Pendahuluan: Diabetes melitus (DM) merupakan gangguan metabolik kronik yangditandai dengan hiperglikemia. Salah satu komplikasi DM adalah disfungsi ginjal. Daunsirsak (Annona muricata) memiliki potensi sebagai antidiabetes. Namun, belum adapenelitian mengenai ekstrak etanol daun Annona muricata terhadap morfologi ginjalterutama terkait proliferasi dan pertumbuhan dari sel mesangial glomerulus, ruangBowman, dan tubulus kontortus proksimal. Penelitian ini bertujuan untuk mengetahuipengaruh ekstrak etanol daun Annona muricata terhadap perubahan histopatologi ginjaldari mencit Swiss Webster yang diinduksi Aloksan. Metode: Penelitian ini menggunakanstudi eksperimental in vivo. Sampel penelitian berasal dari organ ginjal 30 ekor mencitjantan galur Swiss Webster yang diinduksi aloksan. Sampel dibagi menjadi kelompokkontrol negatif, kontrol positif, dan 3 kelompok perlakuan yang diberikan ekstrak etanoldaun sirsak (EEDS) dengan masing-masing dosis 150, 300, dan 600 mg/kgBB selama 14hari. Semua sampel preparat jaringan ginjal diwarnai dengan pewarnaan PAS dan diamatipada perbesaran 400x dengan melihat pada 10 LP. Pengukuran histopatologi dilakukandengan software MIimageView. Hasil: EEDS dalam semua dosis perlakuan tidakberpengaruh secara signifikan pada diameter glomerulus dan tebal tubulus kontortusginjal serta lebar ruang bowman ginjal kiri dan tubularisasi glomerulus ginjal kanan.EEDS dosis 600 mg/kgBB berpengaruh secara signifikan (p<0,05) pada lebar ruangbowman ginjal kanan. EEDS dosis 600 mg/kgBB dan kontrol positif secara signifikan(p<0,05) dapat mencegah tubularisasi glomerulus ginjal kiri. Kesimpulan: PemberianEEDS selama 14 hari tidak berpengaruh pada ukuran diameter glomerulus dan tebaltubulus kontortus proksimal ginjal kiri dan kanan serta lebar ruang bowman ginjal kiridan tubularisasi glomerulus ginjal kanan. Namun, pemberian EEDS dosis 600 mg/kgBBdapat mencegah penyempitan ruang bowman ginjal kanan dan tubularisasi glomerulusginjal kiri.

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Introduction: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by

hyperglycemia, one complication of which includes kidney disfunction. Soursop leaves

(Annona muricata) is known to have antidiabetic potential; however, no research has been

conducted regarding the effect of ethanol extract of Annona muricata leaves on the

morphology of the kidney, particularly the proliferation and growth of the mesangial cells

of glomerulus, Bowman’s space, and the proximal convoluted tubule. This study aimed

to observe the effect of Annona muricata leaves ethanol extract on the morphological

chanes of Alloxan-Induced Swiss Webster Mice’s Kidney. Method: This study was

conducted as an in vivo experimental study. The samples were obtained from the kidneys

of 30 alloxan-induced male Swiss Webster mice, which were then grouped into negative

control, positive control, and 3 experimental groups given ethanol extracts of soursop

leaves (EEDS) with a dose of 150, 300, and 600 mg/kgBW for 14 days. The kidney tissue

samples were stained using the PAS staining and observed with a 400x magnification on

10 LP. The histopathological measurement was conducted using the MIimageView

software. Results: EEDS in all dose of treatment showed no significance on the

glomerular diameter and the thickness of the proximal convoluted tubules of the kidney,

as well as the width of the Bowman’s space of the left kidney and glomerular

tubularization of the right kidney. EEDS with a dose of 600 mg/kgBW showed a

significant effect (p<0.05) on the height of the right kidney’s Bowman’s space. EEDS

with a dose of 600 mg/kgBW and the positive control significantly (p<0.05) prevented

the tubularization of the left kidney’s glomerulus. Conclusion: The 14 days treatment

with EEDS didn’t have a significant difference on the glomerular diameter and the

thickness of the proximal convoluted tubules of the left and right kidney, as well as the

height of the Bowman’s space of the left kidney and the right kidney’s glomerular

tubularization. However, EEDS with a dose of 600 mg/kgBW could prevent the

narrowing of the Bowman’s space of the right kidney and the glomerular tubularization

of the left kidney.