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"Salah satu cara untuk merangsang pembungaan adalah dengan memberikan zat pengatur tumbuh. Dalam penelitian ini digunakan salah satu zat pengatur tumbuh yaitu Hydrasil yang diberikan dengan menyemprotkan larutan ke seluruh bagian tanaman setiap 10 hari dengan 4 kali pemberian.Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Hydrasil terhadap pertumbuhan vegetatif dan pembungaan anggrek Laeliocattleya. Konsentrasi yang digunakan mulai dari 0,75 ppm hingga 2,50 ppm dengan selang 0,25 ppm. Hasil penelitian menunjukkan bahwa pemberian Hydrasil berpengaruh nyata terhadap pertambahan lebar daun dan jumlah tunas anakan baru. Pemberian Hydrasil tidak berpengaruh nyata terhadap pertambahan panjang daun, luas daun tinggi tanaman, berat basah tanaman, dan belum mampu merangsang pembungaan.Hasil uji nonparametrik Friedman pada taraf nyata α = 0,05 menunjukkan bahwa pemberian Hydrasil 0,75 PPm memberi pengaruh paling baik terhadap pertambahan lebar daun. Pemberian Hydrasil 0,75 ppm berpengaruh paling baik terhadap pertambahan jumlah tunas anakan."

"ABSTRAKUkel, Crozier, or Fiddlehead is a term for a fern leaf bud with circinnate type. These structure is one of the distinctive character found in ferns. In this article, some aspects related to the fern leaf bud will be discussed, include the origin of local name, list of non-circinnate type, developmental process, morphological diversity and potential utilization. This article is expected to further increase the interest of researcher and utilize these attractive and unique structure."

"ABSTRAKCanangium odoratum Baill. dikenal dengan nama kenanga, banyak ditanam sebagai tanaman hias. Bunganya yang berbau harum dapat diekstraksi untuk diambil minyak atsiri. Kultur kalus telah banyak dicoba untuk mendapatkan berbagai jenis metabolit sekunder yang disintesa oleir tanaman.Pemberian zat pengatur tumbuh 2,4-D dan kinetin 0,5 1,5 ppm pada media murashige & Skoog 1962 yang dinerkaya dengan air kelapa 15% dan ekstrak khamir 0,2 ppm, merangsang pertumbuhan eksplan petal kenanga hingga terbentuk kalus. Pengamatan kualitatif dilakukan selama 55 hari dengan selang waktu 5 hari. Pengamatan kuantitatif yaitu menimbang berat kalus hari ke-55. Jenis kalus yang terbentuk friabel kompak dengan warna bervariasi antara lain: putih dan hiiau. Biomassa kalus terbesar diperoleh pada perlakuan dengan pemberian 2,4-D 1,5-pom/kinetin 1,O pmm, yaitu 1,3129 gram berat basah dengan berat kering 0,0525 gram. Berdasarkan hasil uji perbandingan berganda, biomassa kalus dengan pemberian 2,4-D 1, 5ppm / kinetin 1,0 ppm berbeda nyata dengan biomassa kalus pada pemberian 2,4-D O,5 ppm/kinetin 0,5 PPm. Kenaikan konsentrasi 2,4-D dan kinetin mempercepat pembentukan kalus, tetapi tidak selalu disertai dengan kenaikan biomassa kalus.ABSTRACT"

"Rattan is a spiny climbing palm that grows into the canopy of the tropical rain forest using a climbing whip in the form of cirrus or flagella. The natural distribution of rattan is from Africa, India, Sri Lanka, China, Malay Peninsula, Indonesian Archipelago, Papua New Guinea until Australia and Fiji. There are 9 genera and about 300 species of rattans in the Indonesian Archipelago.In the forest of Indonesia, rattan grows from the lowland until the mountain area, that is from 0 to 2,900 meters about sea level (m asl). Its habitat is mostly on most area with annual rainfall above 2,000 to 4,000 mm per year.Almost all part of rattan canes are used by people surrounding forest area for many of their everyday life. For Indonesia, rattan is a non timber forest product that gives the greatest income to the economy of the country. The country supplies 90 % of the world demands on rattan cane as the raw material for furniture.For a management of a forest, it is believed that much basic knowledge about the nature of the forest is needed. One of them is to develop the forest as a resource of cane industry in a sustainable way. For this purpose the composition, distribution and density of rattan species in Gunung Halimun National Park (TNGH) were studied as a model. To facilitate a familiarity to the identity of rattan in TNGH, a study on the rattan flora of Java have been conducted. Hence the purpose of the study is to provide a manuscript of a field guide book of the rattan flora of Java and a study of the species composition, density and distribution of rattan in TNGH.Data were collected from December 1994 until May 1995. For the rattan Flora of Java, all specimens herbarium at Herbarium Bogoriense and Forest Research and Development Center and Nature Conservation were observed. Quantitative characters were noted and measured to create the identification key and description of the species. For species composition on rattan in TNGH, three areas were observed namely in Gunung (G.) Kencana, G. Pameungpeuk, and G. Pangkulahan using a continues square transect method, from elevation 800 - 1,400 m asl.The result of the study shows that, there are five genera consisting of 24 species of rattan in Java: Korthalsia (two species), Ceratolobus (two species), Plectocomia (two species), Calamus (14 species) and Daemonorops (four species). It is found that, Ceratolobus glaucescens, C. pseudoconcolor and Plectocomia longistigma are not included in the previous study done by Backer and Bakhuizen van den Brink, Jr.(1968), however this study supports their opinion that Calamus spectabilis and Daemonorops palembanicus are not found in Java.From three areas in TNGH, it was found that there are 13 species of rattans in the region. In terms of species richness and densities, G. Pameungpeuk comes first, follows by G. Pangkulahan and G. Kencana. Calamus heteroideus, C. javensis, Daemonorops melanochaetes, and Plectocomia elongata are dominant both in seedling and nature forms. The rattans are relatively abundant in the areas less than 1000 m asl. and decrease in number of species as well as the minimal population in the higher altitude. Calanms ornatus occures in 800 - 1,400 m asl., Daemonorops ruber in 800 - 1,500 m asl., D. oblongus in 800 - 1,400 m asl . According to previous studies by DransfieId (1974) and Mahyar (1983), they were found only from 0 - 800 m asl."

"Telah dilakukan penelitian terhadap kayu Syzygium aqueum Syzygium cumini dan Syzygium jambos di FMIPA UI kampus Depok Penelitian ini bertujuan untuk mengetahui kualitas serat kayu Syzigium tersebut sebagai kayu substitusi bahan baku kertas Kualitas serat kayu diketahui dengan cara mengukur dimensi serat dan menghitung nilai turunan dimensi serat Preparat maserasi kayu dibuat untuk pengukuran dimensi serat Kemudian nilai turunan dimensi serat dihitung berdasarkan data pengukuran dimensi serat Hasil pengukuran dimensi serat dan penghitungan nilai turunan dimensi serat dibandingkan dengan tabel standar kriteria Hasil yang didapatkan dari penelitian ini adalah ketiga serat kayu Syzygium tersebut masuk ke dalam kelas III Karakteristik serat kelas III yaitu serat pendek dan dinding serat relatif tebal

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Research about wood fiber of Syzygium aqueum Syzygium cumini and Syzygium jambos in FMIPA UI Depok had been conducted This research is aimed for knowing the wood fiber quality of those three Syzygium wood as substitute wood for paper pulp making raw material Wood fiber quality can be analyzed by measuring fiber dimensions and calculate the fiber dimensions derived values Wood maseration slide preparations were made for measuring fiber dimensions Fiber dimension derivate value were calculated based on the measuring data of fiber dimensions The resulting data of fiber dimensions and their derived values were compared with the table of criteria standard The result shows that those three Syzygium wood are grouped in class III the fibers are short and have thick fiber wall "

"Hutan mangrove didefinisikan sebagai tipe hutan dengan kekhasannya, pada umumnya tumbuh di sepanjang pantai atau muara sungai dan hanya dapat tumbuh di daerah beriklim tropis dan/atau subtropis. Lingkungan tempat tumbuh vegetasi mangrove dipengaruhi oleh pasang surut air laut, salinitas, topografi dan sifat fisika kimia tanah.Pada tahun 80 an kawasan mangrove di Kabupaten Bangkalan mengalami alihfungsi yang berlebihan. Kegiatan alih fungsi tersebut pada akhirnya mengakibatkan dampak yang cukup serius terhadap siklus kehidupan laut dan berpengaruh pula pada ekosistem darat. Dampak yang sangat terasa adalah terjadinya abrasi, hilangnya beberapa jenis biota pantai dan adanya intrusi air laut serta berkurangnya penghasilan nelayan tradisional.Untuk menanggulangi kerusakan dan punahnya kawasan mangrove di Kato. Bangkalan, khususnya di pantai Desa Tengket dan Kool, maka Pemerintah Daerah telah melakukan program reboisasi dan rehabilitasi. Pelaksanaan penghijauan yang di mulai sejak tahun 1987, dan dalam kurun waktu 10 tahun keberhasilan di Desa Tengket cukup tinggi, artinya vegetasi jenis R.mucronata dan A.marina dapat tumbuh dengan subur. Sebaliknya di Desa Kool untuk jenis R.mucronata tumbuh kerdil, sedangkan A.marina tidak tumbuh.Perbedaan keberhasilan ini di duga disebabkan oleh perbedaan kualitas substrat pendukung pertumbuhan vegetasi di kedua desa tersebut. Jika dugaan ini benar, maka hal ini merupakan masalah penelitian yang menarik untuk di teliti.Dengan mengacu pada hasil penelitian Hardjowigeno (1989) dan Aksornkoae (1993), yang disebut dengan substrat pendukung adalah (1) Kualitas sifat fisik kimia tanah, tekstur dan warna tanah, kandungan C organik tanah dan mineral-mineral lain yang diperlukan untuk pertumbuhan (N, Ca, P, K, Mg dan S); (2) Salinitas dan pH tanah; (3) Lama penggenangan yang dipengaruhi pasang surut air laut.Penelitian ini bertujuan untuk mengetahui faktor-faktor yang berpengaruh terhadap pertumbuhan vegetasi mangrove dengan sistem zonasi yang ada. Untuk selanjutnya penelitian ini dapat digunakan sebagai masukan bagi penentu kebijakan pada instansi terkait dalam pengambilan keputusan untuk kelancaran dan keberhasilan program penghijauan kawasan hutan mangrove, dengan menekan kegagalan serendah mungkin.Di kawasan penelitian Desa Tengket diperoteh hasil bahwa: tekstur tanahnya halus dengan warna tanah abu-abu kehitaman. Kandungan bahan C organik, Nitrogen dan bahan mineral Ca, K, P, Mg, dan S yang dibutuhkan vegetasi untuk pertumbuhan tergolong pada kategori sedang sampai tinggi (3 - 5%), sehingga mendukung pertumbuhan kedua jenis vegetasi yang ada.Salinitas tanah di bawah tegakan R.mucronata dan A.marina adalah 2,1 %o pada saat pasang surut dengan pH 5,1, dan 9,2%o pada saat pasang naik, pHnya mencapai 6,1. Salinitas dan pH yang ada, mendukung pertumbuhan kedua jenis vegetasi tersebut di atas.Sebaliknya kondisi kawasan penelitian Desa Kool dari hasil uji laboratorium menghasilkan bahwa: tekstur tanahnya adalah kasar dengan kandungan kalsium cukup tinggi, dan warna tanahnya adalah cokiat kemerahan. Kandungan bahan C organik, Nitrogen kurang dari 1%, dan bahan mineral K, P, Mg, dan S berkisar antara rendah sampai sangat rendah (0,2 - <0,1%). Salinitasnya mencapai 1- 1,1%o pada saat pasang surut, dan 7,2 °Ion pada saat pasang naik. Pada kawasan ini, baik tekstur, sifat fisik kimia tanah, salinitas dan pH tidak mendukung pertumbuhan vegetasi R.mucronata dan A.marina.Topografi tanah juga berpengaruh terhadap pertumbuhan vegetasi mangrove. Pada pantai Desa Kool yang mempunyai kemiringan sejajar permukaan taut terjadi kecenderungan pada saat pasang naik, airnya dapat jauh mencapai daratan, dengan kecepatan surutnya cukup tinggi. Kondisi tersebut menyebabkan lama penggenangan atau frekuensi genangannya juga berlangsung cepat. Kemiringan pantai Desa Tengket berkisar ? 1°, sehingga proses penggenangan air laut pada saat pasang naik terjadi cukup lama.Dengan kondisi substrat pendukung yang telah disebutkan di atas, maka kerapatan vegetasi yang tumbuh di kawasan pantai Desa Tengket mencapai 3228 tegakanJha. Sebaliknye vegetasi yang tumbuh di kawasan penelitian Desa Kool berkisar 911 tegakan/ha.E. Daftar Kepustakaan : 45 ( 1928 - 1999).

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Influence of the Substrate Support to the Mangrove Vegetation Growth (A Case Study : Coastal Forest in Tengket and Kool Villages, Bangkalan District, Madura- East Java)Forest of the mangrove categorized as a typical forest with its uniqueness, generally grows at the coastal or at the estuary, and only able to grow in the tropical and or sub-tropical climate area. The place of the mangrove forest vegetation influenced by tidal, salinity, topography and physical and chemical properties of the soil it self.

As it could be found at other places in Indonesia, mangrove area in Bangkalan district has been extreemly changed. At last, that activity leads to a serious impact to the ecosystem of marinaI and terrestrial. Abrasion, loss of several coastal biotic species, seawater intrusion and finally revenue decrease of the traditional fishermen are impact of the mangrove deforesting area.

To avoid deterioration loss of that mangrove area, Regional Government organize Reboisation programme and Rehabilitation of the mangrove area surrounding northern coastal of Tengket and Kool villages. Rehabilitation programme has been performed for 10 years, starting in 1987, resulting difference yield from that two above villages.

Vegetations of R mucronafa and A marina grows well in Tengket village, while in Kool village, vegetation of R mucronata grows bad even A marina can not be grew at all.

This difference might be caused by the quality different of the substrate support of the vegetation on that two villages. Should this suggestion correct, it would be interesting resesarch to be realized.

Rfer to the result of Hardjowigeno (1989) and Aksornkoeae (1993) research the meaning of the supporting substrate is (1) Quality of the soil, chemicaly and phisically, textures and the colour, C organic content, and other minerals required for vegetation, i,e, N, Ca, P, K, Mg and S; (2) Salinity and pH of the soil; (3) Duration time of inundation caused by tidal.

The purpose of this research is to find such factors influencing the growth of mangrove vegetation with existing zonation. Furthermore this research result can be used as an input to the policy maker in the related institution to take a decision for the successfull of the reboisation programme of mangrove forest area by minimizing failness.

Research are of Tengket village resulting that its soil textures was smooth ,colouring greys to blackish. C Organic contents, Nitrogen and other ninerals such as Ca, K, P, Mg and S required for vegetation categorized as enough up to high (3 - 5%), so supporting both vegetation.

Soil salinity and its pH influence vegetation grow living on top off it. Rhizophora mucronata grows well at salinity between 2,1%o at pH 6,1 while salinity soil for Avicennia marina higher than 9,2 %o at pH 6,5 (inundation) and 5,5 (dry). Rhizophora mucronata grows well at high salinity, while Avicennia marina grows at fluctuated salinity.

But research are of Kool village resulting that its soil textures was sandy with high calsium content, soil colouring is brown to radish. C Organic contents, Nitrogen for less than 1% and other ninerals such as K, P, Mg and S required for vegetation categorized as between low up to very low (0,2 - < 0,1%). Soil salinity in Kool village 1-1,1%o and pH 7,6 (dry condition) and salinity reach 7,2%o and pH 8,8 if inundation take place. Support substrate in Kool village not support R.mucronata and A.marlna to grow well.

Based on the laboratory analysis, conclusion could be taken thar Rhizophora mucronata species used in the reboisation programme at Tengket village coastal are agreewith kind of soils and its textures and other conditionrequired for growing. Hence Rhizophora mucronata vegetation can be grow well, and become 15 - 20 meters high within 10 years. For the Kool village control area, reboisation with Rhizophora mucronata could not grow well, since texture and its kind of soil on that area were corally with thin layer mud. With the same reboisation duration, the vegetation only reach 1 - 1,5 meter high. Due to the kind of soil in Kool village only Rhizophora stylosa vegetation could be grew well.

Based on condition of the supporting substrate as described above, density of the vegetation growth on the shore of Tengket village reaching 3228 vegetation / ha. On the contrary, vegetation growths on the shore of Kool village reach about 911 vegetation/ha.

E. Bibliography: 45 (1928 - 1999)"

"Penelitian ini bertujuan untuk mencari tanaman potensial yang dapat dijadikan sebagai bionutrien. Tanaman potensial yang dipilih pada penelitian ini adalah tanaman JPR. Pada penelitian ini, terlebih dahulu dilakukan analisis awal kandungan N, P dan K yang terdapat dalam tanaman JPR. Untuk mengetahui kondisi optimum terhadap nitrogen yag terekstrak, dilakukan tahapan optimasi yaitu optimasi konsentrasi ekstraktan, optimasi waktu ekstraksi serta optimasi massa tanaman JPR. Kemudian, bionutrien JPR diaplikasikan terhadap tanaman cabai merah keriting untuk mengetahui pengaruhnya terhadap tanaman cabai merah keriting. Untuk mengetahui pengaruh yang diberikan oleh bionutrien JPR, dosis yang digunakan bervariasi. Dosis yang digunakan antara lain 15 mL/L, 25 mL/L, 50 mL/L, 75 mL/L, 100 mL/L dan 150 mL/L. Hasil yang diperoleh pada analisis awal kandungan yang terdapat pada tanaman JPR antara lain, kandungan nitrogen sebesar 117 mg/L, kandungan fosfor sebesar 11,52 mg/L, dan kandungan kalium sebesar 104,955 mg/L. Kondisi optimum pada proses ekstraksi tanaman JPR dilakukan pada konsentrasi ekstraktan 0,5 M, waktu ekstraksi selama 90 menit serta jumlah massa tanaman JPR sebesar 70 gram dengan nitrogen yang terekstrak sebesar 1053 mg/L. Dosis optimum yang diperoleh berdasarkan penelitian yang dilakukan terjadi pada dosis 150 mL/L yang memiliki kosntanta laju pertumbuhan tanaman cabai merah keriting sebesar 0,112 hari-1, sedangkan tanaman kontrol memiliki konstanta laju pertumbuhan sebesar 0,121 hari-1. Pemanenan buah cabai terbesar ditunjukkan oleh tanaman dengan dosis 100 mL/L dengan buah sebanyak 143 buah dan massa buah sebesar 638 gram, pada tanaman kontrol jumlah buah yang dipanen sebanyak 269 buah dengan massa buah sebesar 1106,3 gram."

"ABSTRAKPengaruh eksudat akar dan ekstrak daun Helianlhus mmuus serta Ipomoea batala' terhadap pertumbuhan rumput gajah Petmiselum po/ystacltyon telah dilakulrnn; diamati pula pertumbuhannya bila ditanam di dalam 1 pot bersama H. annuus atau l batatas.Pada penelitian ini. eksudat akar H. mvruus mampu menurunkan persentase perkecambahan biji dan panjang kecambah rumput gajah sampai 23,87 dan 47 % terhadap kontrol dalam 90 jam; sementara eksudat akar I. halalas menunjukkan penurunan 22,37% dan 23,83 %.Eksudat akar dari tanaman bunga matahari yang disiramkan selama 5 minggu pada rumput gajah umur 10 hari menekan tinggi gulma itu 13,62%; berat segar dan berat kerlng 39,56 dan 51,24 %. Eksudat akar tanarnan ubi jalar hanya berpengaruh menekan berat segardan berat kerlng gulma itu sampai 18,58 dan 18,40%.Ekstrak daun H. anmms sertal. hatatas 4 % b.k. mampu mengbambat persentase perl:ecambahan dan tinggi kecambah rumput gajah dalam 90 jam berturut-turut sebesar 38,45 dan 15,28% serta 30,79 dan 19,45%.Ekstrak daun H. annuus sertal. halalas 2% b.k.yang disiramkan sekali seminggu Rumput gajah yang ditanam dalam 1 pot bersama H. annuus atau l halatas tidak menunjukkan perbedaan nyata dalam tinggi dan berat segar rumput gajah; namun berat kering menunjukkan penurunan 33,44 dan 39,63% terhadap kontrol.

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ABSTRACT

Pennisetum polystachyon is native of Tropical Africa. It has a high reproductive capacity and rapid seed germinationit becomes a troublesome weed when it takes over waste- and cultivated lands. It is now also found along the road sides and highways in Indonesia. The seeds are wind dispersed and have a resilient ability to survive drought and certain cultural and chemical control methods.

The concept that some crop plants may be allelopathic to certain weeds is receiving increased attention in the search for alternative weed control strategies. Helianthus ammus and Ipomoea aquatica are amongst the crop plants that may have the allelopathic effect to some weeds.

This research aims to study the inhibiting potential of H. mmuus and I. batatas on the growth of P. polystachyon. This study observed the effects of root exudates and leaf extrects of H. annuus and /. batatas on the germination and growth of P. polystachyon; and also on the growth of this weed grown together with H. a1muus or I. batatas.

Root exudates taken from the sand planted with H. mmuus or I. halalaS for 4 Root exudates liquid comes out from watering H. ammus or/. batatas (01, 2, 3 and 4 plants/pot), poured over a 10-days seedling of P. polyslachyon twice a day during 5 weeks (5 replications each) reduced the height, fresh and dry weight of the weed. The height reduced up to 13.62 %; fresh and dry weight up to 39.56% and 51.24% by the root exudate of H. annuus; while those of L batatas had no effect to the height of P. polystachyon hut did reduce the fresh and dry weight up to18.58 and 18.40%.

Leaf extracts of H. anmms or I. halalas (0--4 % dry weight), with 5 replications each, reduced also the percentage of germination and length of the germination of seed ofP. polystachyon. During 90 hours, leaf extracts of H. ammus reduced the percentage of gennination of the weed species up to 38.45 % and length of the germination of seeds up to 15.28 %; while those of L halalas up to 30.79% and 19.45% respectively.

The I0 days-old seedling of P. polystachyon with SO mlleaf extracts of H. anmtus or L halalas (0; 0.5; J.O; 1.5; and 2% dry weight) once a week, showed a little difference effect on those weed growth a week after the third treatment. Leaf extract of H. anmms almost had no effect on the weed growth both in height, fresh and dry H. annuus- P. polystachyon and /. batatas- P.polystachyon grown together in a pot (0-5; 1-4; 2-3; 3-2; 4-1; and 5-0) for 5 weeks; 4 replications each, gave another result; both had no effect on height and fresh weight of P. polystachyon. H. annuus­ P. po/ystachyon and I. halalas- P. polystachyon 4-1 reduced the dry weight significantly up to 33.44 and 39.63 %."

"Phalaenopsis celebensis is a rare and endemic species of Sulawesi. In this experiment flower stalk culture was chosen as a method to propagate the species, since generative propagation has not been given satisfied results. Othe advantage is that this method does not need to risk the live of the rare and preciuous cllection. Phalaenopsis celebensis was survived in MS medium supplemented with 5 ppm BA (Benzyladenine). Nodal cuttings of P. celebensis flower stalk under 20-25 C exhibit three growth pattern, either developed into shoots, flower stalk or remain dormant."

"Latar belakang: Buah Merah (Pandanus conoideus Lam.) merupakan salah satu kekayaan biodiversitas alam Indonesia khas Papua yang sejak lama dikonsumsi sebagai makanan dan dikenal memiliki khasiat obat antara lain terhadap penyakit keganasan. Namun disisi lain, dasar ilmiah cara pengobatan tersebut belum diketahui secara pasti sehingga perlu dilakukan penelitian bagi pembenaran ilmiah pemakaian tanaman obat tersebut. Minyak Buah Merah diketahui mengandung beta karoten dan tokoferol yang tinggi. Berbagai penelitian menunjukkan tokoferol dapat menghambat pertumbuhan kanker payudara manusia pada kultur melalui induksi berhentinya sintesis DNA, diferensiasi sal, dan apoptosis. Karotenoid menurunkan pertumbuhan tumor payudara, meningkatkan ekspresi gen proapoptosis p53 dan BAX, menurunkan ekspresi gen antiapoptosis Bcl-2, dan meningkatkan rasio BAX:Bcl-2 pada tumor. Gen .repressor p53 dapat menginduksi cell cycle arrest sehingga memungkinkan terjadinya perbaikan DNA dan apoptosis.Tujuan: mengetahui pengaruh pemberian MBM per oral sebanyak 0,5ml, Iml, dan 2ml terhadap aktivitas apoptosis dan pertumbuhan tumor kelenjar susu mencit C3H.Rancangan penelitian: merupakan penelitian in vivo, digunakan 4 kelompok, 3 kelompok uji dan 1 kelompok kontrol, masing-masing 10 ulangan. Aktivitas apoptosis dilihat melalui indeks apoptosis menggunakan uji TUNEL. Perturnbuhan tumor dinilai dengan melihat aktivitas proliferasi menggunakan pulasan histokimia AgNOR. Selanjutnya dicari kemaknaan antara indeks apoptosis dan nilai AgNOR dart kelompok kontrol dan kelompok uji.Hasil dan Kesimpulan: Analisis varian menunjukkan tidak ada perbedaan bermakna antara indeks volume, berat, dan indeks apoptosis kelompok kontrol dan kelompok uji (p>0.05) walaupun ada kecnderungan adanya peningkatan aktivitas apoptosis pada mencit yang medapatkan MBM. Sebaliknya terdapat perbedaan yang bermakna (p<0,05) antara nilai AgNOR kelompok kontrol dan kelompok uji. Dan hasil uji multiple comparison untuk menetapkan dosis optimal dart ketiga dosis (0,5m1, 1 ml, 2m1), didapatkan bahwa dosis 2m1 terbukti bermakna secara statistik menurunkan proliferasi. Dengan demikian pada penelitian ini dapat disimpulkan adanya efek penekanan aktivitas proliferasi pada mencit yang mendppatkan MBM per oral dengan dosis optimal 2m1.

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Background: Red Papua (RP) is one of the Indonesian natural properties. Originally, RP comes from Papua province. It is consumed by Papua people as daily food since a long time ago. RP is considered to be cancer medicine, particularly for breast cancer treatment. Alternatively, the justification on the use of RP as an anti cancer has not been established yet. Hence, scientific evidence on anticancer effect of RP is necessary. The previous study suggests that Red Papua Oil (RPO) contains a huge amount of carotene and of tocopherol. Some studies indicate that tocopherol can inhibit the growth of human breast cancer cell culture by prohibiting of DNA synthesis, induction of cell differentiation and apoptosis. Carotene reduces the rate of growth of breast tumor, improves gene expression of pro-apoptosis and p53 of BAX, degrades gene expression of anti-apoptosis Bcl-2, and increases the ratio of BAX: BCL-2. P53 gene can induce arrest cycle cell so that it enables cells to repair the DNA and generate apoptosis.Aims: to identify the influence of RPO intake of 0,5m1, 1m1, and 2ml doses per oral on apoptosis and its effect on tumor growth in mammary gland tumor of C3H strain mouse.Method: Twenty-four mice were randomly divided into four groups, three groups of RPO-treated groups and one control group. After tumor was transplanted, the three doses of RPO were given to the three groups. By twenty-eight day treatment, apoptosis and proliferation activities were measured_ TUNEL essay was used to detect apoptotic activity_ The tumor growth, which was determined by proliferation activity, was assessed by a histochemical technique, AgNOR (silver-staining nucleolar organizer regions). Statistical significance of Apoptosis Index (AI) and AgNOR value in control and groups treated with varied doses of RPO were calculatedResults and Conclusion: Variant analysis indicated that there was no significant difference of volume, weight and AI among control group and treated groups (p>0.05). However, there was a tendency to an increased apoptotic activity on the treated groups. On the contrary, AgNOR values were significantly different (p"