Kayu manis (Cinnamomum burmannii B.) merupakan tanaman asli Indonesia yang biasa digunakan sebagai rempah dan obat tradisional. Perbedaan lokasi tumbuh C. burmannii di Indonesia memiliki potensi untuk memengaruhi senyawa bioaktifnya yang bermanfaat bagi kesehatan. Penelitian ini bertujuan untuk mengidentifikasi dan mengelompokkan metabolit ekstrak metanol kayu manis (C. burmannii), serta mengevaluasi aktivitas antioksidan dan toksisitas ekstrak C. burmannii dari lima provinsi di Indonesia. Senyawa metabolit dianalisis dengan teknik kromatografi cair-spektrometri massa (LC-MS). Kandungan total fenolik (TPC), kandungan total flavonoid (TFC), nilai antioksidan, dan nilai toksisitas secara berturut-turut diukur menggunakan metode Folin-Ciocalteu, kolorimetri AlCl3, 2-Diphenyl-1-picrylhidrazyl (DPPH), dan Brine Shrimp Lethality Test (BSLT). Hasil penelitian menunjukkan bahwa model Partial Least Squares Discriminant Analysis (PLS-DA) yang dibangun mengelompokkan sampel ke dalam tiga kelompok yang signifikan dengan nilai R2X (cum) = 0,775, R2Y (cum) = 0,992, dan Q2 (cum) = 0,875. Senyawa dengan ID N1543 dan N31 teridentifikasi sebagai senyawa yang terdapat di semua sampel. Ekstrak C. burmannii dari lokasi tumbuh yang berbeda memiliki kandungan total fenolik dan flavonoid secara berturut-turut sebesar 82,42 ± 3,4–316,26 ± 20,94 mg GAE/g dan 18,08 ± 0,23–96,10 ± 7,97 mg QE/g, serta aktivitas antioksidan dan toksisitas secara berturut-turut sebesar 43,85 ± 1,09–170,54 ± 24,61 µg/mL dan 341,95 ± 32,24–826,10 ± 57,79 µg/mL. Kandungan metabolit sekunder aktif dalam ekstrak kayu manis, yaitu senyawa fenolik dan flavonoid, ditemukan berkorelasi pada sifat antioksidannya.

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Cinnamon (Cinnamomum burmannii B.) is a native Indonesian plant commonly used as a spice and traditional medicine. The variations in the growing locations of C. burmannii in Indonesia can potentially affect its beneficial bioactive compounds. This study aims to identify and categorize the metabolites of cinnamon (C. burmannii) methanol extracts and evaluate the antioxidant activity and toxicity of C. burmanniiextracts from five provinces in Indonesia. The metabolite compounds were analyzed using Liquid Chromatography-Mass Spectrometry (LC-MS) technique. The total phenolic content (TPC), total flavonoid content (TFC), antioxidant value, and toxicity value were measured using Folin-Ciocalteu method, AlCl3 colorimetric assays, 2-Diphenyl-1-picrylhidrazyl (DPPH), and Brine Shrimp Lethality Test (BSLT), respectively. The results showed that the constructed Partial Least Squares Discriminant Analysis (PLS-DA) model categorized the samples into three significant groups with R2X (cum) = 0.775, R2Y (cum) = 0.992, and Q2 (cum) = 0.875. The compound with ID N1543 and N31 were identified as a compound present in all samples. The C. burmannii extracts from different growing locations had total phenolic and flavonoid contents ranging from 82.42 ± 3.4–316.26 ± 20.94 mg GAE/g and 18.08 ± 0.23–96.10 ± 7.97 mg QE/g, as well as antioxidant activity and toxicity ranging from 43.85 ± 1.09–170.54 ± 24.61 µg/mL and 341.95 ± 32.24–826.10 ± 57.79 µg/mL, respectively. The presence of active secondary metabolites, such as phenolic and flavonoid compounds in cinnamon extracts correlated with its antioxidant properties.