Penyakit infeksi fungi merupakan penyakit yang sering ditemukan di Indonesia. Pencarian antibiotik yang tepat untuk penyakit infeksi fungi masih terus dilakukan. Pseudomonas azotoformans UICC B-91 berpotensi sebagai antimikroba. Tujuan penelitian untuk mengetahui mekanisme penghambatan metabolit P. azotoformans UICC B-91 dari medium terhadap fungi patogen secara morfologi. Hasil penelitian menunjukkan senyawa metabolit P. azotoformans UICC B-91 mampu menghambat C. albicans ATCC 10231 dan T. mentagrophytes. Zona inhibisi larutan medium P. azotoformans UICC B-91 terhadap C. albicans ATCC 10231 konsentrasi 100 mg/mL dan 80 mg/mL memiliki daya hambat kuat, konsentrasi 60 mg/mL memiliki daya hambat sedang. Zona inhibisi terhadap T. mentagrophytes konsentrasi 100 mg/mL dan 80 mg/mL memiliki daya hambat kuat, konsentrasi 60 mg/mL memiliki daya hambat sedang. Pengamatan mikroskopis C. albicans ATCC 10231 setelah penambahan medium P. azotoformans UICC B-91 di bawah Scanning Electron Microscope (SEM) mengalami perubahan permukaan sel yeast menjadi tidak rata. Pengamatan mikroskopis T. mentagrophytes setelah penambahan medium P. azotoformans UICC B-91 mengalami konstriksi mikrokonidia. Mekanisme penghambatan metabolit dari medium P. azotoformans UICC B-91 terhadap C. albicans ATCC 10231 diduga melalui difusi yang mengganggu fungsi membran sel atau menghambat transisi bentuk yeast ke bentuk hifa, sedangkan terhadap T. mentagrophytes diduga mengganggu dinding sel dan mengganggu fungsi membran sel.

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Fungal infection is a disease that is often found in Indonesia. The search for appropriate antibiotics for fungal infections is still ongoing. Pseudomonas azotoformans UICC B-91 has potential as an antimicrobial. The purpose of this study was to determine the mechanism of inhibition of the metabolite of P. azotoformans UICC B-91 from the medium against morphologically pathogenic fungi. The results showed that the metabolite compound P. azotoformans UICC B-91 was able to inhibit C. albicans ATCC 10231 and T. mentagrophytes. Inhibition zone of medium solution of P. azotoformans UICC B-91 against C. albicans ATCC 10231 with a concentration of 100 mg/mL and 80 mg/mL had a strong inhibitory effect, a concentration of 60 mg/mL had a moderate inhibitory effect. The zone of inhibition for T. mentagrophytes at concentrations of 100 mg/mL and 80 mg/mL had a strong inhibitory effect, a concentration of 60 mg/mL had moderate inhibition. Microscopic observation of C. albicans ATCC 10231 after the addition of P. azotoformans UICC B-91 medium under a Scanning Electron Microscope (SEM) changed the yeast cell surface to become uneven. Microscopic observation of T. mentagrophytes after the addition of P. azotoformans UICC B-91 medium experienced constriction of microconidia. The mechanism of inhibition of metabolites from P. azotoformans UICC B-91 medium against C. albicans ATCC 10231 is thought to be through diffusion which disrupts cell membrane function or inhibits the transition from yeast to hyphal form, while against T. mentagrophytes it is thought to disrupt cell walls and disrupt cell membrane function.