UI - Tesis Membership :: Kembali

UI - Tesis Membership :: Kembali

Respon IgG Spesiflk terhadap Antigen Gag HIV-1 pada Mencit yang Diimunisasi dengan Kandidat Vaksin Vp22-Gag = HIV-1 Gag Specific IgG Response in Mice Immunized with Vp22-Gag Vaccine Candidate

Heri Setiyo Bekti; Budiman Bela, supervisor; Silvia Tri Widyaningtyas, supervisor; Andi Yasmon, examiner; Heri Wibowo, examiner; Tania Meirianty (Fakultas Kedokteran Universitas Indonesia , 2017)

 Abstrak

Latar belakang : Salah satu strategi dalam mengkontrol infeksi HIV, yaitu dengan pengembangan kandidat vaksin dengan efektifitas yang baik. Salah satu komponen virus yang dapat dikembangkan sebagai vaksin adalah protein Gag, yang merupakan protein struktural virus dan bersifat realtif lebih lestari dibandingkan komponen protein virus yang lain. Stimulasi respon sel T ens+ spesifik Gag, terkait dengan penurunan viremia, kontrol replikasi virus, dan perkembangan penyakit yang lambat. Respon T ens+ yang efektif juga dipengaruhi oleh sel T CD4+. Protein rekombinan Gag dapat diklona dan diekpre
Background : Ones strategy for controlling HIV infection with developing a candidate vaccine, which has favorable effectiveness. Gag protein is one of the one of the viral components that can be developed· as a candidat vaccine, its a virion-building structural protein and relatively more converse. Generate response of specific Gag-CD8+ T cell associated with reduction in veremia, viral replication control, and slow disease progressione. Effective response of CD8+ T cell also influenced by CD4+ T cell. Gag recombinant protein can be cloned and expressed in the prokaryotic system, and when immunized to experimental animalas or human its will be as exogenous antigens. Exogenous antigens can become endogenous antigens by adding proteins that have the ability to translocate into cell membranes, one of which is the Vp22 protein. Methodology : Transformation of plasmid that encodes the Gag and Vp22-Gag recombinant proteins to prokaryotic expression system, followed by purification with Ni-NT A resin. Recombinant proteins which has purification, analyzed by SDS-PAGE and western blotting methods. Recombinant proteins are transfected to CHO cell to determine cellular migration ability. Immunization experimental animals with recombinant proteins to determine ability of generate Gag-specific IgG response. Results : Test of western blotting showed recombinant proteins interacted with rabbit antibodies against p24 antigens, which produces a band between protein ladder with moleculer weight 30 KDa and 50 KDa Observations with confocal microscopy showed Gag and Vp22-Gag recombinant proteins localized to endosomes, marked with the presence of yellow flourosence. Test of ELISA, showed Gag-specific IgG response after immunization experimental animal with recombinant proteins Conclusion : Gag and Vp22-Gag recombinant proteins can be expressed on a system of prokaryotic expression. Intraceluler migration of recombinant proteins on mamalian cell not be proven yet. Vp22-Gag recombinant protein can generated response Gag-specific lgG.

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Jenis Koleksi : UI - Tesis Membership
No. Panggil : T58359
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Program Studi :
Penerbitan : Jakarta: Fakultas Kedokteran Universitas Indonesia , 2017
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : xviii, 64 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, lantai 3
  • Ketersediaan
  • Ulasan
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No. Panggil No. Barkod Ketersediaan
T58359 15-24-63094135 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 9999920552885
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