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"Efek pemberian ekstrak etanol biji klabet (Trigonella foenum-graecum L.) terhadap pengurangan kerapuhan tulang pada tikus (Rattus norvegicus) betina galur Sprague-Dawley telah diteliti. Dua puluh empat ekor tikus usia 3 bulan dibagi dalam 6 kelompok, terdiri dari kelompok kontrol normal, kelompok ovariektomi (ovx), kelompok ovx-tamoksifen 1,8 mg/kg BB, serta 3 kelompok perlakuan ovx yang masing-masing diberi ekstrak etanol biji klabet dosis150 mg/kg BB, 300 mg/kg BB, dan 600 mg/kg BB. Bahan uji diberikan selama 12 minggu berturut-turut. Hasil uji ANAVA (P < 0,05) terhadap kadar ALP dan kalsium serum menunjukkan tidak ada pengaruh yang nyata pemberian ekstrak etanol biji klabet pada ketiga kelompok dosis. Hasil uji ANAVA(P < 0,05) menunjukkan terdapat pengaruh yang nyata pemberian ekstrak etanol biji klabet terhadap peningkatan rerata ketebalan dan derajat densitas trabekula pada ketiga kelompok dosis.

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The effect of the ethanolic extract of seed of Trigonella foenum-graecum L. toward the bone loss in ovariectomized (ovx) rats were investigated. Twenty-two- 3-months-old female Sprague-Dawley were randomly assigned to six groups as followed, 3 control groups and 3 treatment groups. The control groups consist of the normal group, the ovx group, and the ovx group treating with tamoxifen 1,8 mg/kg BB. The treatment groups consist of the ovx group treating with ethanol extract of seeds of Trigonella foenum-graecum L. dose of 150 mg/kg BB, 300 mg/kg BB, and 600 mg/kg BB. The treatment done every day for 12 weeks. The ANAVA test (P > 0,05) resulted no real effect of treatment toward the mean of level ALP and calcium serum to those treatment groups. The Anava test (P>0.05) resulted the real effect of treatment toward the mean of thickness and density of cancellous bone to those treatment groups. The thickness and density of cancellous bone of treatment groups increased toward normal group."

"Buah Leunca (Solanum nigrum L.) adalah salah satu tanaman obat Indonesia yang dikenal memiliki aktivitas sebagai antidisentri, antiinflamasi, dan fitoestrogen. Kandungan kimia buah leunca antara lain diosgenin, solanin, solamargin, dan chaconine. Penelitian ini dilakukan untuk mengetahui efek pemberian ekstrak etanol buah leunca (Solanum nigrum L.) terhadap pengurangan kerapuhan tulang pada tikus betina galur Sprague-Dawley. Tiga puluh ekor tikus dibagi dalam 6 kelompok terdiri atas kelompok kontrol normal, kelompok ovariektomi (OVX), kelompok OVX-tamoxifen dosis 1,8 mg/kg bb, dan 3 kelompok OVX-ekstrak etanol buah leunca dosis 150 mg/kg bb, 300 mg/kg bb, dan 600 mg/kg bb. Bahan uji diberikan selama 12 minggu berturut-turut. Parameter yang diamati adalah kadar kalsium dan alkalin fosfatase (ALP) darah serta histologi tulang femur.Hasil penelitian menunjukkan bahwa mulai dosis 300 mg/kg bb terjadi peningkatan densitas dan ketebalan trabekula tulang femur yang bermakna (α < 0,05) bila dibandingkan kontrol ovariektomi dan setara dengan kontrol normal. Kesimpulan dari penelitian ini adalah pemberian ekstrak etanol buah leunca memiliki potensi mengurangi kerapuhan tulang.

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Black Nightshade fruit (Solanum nigrum L.) is one of Indonesian medicinal plant which known showing many activities such as antidisentry, antiinflamation, and also as phytoestrogen. Black Nightshade fruit contains diosgenin, solanine, solamargine, and chaconine. This research was conduct to investigate the effect of ethanolic extract of black nightshade fruit (Solanum nigrum L.) on ovariectomized rats bone loss. Thirty-3-months-old female rats Sprague-Dawley strain were randomly divided into six groups, namely 3 control groups and 3 treatment groups. The control groups consist of normal group, ovariectomized (ovx) group, and ovx group treating with tamoxifen 1,8 mg/kg bb. The treatment groups consist of the ovx group treating with ethanolic extract of black nightshade fruit dose of 150 mg/kg bb, 300 mg/kg bb, and 600 mg/kg bb. The treatment done every day for 12 weeks.The result showed that start on 300 mg/kg bb, ethanolic extract of black nightshade fruit increased significantly (α < 0,05) the density and thickness of trabecular of femur bone. We can conclude that ethanolic extract of black nightshade fruit has potentially effect to decrease bone loss."

"Phytoestrogen is the chemical compound contains in plant which has estrogenic like effect. Estrogen has important function on woman?s sexual organ, such as proliferation of uterine and vaginal cornification. Phytoestrogen are weak agonists for estrogen and illicit statistic significantly increases in uterine wet weight, atdefinite dose, in uterothrophic bioassay. Biji Klabet or Fenugreek seed (Trigonella foenum-graecum L.) contains steroidal sapogenins such as diosgenin, tigogenin, gitogenin, yamogenin and trigoneoside, that suspected having an estrogen-like effect or as phytoestrogen. The aim of this research was to investigate the estrogeniceffect of fenugreek?s ethanolic extract in ovariectomized and immature rats models. These models represent the limacteric/menopause phase, where estrogen level is very low because ovary produces no estrogen. The testing animals were divided into normal group, ovariectomized control group, estradiol control group and three leveldoses of fenugreek extract (30mg/200gBW; 60mg/200gBW and 120mg/200gBW). The result indicated that start on 60mg/200gBW, fenugreek extract significantly could increasing mammary gland proliferation. Empirically fenugreek containing diosgenin, that caused breast enhancement. This research showed that treatmentwith fenugreek extract can caused proliferation of mammary gland, both on immature and ovariectomized rats."

"Kerontokan rambut bisa disebabkan banyak faktor antara lain kekurangan hormon estrogen. Penambahan estrogen secara eksogen diduga dapat mengubah siklus hormonal yang dalam beberapa kasus dapat memicu timbulnya kanker. Salah satu alternatif rasionil adalah dengan menggunakan senyawa mirip estrogen hasil isolasi dari tanaman yang disebut fitoestrogen. Fitoestrogen dapat berkompetisi dengan estrogen untuk berikatan dengan reseptornya sehingga timbul efek estrogenik. Senyawa fitoestrogen pada biji klabet diduga dapat meningkatkan proses pertumbuhan rambut. Tujuan penelitian ini adalah untuk membuktikan efektivitas sediaan tonik rambut ekstrak biji klabet pada proses pertumbuhan rambut kelinci, mendapatkan konsentrasi optimal dan data sensitivitasnya. Aktivitas pertumbuhan rambut ditentukan melalui perhitungan panjang rambut, diameter rambut dan berat rambut. Uji sensitivitas dinilai dengan Draize skin test dan Draize eye test. Hasil uji aktivitas tonik rambut yang mengandung ekstrak biji klabet 10%, menunjukkan perbedaan bermakna (p < 0,05) dibandingkan dengan plasebo dan menyerupai pemberian tonik rambut yang mengandung minoksidil 2%. Hasil uji sensitivitas menunjukkan efek iritasi ringan.

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There are many causes of hair loss, among others is estrogen deficiency. External estrogen administration could change the hormonal cycle and increased cancer risk. One of the natural alternative estrogen therapy can be found in various plants containing natural product among which are compounds with weak estrogenic activity, termed phytoestrogens. Phytoestrogens compete with estrogen by filling or binding to the estrogen receptor and producing the estrogens effect. Phytoestrogene in fenugreek seeds (Trigonella foenum-graecum L.) is believed to increase hair growing process; however, up to now there is no scientific study to prove it.

Therefore, the objection of this study is to prove the effect of hair tonic containing fenugreeks seeds extract in different concentration on hair growing activity of New Zealand strain rabbit ; and to get the optimal concentrations of fenugreek extract as well as the safety data. Hair growing activity is determined by hair length, hair diameter and hair weight measurement, while toxicity test is determined by Draize skin test and Draize eye test.

The result of the activity test using 10% fenugreek extract seed hair tonic showed significant difference (p < 0,05) compare to placebo and resemble the result using minoxidil 2% hair tonic. Sensitivity test results showed mild irritation effects."

"Penelitian dilakukan untuk mengevaluasi pengaruh penggunaan kombinasi etilen glikol (EG) sebagai krioprotektan intraseluler dan kuning telur sebagai krioprotektan ekstraseluler dengan variasi konsentrasi dalam mempertahankan jumlah dan morfologi folikel preantral ovarium tikus setelah vitrifikasi selama 48 jam. 20 Sprague-Dawley betina berumur 12 minggu digunakan sebagai hewan model penelitian. Folikel yang diuji merupakan folikel tahap preantral yang berasal dari ovarium kanan saja. Sampel ovarium utuh (n = 20) dibagi ke dalam 7 kelompok yaitu KK, KKP 1, KKP 2, KKP 3, KP 1, KP 2, dan KP 3.Kelompok kontrol (KK) merupakan ovarium segar tanpa vitrifikasi. Kelompok kontrol perlakuan (KKP 1, KKP 2, dan KKP 3) merupakan ovarium yang diberi perlakuan vitrifikasi dalam etilen glikol (EG) dengan konsentrasi berturut-turut 3,75%; 7,5%; dan 15%. Kelompok perlakuan (KP 1, KP 2, dan KP 3) merupakan ovarium yang diberi perlakuan vitrifikasi dalam kombinasi EG dan kuning telur (1 : 1) dengan konsentrasi masing-masing berturut-turut 3,75%; 7,5%; dan 15%. Setelah diisolasi dari tikus, ovarium KK segera difiksasi sedangkan ovarium KKP dan KP dibekukan dalam nitrogen cair (−196 ° C). Setelah 48 jam, sampel ovarium dicairkan dan difiksasi.Seluruh kelompok ovarium difiksasi selama 48 jam. Masing-masing ovarium lalu dibuat sayatan histologis dengan ketebalan 5 um, kemudian diwarnai dengan pewarna hematoksilin eosin (HE). Folikel preantral ovarium diamati keutuhan morfologinya dan dihitung jumlahnya. Data hasil pengamatan menunjukkan bahwa berdasarkan uji Kruskall-Wallis (P < 0,05) tidak terdapat perbedaan yang signifikan pada jumlah folikel preantral ovarium 48 jam pascavitrifikasi. Berdasarkan hal tersebut, dapat disimpulkan bahwa vitrifikasi menggunakan kombinasi etilen glikol (EG) dan kuning telur tidak berpengaruh dalam mempertahankan folikel preantral ovarium pascavitrifikasi.

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The study was conducted to evaluate the effect of combination of ethylene glycol (EG) as intracellular cryoprotectant and egg yolk as extracellular cryoprotectant with varied concentrations in maintaining morphology and counts of ovarian pre-antral follicles after vitrification for 48 hours. Twenty 12 week old Sprague-Dawley female rats were used as animal models. The follicles tested were preantral stage follicles from right ovary only. Whole ovary samples (n = 20) were divided into 7 groups, namely NC; TCG 1; TCG 2; TCG 3; TG 1; TG 2; and TG 3.

The control group (NC) consists of fresh ovaries without vitrification. The treatment control group (TCG 1; TCG 2; and TCG 3) consists of ovaries treated with vitrification in ethylene glycol (EG) with a concentration of 3.75%; 7.5%; and 15%, respectively. The treatment group (TG 1; TG 2; and TG 3) were ovaries treated with vitrification in a combination of EG and egg yolk (ratio 1: 1) with a concentration of 3.75%; 7.5%; and 15%, respectively. After being isolated from the rats, the NC ovaries were immediately fixated while the TCG and TG ovaries were frozen in liquid nitrogen (−196 °C). After 48 hours, the ovary sample is thawed and fixated.

All ovarian groups were fixated for 48 hours. Each of the ovaries was then made a histological incision with a thickness of 5 µm, then stained with a hematoxylin eosin (HE). Ovarian pre-antral follicles were observed for the integrity of the structure and counted. The data based on Kruskall-Wallis test (P < 0.05) show that there were no significant differences in the number of ovarian preantral follicles 48 hours after vitrification. It can be concluded that vitrification using a combination of ethylene glycol (EG) and egg yolk has no effect in maintaining ovarian preantral follicles after vitrification."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian madu PS (Pollen substitute) terhadap penurunan konsentrasi kolesterol total plasma darah tikus putih jantan. Dua puluh empat ekor tikus putih jantan dibagi dalam 4 kelompok, yaitu kelompok kontrol normal (KK1) yang hanya diberi akuades, kelompok kontrol perlakuan (KK2) yang diberi diet lemak tinggi dan akuades, dan 2 kelompok perlakuan (KP1 dan KP2) yang diberi diet lemak tinggi, Propylthiouracil (PTU) dan madu PS dengan konsentrasi berturut-turut 10% dan 20%. Bahan uji diberikan setiap hari selama 14 hari. Pengambilan darah dilakukan pada hari ke-0 (T0) dan hari ke-14 (T14). Analisis konsentrasi kolesterol total dilakukan dengan metode Cholesterol Oxidase Para Aminophenazone (CHOD-PAP). Hasil uji anova satu faktor (P<0,05) pada akhir penelitan menunjukkan adanya pengaruh nyata pemberian madu PS terhadap konsentrasi kolesterol total pada semua kelompok perlakuan. Hasil uji LSD (P<0,05) menunjukkan penurunan kolesterol total pada seluruh kelompok perlakuan (KP1 dan KP2). Penurunan konsentrasi kolesterol total terbesar dicapai oleh kelompok perlakuan konsentrasi 20% (KP2) dengan persentase penurunan mencapai 16,22% dibandingkan terhadap KK2. Berdasarkan hasil tersebut dapat disimpulkan bahwa pemberian madu PS dapat menurunkan konsentrasi kolesterol total secara signifikan.

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The study was undertaken to assess the effect of pollen substitute (PS) honey on blood total cholesterol concentration of Sprague Dawley rats (Rattus norvegicus L.). Twenty four rats were divided into four treatment groups, consisting of a normal control group (KK1) which had no high lipid diet treatment; a treatment control group (KK2); which was fed with a high lipid diet only; and two treatment groups (KP1 and KP2) which were fed .with a high lipid diet, Propylthiouracil (PTU), and PS honey at different concentrations, 10% and 20% respectively, for 14 consecutive days. The treatment was carried out by gavage method. The concentrations of blood total cholesterol were analyzed with Cholesterol Oxidase Para Aminophenazone (CHOD-PAP) method.

One way anova test (P<0,05) showed that blood total cholesterol concentrations decreased significantly. LSD test (P<0,05) showed that the decreased blood total cholesterol concentration occurred at both treatment groups (KP1 and KP2). The concentration of blood total cholesterol reduced into the normal range. Even though the reducing effect of PS honey was showed at both doses of 10% and 20%, the more prominent result was gained by the later one. It is concluded that feeding with PS honey significantly lowered blood total cholesterol concentration."

"Keringnya kulit menua pada wanita disebabkan oleh penipisan struktur kulit, berkurangnya produksi Natural Moisturizing Factor serta penurunan kadar estrogen. Krim pelembab kulit menua pada umumnya mengandung gliserin dalam konsentrasi tinggi, tetapi kurang disukai ketika digunakan. Tujuan penelitian ini adalah menggabungkan 4% ekstrak etanol biji kelabet dan 2% malam lebah untuk menghasilkan krim pelembab (krim uji) yang lebih efektif, aman, disukai serta stabil untuk melembabkan kulit menua dibandingkan dengan gliserin 10% (krim kontrol). Uji manfaat (efektivitas) kedua krim diukur berdasarkan pengamatan skor gambaran klinis dan skor korneometer CM825® pada 33 sukarelawan wanita berusia 30-45 tahun. Uji keamanan dinilai dengan patch test. Tingkat kesukaan kedua krim dinilai dengan uji perbandingan jamak. Uji stabilitas mencakup cycling test, uji mekanik, uji stabilitas dipercepat selama 12 minggu serta uji efektivitas pengawet. Hasil uji manfaat menunjukkan krim uji dinilai lebih efektif untuk melembabkan kulit menua dibandingkan dengan krim kontrol. Hasil uji keamanan menunjukkan kedua krim tidak mengakibatkan iritasi. Uji perbandingan jamak menunjukkan kedua krim samasama kurang disukai. Tidak terjadi pemisahan fase pada uji stabilitas dan penggunaan pengawet dinilai sudah cukup efektif. Berdasarkan penelitian, terdapat perubahan warna, pembesaran diameter globul, penurunan kadar pH, penurunan viskositas serta konsistensi, sehingga dapat disimpulkan kedua krim yang dihasilkan kurang stabil.

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Dry aging skin on women is caused by thinning of skin structure, less production of Natural Moisturizing Factor and estrogen level decrease. Generally, moisturizer creams for aging skin contain high concentrate of glycerin, although less comfortable to use. The aim of this research is to combine 4% ethanol extract of fenugreek seeds and 2% of beeswax to produce moisturizer cream (herbal cream) which is more effective, safe, comfortable and stable to moisturize the aging skin compared to glycerin 10% (control cream). Efficacy test for both creams were measured based on observation of clinical score and corneometer CM825® score for 33 women volunteers aged 30-45 years old. Safety test was evaluated by patch test. The comfortability level for both creams were determined by pair comparison test. The stability test consists of cycling test, mechanic test, accelerated stability test within 12 weeks and preservative effectiveness test. The result of efficacy test showed that the herbal cream was more effective to moisturize the aging skin compared to the control cream. The safety test indicated that both creams did not cause irritations. The pair comparison test showed that both creams were less comfortable to use. There was no emulsion breaking during stability test and preservative was adequately effective. During the research, there were change in color, enlargement of globule diameter, decrease in pH level as well as viscosity decrease and inconsistency; hence it can be concluded that both creams were less stable."

"Telah dilakukan penelitian untuk mengetahui bahwa ekstrak etanol rimpang temu mangga Curcuma mangga Val. berpengaruh terhadap kadar bilirubin total dan bilirubin direct akibat kerusakan hati yang diinduksi oleh karbon tetraklorida CCl4 . Hewan uji yang digunakan dalam penelitian yaitu 24 ekor tikus Rattus norvegicus L. jantan galur Sprague-Dawley yang dibagi menjadi enam perlakuan KK1, KK2, KP1, KP2, KP3, dan KP4 dengan empat kali ulangan. Kelompok KK1 merupakan kelompok kontrol normal yang tidak diinjeksikan CCl4 dan dicekok CMC 0.5 , KK2 merupakan kelompok kontrol perlakuan yang diinjeksikan CCl4 sebanyak 1 ml/kgBB secara intraperitoneal dan dicekok CMC 0,5 . Kelompok KP1, KP2, KP3, dan KP4 merupakan kelompok perlakuan yang diinjeksikan CCl4 1 ml/kgBB dan diberikan ekstrak temu mangga dengan dosis berturut-turut 10 mg/kgBB, 20 mg/kgBB, 40 mg/kgBB, dan 80 mg/kgBB. Hasil uji non parametrik Kruskal-Wallis ? = 0,05 menunjukkan bahwa dosis 10 mg/kgBB, 20 mg/kgBB, 40 mg/kgBB, dan 80 mg/kgBB berpengaruh terhadap kadar bilirubin total dan bilirubin direct. Hasil uji perbandingan berganda Dunnett T3 ? = 0,05 menunjukkan bahwa dosis-dosis tersebut tidak berbeda bermakna dengan KK1. Dengan demikian dosis-dosis tersebut memiliki efek kuratif karena dapat menurunkan kadar bilirubin total dan direct sampai mendekati kadar normal.

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This study was conducted in order to observe that the ethanol extract of mango ginger rhizome Curcuma mangga Val. affect the level of total bilirubin and direct bilirubin due to liver damage induced by tetrachloride carbon CCl4 . The test animals in the study were 24 male rats Rattus norvegicus L. of Sprague Dawley strain that was divided into six treatment KK1, KK2, KP1, KP2, KP3 and KP4 and repeated four times. The KK1 group is a normal control group that was not injected with CCl4 and 0.5 CMC fed, KK2 group is a treatment group that was intraperitoneally injected with CCl4 treatment in the amount of 1 ml kgBW and 0.5 CMC fed. KP1, KP2, KP3 and KP4 are treatment groups that got injected with CCl4 1 ml kgBB and were given mango ginger rhizome ethanol extract each with a dose of 10 mg kgBW, 20 mg kgBW, 40 mg kgBW, and 80 mg kgBW respectively by oral. The results of Kruskal Wallis non parametric test 0,05 shows that the dose of 10 mg kgBW, 20 mg kgBW, 40 mg kgBW, and 80 mg kgBW impacted on total bilirubin and direct bilirubin levels. Dunnet rsquo s T3 0,05 multiple comparison test result shows that the dosages had no significant differences with KK1 group. In conclusions, the dosages could be deemed as have curative effects since they successfully reduce the level of total bilirubin and direct bilirubin until it approached normal level."

"Penderita karatak di Indonesia setiap tahun semakin meningkat. Usia penderita katarak juga semakin muda. Penyembuhan dengan operasi mahal dan beresiko gagal. Oleh karena itu, dibutuhkan pengobatan dengan sediaan obat yang mudah dibuat dan aman digunakan. Bunga telang (Clitoria ternatea L.) mengandung antosianin yang memiliki kemampuan untuk meluruhkan katarak. Ekstraksi air panas terhadap 2 tangkai bunga telang pada 20 ml aquades menghasilkan kadar antosianin maksimum pada suhu pelarut 80oC yaitu 2,5 mg/l. Katarak diuji pada hewan coba tikus (Rattus norvegicus) galur Sprague Dawley jantan berumur 10 hari hasil induksi natrium selenit (Na2Se2O3) 20 μmol/kg BB tikus. Pengujian kemampuan peluruhan katarak dilakukan dengan cara meneteskan ekstrak bunga telang pada mata tikus. Penetesan dilakukan dengan dosis 2, 3, dan 4 tangkai bunga telang dan frekuensi penetesan 1x,2x dan 3x sehari. Sifat keaktifan peluruhan katarak diuji dengan melihat penurunan tingkat kekeruhan lensa mata tikus dari tingkat 5 hingga 1. Hasil penetesan menunjukkan semakin besar dosis antosianin dan semakin sering dilakukan penetesan memberikan peluruhan katarak yang semakin besar. Penetesan ekstrak bunga telang dengan dosis 2 tangkai dan frekuensi penetesan 3x sehari menghasilkan tingkat kekeruhan terendah yaitu tingkat 1. Dosis 4 tangkai dan frekuensi penetesan 1x sehari menghasilkan tingkat kekeruhan tertinggi yaitu tingkat 4. Kemampuan peluruhan ekstrak bunga telang berkisar antara 20% pada dosis 4 tangkai dan 1x penetesan hingga 80% pada dosis 2 tangkai dan 3x penetesan.

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The number of cataract patients in Indonesia keeps increasing every year. It has also affected younger people. Healing with operation is getting more expensive and has higher risk. Hence, it needs more alternative medicine which can be easily made and found. Butterfly pea (Clitoria ternatea L.) contains anthocyanin which is able to decay the cataract. The Extract of the butterfly pea with 80oC water, which resulting the level of the extracted anthocyanin is 2.5 mg/l. The cataract, induced from sodium selenite (Na2Se2O3) 20 μmol/kg is tested on male ten-days-old laboratory rats, Rattus norvegicus.

The experiment of cataract decaying is done by shedding the pea?s extract on the rats? eyes. The shedding is practiced with 2, 3, and 4 stalks of butterfly peas, and the frequency is once, twice and third times a day. The characteristics of the cataract decaying are experimented by observing the turbidity level reduction of the rats eyes, with five to one scales.

The shedding shows the higher dose of anthocynin and more often the frequency, the bigger cataract will be decayed. The extract shedding of two stalks of butterfly peas and the third times a day frequency produces the lowest turbidity level which is one. The extract of four stalks of butterfly peas and once a day frequency ends with the highest turbidity level which is four. The decay ability of the extract butterfly pea is around 20% at dose 4 stalks and once frequency and 80% at dose 2 stalks and third times frequency of shedding."

"Telah dilakukan penelitian untuk mengetahui pengaruh pemberian madu PS (Pollen Substitute) terhadap konsentrasi trigliserida plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Dua puluh empat tikus jantan dibagi dalam empat kelompok, yaitu kelompok kontrol normal (KK1) yang tidak diberi diet tinggi lemak, kelompok kontrol perlakuan (KK2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan akuades, dan dua kelompok perlakuan (KP1 dan KP2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan madu PS dengan dosis masing-masing 10% dan 20% b/v. Bahan uji diberikan setiap hari selama 14 hari berturut-turut. Hasil uji anava satu faktor (P < 0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap konsentrasi trigliserida akhir pada semua kelompok perlakuan. Hasil uji LSD (P<0,05) menunjukkan penurunan konsentrasi trigliserida pada seluruh kelompok perlakuan (KP1 dan KP2). Konsentrasi trigliserida pada kelompok perlakuan yang mendekati kelompok kontrol normal dicapai oleh kelompok perlakuan dengan dosis 20% b/v.

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The research was done to observe the effect of honey PS (Pollen Substitute) on the concentration of triglyceride in blood plasma of male rat (Rattus norvegicus L.) Sprague-Dawley strain. Twenty-five male rats were divided into four groups, consisting of normal control group (KK1) that was not given a high fat diet, treatment control group (KK2) that was given a high fat diet, 0.01% propylthiouracil (PTU) solution, and two treatment groups (KP1 and KP2) that were given a high fat diet, 0.01% propylthiouracil (PTU) solution, and honey PS with dose of 10% and 20% w/v, respectively, for 14 consecutive days.

The result of anova test (P < 0.05) showed significant effect of honey PS toward triglyceride concentration in all treatment groups. LSD test (P < 0,05) showed that the decreased blood total cholesterol concentration occurred at both treatment groups (KP1 and KP2). The concentration of triglyceride in the treatment group that approached the normal control group achieved by the treatment group with dose of 20% w/v."