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"Pengaruh aplikasi non freeze-dried hidrogel-CHA terhadap prolifrasi fibroblas. Kerusakan tulang dapat disebabkan oleh berbagai prosedur bedah. Rekonstruksi tulang yang banyak dikembangkan akhir-akhir ini adalah teknik rekayasa jaringan. Material yang terbukti efektif sebagai perancah dalam rekayasa jaringan adalah hidrogel. Penambahan Karbonat apatit (CHA) akan menghasilkan material hidrogel-CHA yang diyakini dapat meningkatkan sifat mekanis dan kemiripan biologis dengan tulang. Perancah merupakan aspek penting dalam bidang rekayasa jaringan, karena kemampuannya menyerupai matriks ekstraseluler pada jaringan yang rusak. Fibroblas merupakan sel mesenkim yang dengan mudah dibiakkan di laboratorium dan memiliki peran penting dalam interaksi epitel-mesenkimal, mensekresi berbagai faktor pertumbuhan dan sitokin. Pada kondisi tertentu fibroblas akan berdiferensiasi menjadi sel pembentuk tulang yaitu osteoblas. Tujuan: untuk mengetahui pengaruh non freeze-dried hidrogel-CHA terhadap jumlah sel fibroblas. Metode: Pada kelompok perlakuan (kelompok hidrogel dan hidrogel-CHA), penyemaian statis yaitu sel dan perancah dikontakkan. Pada kelompok lain hanya berisi sel dan media pertumbuhan. Sel disemai dengan kepadatan 2x104 sel/ml dalam 96 sumuran. Jumlah sel fibroblas dalam tiap kelompok diamati dengan menggunakan mikroskop dan dihitung dengan uji MTT padahari 1, 2,dan 3 setelah aplikasi perlakuan. Hasil: Proliferasi jumlah sel fibroblas meningkat secara signifikan pada hari ke-3 setelah aplikasi non freeze-dried hidrogel-CHA (p<0,05). Simpulan: Aplikasi non freeze-dried hidrogel-CHA dapat meningkatkan proliferasi sel fibroblas.

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Bone damage can be caused by variety of surgical procedures. Bone reconstruction has been developed lately is tissue engineering techniques. One of materials that proved to be effective as a scaffold in tissue engineering is a hydrogel. The addition of carbonate apatite (CHA) will produce a hydrogel-CHA material which is believed to improve the mechanical properties and biological similarities with the original bone. Scaffold is considered an important aspect in the field of tissue engineering, because it’s ability to mimic extracellular matrix of the damaged tissue. Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelial-mesenchymal interactions, secreting various growth factors and cytokines. On certain condition, Fibroblast will differentiate into bone-forming cells, osteoblasts. Objective: to determine the effect of non freezedried hydrogels - CHA on the number of fibroblasts. Methods: In the treatment groups (hydrogel and hydrogel - CHA group), the static seeding, where cells and scaffolds were simply brought into contact, was performed. The other group contained only cells and growth media. Cells were seeded at a density of 2x104 cells/ml in a 96-well plate. Number of fibroblasts cell in each group was observed by light microscopy and quantitified by MTT assay on days 1, 2 and 3 post-application. Results: Proliferation of fibroblasts increased significantly on day 3rd after application of non freeze-dried hydrogel - CHA (p< 0.05). Conclusion: Application of non freeze-dried hydrogel - CHA may induce fibroblasts proliferation."

"ABSTRAKPendahuluan: Keloid adalah tumor jinak pada kulit yang ditandai denganpeningkatan deposisi kolagen yang disebabkan oleh tingginya aktivitas danproliferasi fibroblas. Karakteristik utama dari keloid adalah berada pada kondisihipoksia. Hypoxia inducible factor-1alpha HIF-1? dan HIF-2? merupakansubunit faktor transkripsi HIF-1 dan HIF-2 yang berperan penting pada adaptasiterhadap kondisi hipoksia. Kondisi hipoksia juga memicu sel memproduksireactive oxygen species ROS yang dapat ditangkal oleh sitoglobin Cygb . Padakeloid, Cygb juga berperan pada sintesis kolagen. Penelitian lain membuktikanbahwa terdapat situs pengikatan promoter HIF-1 pada hypoxia response element HRE Cygb, sedangkan situs pengikatan HIF-2 pada Cygb belum diketahui. HIF-1? dan HIF-2? juga diketahui berperan menginduksi ekspresi gen yang berperanpada proliferasi. Keduanya diketahui mengatur proliferasi sel pada beberapa jeniskanker. Namun, peran HIF-1? dan HIF-2? terhadap ekspresi Cygb dan proliferasifibroblas pada keloid belum diketahui. Oleh karena itu, penelitian ini bertujuanmengetahui peran HIF-1? dan HIF-2? terhadap ekspresi sitoglobin dan proliferasifibroblas pada keloid, dengan melakukan penghambatan HIF-1? dan HIF-2? padafibroblas keloid menggunakan inhibitor HIF berupa ibuprofen. Metode:Pemeriksaan dilakukan terhadap ekspresi mRNA dan kadar protein HIF-1?, HIF-2?, dan sitoglobin, menggunakan metode qRT-PCR dan ELISA, serta proliferasisel menggunakan metode trypan blue exclussion assay setelah diberi ibuprofendan dibandingkan dengan kontrol yang tidak diinduksi dengan ibuprofen. Hasil:Penghambatan HIF-1? menyebabkan terjadinya penurunan ekspresi mRNA Cygbdan proliferasi fibroblas pada keloid. Hal ini membuktikan bahwa HIF-1?berperan sebagai faktor transkripsi Cygb, dan juga berperan menginduksi ekspresigen yang berperan pada proliferasi fibroblas keloid. Akan tetapi, peran HIF-2?dalam meregulasi sitoglobin dan mempertahankan proliferasi fibroblas keloidbelum berhasil dibuktikan. Kesimpulan: HIF-1? berperan meregulasi eksrepsiCygb dan berperan pada proliferasi fibroblas keloid. Akan tetapi, HIF-2? belumterbukti meregulasi eksrepsi Cygb dan berperan pada proliferasi fibroblas keloid.

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ABSTRACTBackground Keloid is a benign tumor which is characterized by overabbundance of collagen deposition caused by high activity and proliferation offibroblast. The main characteristics of keloid is hypoxia. HIF 1 and HIF 2 aretwo subunits of transcrption factors HIF 1 and HIF 2 which mediate adaptationto hypoxic condition. Hypoxic condition also induces cells to produce reactiveoxygen species ROS which can be scavenged by cytoglobin Cygb . In keloid,Cygb also plays important role in collagen synthesis. Other studies prove thatthere is a binding site of HIF 1 promoter on hypoxia response element HRE ofCygb. Whereas, HIF 2 binding site on HRE of Cygb is not cleraly understood.HIF 1 and HIF 2 also play important role to induce expression of many geneswhich involved in cells proliferation. Both HIF 1 and HIF 2 are known toregulate cells proliferation in many cancer types. However, the role of HIF 1 andHIF 2 to Cygb expression and fibroblast proliferation in keloid is not fullyunderstood. Therefore, the aim of this study is to determine the role of HIF 1 andHIF 2 on Cygb expression and fibroblast proliferation in keloid, by doing aninhibition of HIF 1 and HIF 2 in keloid fibroblast primary culture usingibuprofen as HIF inhibitor. Methods Analysis was conducted by analizing HIF 1 , HIF 2 , and Cygb mRNA expression and protein level using qRT PCR andELISA, and fibroblast proliferation analysis was conducted using trypan blueexclusion assay after given with ibuprofen and compared with control which isnot given with ibuprofen. Results Inhibition of HIF 1 caused a decrease onCygb mRNA expression and fibroblast proliferation on keloid. These findingsprove that HIF 1 acts as transcription factor of Cygb, and also inducesexpression of gene which plays a role on fibroblast proliferation in keloid.However, the role of HIF 2 in regulating and maintaining Cygb expression andfibroblast proliferation in keloid was not succesfully proven. Conclusion HIF 1 regulate Cygb expression and fibroblast proliferation in keloid. However, HIF 2 role on Cygb expression and fibroblast proliferation has not be proven yet."

"Penelitian ini bertujuan untuk menguji sitotoksisitas tiga merk dagang eugenol pada fibroblast gingiva manusia, untuk mengetahui eugenol yang paling tidak toksik terhadap jaringan bila dipakai untuk pengobatan gigi. Dari ketiga merk dagang eugenol, Kimia Farina (El), Dentsply (E2), dan Cavex (E3) dibuat campuran medium-eugenol masing masing menjadi 0,031%, 0,062%, 0,125%, 0,25%, 0,5%, 1%, 2%, dan 4%. Dilakukan kultur sel primer fibroblast gingiva manusia, dan setelah sel konfluen kemudian dipajan dengan masing-masing konsentrasi medium-eugenol selama 24 jam. Penghitungan jumlah sel yang masih hidup dilakukan dengan uji metode assay MTT. Hasil uji assay MTT menunjukkan bahwa E3 dengan konsentrasi 0,062% mengakibatkan jumlah sel vital sangat menurun, sementara pada El dan E2 penurunan jumlah sel tidak tampak. Sel menjadi tidak tumbuh pada konsentrasi El dan E2 0,25%, dan hal yang sama terjadi pada E3 dengan konsentrasi yang lebih rendah 0,125%. Kesimpulan: El dan E2 merupakan eugenol yang kurang toksik dibandingkan dengan E3."

"[Pendahuluan. Sel punca mesenkimal yang dilaporkan mengaugmentasi penyembuhan fraktur umumnya diperoleh dari sumsum tulang. Donor sel punca dari sumsum tulang terbatas pada volume aspirat dan menimbulkan morbiditas donor sehingga diperlukan sumber alternatif. Darah perifer menutupi kekurangan tersebut walaupun memiliki kandungan sel punca yang lebih sedikit. Pemberian Granulocyte Colony Stimulating Factor (GCSF) dapat meningkatkan mobilisasi sel mononuklear pada teknik afaresis untuk sel punca hematopoetik. Bila pemberian GCSF diikuti dengan teknik kultur kearah sel punca mesenkimal maka dapat meningkatkan jumlah sel punca darah perifer sehingga memungkinkan penggunaan darah perifer sebagai donor alternatif sel punca. Oleh karena itu, diperlukan penelitian untuk mengevaluasi penggunaan darah perifer sebagai donor sel punca pasca pemberian GCSF dengan menilai kemampuan mobilisasi, proliferasi dan diferensiasi.Metode Penelitian. Penelitian ini adalah penelitian eksperimental yang memakai hewan coba 14 ekor kelinci New Zealand White jantan, berat badan 2 kg di Pusat Studi Satwa Primata, Institut Pertanian Bogor. Sampel dibagi secara acak menjadi 4 kelompok yaitu kontrol dan perlakuan (injeksi GCSF dosis 10mcg/kg berat badan, subkutan, selama 7 hari) dimana pada masing-masing kelompok diambil aspirat darah perifer dan sumsum tulang (kelompok 1: kontrol sumsum tulang, kelompok 2: kontrol darah perifer, kelompok 3: perlakuan sumsum tulang, kelompok 4: perlakuan darah perifer). Pada tiap kelompok dilakukan isolasi, ekspansi dan diferensiasi menjadi osteoblas. Analisis statistik menggunakan uji one way Anova dan dilanjutkan uji posthoc untuk jumlah sel inisial, waktu konfluensi, jumlah sel konfluensi dan waktu diferensiasi.Temuan dan Diskusi Penelitian. Sel punca mesenkimal pada seluruh kelompok penelitian mampu diisolasi, berproliferasi dan berdiferensiasi menjadi osteoblas. Rerata jumlah sel inisial kelompok 1: 3.07 x 106/mL, kelompok 2: 2.11 x 106/mL, kelompok 3: 2.89 x 106/mL dan kelompok 4: 7.35 x 106/mL (p< 0.001). Rerata waktu konfluensi kelompok 1: 25.8 hari, kelompok 2: 35.7 hari, kelompok 3: 26 hari, kelompok 4: 19.7 hari (p< 0.001). Rerata jumlah sel konfluensi kelompok 1: 6.54 x 106/mL, kelompok 2: 4.61 x 106/mL, kelompok 3: 5.94 x 106/mL, kelompok 4: 11.14 x 106/mL (p< 0.001). Rerata waktu diferensiasi kelompok 1: 15.5 hari, kelompok 2: 25.4 hari, kelompok 3: 15.4 hari, kelompok 4: 11.2 hari (p< 0.001). Uji posthoc jumlah sel inisial ditemukan perbedaan pada kelompok 1 dan 4 (p= 0.000), 2 dan 4 (p< 0.001), serta 3 dan 4 (p< 0.001). Uji posthoc waktu konfluensi, jumlah sel konfluensi dan waktu diferensiasi didapatkan perbedaan diantara semua kelompok kecuali kelompok 1 dan 3 (p= 1.000, 0.670, 1.000).Simpulan. Sel punca mesenkimal darah perifer pasca induksi GCSF mampu diisolasi, berproliferasi dan berdiferensiasi. Pemberian GCSF meningkatkan jumlah sel punca mesenkimal dan mempersingkat durasi kultur. Darah perifer memberikan harapan baru sebagai donor alternatif sel punca mesenkimal., Introduction. Mesenchymal stem cells, which had been reported to augment fracture healing, were routinely harvested from bone marrow. Bone marrow had several drawbacks regarding its limited aspiration volume and donor site morbidity, therefore alternative donor is needed. Peripheral blood may cover those disadvantages despite the fewer stem cells number. Granulocyte colony stimulating factor (GCSF) administration in aphaeresis technique could mobilized mononuclear cells to hematopoietic stem cells. If it is followed by culture for mesenchymal stem cells expansion, thus will increase peripheral mesenchymal stem cells number therefore might facilitate peripheral blood as an alternative donor. For that reason, further research is needed to evaluate the effect of GCSF induction to peripheral blood as stem cells alternative donor by assessing its capability on mobilization, proliferation and differentiation.Methods. This is an experimental study using 14 male New Zealand White rabbit, weighted 2-3 kg in Primate Research Centre, Bogor Agricultural Institute. Sample was randomized into 4 groups as follow, control and treatment group (GCSF administration, 10mcg/kg body weight, subcutaneous, 7 days) in which peripheral blood and bone marrow aspiration was collected (group 1: control bone marrow, group 2: control peripheral blood, group 3: treatment bone marrow, group 4: treatment peripheral blood). Isolation, expansion and osteoblast differentiation were followed subsequently. Statistical analysis used one-way Anova and posthoc for initial cell number, confluency time, confluency cell number, and differentiation time.Result and Discussion. Mesenchymal stem cells in all groups were able to be isolated, proliferate and differentiate to osteoblast. Initial cell number (mean) group 1: 3.07 x 106/mL, group 2: 2.11 x 106/mL, group 3: 2.89 x 106/mL and group 4: 7.35 x 106/mL (p< 0.001). Confluency time (mean) group 1: 25.8 days, group 2: 35.7 days, group 3: 26 days, group 4: 19.7 days (p< 0.001). Confluency cell number (mean) group 1: 6.54 x 106/mL, group 2: 4.61 x 106/mL, group 3: 5.94 x 106/mL, group 4: 11.14 x 106/mL (p< 0.001). Differentiation time group 1: 15.5 days, group 2: 25.4 days, group 3: 15.4 days, group 4: 11.2 days (p< 0.001). Posthoc analysis for initial cell number was found significantly different for group 1 and 4 (p= 0.000), group 2 and 4 (p< 0.001) and group 3 and 4 (p< 0.001). Posthoc analysis for confluency time, confluency cell number and differentiation time was found significantly different for all groups except group 1 and 3 (p= 1.000, 0.670, 1.000).Conclusion. Peripheral blood mesenchymal stem cells after GCSF induction are able to be isolated, proliferate and differentiate. GCSF administration increase mesenchymal stem cells number and shorten culture duration. Peripheral blood is a promising alternative donor for mesenchymal stem cells.]"

"Artikel ini membahas sepak terjang dari kelompok masyarakat sistematis yang menginginkan terjadinya pemekaran wilayah di Indonesia. Salah satu daerah sebagai hasil dari praktik pemekaran wilayah adalah Kabupaten Bandung Barat. Dalam sejarahnya, pihak yang sangat vokal memperjuangkan usaha tersebut adalah Komite Pembentukan Kabupaten Bandung Barat atau KPKBB. Meninjau kembali perjuangan KPKBB penting karena komite tersebut adalah “legalisasi” dari semua aspirasi dan pergerakan masyarakat yang menuntut pemekaran wilayah ketika itu. Dalam rangka merekonstruksi gejala yang dimaksud, digunakan metode penelitian sejarah yang terdiri dari empat tahapan, yakni heuristik, kritik, interpretasi, dan historiografi. Dalam tahapan heuristik, selain studi pustaka yang mengandalkan buku teks terbitan pemerintah setempat serta tinjauan terhadap artikel jurnal dari berbagai situs, juga digunakan sumber-sumber primer, antara lain arsip dan dokumen yang tersimpan di Depo Arsip Kabupaten Bandung, Depo Arsip Kabupaten Bandung Barat, serta surat kabar sezaman dari Pikiran Rakyat koleksi Perpustakaan Nasional RI. Selain itu, karena tulisan ini bersifat sejarah lokal dan kontemporer, peneliti juga mengandalkan sumber lisan sebagai bahan penelitian dengan mewawancarai beberapa tokoh KPKBB yang masih hidup. Hasilnya, artikel ini menemukan bahwa peranan dari KPKBB signifikan dalam proses percepatan kelahiran Kabupaten Bandung Barat. Maka, tulisan ini akan berfokus pada analisis peran yang dilakukan KPKBB dalam berbagai usahanya untuk mempercepat terjadinya pemekaran wilayah.

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This article discuss the actions of systematic community groups who want regional expansion in Indonesia. One of the areas as a result of the practice of proliferation of (administrative) regions is West Bandung Regency. Historically, the actor who has been very vocal in fighting for the formation of the area is the West Bandung Regency Establishment Committee or KPKBB. Reviewing the KPKBB's struggle is important because the committee was the “legalization” of all community movements that demanded proliferation at that time. In order to reconstruct the phenomenon, historical research methods are used which consist of four stages, namely heuristics, verification, interpretation, and historiography. In the heuristic stage, apart from a literature study that relied on textbooks published by the local government and a review of journal articles from various sites, other sources were used such as archives and documents stored at the Bandung Regency Archives Depot, West Bandung Regency Archives Depot, as well as the Pikiran Rakyat newspaper which is the collection of the National Library of the Republic of Indonesia. In addition, because this paper is a local and contemporary history, the researcher also relies on oral sources as research material by interviewing several KPKBB figures who are still alive. As a result, this article finds that the role of the KPKBB is significant in the process of accelerating the birth of West Bandung Regency. Thus, this paper will focus on analyzing the role played by the KPKBB in its various efforts to accelerate the proliferation."

"Penelitian ini bertujuan untuk mengidentifikasi efek pajanan suhu lingkungan terhadap proliferasi sel dan derajat nekrosis dari adenokarsinoma mammae Pada penelitian true experimental parallel design ini mencit mencit yang telah ditransplantasikan dengan adenokarsinoma mammae dibagi menjadi empat grup dengan masing masing grup dipajankan temperatur lingkungan dengan satu dari beberapa rentang suhu tertentu 20 220C 25 270C 32 340C dan 37 390C selama enam jam hari selama dua minggu Grup temperatur 37 390C dieksklusi karena semua subjek pada grup ini mati Analisis sampel berdasarkan metode AgNOR HE Dari hasil analisis AgNOR ditemukan terdapat perbedaan signifikan dalam hal respon proliferasi sel antara ketiga grup temperatur ANOVA p mAgNOR 0 000 p pAgNOR 0 000 Grup temperatur 32 340C menunjukkan respon proliferasi sel yang lebih besar dibandingkan dengan grup temperatur 20 220C Namun analisis HE gagal menunjukkan perbedaan signifikansi dalam hal respon derajat nekrosis antara ketiga grup temperatur nilai tes Mann Whitne Asymp Sig 2 tailed antara grup temperatur 20 220C dan kontrol 25 270C 0 241 dan nilai tes Mann Whitney Asymp Sig 2 tailed antara grup temperatur 32 340C dan kontrol 0 575 Studi AgNOR menunjukkan bahwa respon proliferasi sel adenokarsinoma mammae memiliki korelasi positif terhadap rentang temperatur Di lain pihak studi HE tidak menunjukkan adanya pengaruh temperatur terhadap derajat nekrosis adenokarsinoma mammae pada mencit

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This research focuses on identifying the effect of environmental temperature exposure on cell proliferation & degree of necrosis of adenocarcinoma mammae. True experimental design (parallel) research was conducted in which the subjects (mice that have been transplanted with adenocarcinoma mammae) were divided into 4 groups with each group was exposed for 2 weeks (6 hours/day) to a environmental temperature of certain range; 20-220C, 25-270C, 32-340C, & 37-390C. In the process, the last group was excluded since all of the subjects in this group died. Sample analysis based on AgNOR & HE method was then done. From the AgNOR study, it was found that there is a significant difference in cell proliferation response between the remaining three temperature groups (ANOVA: p mAgNOR = 0.000; p pAgNOR = 0.000). The high temperature group (32-340C) shows greater cell proliferation compared to the low temperature group (20-220C). However, HE study failed to show significance in the necrosis response between the three temperature groups (Mann Whitney Test: Asymp. Sig (2-tailed) value between low & control group = 0.241; Asymp. Sig (2-tailed) value between control & high temp group = 0.575). In summary, AgNOR study shows that cell proliferation response in adenocarcinoma mammae shows a positive correlation with the temperature ranges. In contrast, HE study shows that temperature of any range has no effect on the degree of necrosis in mice with adenocarcinoma mammae."

"Mineral trioxide aggregate (MTA) as retrograde filling is always in contact to inflamed tissues in periradicular area. Objective: To investigate the effect of acidic environment on push-out bond strength of MTA mixed with sterile water, local anesthetic, and 5% CaCl2 Methods: Thirty middle third of mandibular premolar roots were randomly assigned into 3 groups of 10 each. MTA mixed with sterile water (Group 1), local anesthetic (group 2), 5% CaCl2 (group 3). Each group was then divided into group A: soaked in synthetic tissue fluid with pH 5, and group B: pH 7.4. Specimens were stored in an incubator with a temperature of 370 C for 72 hours, undertaken a push-out test,and observed under a stereo-microscope. Results: A two-way ANOVA showed that acidic environment reduced the push-out bond strength of MTA mixed with either sterile water, local anesthetic or 5% CaCl2 (p <0.05). The predominantly failure was a mixture of adhesive and cohesive type. Conclusion: The acidic environment reduced the push-out bond strength of MTA mixed with either sterile water, local anesthetic or 5% CaCl2. MTA mixed with 5% CaCl2 produced the greatest push-out bond strength, whereas MTA mixed with local anesthetic had the lowest push-out bond strength."

"Human dental pulp of exfoliated deciduous teeth contains the population of cells that exhibited mesenchymal stem cell (MSC) characters. Though, a cell amplification process is indeed required to secure and adequate cell number for such a potential employment. Several publications suggested the alteration of MSCs upon in vitro culture, for example, the decrease in proliferation and the loss of stem cell characters. Here, we investigated an influence of basic fibroblast growth factor (bFGF) on stem cells isolated from human exfoliated deciduous teeth (SHEDs) with respect to cell proliferation, colony forming unit efficiency and stem cell marker expression in both short- and long-term cultures. For short-term bFGF treatment, SHEDs were treated with bFGF for 48h. While, in long-term bFGF supplementation, SHEDs were maintained in culture and continuous passage upon confluence in medium supplemented witg bFGF. Cells at passage (P) 5 and 10 were employed for characterization. Our result showed that short-term bFGF treatment enhanced OCT4, REX1, and NANOG mRNA expression as well as colony forming unit ability. The FGFR inhibitor pretreatment was able to attenuate the influence of bFGF on pluripotent stem cell marker expression, confirming bFGF function. In addition, cells cultured in high passage number had decreased in cell proliferation, colony forming unit capacity, and pluripotent stem cell marker mRNA expression. However, bFGF supplementation in culture medium enhanced both pluripotent stem cell marker expression and colony forming unit capacity in later passage, though the effect was not robust. Together, these results indicate that high passage number may attenuate pluripotent properties of SHEDs and bFGF supplementation could be the beneficial approach to maintain SHEDs' stemness properties. "

"Latar belakang: Studi ini dilakukan untuk mempelajari pengaruh pemberian ekstrak akar Acalypha indica Linn terhadap viabilitas relatif dan proliferasi sel sebagai parameter neurogenesis pada kultur jaringan hipokampus tikus pascahipoksia.Metode: Studi eksperimental in vitro pada 24 kultur primer jaringan sel saraf tikus Sprague Dowley dewasa yang dipajankan terhadap hipoksia dengan gas 5% O2/5% CO2/N2 seimbang selama 24 jam. Pascahipoksia, ekstrak Acalypha indica Linn ditambahkan pada 3 kelompok perlakuan, masing-masing dengan dosis 10, 15, dan 20 mg/mL, sedangkan pada kelompok kontrol tidak ditambahkan apapun. Setiap kelompok terdiri atas 6 sampel. Setelah inkubasi selama 90 jam, viabilitas relatif sel diukur dengan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), proliferasi sel diukur dengan 5-bromo2â??-deoxy-uridine (BrdU). Data dianalisis dengan menggunakan tes parametrik one way ANOVA yang dilanjutkan dengan analisis post-hoc.Hasil: Viabilitas relatif sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (176,95%, 220,62%, 386,02% vs. 100%). Proliferasi sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (0,132; 0,117; 0,114 vs. 0,096).Kesimpulan: Ekstrak Acalypha indica Linn dapat meningkatkan viabilitas relatif dan proliferasi sel pascahipoksia in vitro pada dosis 10, 15, dan 20 mg/mL. (Med J Indones 2011; 20:94-9)

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AbstractBackground: This research was done to study the infl uence of Acalypha indica Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture.Methods Experimental in vitro study using 24 primary neuronal cell cultures obtained from adult Sprague Dawley rat exposed to hypoxia with 5% O2/5% CO2/N2 balance gas for 24 hours. Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. No treatment was given to the control group. Each group consists of 6 samples. After 90 hours of incubation, relative cell viability was measured by using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and cell proliferation was measured by using 5-bromo2â??-deoxy-uridine (BrdU) for cell proliferation. Data was analyzed using one way ANOVA parametric tests, then further analyzed with post-hoc analysis.Results: The relative cell viability of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). Cell proliferation of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (0.132, 0.117, 0.114 vs 0.096).Conclusion: Acalypha indica Linn root extract with doses of 10, 15, and 20 mg/mL can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. (Med J Indones 2011; 20:94-9)"