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"Latar Belakang: PRF adalah trombosit generasi kedua yang digunakan untuk mempercepat penyembuhan jaringan lunak dan keras dan merupakan matriks fibrin alami yang mengandung platelet dan leukosit sitokin. Pemberian growth Factor yang cukup lama (TGF β1) dari penambahan PRF pada DPBB dapat mengakibatkan peningkatan regenerasi tulang dibandingkan dengan pemberian DPBB saja. Tujuan: mengevaluasi pengaruh platelet rich fibrin dalam meningkatkan regenerasi tulang yang dilihat melalui konsentrasi TGF β1 dan osteocalcin. Metode: autologous PRF dibuat dengan menggunakan 10 ml darah yang dikumpulkan dari regio antecubital dari relawan. Sampel darah diperlakukan sesuai dengan protokol PRF (Dohan et all.2006). Bekuan fibrin di tengah tabung digunakan sebagai tambahan untuk bahan bone graft (DPBB). Kelompok-kelompok ini sampel PRF diinkubasi pada suhu 370C dan 5% CO2 selama 1 hari, 5 hari dan 7 hari. Semua sampel dipanen pada waktu yang tepat dan disimpan lalu diperiksa Enzyme-Linked Immunosorbent Assay (ELISA) analisis untuk TGF β1 tingkat dan tingkat ostecalcin. Hasil: terdapat perbedaan bermakna dari PRF + DPBB dibandingkan dengan DPBB di hari 1, dari PRF dibandingkan dengan kontrol pada hari 5, dari PRF + DPBB dibandingkan dengan kontrol pada hari ke 7, dan dari DPBB dibandingkan dengan kontrol pada hari ke 7 (p <. 05). Terdapat perbedaan bermakna dari PRF + DPBB dibandingkan dengan kontrol pada hari ke 1,5 dan 7, dari DPBB dibandingkan dengan kontrol pada hari 1,5, dan 7, dan dari PRF membandingkan toPRF + DPBB dan DPBB (p <.05). Kesimpulan: Penambahan PRF atau PRF + DPBB dalam kultur osteoblas dapat meningkatkan konsentrasi TGF β1 dan osteokalsin, yang dimulai 1 hari ke hari 7

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Background: PRF is a second generation platelet used to accelerate soft and hard tissue healing and is a strictctly autologous fibrin matrix containing a large quantitiy of platelet and leukocyte cytokines. Given prolonged growth factor (TGF β1) from PRF treatment in DPBB might result in increasing bone regeneration compare to those treated with DPBB. Purpose: evaluate the influence of platelet rich fibrin in enhancing natural bone regeneration through the levels of TGF β1 and osteocalcin. Method: Autologous PRF was prepared by using 10 ml of autologous whole blood collected from the antecubital regio of a volunteers. Blood samples were treated according to the PRF protocol (Dohan et all.2006). A fibrin clot in the middle of tube was used as an adjunct to grafting material (DPBB). These groups of PRF samples were incubated at 370C and 5%CO2 for 1 day, 5 days and 7 days. All sample were harvested at the appropriate time and and stored for later Enzyme-Linked ImmunoSorbent Assay (ELISA) analysis for TGF β1 level and osteocalcin level. Result: There are significantly differents from PRF+DPBB compare to DPBB at day 1, from PRF compare to control at days 5, from PRF+DPBB compare to control at day 7, and from DPBB compare to control at days 7 (p<.05). There are significantly differents from PRF+DPBB compare to control at day 1,5 and 7, from DPBB compare to control at days 1,5,and 7, and from PRF compare toPRF+DPBB and DPBB (p<.05). Conclusion: Addition PRF or PRF + DPBB in cultured osteoblasts can increase the concentration of TGF β1 and osteocalcin, which began 1 day to day 7"

"[ABSTRAKLatar Belakang: Penggunaan biomaterial berupa bahan tandur tulang dan membran untuk prosedur Guided Bone Regeneration (GBR) sangat diperlukan di bidang bedah maksilofasial dan, untuk mengatasi defek tulang yang dapat terjadi oleh berbagai sebab. Penelitian ini bertujuan untuk mengetahui efek pemakaian bahan tandur tulang DFDBX dengan membran perikardium (MPK) bovine pada defek tulang kalvaria tikus.Bahan dan Metode: Studi eksperimental ini menggunakan 45 ekor tikus Sprague Dawley sebagai hewan coba dibagi dalam 3 kelompok secara acak. Ciritical size defect sebesar diameter 5 mm dibuat pada tulang kalvaria seluruh hewan coba. Kelompok I merupakan kelompok kontrol, tidak diberikan perlakuan dan defek dibiarkan sembuh dengan sendirinya, kelompok II yang diberi DFDBX, dan pada kelompok III defek diisi dengan DFDBX dan ditutup dengan MPK (DFDBX+MPK). Setelah 1,4 dan 8 minggu dilakukan dilakukan pengorbanan pada kelompok hewan coba, dilanjutkan dengan evaluasi secara radiologik, histopatologik untuk reaksi radang, pertumbuhan tulang dan pemeriksaan imunohistokimia dengan osteokalsin. Data dianalisis secara statistik dengan menggunakan uji ANOVA.Hasil: Penilaian radiografik diperoleh perbedaan bermakna pada rerata densitas area defek minggu ke 8 antara kelompok kontrol dengan DFDBX+MPK (p<0,001) dan antara kelompok DFDBX dengan DFDBX+MPK (p=0,03).Pertumbuhan tulang baru pada minggu ke 8 tertinggi adalah pada kelompok DFDBX+MPK dengan perbedaan bermakna dengan kelompok kontrol (p=0,016) dan dengan kelompok DFDBX nilai p=0,048. Ekspresi osteokalsin minggu ke-8 menunjukkan perbedaan bermakna antara kelompok kontrol dengan kelompok DFDBX (p<0,001) maupun dengan kelompok DFDBX+MPK (p=0,0013), namun tidak terdapat perbedaan bermakna antara kelompok DFDBX dengan kelompok MPK (p=1,000).Kesimpulan: Penggunaan DFDBX dengan kombinasi MPK terbukti secara radiologik, histopatologik dan imunohistokimia dapat meningkatkan regenerasi tulang pada defek tulang kalvaria.

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ABSTRACTBackground: Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has included bone grafting and using membrane in guided bone regeneration procedure. Using biomaterial such as bone grafting and membrane for Guided Bone Regeneration (GBR) procedures is an essential issue in maxillofacial and dental reconstruction surgery to overcome bone defects caused by various etiologies. Our study was aimed to identify the effect of using Demineralized Freeze-Dried Bone Xenograft (DFDBX) with (or without) bovine pericardium membrane (PCM) on the treatment of rats calvarial bone defects.Materials and Method: The experimental study used 45 Sprague-Dawley rats as the experimental animals, which were categorized randomly into three groups, i.e. the control group, DFDBX group, and DFDBX+PCM group. The 5-mm-critical-sized calvarial defects were created in all experimental animals. The first group was a control group, which did not receive any treatment with self-limiting defects; while subjects in the second group received DFDBX (DFDBX group) and in the third group, the defects were filled with DFDBX and PCM (DFDBX + PCM group). Animals were sacrified at the 1st, 4th, and 8th weeks following the surgery. Subsequently, an evaluation was carried out using radiological analysis, histopathological assay to observe inflammatory reaction and bone growth, as well as immunohistochemical analysis of osteocalcin. Data were analyzed statistically using ANOVA test. The specimens were embedded ini paraffin, serially cut, and stained with hematoxylin and eosin for analysis under light microscope. The inflammation reaction, new bone formation, and the rest of DFDBX and PCM were histomorphometrically evaluated. Immunohistochemical analysis of osteocalcin expression was performed.Results: Radiological analysis demonstrated a significant difference of mean bone density in the defect area at the 8th week between subjects in the control group and those in DFDBX+PCM group (p < 0.001), as well as between subjects in the DFDBX group and those in DFDBX+PCM group (p = 0.03). The highest rate of bone healing at the 8th week was found in DFDBX+PCM group, which showed significant difference compared to the control group (p=0.016) and to DFDBX group (p=0.048). There was a significant difference of osteocalcin expression between the control group and DFDBX group (p < 0.001), as well as between the control group and DFDBX + PCM group (p=0,0013). However, there was no significant difference between the DFDBX group and the DFDBX+PCM group (p = 1.000). Conclusion: Our radiological, histopahtological and immunohistochemical evaluation has demonstrated that DFDBX combined with PCM increases bone regeneration in the treatment of bone calvarial defect. ;Background :Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has been bone grafting and using membrane in guided boneregeneration procedure.The aim of this study was to analyze the effect of Demineralized Freeze Dried BoneXenograft (DFDBX) with (or without)bovine pericardium membrane (PCM) on boneregeneration, in surgically created critical-size defects in rat calvaria, radiographically,histopathologically and immunohistochemically.Material and Methods :Surgical critical-size bone defects were created in 45 animalsthat randomly divided into three groups : control group, DFDBX group, andDFDBX+PCM group. Animals were sacrified at 1, 4 and 8 weeks post surgery.Radiological analysis was done. The specimens were embedded ini paraffin, serially cut,and stained with hematoxylin and eosin for analysis under light microscope. Theinflammation reaction, new bone formation, and the rest of DFDBX and PCM werehistomorphometrically evaluated. Immunohistochemical analysis of osteocalcinexpression was performed. Result : DFDBX and DFDBX+PCM groups demonstrated superior bone healingcompared with control group. Group DFDBX+PCM showmore advanced healing at 8weeks post surgery and show the highest density radiographically as compared with theother group DFDBX and control.Immunohistochemistry revealed the presence ofosteocalcin in osteoblast and matrix extracellular and show significant differences werenoted between DFDBX and DFDBX+PCM to control groups.Conclusion : Application of DFDBX combined with bovine PCM gave the best result in bone regeneration of critical size defects in rat calvaria. , Background :Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has been bone grafting and using membrane in guided boneregeneration procedure.The aim of this study was to analyze the effect of Demineralized Freeze Dried BoneXenograft (DFDBX) with (or without)bovine pericardium membrane (PCM) on boneregeneration, in surgically created critical-size defects in rat calvaria, radiographically,histopathologically and immunohistochemically.Material and Methods :Surgical critical-size bone defects were created in 45 animalsthat randomly divided into three groups : control group, DFDBX group, andDFDBX+PCM group. Animals were sacrified at 1, 4 and 8 weeks post surgery.Radiological analysis was done. The specimens were embedded ini paraffin, serially cut,and stained with hematoxylin and eosin for analysis under light microscope. Theinflammation reaction, new bone formation, and the rest of DFDBX and PCM werehistomorphometrically evaluated. Immunohistochemical analysis of osteocalcinexpression was performed. Result : DFDBX and DFDBX+PCM groups demonstrated superior bone healingcompared with control group. Group DFDBX+PCM showmore advanced healing at 8weeks post surgery and show the highest density radiographically as compared with theother group DFDBX and control.Immunohistochemistry revealed the presence ofosteocalcin in osteoblast and matrix extracellular and show significant differences werenoted between DFDBX and DFDBX+PCM to control groups.Conclusion : Application of DFDBX combined with bovine PCM gave the best result in bone regeneration of critical size defects in rat calvaria. ]"

"Gen Osteokalsin merupakan gen kandidat terjadinya osteoporosis.. Polimorfisme pada gen tersebut menyebabkan densitas tulang menurun. Densitas tulang juga dipengaruhi oleh aktivitas fisik dan asupan makanan. Faktor-faktor tersebut untuk mendapatkan model prediksi tulang sehingga dapat dilakukan pencegahan. Dengan demikian dilakukan pengukuran densitas tulang, pemeriksaan biokimia darah serta polimorfisme gen osteokalsin digunakan enzim HindIII dengan teknik PCR-RFLP. Diperoleh rataan usia 67,21±9,1; IMT 22,14±4,08; fosfat alkalin 87,26±25; kalsium 8,9±0,82; estradiol 24,8±11,7; osteokalsin 1,75±0,83; mempunyai T-score ≤ - 2,5 dengan varian TT (64,3%) diikuti varian CC (60,6%) dan CT (50%) sehingga diperoleh model yang dapat memprediksi derajat keparahan tulang.

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The aim of the research to obtain a model that uses the genetic factors, the environment and nutrient to predict bone density and risk of osteoporotic fracture. Bone mineral density and biochemical markers were determined, as well as the C298T polymorphism status of osteocalcin gene using PCR-RFLP. The subjects had a mean age of 67.2±9.1 years. ; BMD 22.14±4.08; phosphate alkaline 87.26±25; calcium 8.9 ±0.82; estradiol 24.8±11.7; osteocalcin 1.75±0.83; the C298T polymorphic genotypes showed TT (64.3%) CC (60.6 %) and a CT (50%) determine in T-score≤ -2,5. We identified a model of age and the level osteocalcin that can predict severity of bone density."

"Latar Belakang: Saat ini dikembangkan suplemen media pertumbuhan alternatif selain fetal bovine serum (FBS), seperti human platelet lysate (hPL) dan advanced platelet-rich fibrin (A-PRF). Media pertumbuhan berbasis platelet seperti hPL dan A-PRF memiliki keunggulan dibandingkan dengan FBS karena bersifat xenofree. hPL dan A-PRF memiliki sejumlah besar growth factor yang diperlukan untuk proliferasi sel, terutama human dental pulp stem cells (hDPSCs). Tujuan: Menganalisis potensi human platelet lysate (hPL) dan Advance Platelet Rich Fibrin (A-PRF) yang merupakan suplemen media pertumbuhan xeno-free sebagai alternatif pengganti FBS. Metode: Analisis proliferasi hDPSCs menggunakan suplemen media pertumbuhan hPL 5%, A-PRF 20% dan 25% pada hari ke-1, ke-3 dan ke-5 dengan uji Flowcitometry dan MTT-Assay. Hasil: Jumlah proliferasi hDPSCs paling tinggi terdapat pada aplikasi A-PRF 25% (p<0,05) terhadap kontrol positif FBS 10% dan hPL 5%. Peningkatan jumlah ini berbeda secara signifikan antara hari ke-1 dan hari ke-3, serta hari ke-3 dan hari ke-5 (p<0,05). Tidak ada perbedaan bermakna pada aplikasi hPL 5% pada hari ke-1, ke-3 dan ke-5 (p>0,05) Kesimpulan: A-PRF  25% memiliki potensi paling tinggi dalam meningkatkan jumlah proliferasi hDPSCs dibanding dengan penggunaan A-PRF 20%, hPL 5% dan FBS 10%.

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Background: Currently alternative growth media other than fetal bovine serum (FBS) were developed such as human platelet lysate (hPL) and advanced platelet-rich fibrin (A-PRF). Human platelet lysate (hPL) and platelet rich fibrin lysate (A-PRF) containing abundant growth factor (GF) that can be use for human dental pulp stem cells (hDPSCs) proliferation. Aim: To analyse hDPSCs proliferation in three different supplement medias (hPL 5%, A-PRF 20% and A-PRF 25%) after 1, 3 and 5 days observation compare to FBS 10%. Methods: hDPSCs proliferation in three different supplement medias culture (hPL 5%, A-PRF 20% and A-PRF 25%) was analyzed using flowcitometry and MTT-Assay. Results: Compare to FBS 10% and hPL 5%, A-PRF 20% and 25% have significant proliferation of hDPSCs in day-1 (p<0,05). Significant proliferation seen in day-1 and day-3 also between day-1 and day-5 (p<0,05). There is no significant proliferation rate between hPL 5% in day-1, day-3 and day-5 (p>0,05). Conclusion: A-PRF 25% has the highest hDPSCs proliferation compare toA-PRF 20%,  hPL 5% and FBS 10%."

"Latar Belakang: TGF-β1 memiliki peran penting dalam proses diferensiasi sel punca pulpa (hDPSCs) menjadi sel odontoblast dan asam hialuronat (AH) sebagai perancah alami memiliki sifat biokompabilitas dan berperan dalam pembentukan jaringan keras gigi. Tujuan: Mengetahui potensi berbagai konsentrasi AH (10 mg/mL, 20 mg/mL, 30 mg/mL) pada media kultur (MK) hDPSCs terhadap ekspresi TGF-β1 dengan waktu observasi 7 hari dan 14 hari. Metode: Kultur hDPSCs didapatkan dari penelitian sebelumnya (persetujuan etik dilampirkan) yang merupakan passage 3 dan 4. Setelah 24 jam inkubasi, MK digantikan oleh media osteogenic. hDPSCs dipuasakan selama 24 jam. AH kemudian ditanam ke dalam 96 well kultur jaringan yang terdiri dari 5x103 sel/well. MK AH dibagi menjadi tiga konsentrasi (10 mg/ml, 20 mg/ml, dan 30 mg/ml) dan diinkubasi dalam atm 5% CO2, 37o C. Ekspresi TGF-β1 dianalisis menggunakan ELISA reader setelah inkubasi selama 7 hari dan 14 hari dan secara kualitatif dengan pewarnaan Alizarin Red. Analisis statistik menggunakan uji One-way ANOVA dan uji Post Hoc LSD (SPSS IBM 26). Hasil: Terdapat perbedaan potensi berbagai konsentrasi AH (p<0,05) terhadap ekspresi TGF-β1 hDPSCs pada observasi 7 dan 14 hari. Kelompok AH 30 mg/mL memiliki ekspresi TGF-β1 tertinggi. Pewarnaan Alizarin Red menunjukkan nodul berwarna merah semakin pekat dan banyak pada konsentrasi AH 30 mg/mL. Kesimpulan: AH berpotensi untuk meningkatkan ekspresi TGF-β1. Kelompok AH 30 mg/mL merupakan konsentrasi yang paling berpotensi dibandingkan kelompok lain pada waktu observasi 7 hari

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Background: TGF-β1 plays an important role in the process of differentiation of human dental pulp stem cell (hDPSCs) into odontoblast cells and hyaluronic acid (HA) as a natural scaffold has biocompatible properties and plays a role in the formation of dental hard tissue. Objective: To determine various concentrations potential of HA (10 mg/mL, 20 mg/mL, 30 mg/mL) as hDPSCs culture media (CM) towards TGF-β1 expression on 7 and 14 days observations. Methods: hDPSCs culture were obtained from those of previous research (ethical approval form attached) at P3 and P4. After 24 hours of incubation, CM was replaced with osteogenic media. hDPSCs undergo 24 hours of serum starvation and then implanted into 96 well tissue culture consisting of 5x103 cells/well. hDPSCs CM divided into three concentrations and incubated in 5% CO2 atm, 37o C. TGF-β1 expression was analyzed using an ELISA reader and qualitatively by Alizarin Red staining. Statistical analysis using One-way ANOVA and Post Hoc LSD test (SPSS IBM-26). Results: At 7 and 14 days, there is a statistically significant different potential of HA CM in various concentrations (p<0,05) towards expression of TGF-β1 hDPSCs. HA 30 mg/mL group have the highest TGF-β1 expression. Alizarin Red staining showed corellate results with more dense red nodules at HA 30 mg/mL group. Conclusion: HA have the potential to increase TGF-β1 expression hDPSCs. HA 30 mg/mL was the most potential concentration compare to other groups at 7 days observation."

"ABSTRAKLatar Belakang: Laki-laki menyumbang sekitar 40% kasus untuk infertilitas. Salah satu penyebab infertilitas yakni kasus azoospermia. Pada beberapa kasus azoospemia yang ditangani melalui teknologi reproduksi berbantu dengan kegagalan perolehan sperma dari testicular sperm extraction (TESE), maka Spermatogonial Stem Cells (SSCs) dapat menjadi salah satu alternatif terapi. SSCs dapat diperoleh dari isolasi dan kultur sel spermatogenik. Sejak abad ke 19, berbagai metode isolasi dan kultur sel spermatogenik mulai dikembangkan. Akan tetapi berbagai metode ini belum ada yang optimal. Oleh karena itu, dibutuhkan suatu teknik kultur untuk mengoptimalisasi proses ekspansi sel spermatogenik, dari segi faktor apoptosis.Metode: Pada penelitian ini dilakukan pemberian suplemen kultur berbeda pada medium kultur yakni FBS 10%, PRP 10%, dan PRP 10% ditambah faktor pertumbuhan (GDNF, bFGF, EGF) untuk proses kultur. Hasil kultur dilakukan identifikasi marka permukaan CD90 dan GFRA1 menggunkan flowsitometri dan dilakukan uji apoptosis. Fenomena apoptosis yang muncul diamati berdasar adanya fragmentasi pada DNA dengan metode TUNEL serta adanya peran eksekutor apoptosis yakni kaspase-3 yang teramati pada pengujian imunositokimia.Hasil Penelitian: Hasil analisis marka permukaan CD90 dan GFRA1 memiliki nilai berbeda- beda pada pemberian medium yang berbeda. Pertumbuhan sel kultur lebih baik dengan indeks apoptosis yang lebih rendah pada medium dengan pemberian PRP dan PRP ditambah faktor pertumbuhan (FBS= 25.01%, PRP = 9.99%, PRP+ GF= 2.47%). Nilai ekspresi kaspase-3 pada sel yang diberi suplemen FBS sekitar 21%, PRP 13% dan PRP + GF 7%.Kesimpulan: PRP lebih baik dibandingkan dengan FBS sebagai medium kultur sel spermatogenik, dari segi apoptosis.

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ABSTRACTBackground: Males contribute to 40% of the infertility cases over the universe. One of the causes of men infertility is azoospermia. In some cases of azoospemia which are handled through assisted reproductive technology with the failure of sperm retrieval from testicular sperm extraction (TESE), the Spermatogonial Stem Cells (SSCs) could be an alternative therapy. SSCs can be obtained from isolation and culture of spermatogenic cells. Since the 19th century, various methods of isolation and spermatogenic cell culture began to be developed. However, there are not optimal condition of this yet. Therefore, we need to optimize the spermatogenic cell expansion method, particularly in apoptotic factor.Method: In this study, the culture system were administrated by thesupplementation with 10% FBS, 10% PRP, and 10% PRP plus growth factors (GDNF, bFGF, FGF). Spermatogenic cells were identified the surface markers CD90 and GFRA1 using flowsitometry and apoptosis tests were performed. The apoptotic phenomenon was observed based on the presence of DNA fragmentation by the TUNEL method and the caspase-3 expression by immunocytochemical.Result: The result of surface marker had different value. The results showed better that cell culture growth and lower apoptotic index in the medium with PRP and PRP+ GF (FBS= 25.01%, PRP= 9.99%, PRP+ GF= 2.47%). Immuno-expression of caspase-3 in cells cultured with FBS 21%, PRP 13%, dan PRP+ GF 7 %.Conclusion: PRP was better than FBS as the spermatogenic cell culture medium based on apoptotic phenomenon."

"[ABSTRAKPenyembuhan defek tulang akibat trauma, antara lain dapat berupa trauma fisik; mekanik; kimia maupun biologik dapat dilakukan melalui terapi transplantasi tulang autogenik; allogenik; alloplastik dan xenogenik. Penggunaan material xenogenik yang paling sering digunakan dalam mempercepat penyembuhan adalah material dari bovine yang mempunyai potensi osteokonduktif sangat baik. Penelitian ini bertujuan untuk mengetahui perbedaan perilaku sel osteoblas manusia terhadap paparan bovine periosteal membrane dibandingkan dengan kontrol. Hasil penelitian ini menunjukkan bahwa Bovine periosteal menbrane produksi BATAN yang diujikan tidak menstimulasi proliferasi sel osteoblas setelah 24 jam pemaparan. Disamping itu, tidak terdapat perbedaan bermakna terhadap ekspresi fosfatase alkali dan konsentrasi osteokalsin pada sel osteoblas yang dipapar dengan bovine periosteal membrane dibandingkan dengan kontrol

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ABSTRACTHealing of bone defects due to trauma, including physical; mechanical; chemical and biological trauma can be done through autogenic; allogenic; alloplastic and xenogenic bone transplantation therapy. The most common xenogenic material that is used for bone healing acceleration is bovine material which has excellent osteoconductive potention. The aim of this study is to determine the differences in the behavior of human osteoblast cells on exposure to bovine periosteal membrane compared with controls. The results of this study indicate that bovine periosteal membrane from BATAN is not stimulate the proliferation of osteoblast after 24 hours of exposure. In addition, there is no significant difference on the expression of alkaline phosphatase and osteocalcin concentration in osteoblast cells exposed to bovine periosteal membrane compared with controls. , Healing of bone defects due to trauma, including physical; mechanical; chemical and biological trauma can be done through autogenic; allogenic; alloplastic and xenogenic bone transplantation therapy. The most common xenogenic material that is used for bone healing acceleration is bovine material which has excellent osteoconductive potention. The aim of this study is to determine the differences in the behavior of human osteoblast cells on exposure to bovine periosteal membrane compared with controls. The results of this study indicate that bovine periosteal membrane from BATAN is not stimulate the proliferation of osteoblast after 24 hours of exposure. In addition, there is no significant difference on the expression of alkaline phosphatase and osteocalcin concentration in osteoblast cells exposed to bovine periosteal membrane compared with controls. ]"

"ABSTRAKAbstrak. Inovasi terbaru untuk mendapatkan regenerasi jaringan periodontal adalah dengan bahan platelet rich fibrin (PRF) dan cangkok tulang. Penelitian ini merupakan penelitian eksperimental klinis. Tujuan: Mengevaluasi perbedaan tinggi tulang alveolar pada terapi bedah flep poket infraboni menggunakan Platelet rich fibrin dan kombinasi dengan cangkok tulang. Metode penelitian: Evaluasi radiografis periapikal sebelum dan sesudah perawatan menggunakan PRF dan kombinasi dengan cangkok tulang Hasil: secara statistik, terdapat perbedaan tinggi tulang yang bermakna pada terapi bedah poket infraboni dengan PRF dan kombinasi dengan cangkok tulang. Kesimpulan: Platelet rich fibrin dan kombinasi dengan cangkok tulang memiliki hasil yang sama pada evaluasi radiografis ketinggian tulang secara statistik

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ABSTRACTAbstract. The new innovation to enhance periodontal tissue regeneration are using PRF and bone graft material. The study was clinical experimental. Purpose:To evaluate the difference of alveolar bone heigh on periodontitis therapy using PRF and combination with bone graft.Research methods: periapical radiograph evaluation before and after periodontitis therapy using PRF compare to combination with bone graft. by assessing alveolar bone height. Results: Statistically, there were no significant difference between alveolar bone height on periodontitis therapy PRF compare to combination with bone graft. Conclusion: PRF and combination with bone graft has the same result statictically in radiographic evaluation of alveolar bone height."

"ABSTRAKTerapi regeneratif menggunakan sel punca hematopoietik (SPH) CD34+ merupakan potensi modalitas yang dapat diterapkan dalam mengatasi masalah penyakit hematologi yang sulit disembuhkan. Namun, kultur in vitro SPH saat ini belum optimal karena adanya reaksi penolakan dari penerima sel hasil kultur tersebut. Fetal bovine serum (FBS) sebagai suplemen medium yang umum digunakan dalam kultur SPH merupakan xeno-protein yang dapat memicu reaksi imun dari penerima prosedur terapi. Penelitian ini bertujuan untuk membandingkan antara penggunaan FBS dengan platelet rich plasma (PRP) yang berasal dari sumber manusia terhadap proliferasi dan kepuncaan SPH CD34+. Penelitian eksperimental dilakukan dengan mengukur beberapa parameter antara lain, perhitungan jumlah sel dengan metode eksklusi tryphan biru, kepuncaan SPH CD34+ dengan menggunakan flow cytometry, serta diferenisasi sel yang dinilai dengan pengamatan sel pada pewarnaan giemsa. Hasil penelitian menunjukkan bahwa suplementasi PRP 15% dapat meningkatkan proliferasi SPH CD34+. Analisis flow cytometry menunjukkan bahwa suplementasi PRP kurang mempertahankan kepuncaan SPH CD34+ dengan penurunan kemurnian CD34+ sebesar 31,7%; 31,7%; 21,7% pada kadar suplementasi PRP 5%, 10%, dan 15%. Gambaran sel mononuklear yang ditemukan pada pewarnaan Giemsa menunjukkan bahwa terjadi diferensiasi sel hematopoietik menjadi sel yang lebih spesifik. Oleh karena itu, dapat disimpulkan bahwa PRP 15% merupakan suplementasi yang yang terbaik dalam memicu proliferasi SPH diantara berbagai konsentrasi yang diuji dalam penelitian ini.

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ABSTRACTRegenerative therapy using CD34+ hematopoietic stem cells (HSC) is a potential modality to overcome hematological diseases that are difficult to cure. However, the current in vitro CD34+ culture is not optimal because of the immunological rejection from the recipient. Fetal bovine serum (FBS) as a medium supplement, which commonly used in HSC culture, is a xeno-protein that can trigger an immune reaction from the recipient of a therapeutic procedure. This study aimed to compare the use of FBS with PRP, which originating from the human sources on the proliferation and the stemness of CD34+ HSC. Experimental research was carried out by measuring several parameters, namely the calculation of the number of cells with the blue trypan exclusion method, the stemness of CD34+ HSC using cytometric flow, and cell differentiation which was assessed by observing cells in Giemsa staining. The results showed that 15% of PRP supplementation could increase the proliferation of CD34+. Flow cytometry analysis showed that each dose of PRP supplementation did not maintain the CD34+ SPH function with CD34+ purity reduction of 31.7%; 31.7%; 21.7% in sequence of PRP5%, 10%, and 15% supplementation. The mononuclear cells which were found in Giemsa staining showed that HSC differentiation occurs into more specific cells. Therefore, it can be concluded that 15% PRP is the best supplement concentration of in SPH proliferation in this experiment."

"[ABSTRAKLatar belakang : Rekonstruksi tulang pada regio kraniofasial membutuhkan bahan tandur sebagai matriks dalam proses regenerasi tulang, untuk mereplikasi struktur tulang yang hilang. Membran perikardium bovine adalah biomaterial yang kaya akan kolagen yang merupakan unsur utama matriks ekstraselular tulang. Bagaimana perilaku osteoblas terhadap bahan membran perikardium bovine produksi BATAN, Jakarta, Indonesia masih belum di teliti.Tujuan : Mengevaluasi perilaku osteoblas manusia MG63 dalam proses regenerasi tulang setelah ditambahkan dengan membran perikardium bovine (Batan, Jakarta, Indonesia).Metoda : Sel osteoblas manusia MG63 dibiakan sampai jumlah mencukupi, kemudian dibagi menjadi 2 kelompok, kelompok pertama ditambahkan dengan membran perikardium bovine dan kelompok kedua tanpa perlakuan sebagai kontrol. Dilakukan pengukuran proliferasi sel osteoblas dalam 24 jam dengan MTT assay. Ekspresi osteokalsin dan deposisi ion kalsium dievaluasi pada hari ke 7, 14, 21, dan 28 setelah perlakuan.Hasil : Membran perikardium bovine meningkatkan rerata proliferasi sel osteoblas, menurunkan level ekspresi osteokalsin pada tahap akhir kalsifikasi sel yang mengindikasikan perlambatan proses down regulation kalsifikasi sel osteoblas, serta meningkatkan deposisi ion kalsium pada biakan sel osteoblas manusia MG63.Kesimpulan : Membran perikardium bovine produksi BATAN, Jakarta, Indonesia meningkatkan proses diferensiasi dan mineralisasi sel osteoblas.

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ABSTRACTBackground : Bone reconstruction of the craniofacial region requires graft material for the bone regeneration process, to replicate structure of the bone. As a graft biomaterial, Bovine pericardium membrane is rich in collagen fibers, which is the main element of bone extracellular matrix. The human cell line behavior in regeneration process after transplantation of bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has not been reported.Objective : The objective of this study was to evaluate the behavior of human osteoblast cell line MG63 in bone regeneration process, after transplantation of bovine pericardium membrane (BATAN, Jakarta, Indonesia).Method : Human osteoblast cell line culture was divided into 2 groups, first group transplanted with bovine pericardium membrane and second group without bovine pericardium membrane as a control. After 24 hours, the proliferation of osteoblast cell are analyzed using MTT assay test, 7, 14, 21, 28 days after transplantation, expression of osteocalcin and deposition of Ca++ was evaluated.Results: Bovine pericardium membrane improved the mean proliferation of osteoblast, lowering the expression level of osteocalcin, that indicate a slowdown in down-regulation process of osteoblast cells calcification, and increase deposition of Ca++ in human osteoblast cell line MG63.Conclusions : Bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has to increase differentiation and mineralization of osteoblast cell.;Background : Bone reconstruction of the craniofacial region requires graft material for the bone regeneration process, to replicate structure of the bone. As a graft biomaterial, Bovine pericardium membrane is rich in collagen fibers, which is the main element of bone extracellular matrix. The human cell line behavior in regeneration process after transplantation of bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has not been reported.Objective : The objective of this study was to evaluate the behavior of human osteoblast cell line MG63 in bone regeneration process, after transplantation of bovine pericardium membrane (BATAN, Jakarta, Indonesia).Method :Human osteoblast cell line culture was divided into 2 groups, first group transplanted with bovine pericardium membrane and second group without bovine pericardium membrane as a control. After 24 hours, the proliferation of osteoblast cell are analyzed using MTT assay test, 7, 14, 21, 28 days after transplantation, expression of osteocalcin and deposition of Ca++ was evaluated.Results: Bovine pericardium membrane improved the mean proliferation of osteoblast, lowering the expression level of osteocalcin, that indicate a slowdown in down-regulation process of osteoblast cells calcification, and increase deposition of Ca++ in human osteoblast cell line MG63.Conclusions : Bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has to increase differentiation and mineralization of osteoblast cell., Background : Bone reconstruction of the craniofacial region requires graft material for the bone regeneration process, to replicate structure of the bone. As a graft biomaterial, Bovine pericardium membrane is rich in collagen fibers, which is the main element of bone extracellular matrix. The human cell line behavior in regeneration process after transplantation of bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has not been reported.Objective : The objective of this study was to evaluate the behavior of human osteoblast cell line MG63 in bone regeneration process, after transplantation of bovine pericardium membrane (BATAN, Jakarta, Indonesia).Method :Human osteoblast cell line culture was divided into 2 groups, first group transplanted with bovine pericardium membrane and second group without bovine pericardium membrane as a control. After 24 hours, the proliferation of osteoblast cell are analyzed using MTT assay test, 7, 14, 21, 28 days after transplantation, expression of osteocalcin and deposition of Ca++ was evaluated.Results: Bovine pericardium membrane improved the mean proliferation of osteoblast, lowering the expression level of osteocalcin, that indicate a slowdown in down-regulation process of osteoblast cells calcification, and increase deposition of Ca++ in human osteoblast cell line MG63.Conclusions : Bovine pericardium membrane produced by BATAN, Jakarta, Indonesia has to increase differentiation and mineralization of osteoblast cell.]"