Hasil Pencarian  ::  Simpan CSV :: Kembali

"Objective: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of aging and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer. Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method. Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p<0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p < 0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p < 0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression.;"

"Objective: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of aging and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer.

Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method.

Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p < 0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p < 0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p < 0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7.

Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression."

"Pengalaman Perempuan yang Memiliki Masalah Seksual Setelah Pengobatan Kanker Serviks: Pembelajaran dari Wanita Indonesia. Kanker serviks merupakan kanker yang umum terdapat pada perempuan. Penyakit ini adalah penyebab utama kedua kematian wanita di Asia serta di Indonesia. Pengobatan kanker serviks juga menimbulkan masalah untuk jangka panjang seperti adaptasi fisik, psikologis, seksual, dan adaptasi sosial. Tujuan penelitian ini adalah untuk menggambarkan dan menafsirkan pengalaman perempuan Indonesia yang telah mengalami disfungsi seksual setelah pengobatan kanker serviks. Tiga belas perempuan Indonesia yang telah berpartisipasi dalam penelitian ini menggambarkan pengalamannya pada dua tahun pertama setelah pengobatan kanker serviks. Data dikumpulkan melalui wawancara mendalam. Empat tema yang diidentifikasi (1) secara fisik dan psikologis terhadap keluhan seksual setelah pengobatan kanker; (2) efek negatif dari pengobatan kanker terhadap hubungan intim dengan pasangan mereka; (3) upaya untuk mengatasi masalah seksual; dan (4) kebutuhan perempuan terhadap bantuan untuk membantu meningkatkan kesehatan seksual mereka. Penelitian ini menawarkan wacana baru terkait pengalaman perempuan Indonesia yang memiliki masalah kesehatan seksual setelah pengobatan kanker serviks. Penelitian ini mampu menyediakan perawat dan berbagai perawatan kesehatan lainnya dengan pemahaman yang lebih baik dari pengalaman, keprihatinan dan kebutuhan penderita kanker.

---

Cervical cancer remains the most common cancer among women. It is the second major cause of women?s deaths in Asia as well as in Indonesia. Cervical cancer treatment also raises issues of long-term physical, psychological, sexual, and social adaptation. The purpose of the study was to describe and to interpret the experiences of Indonesian women who have experienced sexual dysfunction after cervical cancer treatment. Thirteen Indonesian women who were participated in this study described their experiences on the first to two years after cervical cancer treatment. Data were collected through in-depth interviews. Four themes were identified (1) physical and psychological sexual complaints after cancer treatment; (2) negative effects of cancer treatment towards intimate relationship with their spouse; (3) efforts to overcome sexual problems; and (4) women?s needs for help to improve their sexual health. These study findings offer providing new insights into the experiences of Indonesian women with sexual health problems following cervical cancer treatment. This study can provide nurses and other health care providers with better understanding of the experiences, concern and needs of the cancer survivors."

"Penyakit masih menjadi permasalahan yang serius di seluruh dunia, tidak terkecuali Indonesia. Salah satu jenis penyakit yang menjadi penyebab kematian manusia nomor satu di dunia adalah kanker, dengan kanker paru-paru yang menduduki peringkat pertama. Untuk mengetahui kondisi seseorang memiliki sel kanker atau tidak di dalam tubuhnya, diperlukan pemeriksaan seperti pemeriksaan fisik, CT-Scans dan MRI. Pemeriksaan ini digunakan oleh dokter dalam melakukan diagnosa pasien terhadap penyakit kanker. Walaupun telah dilakukan pemeriksaan secara detail, masih sering ditemukan kekeliruan atau kesalahan yang dilakukan oleh dokter dalam memberikan diagnosa. Oleh karena itu, semakin berkembangnya zaman banyak metode yang dapat digunakan dalam mengatasi permasalahan tersebut, salah satunya dengan metode Convolutional Neural Networks. Metode ini merupakan perkembangan dari metode Neural Networks dan akan digunakan dalam melakukan klasifikasi kanker paru-paru berdasarkan data citra MRI. Convolutional Neural Networks memiliki performa yang lebih baik daripada Neural Networks dalam mengklasifikasikan serta memprediksi data kanker paru-paru berdasarkan citra MRI. Hal ini dibuktikan dengan nilai akurasi yang diperoleh dari metode Convolutional Neural Networks lebih besar daripada nilai akurasi metode Neural Networks, yaitu 90% untuk Convolutional Neural Networks dan 66.7% untuk Neural Networks.

---

Disease still a serious problem throughout the world, include Indonesia. One types of diseases that is the number one cause of human death in the world is cancer, with lung cancer ranked first. To find out the condition of a person has cancer cells or not in the body, it requires examinations such as physical examination, CT-Scans and MRI. This examination is used by doctors in diagnosing patients for cancer. Although a detailed examination has been carried out, it is still often found errors was made by the doctor in provided a diagnosis. Therefore, the development of this era, many methods that can be used to overcome these problems, one of them is the Convolutional Neural Networks method. This method is a development of the Neural Networks method and will be used in classifying lung cancer based on MRI image data. Convolutional Neural Networks has better performance than Neural Networks in classifying and predicting lung cancer data based on MRI images. This is evidenced by the accuracy value obtained from the Convolutional Neural Networks method greater than the accuracy value of the Neural Networks method, which is 90% for Convolutional Neural Networks and 66.7% for Neural Networks."

"ABSTRAKSalah satu masalah kesehatan yang disebabkan oleh penyintas kanker ginekologi adalah akibat kekambuhan. Takut kambuh kanker merupakan salah satu masalah psikologis yang paling penting di antara penyintas kanker. Tujuan penelitian ini adalah penyelidikan komprehensif tentang ketakutan kambuh para perempuan penyintas kanker ginekologi. Penelitian ini menggunakan studi deskriptif kualitatif dengan analisis tematik yang menghasilkan tema-tema. Penelitian ini mengungkap berbagai pengalaman 10 partisipan yang mengalami kesulitan kambuh. Hasil temuan pada penelitian ini mengungkap berbagai macam pertanyaan mengenai kesulitan kambuh termasuk faktor pemicunya dan cara mengatasi kesulitan kambuh serta harapan tentang masa depan mereka setelah menyelesaikan terapi kanker.

---

ABSTRACTSalah satu masalah kesehatan yang terjadi pada survivor kanker ginekologi adalah kambuh. Ketakutan kambuh adalah salah satu masalah psikologi yang paling penting di antara penderita kanker. Rasa takut kambuh ini akan muncul berbagai ekspresi. Puporse dari penelitian ini adalah untuk mengeksplorasi secara mendalam tentang persepsi terhadap rasa takut kambuh pada penderita kanker ginekologi yang selamat. Penelitian ini adalah deskriptif kualitatif dengan Analisis Tematik yang menghasilkan tema. Studi ini mengungkap pengalaman sepuluh partisipan yang mengalami rasa takut kambuh. Temuan penelitian ini mengungkapkan berbagai ekspresi dari rasa takut yang kambuh termasuk faktor pemicu dan cara untuk mengatasi ketakutan yang meningkat dan diceritakan oleh peserta termasuk harapan tentang masa depan mereka setelah menyelesaikan terapi kanker."

"Aim: to learn the role of docetaxel in non-castrate resistant prostate cancer patient. Methods: literature search was conducted to find relevant study comparing the combination of docetaxel and androgen deprivation therapy (ADT) to ADT alone in non-castrate resistant prostate cancer using PubMed, Cohrane Library, Proquest, EBSCO, and Scopus database. Quality assessment of studies was done using Bond University Rapid Critical Appraisal of a Systematic Review. Results: we found 494 studies from literature search, but only two studies were included in final selection. Based on validity assessment, we chose one study to be discussed further. This study showed that combination of docetaxel and ADT is better than ADT alone in regards of overall survival (HR 0.64; 95% CI 0.55, 0.75; p<0.0001; NNT=3), biochemical progression free survival (HR 0.63; 95% CI 0.57, 0.69; p<0.0001; NNT=2) and clinical progression free survival (HR 0.73; 95% CI 0.64, 0.84; p<0.0001; NNT=2). Benefit of docetaxel and ADT combination was especially seen in high volume disease (HR 0.67; 95% CI 0.54, 0.83; p=0.0003; NNT=3). Conclusion: addition of docetaxel into ADT has beneficial effects in terms of overall survival and progression free survival in patients with non-castrate resistant metastatic prostate cancer."

"Although the most common cause of lung cancer is long-term exposure to tobacco smoke, the role of genetic factor for the cell defense mechanism, such as MnSOD, should also be considered. This study aims to analyze the expression and genotype of MnSOD in lung cancer cells of smoker patients. Samples were normal and lung cancer cells of patients operated in Persahabatan Hospital from May to December 2008, as well as lung cancer cells extracted from FFPE collection. Leukocyte cells of healthy smoker subjects were used as controls. The MnSOD mRNA expression was analyzed using Real Time RT-PCR and the specific activity using xantin oxidase inhibition assay. The genotyping was performed using PCR-RFLP.The result showed that the MnSOD specific activity in lung cancer of smoker patients is higher than in leukocyte cells of smoker controls. Compared to the expression of MnSOD in the normal lung cells of patients, in the lung cancer cells the level of MnSOD mRNA was lower, whereas its specific activity was higher (1.988 times). The samples from lung cancer patients have a Val/Val genotype frequency of 100%. In this study, we could conclude that MnSOD expression is altered in lung cancer cells."

"Telah dilakukan penelitian mengenai perbandingan ekspresi miRNA-143 pada serum dan sel eksfoliatif serviks serta hubungannya dengan ekspresi Bcl-2. Penelitian ini bertujuan untuk mengetahui perbedaan tingkat ekspresi miRNA-143 antara sampel serum dengan sampel sel eksfoliatif serta hubungannya dengan gen target Bcl-2. Data tersebut dapat digunakan sebagai informasi untuk pengembangan metode noninvasif untuk diagnosis awal kanker serviks. Penelitian dilakukan dengan mengambil sampel serum dan sampel sel eksfoliatif pada subyek normal dan subyek yang terdeteksi kanker serviks kemudian dianalisis dengan menggunakan qRT-PCR. Sampel pasien kanker serviks sebanyak 15 subyek dan 4 subyek normal digunakan untuk mendapatkan nilai kuantitas relatif ekspresi miRNA-143 dan Bcl-2. Analisis tingkat ekspresi miRNA-143 pada sampel serum menunjukkan nilai rerata yang rendah pada subyek normal dibandingkan pasien kanker serviks. Tingkat ekspresi miRNA-143 pada sampel sel menunjukkan hasil berbeda, dengan semakin tinggi derajat keparahan kanker, didapatkan hasil nilai rerata miRNA-143 yang lebih rendah. Analisis distribusi sampel menunjukkan terdapat perbedaan bermakna antara tingkat ekspresi miRNA-143 pada sampel serum dengan sampel sel p < 0,05; Kruskall Wallis . Hubungan antara miRNA-143 dengan gen target Bcl-2 pada penelitian ini menunjukkan korelasi yang lemah dan tidak signifikan r = -0,101; p > 0.05; Pearson .

---

Research had been done on the comparison of miRNA 143 expression in serum and exfoliative cervix cells and its relationship with expression of Bcl 2. This study aims to determine differences in the expression levels of miRNA 143 between serum samples and cell exfoliative samples and its relationship with the target gene Bcl 2. Those data can be used as information for the development of non invasive method for the early diagnosis of cervical cancer. The study was conducted by taking samples of serum and cell exfoliative samples in normal subjects and subjects with cervical cancer, then it is analyzed using qRT PCR. 15 samples of cervical cancer patients were obtained, and 4 normal subjects used to obtain the relative expression levels of miRNA 143 and Bcl 2. Analysis of the expression levels of miRNA 143 in the serum samples showed lower average value in normal subjects compared to patients with cervical cancer . MiRNA 143 expression levels in cell samples showed different results with the higher the degree of severity of the cancer, the average value of the miRNA 143 were lower. Analysis of the samples distribution showed that there are significant difference between the expression levels of miRNA 143 in the serum samples with the cell samples p 0.05, Pearson ."

"Tujuan: Penelitian ini bertujuan untuk mengetahui distribusi dan karakteristik kejadian COVID-19 pada pasien dan tenaga kesehatan/pekerja di IPTOR RSUPN dr. Cipto Mangunkusumo dan juga dampak COVID-19 terhadap luaran terapi berdasarkan kriteria RECIST dan toksisitas radiasi berdasarkan kriteria RTOG pada pasien kanker serviks yang menjalani radioterapi di IPTOR RSUPN dr. Cipto Mangunkusumo.Metode: Studi eksploratif retrospektif dari 835 pasien kanker serviks yang terdaftar di IPTOR RSUPN dr. Cipto Mangunkusumo, dari Juli 2020 hingga Maret 2022. Melalui analisis kriteria inklusi dan eksklusi, didapatkan sampel 506 pasien yang terdiri dari 77 pasien terkonfirmasi COVID-19 dan 429 pasien yang tidak terpapar COVID-19, serta data insidensi terkonfirmasi COVID-19 dari seluruh pasien kanker, insiden COVID-19 di poliklinik serta insiden terkonfirmasi COVID-19 tenaga kesehatan dan pekerja di IPTOR RSUPN dr. Cipto Mangunkusumo.Hasil: Diketahuinya gambaran infeksi COVID-19 pada pasien dan tenaga kesehatan serta pekerja di RSUPN dr. Cipto Mangunkusumo. Juga, telah dilakukan penilaian luaran terapi berdasakran kriteria RECIST terhadap pasien kanker serviks terkonfirmasi COVID-19 maupun yang tidak terkonfirmasi COVID-19 pada 2 minggu serta ≥ 3 bulan pasca radiasi. Ditemukan bahwa status COVID-19 pada pasien kanker serviks tidak berpengaruh signifikan (p>0,05) terhadap luaran terapi 2 minggu setelah radiasi, namun meningkatkan peluang CR sebesar 1,120 OR (95% CI: 0,645-1,943). Lebih lanjut, pada luaran ≥ 3 bulan pasca radiasi, status COVID-19 berpengaruh signifikan (p<0,05) dan meningkatkan peluang CR sebesar 1,157 OR (95% CI: 1,09-1,223) Sementara itu, juga telah dilakukan penilaian antara toksisitas radiasi terhadap pasien kanker serviks terkonfirmasi COVID-19 maupun yang tidak COVID-19, di mana ditemukan bahwa status COVID-19 tidak berpengatuh signifikan (p>0,05) terhadap toksisitas radiasi, namun status COVID-19 positif ditemukan dapat meningkatkan peluang terjadinya toksisitas radiasi sebesar 1,348 (0,78-2,30) OR (95% CI).Kesimpulan: Berdasarkan pengumpulan dan analisis data dari penelitian ini, ditemukan bahwa infeksi COVID-19 memiliki pengaruh untuk tercapainya luaran terapi yang lebih baik pada pasien kanker serviks yang mendapatkan radioterapi.

---

Objective: This study aims to determine the distribution and characteristics of COVID-19 incident in patients and clinicians/workers at IPTOR RSUPN dr. Cipto Mangunkusumo. Also, this study aims to investigate the impact of COVID-19 on radiotherapy outcome based on RECIST criteria and radiation toxicity based on RTOG criteria in cervical cancer patients undergoing radiotherapy at IPTOR RSUPN dr. Cipto Mangunkusumo.

Method: An exploratory retrospective study of 835 cervical cancer patients registered at IPTOR RSCM, from July 2020 to March 2022. Through analysis of inclusion and exclusion criteria, a sample of 506 patients was obtained, consisting of 77 COVID-19 confirmed patients and 429 non-exposed COVID-19 patients, as well as data on the incidence of COVID-19 confirmed cases among cervical cancer patients, outpatients, and healthcare workers and staff at IPTOR RSCM.

Results: This study showed an overview of COVID-19 infections in all patients and hospital staffs from Departement of Radiation Oncology Cipto Mangunkusumo Hospital. Also, in this study we assess the radiotherapy outcome according to RECIST criteria for cervical cancer patients with COVID-19 and those who were not confirmed with COVID-19 at 2 weeks and ≥ 3 months after radiation. We found that COVID-19 in patients with cervical cancer had no significant impact (p>0.05) on therapy outcomes 2 weeks after radiation, but it increased the chance of CR by 1.120 OR (95% CI: 0.645-1.943). For RECIST ≥ 3 months post-radiation, COVID-19 status had a significant impact (p<0.05) and increased the chance of CR by 1.157 OR (95% CI: 1.09-1.223). Meanwhile, an assessment was also carried regarding radiation toxicity in cervical cancer patients confirmed with COVID-19 and those without COVID-19, and it was found that COVID-19 status did not have a statistically significant impact (p>0.05) on RTOG toxicity but positive COVID-19 status increased the chance of radiation-related toxicity by 1.348 (0.78-2.30) OR (95% CI).

Conclusion: Based on the data from this study, COVID-19 infection has an impact on the outcomes of cervical cancer patients who receive radiation therapy."