Pendahuluan: Mikoorganisme mulut sangat penting dalam menjaga kesehatan mulutdan sistemik. Salah satu dari berbagai macam mikroorganisme dalam mulut adalah bakteriSolobacterium moorei. Perlekatan bakteri pada seluruh rongga mulut ini menyebabkanterjadinya kolonisasi bakteri pada permukaan mulut dan membentuk biofilm. Selain itu, proteinpada saliva merupakan faktor penting yang mempengaruhi pembentukan biofilm dan interaksiantar spesies. Profil protein saliva dalam pembentukan biofilm setiap individu dapat bervariasisecara kualitatif dalam kondisi yang berbeda. Tujuan: Mengamati pengaruh pajanan proteinsaliva anak dan kelompok dewasa terhadap pembentukan biofilm Solobacterium moorei.Metode: Sampel subjek anak dan dewasa, dilakukan uji Eletroforesis untuk mengetahui profilprotein pada saliva, uji Crystal Violet untuk menentukan kuantifikasi pembentukan biofilm danTotal Plate Counting untuk mengkuantifikasi viabilitas bakteri pada massa biofilm. Hasil:Tidak terdapat perbedaan bermakna pada subjek anak dan dewasa teridentifikasi proteindengan berat molekul berkisar sama yaitu sebesar 10-15 kDa dan 50 kDa. Berdasarkan hasilpenelitian, kolonisasi bakteri pada rongga mulut oleh biofilm Solobacterium moorei pada anakdan dewasa tidak dipengaruhi oleh Profil Protein. Profil protein saliva diuji Eletroforesisdinyatakan tidak ada perbedaan komponen antara anak dan dewasa yang menjadi indikatoryang mempengaruhi pertumbuhan biofilm bakteri Solobacterium moorei. Akan tetapi, secarastatistik terbukti nyata jumlah koloni bakteri dewasa lebih banyak dibandingkan dengan anak.Kesimpulan: Profil protein saliva tidak mempengaruhi pembentukkan biofilm bakteriSolobacterium moorei. Tidak ditemukan perbedaan pada komponen profil protein pada dewasamaupun anak. Hasil analisis secara statistik membuktikan tidak ada perbedaan bermakna padapembentukan biofilm Solobacterium moorei.

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Background: Oral microorganisms plays a huge role in maintaining oral and systemic

health. One of the various types of microorganisms in the mouth is the bacterium

Solobacterium moorei. The attachment of bacteria to the entire oral cavity causes bacterial

colonization on the surface of the mouth and forms a biofilm. In addition, protein in saliva is

an important factor in biofilm formation and species interactions. The salivary protein profile

in the biofilm formation of each individual can vary qualitatively under different conditions.

Objective: To observe the effect of exposure to pellicle formation of salivary protein groups

of children and adults on the formation of biofilm Solobacterium moorei. Methods: Samples

of children and adults, Electrophoresis test was performed to determine the protein profile in

saliva, Crystal Violet test to determine the quantification of biofilm formation and Total Plate

Counting to quantify bacterial population viability at the mass of biofilm. Results: There were

no significant differences in children and adults, identified proteins with the same molecular

weight, namely 10-15 kDa and 50 kDa. Based on the research results, bacterial colonization on

the surface of the mouth by Solobacterium moorei bacteria in children and adults was not

influenced by the protein profile. The salivary protein profile was tested by electrophoresis, it

was stated that there was no difference in components between children and adults which was

an indicator that influenced the growth of the Solobacterium moorei bacterial biofilm.

However, it is statistically proven that the number of adult bacterial colonies is larger than

children’s. Conclusion: Salivary protein profile does not influences the biofilm formation of

Solobacterium moorei bacteria. There were no differences in protein profile components

between adults and children. The results of statistical analysis proved that there was no

significant difference in the formation of the Solobacterium moorei monospecies biofilm.