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"Fifteen rhizopus oligosporus isolated were isolated from a number of tempeh samples obtained from Mataramm Jember an d Bogor Indonesia;...."

"The relationships among allium L. species remain complex. Certain species belonging to the genus allium are medically and economically important and as such merit phylogenetic analysis...."

"Sebagian besar bakteri asam laktat (BAL) menghasilkan eksopolisakarida (biopolimer fruktan) yang mempunyai banyak manfaat dalam industri makanan, kosmetik, kesehatan dan farmasi. Sintesis biopolimer ini melibatkan peran enzim fruktansukrase atau fruktosiltransferase (ftf). Rekayasa genetika dapat dilakukan untuk mendapatkan biopolimer yang berkriteria unggul, yaitu biopolimer inulin yang mempunyai derajat polimerisasi tinggi. Weissella confusa galur MBFCNC-2(1) telah menjadi sumber gen fruktansukrase yang dikloning lengkap di inang E. coli BL21 StarTM.Tujuan penelitian ini adalah untuk mendapatkan klon versi terpenggal dari gen fruktansukrase karena klon gen lengkap dilaporkan mempunyai masalah dalam ekspresinya. Sebagai template untuk kloning digunakan plasmid dari E. coli BL21 StarTM rekombinan, plasmid rekombinan pO_ftfNS pembawa gen lengkap, dan DNA genomik. PCR dilakukan menggunakan primer FTFdel_Fw dan FTFdel_Rv. Hasil PCR disekuensing dan dianalisis menggunakan BLAST. Sebagai hasil, gen fruktansukrase versi terpenggal didapatkan dari plasmid rekombinan pO_ftfNS pembawa gen ftf lengkap.

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Most of Lactic Acid Bacteria (LAB) produce exopolysaccharide (fructan biopolymer) that has many advantages in food, cosmetic, health, and pharmacy industries. Synthesis of this biopolymer involves the role of fructansucrase enzyme of fructosyltransferase (ftf). Genetic engineering could be done to obtain biopolymer with excellence characteristcs, that is inulin with high degree of polymerization. Weisella confusa strain MBFCNC-2(1) has been used as a source of fructansucrase gene which is full-length clonned at E. coli BL21 StarTM.The aim of this study was to obtain truncated gene of fructansucrase because fulllength clone has problem on its expression. The PCR template used in this study were plasmid of Recombinant E coli BL21 StarTM, recombinant plasmid pO_ftfNS carrying full-length gene, and genomic DNA. PCR was carried out by FTFdel_Fw and FTFdel_Rv primer. The PCR product was sequenced and analyzed by using BLAST. Result revealed that truncated fructansucrase gene was obtained from plasmid recombinant pO_ftfNS carrying full-length gene."

"ABSTRACTActin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotidesequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region ofArabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to acting from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis."