Hasil Pencarian  ::  Simpan CSV :: Kembali

"This book reports on CXCR4 antagonists, an exciting new class of compounds that aid in use of stem cells for treatment of leukemia and other cancers. Includes coverage of plerixafor, now approved for stem cell mobilization of lymphoma and myeloma patients."

"Advances in stem cell research discusses recent advances in stem cell science, including therapeutic applications. This volume covers such topics as biomanufacturing iPS cells for therapeutic applications, techniques for controlling stem cell fate decisions, as well as current basic research in such areas as germ line stem cells, genomics and proteomics in stem cell research. "

"This volume aims to cover important aspects of the various facets of organ transplantation and regenerative medicine."

"In recent years, cancer stem cells have been recognized as important component in carcinogenesis and they seem to form the basis of many (if not all) tumor types. Cancer stem cells or "cancer cell like stem cells" have been isolated from various cancers of different origin (blood, breast, brain, skin, head and neck, thyroid, cervix, lung, retina, colon, pancreas and so on). Cancer stem cells - rare cells with indefinite proliferative potential that drive the formation and growth of tumours- seem to show intriguing relationships with physiological stem cells. Specifically, these cancer cells show significant similarities in the mechanisms that regulate self-renewal of normal stem cells. Moreover, tumour cells might directly arise from normal stem cells. Further, the cellular biology of cancer stem cells show a lot of similarities with normal stem cells."

"This text explores the latest progress in cell transformation model systems, and explores molecular and cellular changes at work in the transformation of normal cells into neoplastic cells. Also covers stem cells, and the role of microenvironments in cancer."

"Latar belakang: Sel Punca Mesenkimal (SPM) berfungsi sebagai penyedia komponen osteogenik dalam penyembuhan fraktur. Pada kasus dengan defek tulang, penyembuhan juga memerlukan komponen osteokonduktif (scaffold). Kalsium hidroksiapatit sulfat (HA-CaSO4) telah digunakan secara luas sebagai bone void filler, mampu berperan sebagai scaffold untuk SPM, namun belum ada penelitian yang mengevaluasi pengaruh scaffold terhadap viabilitas SPM. Metode: Dilakukan isolasi SPM dari sumsum tulang krista iliaka kelinci Giant Flamish dan diekspansi dalam medium DMEM rendah glukosa dengan teknik histogradient density. Setelah 1 minggu, sel di subkultur pada TC flask ukuran 25cc sebagai pasase pertama, kemudian dilakukan pergantian medium setiap 3 hari. Ketika subkultur dikerjakan, kami membenamkan pellet HA-CaSO4 pada flask tersebut. Lalu evaluasi dilakukan pada sel tersebut dengan menggunakan mikroskop cahaya setiap minggu. Hasil: Pada minggu pertama, SPM sangat sulit diidentifikasi karena dominasi kristal HA-CaSO4. Memasuki minggu ketiga, SPM telah tumbuh dan kristal HA-CaSO4 sudah dibilas dengan pergantian medium. Pada minggu keempat, SPM tetap terlihat pada evaluasi. Kesimpulan: HA-CaSO4 dapat digunakan sebagai kandidat scaffold yang unggul untuk menghantarkan SPM secara in vivo tanpa mempengaruhi kelangsungan hidup SPM.

---

AbstractBackground: Many studies have reported the role of Mesenchymal Stem Cells (MSC) in treating fractures. In case with bone defect, fracture healing needs not only osteogenic but also osteoconductive component (scaffold). Hydroxyapatite calcium sulphate (HA-CaSO4) being widely used as bone void filler, may serve as scaffold for MSC. However, the effect of this scaffold to the viability of MSC has not been evaluated before. Methods: MSC were isolated from the iliac marrow of a Giant Flamish rabbit, and expanded in DMEM using histogradient density. After one week, they were sub-cultured in a 25cc TC flask (passage 1) and have the medium replaced every 3 days. During the subculture, we embedded a HA-CaSO4 pellet into the flask. The cells were evaluated under inverted microscope at a weekly interval. Results: At the first week, MSC are difficult to be identified in microscope due to the large number of HA-CaSO4 crystals. By the third week however MSC have grown and the HA-CaSO4 crystals can readily be washed off by medium replacement. By the fourth weeks, MSC can be still seen on microscope. Conclusion: HA-CaSO4 could serve as a good scaffold due to its pellet shape and easily absorbed, thus providing revascularization which is essential for bone healing.In addition, HA-CaSO4 does not interfere with MSC survival."