Latar Belakang : Runt related transcription factor2 (RUNX2) memiliki peranpenting dalam proses osteoblastogenesis yang terlibat dalam pengaturan formasitulang. Penurunan aktivitas sel osteoblas dapat mengakibatkan terjadinyaosteoporosis. Pasien osteoporosis lebih banyak dialami oleh wanita menopauseyang diperlihatkan dengan penurunan kadar estrogen. Pada penelitian ini akanmenganalisis Single Nucleotide Polymorphism (SNPs) yang diamati pada daerahpromoter gen RUNX2 untuk mengetahui hubungan polimorfisme promoter 1 (P1)gen RUNX2 terhadap T-score dan risiko osteoporosis wanita menopause.Bahan dan Metode : Penelitian ini bertujuan untuk menganalisis polimorfismepada promoter 1 -330 G/T RUNX2 dengan desain penelitian cross-sectional. SNPsyang terdapat pada promoter (P1) RUNX2 dianalisis menggunakan metode PCRRFLPyang dilakukan pada 161 wanita menopause, terdiri dari 50 wanitaosteoporosis, 53 osteopenia dan 58 wanita normal. Densitas mineral tulang diukurmenggunakan nilai T-score subyek menggunakan teknik Quantitative Ultrasound(QUS).Hasil : Hasil penelitian menunjukkan bahwa distribusi genotip dan alotip padasemua kelompok (normal, osteopenia dan osteoporosis) tidak berbeda bermakna(P>0,05). T-score masing-masing genotip dan alotip secara statistik menunjukkanhasil yang tidak berbeda bermakna, namun perbandingan rerata T-score padawanita dengan homozigot alel T lebih rendah daripada homozigot alel G danheterozigot GT, selain itu frekuensi pada genotip TT dan alel T cenderungmeningkat pada kelompok osteoporosis. Analisis Odds Ratio (OR)memperlihatkan bahwa masing-masing genotip bukan merupakan faktor risikotehadap osteoporosis. Hasil penelitian ini dapat disimpulkan bahwa tidak terdapathubungan bermakna antara polimorfisme genetik promoter gen RUNX2 terhadaprisiko osteoporosis (P>0,05).

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Introduction : Runt related transcription factor2 (RUNX2) is considered to play

important role for osteoblastogenesis and involved in the regulation of bone

formation. Decreased activity of osteoblastic cell is one important factor causing

osteoporosis. Most patients suffering from osteoporosis are menopausal women

exhibiting decreased of estrogen. Single nucleotide polymorphism (SNPs) was

observed within RUNX2 promoter 1 (P1) to identify its association for T-score

and risk of osteoporosis in menopausal women.

Material and Methode : In the present study we analysed polymorphism in the

RUNX2 promoter 1 -330 G/T with the cross sectional design. SNPs within

RUNX2 Promoter 1 (P1) was examined using PCR-RFLP methode in 161

menopausal Indonesian women; 50 women with osteoporosis, 53 with osteopenia

and 58 healthy women. Bone mineral density (BMD) was measured by T-score

value using Quantitative Ultrasound (QUS).

Results : The result showed that the difference of genotype and alotype frequency

of RUNX2 promoter gene were not statistically significant. The T-score of

genotype and alotype were not significantly different (p>0,05). While women who

were homozygous allele (T) had lower average T-score than homozygous allele

(G) and heterozygous (GT). Genotype frequency of TT and allele T tend to

increase in osteoporosis group. The Odds Ratio showed genotype in RUNX2

promoter 1 had no risk of osteoporosis. These data suggest that polymorphism of

RUNX2 Promoter 1 was not assosiated to the risk of osteoporosis in Indonesian

menopausal women (p>0,05).