Hasil Pencarian  ::  Simpan CSV :: Kembali

"This book includes, ABPP methodology (introduction and overview), activity-based protein profiling for natural product target discovery, photoaffinity labeling in activity-based protein profiling, and, functional analysis of protein targets by metabolomic approaches. "

"Latar Belakang: Patogenesis ECC disebabkan sifat virulensi dari protein-protein yang menyusun sel Streptococcus mutans. Tujuan: Mengetahui perbedaan profil protein S. mutans isolat saliva pasien ECC. Metode: Profil protein S. mutans berupa pita protein yang terlihat pada gel poliakrilamida diperoleh melalui metode SDS PAGE. Hasil: Profil protein S. mutans diperoleh secara kualitatif melalui interpretasi pita-pita protein yang merepresentasikan berat molekul 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa, dan 94,5 kDa dengan perbedaan frekuensi ekspresi protein pada pasien ECC dan bebas karies. Kesimpulan: Pada pasien ECC dan bebas karies ditemukan adanya perbedaan profil protein dari S. mutans isolat saliva.

---

Background: The pathogenesis of ECC is caused by virulence properties from proteins which construct the cell of Streptococcus mutans.

Objective: To find out the difference of protein profiling from salivary S. mutans in ECC and free caries.

Methods: Protein profiling of salivary S. mutans appeared on polyacrilamid gel as protein bands obtained through SDS PAGE.

Result: The profile obtained through interpretation of protein bands represent molecular mass 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa, and 94,5 kDa which had different frequencies in protein expression from ECC and free caries subjects.

Conclusion: There is difference in protein profiling of salivary S. mutans both in ECC and free caries subjects."

"Latar Belakang: Patogenesis ECC dipengaruhi oleh salah satu faktor virulensi Streptococcus mutans yang berasal dari protein S.mutans. Tujuan : Menganalisis perbedaan profil protein S.mutans diisolasi dari permukaan lidah pasien ECC dan bebas karies. Metode : Profil protein S.mutans diisolasi dari permukaan lidah diperoleh melalui metode SDS PAGE dan dibaca melalui pita protein yang terlihat pada gel poliakrilamida. Hasil : Pita protein terlihat pada gel poliakrilamida. Terlihat perbedaan frekuensi ekspresi protein S.mutans pada 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa dan 94,5 kDa pasien ECC dan bebas karies. Kesimpulan : Terdapat perbedaan profil protein S.mutans yang diisolasi dari permukaan lidah pasien ECC dan bebas karies.

---

Background: ced by one of virulence factors from protein S.mutans.

Objective: To analyze the difference of S.mutans protein profiling which is isolated from tongue surface in ECC dan free caries subjects.

Method: Protein Profiling of S.mutans isolated from tongue surface was obtained from SDS PAGE method. It was read by protein band which expressed on polyacrylamide gel.

Result: Protein band was present on polyacrylamide gel. This study found the different frequencies in protein expression of S.mutans 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa dan 94,5 kDa in ECC and free caries subjects.

Conclusion: There is difference of S.mutans protein profiling isolated from tongue surface in ECC and free caries subjects."

"Latar BelakangSejak awal pandemi, dinamika mutasi pada domain RBD pada protein S-glycoprotein SARS-CoV-2 telah mengubah patogenisitas varian yang beredar di Indonesia. Penelitian ini akan menganalisis tren mutasi pada berbagai sampel domain RBD di Indonesia yang telah dipublikasikan di Genomic Database dengan menggunakan genomic profilling MetodePasien yang terinfeksi COVID-19 di Indonesia yang sampelnya telah dipublikasikan di database genomik dipilih untuk penelitian. Data berikut akan menjalani beberapa protokol bioinformatika, divisualisasikan ke dalam pohon filogenetik, rendering 3D, dan penilaian dampak mutasi untuk dianalisis.HasilTerdapat 25 clade unik dan 318 RBD unik di Indonesia, mulai dari sampel paling awal hingga tahun 2022. T478K merupakan mutasi RBD yang paling sering, sedangkan 22B merupakan clade yang paling banyak diamati di Indonesia. Varian omicron menunjukkan skor docking yang lebih rendah dan destabilisasi protein yang lebih tinggi serta Kd yang lebih tinggi daripada galur tipe delta dan liar.KesimpulanHasil dari penelitian tersebut menunjukkan tren penurunan patogenisitas virus kemungkinan sebagai pertukaran untuk peningkatan penularan karena mutasi pada RBD selama bertahun-tahun.

---

Introduction

Since the beginning of the outbreak, the dynamic mutations on the RBD domain in the SARS-CoV-2 spike protein have altered the pathogenicity of variants circulating in Indonesia. This research analyzed the mutation trend on the various sample RBD domains in Indonesia published in Genomic Databases using genomic profiling.

Method

Patients infected with COVID-19 in Indonesia with samples published in genomic databases are selected for the research. The following data underwent several bioinformatics protocols, visualized into phylogenetic trees, 3D rendering, and assessment of mutational impact for analysis.

Results

There are 25 unique clades and 318 unique RBD in Indonesia, ranging from the earliest sample to 2022. T478K was the most frequent RBD mutation, while 22B was the most abundant clade observed in Indonesia. The omicron variant showed a lower docking score, higher protein destabilization, and higher Kd than the delta and wild-type strains. Conclusion

The results from the study suggested a decreasing trend in the virus pathogenicity as a potential trade-off to increased transmissibility due to the mutations in RBD throughout the years."

"Latar Belakang : Early Childhood Caries ECC adalah penyakit kronis gigi dengan prevalensi tinggi. ECC disebabkan beberapa faktor seperti pH saliva dan profil protein S.mutans. Tujuan : Mengetahui perbedaan pH saliva dan profil protein S.mutans yang diisolasi dari plak gigi penderita ECC dan bebas karies. Metode : pH saliva diukur menggunakan indikator pH dan profil protein S.mutans diperoleh melalui metode Sodium Deodecyl Sulfate Polyacrylamide Gel ElectropHoresis SDS PAGE. Profil protein S.mutans dibaca melalui pita protein yang terlihat pada gel poliakrilamida. Hasil : pH saliva yang terlihat adalah pH 5, 5.5, 6, 6.5 dan 7. Pita protein yang terlihat memiliki berat molekul 13 kDa, 29 kDa, 39 kDa, 41,3 kDa, 74 kDa dan 95 kDa penderita ECC dan bebas karies. Kesimpulan : Terdapat perbedaan pH saliva dan profil protein S.mutans yang diisolasi dari permukaan gigi penderita ECC dan bebas karies.

---

Backgorund : Early Childhood Caries ECC is a dental chronic disease which has a high prevalence. ECC is caused by several factors, such as saliva pH and S.mutans protein profiling.

Objective : To identify the difference of saliva pH and S.mutans protein profiling which isolated from plaque in ECC dan caries free subjects.

Methods : The saliva pH is measured with pH paper. Protein Profiling of S.mutans was obtained from Sulfate Polyacrylamide Gel ElectropHoresis SDS PAGE. It was read by protein band which expressed on polyacrylamide gel.

Result : The saliva pH shown are 5, 5.5, 6, 6.5 and 7. Protein band shown with molecular mass 13 kDa, 29 kDa, 41,3 kDa, 74 kDa and 95 kDa.

Conclusion : There is difference of saliva pH and S.mutans protein profiling isolated from plaque in ECC and caries free subjects."

"This book is about recent research in the area of profiling humans from their voice, which seeks to deduce and describe the speaker's entire persona and their surroundings from voice alone. It covers several key aspects of this technology, describing how the human voice is unique in its ability to both capture and influence the human persona -- how, in some ways, voice is more potent and valuable then DNA and fingerprints as a metric, since it not only carries information about the speaker, but also about their current state and their surroundings at the time of speaking. It provides a comprehensive review of advances made in multiple scientific fields that now contribute to its foundations. It describes how artificial intelligence enables mechanisms of discovery that were not possible before in this context, driving the field forward in unprecedented ways. It also touches upon related and relevant challenges posed by voice disguise and other mechanisms of voice manipulation. The book acts as a good resource for academic researchers, and for professional agencies in many areas such as law enforcement, healthcare, social services, entertainment etc."

"Activity Based Cost System (ABC System) adalah suatu sistem informasi biaya yang berbasiskan aktivitas dan berfokus untuk mengoptimalkan biaya. ABC System juga berfungsi untuk mengukur kinerja unit organisasi, menghitung harga produk atau jasa secara lebih akurat, dan sebagai sistem penunjang keputusan.Activity Based Cost memiliki falsafah bahwa penyebab utama timbulnya biaya adalah aktivitas. ABC System memungkinkan pengendalian biaya melalui pengelolaan aktivitas. Hubungan antara aktivitas dan biaya inilah yang menjadi dasar dari penerapan ABC System. Laporan proyek mahasiswa ini berisi penjelasan mengenai pengembangan ABC System di CV Litasari. ABC System yang dibangun disesuaikan dengan keadaan CV Litasari sebagai perusahaan manufaktur yang memproduksi pakaian. Implementasi sistem dilakukan dengan bahasa pemrograman ASP.NET dan Microsoft SQL Server 2000 sebagai DBMS."