelusuran pustaka maka terpilih 10 literatur. Dualiteratur menyatakan bahwa propolis dalam konsentrasi 10% dan 20% tidak bersifatsitotoksik dan propolis 10% terbukti dapat meningkatkan adhesi dan proliferasi sel puncamesenkiman. Delapan literatur menyatakan bahwa bone graft berbahan dasarhidroksiapatit dan gelatin tidak bersifat sitotoksik. Kesimpulan: Bone grafthidroksiapatit-gelatin dan propolis tidak bersifat sitotoksik terhadap sel puncamesenkimal.

---

materials using bone tissue engineering have been developed. Materials that is used in

bone tissue engineering should be similar to the original bone composition, therefore

scaffold based on hydroxyapatite-gelatin is currently widely researched and used because

it is biomemetic, biocompatible, and biodegradable. Propolis is a natural ingredient

produced by bees that has been proven accelerated bone regeneration. Therefore, it is

expected that the addition of propolis as a bioactive material is expected to increase the

biocompatibility of bone grafts. The addition of a substance to a medical material for

should go through efficacy and safety testing. One of the safety tests is the cytotoxicity

test of the material towards cells that may play a role in bone regeneration to see its

biocompatibility. Objective: A literature review on the potential of the addition of

propolis to hydroxyapatite-gelatin bone graft and its effect on the viability of

mesenchymal stem cell. Methods: The literature review was conducted in December

2020 by searching for related literature through 2 electronic databases, namely PubMed

and Scopus using a combination of keywords that match research question. The

determination of selected literature according to inclusion criteria was carried out by

following the flow of Preferred Reporting Items for Systematic Reviews and Meta-

Analyzes (PRISMA). Results: From the literature search results, 10 literatures were

selected. Two literatures state that 10% and 20% propolis in concentrations are not

cytotoxic. Ten percent propolis has been shown to increase adhesion and proliferation of

mesenchymal stem cells, and eight literatures state that bone grafts which are based on

hydroxyapatite-gelatin are not cytotoxic. Conclusion: Hydroxyapatite-gelatin and

propolis bone graft are not cytotoxic to mesenchymal stem cells.