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Herlina Asnawi
"Telah dilakukan penelitian untuk mempelajari pengaruh
iradiasi sinar gamma pada karakteristika kimia dan fisika vitamin D, minyak jagung dan campuran keduanya dengan dosis radiasi 0,10,20 dan 30 kGy dan penyimpanan 0,1,2 dan 3 bulan. Penyimpanan untuk minyak jagung dilaksanakan pada suhu kamar (29 ± 2 0C), sedang untuk vitamin D dan campurannya disimpan ditempat yang sejuk (± 15°C).
Parameter yang diuji untuk minyak jagung meliputi bilangan asam, bilangan penyabunan, bilangan iod, bobot jenis, indeks bias, metil ester asam lemak dengan kromatografi Cairan-gas, kadar vitamin D . dengan spektrofotometer, titik lebur vitamin D dengan " Differential Thermal Analyzer " kestabilan vitamin D dan campurannya dengan kromatograf I cairan- cairan tekanan tinggi.
Hasil pengujian menunjukkan bahwa bilangan iod, bobot
jenis, indeks bias dan metil ester asazu lemak dan minyak jagung tidak dipengaruhi.dosis radiasi dan penyimpanan. Iradiasi sampai 30 kGy memberikan pengaruh terhadap bilangan asam minyak jagung dan menurunkan kestabilan camnpuran. Penyimpanan member ikan pengaruh terhadap b ilangan penyabunan dan bilangan asam minyak jagung. Titik lebur, kadar dan kestabilan vitamin D menurun karena iradiasi sampai 30kGy dan penyimpanan saiapai 3 bulan.

Studies on effect of irradiation by gamma rays on
chemical and physical characteristics of vitamine D, corn
oil and their mixture with radiation dose 0,10,20.and 30 kGy
respectively and storage for 0,1,2 and 3 months have been
done. Corn oil was stored at room temperature (29 ± 2°C)
while vitamine D and the mixture were in a cool place
(.± 15°C).
The parameter studied were acid value, saponification
value, iod value, 'density, refraction index, fatty acid metil
ester of corn oil with Gas Liquid Chromatography; vitamine
•D assays with Spectrophotometre; melting 'point of vitamine
D with Differential Thermal Analyzer where stability
of vitamine D and the mixture by High Pressure Liquid Chromatography.
The results obtained, didn't show any influence of
radiation dose and storage periode towards iod value, density,
refraction index and fatty acid meti1 ester of corn oil.
Irradiation dose up to 30 kGy affected the acid value of
corn oil and decreased the mixture stability, while storage
periode changed saponification and acid value of corn oil.
Irradiation dose up to 30 kGy and storage until 3 months reduced
melting point, stability and concentration of vitamine
D.
"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1985
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
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Maria Sasi Febriaty
"Telah dilakukan penelit'ian kemungkinan penggunaan
teknjk sterilisasj radiasi untuk vitamin A palmitat,minyak
zaitun dan campuran keduanya dengan cara mempela -
jar pengaruhiradiasi sinar gamma pada karakteristika
kimia dan fisika vitamin A palmitat, rninyak zaitun, dan
campuran keduanya dengan dosis radiasi 0, 10, 20,30 kGy
dan penyimpanan 0, 1, 2, 3 bulan. Parameter yang diuji
untuk minyak zaitun ialah kelarutan , bilangan asam,bilangan
penyabunan, bilangan iod, bobot jenis, indeka bi
as dan kestabilan 'metil asam lemak bebas dengan kroma -
tografi cairan-gas . Untuk vitamin A palmitat dilaku -
kan uji kestabilan kadar dengan kromatografi cairan
cairan
Hasil pengujian menunjukkan bahwa kadar vitamin
A palmitat , bilangan asam , bilangan penyabunan,bh1an
an lad dipengaruhi oleh dosis radiasi 10, 20, 30 kGy
Penyimpanan selama 1, 2, 3 bulan mempengaru.hi kadar vitamin
A palmitat, bilangan asam, bilangan penyabunan
bilangan iod dan indeks bias minyak zaitun ( p)o,05 )

The posibility of using radiosteri]4zation technic
for vitamine A palmitate, olive oil and mixture of both
by studying the effect of Gamma rays in chemical and
physical characteristics of vitamine A palmitate, olive
oil and their mixture with radiation dose ( 0, 10 , 20
30 ky ) 'and storage ( 0 9 1, 2, 3 months ). The parameter
tested for olive oil were solubility , acid - saponifica
tion - iod values , density, refraction index and stabi
lity of free fatty acid metil ester with Gas Liquid Chro
matography . Vitamine A palmitate assays was determined
using High Pressure Liquid Chromatography.
The results obtained suôh that the concentration
of vitamine palmitate , acid - saponification-jod values
weie affected by iradiation dose . (. 10, 20 , 30 kGy ).
Storage for. 1, 2 and 3 months influence the concentration
of vitamine A palmitate , acid-saponificatinn-iod values
and refraction index of olive oil. ( p )0.05 )
"
Depok: Universitas Indonesia, 1985
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UI - Skripsi Membership  Universitas Indonesia Library
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Fajar Lukitowati
"ABSTRAK
Penelitian ini menganalisis pengaruh iradiasi sinar gamma terhadap sifat-sifat fisiko-kimia dan biologi membran kitosan, kolagen, dan paduan kitosan/kolagen. Membran kitosan, kolagen, dan kitosan/kolagen dibuat dengan penguapan pelarut dan membran diiradiasi sinar gamma (0, 15 atau 25 kGy). Pengujian untuk mengamati gugus fungsi, kuat tarik, perpanjangan putus, daya serap air, permeabilitas, sterilitas serta daya tembus mikroba. Data diuji statistik. Terdapat perubahan gugus fungsi, penurunan kuat tarik, perpanjangan putus, daya serap air dan permeabilitas membran serta kenaikan sterilitas pada membran tanpa dan dengan iradiasi, kecuali untuk daya tembus mikroba. Iradiasi sinar gamma pada semua membran menimbulkan perubahan sifat fisiko-kimia dan sterilitas

ABSTRACT
The objectives of this study is to analyze the effects of gamma-ray irradiation to physico-chemical and biological properties chitosan, collagen and blend of chitosan/collagen membranes. The solvent evaporation technique is used to prepare chitosan, collagen and chitosan/collagen membranes, and sterilized by gamma-ray irradiation (with dose of 0, 15 or 25 kGy). Functional groups, mechanical strength, water retention, permeability, sterility and microbial penetration are observed. The data was analyze statistically. Functional groups, tensile strength, elongation at breaks, water retention, permeability, and sterility are changes, except for microbial penetration. Gamma-ray irradiation on chitosan, collagen and blend of chitosan/collagen membranes shows changes of physico-chemical and sterility."
2016
T46396
UI - Tesis Membership  Universitas Indonesia Library
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Hadi Suprayitno
Depok: Fakultas Teknik Universitas Indonesia, 2001
S49153
UI - Skripsi Membership  Universitas Indonesia Library
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Susanto
"Iradiasi gamma banyak digunakan oleh beberapa industri obat herbal sebagai metode pengawetan yang efisien dalam mengurangi kontaminasi mikroorganisme. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh iradiasi gamma (0; 5; 7.5; and 10 kGy) pada ekstrak etanol H. sabdariffa (EEHS) terhadap uji total mikroorganisme, total kandungan fenol dan flavonoid, aktivitas antioksidan, TLC profiling, total kuersetin dan bobot molekul, aktivitas penghambatan terhadap enzim α-glukosidase dan in-vitro analisis terhadap galur sel kanker manusia (A-549,HUT-78, dan MCF-7). Iradiasi dosis 5 kGy menunjukkan bahwa tidak adanya kapang yang tumbuh dan terjadi penurunan jumlah total bakteri, lebih lanjut iradiasi pada dosis 10 kGy tidak terjadi pertumbuhan bakteri. Analisis total fenol, dan flavonoid, serta aktivitas antioksidan menunjukkan adanya penurunan sebesar 5-11% setelah diiradiasi pada dosis 5 kGy. Analisis profil TLC dan HPLC menunjukkan bahwa salah satu senyawa dalam EEHS adalah kuersetin yang ditunjukan dengan adanya [M+H]+ pada m/z 303,04 dari hasil analisis LC-Ms/Ms. EEHS juga memiliki penghambatan terhadap aktivitas enzim α-glukosidase dengan nilai penghambatan 4,75-7,55%. Uji aktivitas anti kanker terhadap galur sel kanker manusia menunjukkan bahwa EEHS memiliki kemampuan menginhibisi sel kanker sangat kuat dengan nilai IC50 < 20 µg/mL. Lebih lanjut, khasiat anti kanker paling kuat terhadap HUT-78, dengan nilai IC50 10,51 µg/mL, diikuti terhadap MCF-7 (IC50 13,39 µg/mL), dan  A-549 (IC50 14,19 µg/mL). Diketahui pula bahwa iradiasi dosis 5-10 kGy mampu menurunkan aktivitas anti kanker, namun penurunan tersebut tidak menghilangkan aktivitasnya yang ditandai nilai IC50 < 20 µg/mL. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa iradiasi gamma dapat digunakan sebagai pengawetan pada ekstrak etanol H. sabdariffa Linn.

Gamma irradiation is widely used by many herbal medicine industries as an efficient preservative method in reducing microorganism contamination. The purpose of this study was to evaluate the effect of gamma irradiation (0; 5; 7.5; and 10 kGy) on the ethanol extract of H. sabdariffa (EEHS) toward the total microorganism test, total phenol and flavonoid contents, antioxidant activity, TLC profile, total quercetin and its molecular weight, inhibitory activity against α-glucosidase enzyme , as well as in-vitro bioassay against human cancer cell lines (A-549, HUT-78, and MCF-7). Irradiation at a dose of 5 kGy showed that no mold grew and there was a decrease in the total number of bacteria, moreover at a dose of 10 kGy there was no bacterial growth. Analysis of total phenols and flavonoids, as well as antioxidant activitiy showed a decrease of 5-11% after irradiation at a dose of 5 kGy. TLC and HPLC profile analysis showed that one of the compounds in the H. sabdariffa extract was quercetin which was indicated by the presence of [M+H]+ at m/z = 303.04 from the LC-MS/MS analysis. EEHS also had inhibitoty  activity against α-glucosidase enzyme with the inhibition value of 4.75-7.55%. Bioassay anticancer against human cancer cell lines showed that EEHS had a very strong ability to inhibit cancer cells with the IC50 value < 21 µg/mL. Furthermore, the anti-cancer properties were strongest against HUT-78 with the IC50 value of 10.51 µg/mL, followed by MCF-7 (IC50 13.39 µg/mL), and A-549 (IC50 14.19 µg/mL). It is also known that irradiation doses of 5-10 kGy could reduce anti-cancer activity, however the decrease did not eliminate its activity which was the IC50 values still lower than 20 µg/mL. Based on the data obtained, it can be concluded that gamma irradiation can be used as a preservative method for ethanol extract of H. sabdariffa Linn."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023
T-pdf
UI - Tesis Membership  Universitas Indonesia Library
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Muhammad Imam Surya
"ABSTRAK
Sweet sorghum is a kind of sorghum that contains high content of sugar in its stem. Sweet sorghum has a big potential to be developed in Indonesia owing to its wide adaptation and the fact that it can be used as raw material for liquid sugar, syrup, ethanol, and also as animal feed. Sweet sorghum has not been developed in Indonesia because of lack of a sweet sorghum variety. Improvement of available sweet sorghum genotype can be done among others through plant breeding program. First step on the plant breeding program is to increase the plant genetic variability. This might be done by introduction of varieties or by breeding to create new varieties. Induced mutation using Gamma irradiation can be used to increase the genetic variability of sweet sorghum. Mutation breeding using Gamma irradiation in sweet sorghum was aimed at improving the yield and quality of sweet sorghum.
This research was conducted to study the effect of Gamma irradiation on sweet sorghum growth in the M1 generation, and to estimate the optimal dose range suitably for the breeding program. Beside, the objective of this research was to evaluate the genetic variability for the purpose of plant selection in the M2 generation.
Plant materials consisted of 2 sweet sorghum lines introduced from ICRISAT namely line No. 79 and No. 83. Non-saccharin sorghum of local variety Fiigari was used as a control. The doses of Gamma irradiation treatment were 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 Gy. The Ml plants were sown in greenhouse at PATIR-BATAN Jakarta, and then were transplanted in the experimental field at Balitbiogen, Bogor. The M2 plants were grown in the experimental field at Lubang Buaya, Jakarta. Important agronomic traits such as plant height, spike length, stem diameter, and grain weight/spike were observed.
The results indicated that sorghum lines gave different response to Gamma irradiation, and all measured variables were significantly affected. Irradiation gave morphology and physiology damages on sorghum like abnormality, sterility, and lethality in the Ml generation. The increase of irradiation doses increased physiological damage. Effective doses of Gamma irradiation for sweet sorghum was to be around 400-500 Gy, and the lethal doses 50% of sweet sorghum was around 800-1000 Gy. Putative mutation sometimes could be observed in the M2 generation. The treatment of Gamma increased genetic variability of plant height, spike length, stem diameter, and grain weight/spike. The highest genetic variability was found in the dose treatment of 200-300 Gy. Within this interval dose, there might be high probability to find desirable mutants for further breeding purpose. A number of 38 plants had been selected from the M2 population as putative mutants.
"
2007
T20182
UI - Tesis Membership  Universitas Indonesia Library
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I Wayan Balik Sudarsana
"Telah dilakukan pengukuran untuk menentukan OF berkas lapangan terbuka dan berkas filter wedge ternyata hasilnya tidak beda. Mengukur dosis primer tidak bisa dilakukan secara eksperimen melainkan secara teori, dengan cara ekstrapolasi kurva OF pada kedalaman 0,5 cm diperoleh sebesar 0,7066. Nilai PDD hasil pengukuran dari 0,5 cm sampai 20 cm tidak jauh berbeda dengan nilai yang berikan oleh BJR. Perbedaan keduanya berada dalam rentang -3,36% sampai 0,60%. Radiasi primer untuk kedalaman rendah ditentukan dengan pendekatan hubungan antara dosis relatif dengan luas lapangan sedangkan untuk kedalaman lebih tinggi dari 3 cm pendekatan dosis relative sebagai fungsi linier Z. Nilai PDD radiasi primer dibandingkan dengan nilai yang diberikan BJR dari 0,5 cm sampai 20 cm perbedaan dalam rentang -4,31% sampai 9,28%.

A measurement has been performed to know the output factors for open and with wedge filters beams the result indicate the same value . Dose primary can?t measurement but just calculate and than primary OF from OF curve ekstrapolation for 0.5 cm deep are 0.7066. PDD value measurement for 0.5 cm to 20 cm there were not so difference value with PDD BJR. Both different are - 3.36% to 0.60%. Primary dose for less than 3 cm solution from dose relative linier with field size. If more than 3 cm solution from dose relative with Z. PDD primary dose compare with BJR for 0.5 cm to 20 cm are -4,31% to9,28%."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2007
T21052
UI - Tesis Membership  Universitas Indonesia Library
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