Hasil Pencarian  ::  Simpan CSV :: Kembali

"Graptophyllum pictum (Daun ungu) is one of the traditional plants in Indonesia. Graptophyllum pictum composition is flavonoid, Flavonoid has an antimicrobial effect. The purpose of this study is to find the effect of Graptophyllum pictum extract prevent the growth of Candida albicans on acrylic resin denture plate. The research was done plaque smear which Candida albicans adhered at the patient denture, then it was cultured and Candida albicans growth was cultivated. The research was done by using 40 samples. The samples were heat cured acrylic resin discs with 10 mm diameter. The research was used dilution method. The samples were divided into four groups.. Each group hadbeen given Graptophyllum pictum extract with 5%, 10%, 20% and 40%for 8 hours. The plates with Sabouraud agar were incubated at 37oC for24 hours and then Candida albicans were counted. The data wereanalyzed by the One way variant analysis and t-test. The result showedthere were significant differences among the amount of Candida albicans which grew in different concentration of Graptophyllum pictumextract (p < 0.05). The conclusion was that Graptophyllum pictumextract had prevented the growth of Candida albicans on acrylic resindenture plate."

"Latar Belakang: Tingginya angka prevalensi denture stomatitis yang terjadi akibat pemakaian gigi tiruan serta pengaruh kestabilan oral candida. Tujuan: Mengamati pengaruh kekasaran bahan basis gigi tiruan terhadap koloni Candida albicans. Metode: mengukur uji kekasaran dengan Roughness tester serta spesimen dicelupkan kedalam eppendorf tube modifikasi yang berisi suspensi Candida albicans diinkubasi dalam waktu 24 dan 72 jam. Data analisis dengan Korelasi Bivariat (Pearson). Hasil: Penurunan jumlah kolonisasi Candida albicans terhadap kekasaran permukaan basis gigi tiruan dipoles dengan tidak dipoles. Terdapat perbedaan jumlah kolonisasi Candida albicans diikuti dengan lama waktu inkubasi. Kesimpulan: Penurunan nilai CFU Candida albicans dipengaruhi oleh penurunan nilai kekasaran permukaan setelah dilakukan pemolesan pada bahan basis gigi tiruan metal, resin akrilik, dan valplast.

---

Background: The high prevalence of denture stomatitis caused by the using of denture and predispose the stability of oral candida.

Objective: The objective of this study is observing the effect of surface roughness of denture base material with the amount of Candida albicans.

Method: measuring surface roughness by using roughness tester and the specimen was immersed int ependorf tube modification with a suspension Candida albicans and incubated for 24 and 72 hour. Data analyzed by Bivariate Correlation (Pearson).

Results: Decrease the amount of Candida albicans colonization of the surface roughness of denture based on polished and not polished. There are differences in the number of Candida albicanos colonization followed by a long incubation time.

Conclusion: The decrease in amount of Candida albicans was affected by the decreasing in the value of the surface roughness after polishing the denture base material metal, acrylic resin, and valplast."

"Ruang lingkup dan Metodologi : Penyebab utama kasus kandidosis adalah Candida albicans. Penanggulangan penyakit ini biasanya dikaitkan dengan pengobatan. Pada umumnya antimikotik yang sering digunakan untuk pengobatan adalah antimikotik golongan azol yaitu ketokonazol dan flukonazol.Penelitian ini bertujuan untuk mengetahui pengaruh ketokonazol dan flukonazol terhadap pertumbuhan Candida albicans. Metode yang digunakan dalam penelitian ini adalah Macrodilution/Tube Method. Pengujian terhadap ketokonazol dilakukan dengan konsentrasi antara 0,25 µg/ml sampai dengan konsentrasi terendah 128 µg/ml dengan waktu pemaparan 1 x 24 jam, 2 x 24 jam dan 3 x 24 jam dalam waktu pengamatan 24 dan 48 jam. Pengujian terhadap flukonazol dilakukan dengan konsentrasi antara 0,1 µg/ml sampai dengan konsentrasi 51,2 µg/ml dengan waktu pemaparan 1 x 24 jam, 2 x 24 jam dan 3 x 24 jam, dalam waktu pengamatan 24 dan > 48 jam.Hasil dan Kesimpulan : Ketokonazol berpengaruh terhadap pertumbuhan Candida albicans dengan membunuh pada konsentrasi 32 µg/ml dengan waktu pemaparan 1 x 24 jam dan bersifat menghambat pertumbuhannya pada konsentrasi 8 ug/ml dengan waktu pemaparan 1 x 24 jam. Flukonazol berpengaruh terhadap pertumbuhan Candida albicans dengan membunuh pada konsentrasi 12,8 µg/ml dengan waktu pemaparan 2 x 24 jam dan konsentrasi 6,4 ug/ml dengan waktu pemaparan 3 x 24 jam. Dengan demikian dapat disimpulkan bahwa pada penelitian ini ketokonazol bersifat menghambat dan membunuh pertumbuhan Candida albicans dan flukonazol bersifat membunuh pertumbuhannya."

"Xylitol is reported to inhibit the growth of C. albicans. Objectives: Investigating serum factor role in inhibiting the growth of C. albicans and the effect of 1%, 5%, 10% xylitol in C. albicans resistance in serum in vitro. Methods: Identification of C. albicans (oral swab of candidiasis patient) was conducted using CHROMAgar, confirmed by germ tube test. The cultures were serially diluted, inoculated in Saburoud Dextrose Broth (SDB) contained 0% (control), 1%, 5%, or 10% xylitol, and kept for 3 or 7 days. These inoculations were then exposed to either active or inactive serum (Fetal Bovine Serum heated in 65°C for 30 minutes) for 2 hours in 37°C. The colony forming unit (CFU) of C. albicans in Saburoud Dextrose Agar (SDA) were counted after 2 days. C. albicans ATCC 10231 strain was used as a comparison. One-way ANOVA with a 0.05 was used. results: After 3 days cultured in media with or without xylitol, the CFU of C. albicans exposed to active serum were significantly lower than those exposed to inactive serum (p=0.032). Although not statistically significant (p=0.689), increased concentration of xylitollead to increased resistance of C. albicans in active serum. Only 7 day exposure of 10% xylitol resulted in significantly higher growth of C. albicans (p=0.034). No significant difference of C. albicans CFU in active or inactive serum (p=0.404). Conclusion: Serum factor has role in inhibiting C. albicans growth in vitro. Exposure of 1%, 5%, or 10% xylitol for 3 or 7 days has no significant effect on C. albicans resistance in serum."

"Pola makan modern kaya karbohidrat merupakan salah satu faktor predisposisi terjadinya kandidiasis oral. Namun belum jelas diketahui apakah pertumbuhan C. albicans akan meningkat bila terjadi glukosa dalam medium pertumbuhan. Tujuan: Menganalisis efek penambahan glukosa 1%, 5%, 10% terhadap pertumbuhan C. albicans in vitro. Metode: Isolat C. albicans klinik dari usapan mukosa mulut pasien kandidiasis oral dideteksi pada CHROMagar dan serum. Sebagai pembanding, C. albicans strain ATCC 10231 juga dideteksi dengan cara yang sama. C. albicans yang tumbuh dibiak dalam SDA selama 2 hari, kemudian dikumpulkan dan dibiakkan kembali dalam SDB yang telah ditambah glukosa 1%, 5%, dan 10% selama 3 atau 7 hari pada suhu ruang. Sebagai kontrol adalah C. albicans yang ditumbuhkan dalam SDB tanpa penambahan glukosa. Pertumbuhan C. albicans diukur dengan menghitung CFU/ml C. albicans dalam cawan petri. Uji statistik menggunaka ANOVA dengan a 0.05. Hasil: Setelah 3 hari, pertumbuhan C. albicans isolat klinik 1%, 5%, dan 10% berturut-turut adalah 181.5, 582, dan 811 CFU/ml; sedangkan C. albicans ATCC 10231 adalah 21.5, 177.5, 375.5 CFU/ml. Pertumbuhan tersebut lebih rendah dibandingkan kelompok kontrol yaitu 970 (isolat klinik) dan 957 (ATCC) CFU/ml. Setelah 7 hari diperoleh pertumbuhan C. albicans isolat klinik adalah 2350, 9650, dan 9650 CFU/ml; sedangkan C. albicans ATCC 10231 adalah 5000, 5450, 3550 CFU/ml. Pertumbuhan kelompok kontrol 7 hari adalah 5000 (klinik) dan 5150 (ATCC) CFU/ml. Analisis ANOVA menunjukkan bahwa setelah 3 hari penambahan glukosa 1% menurunkan pertumbuhan C. albicans secara bermakna baik pada isolat klinik maupun strain ATCC 10231 (p < 0,05). Pada kelompok 7 hari penambahan glukosa 5% dan 10% meningkatkan pertumbuhan C. albicans isolat klinik secara bermakna (p < 0,05). Simpulan: Glukosa 5% dan 10% dapat meningkatkan pertumbuhan C. albicans in vitro. Penambahan glukosa 1% dapat menghambat pertumbuhan C. albicans pada durasi 3 hari.

---

High carbohydrate intake is one predisposing factor of oral andidiasis. Whether glucose addition in medium will increase the growth of Candida albicans is still unclear. Objective: Investigating the effect of 1%, 5%, 10% glucose addition on the growth of C.albicans in vitro. Methods: C. albicans sample was from oral swab of a male oral candidiasis patient. Detection of C. albicans used CHROMagar and confirmed by germ tube test. C. albicans colonies were inoculated in Sabouraud Dextrose Agar (SDA). As a comparison, C. albicans ATCC 10231 was also detected inthe same way. After 2 days the cultures were serially diluted and inoculated in Sabouraud Dextrose Broth (SDB) without glucose (control), 1%, 5%, or 10% additional glucose, kept for 3 or 7 days in room temperature, then inoculated in SDA. The Colony Forming Unit (CFU) were counted after 2 days. ANOVA with a 0.05 was used. Results: After 3 days, additional 1%, 5%, 10% glucose in media with clinical strain of C. albicans resulted in 181.5, 582, 811 CFU/ml respectively while in media with C. albicans ATCC were 21.5, 177.5, 375.5 CFU/ml. The growth of controls C. albicans were 970 (clinical strain) and 957 CFU/ml (ATCC). After 7 days, the growth of clinical strain of C. albicans with additional glucose 1%, 5%, 10% were 2350, 9650, 9650 CFU/ml respectively while the growth of C. albicans ATCC were 5000, 5450, 3550 CFU/ml. The growth of 7 days controls were 5000 (clinical strain) and 5150 (ATCC) CFU/ml. Statisticaly, additional 1% glucose for 3 days lead to significant decreased of growth of both clinical strain and ATCC 10231 C. albicans (p < 0,05). Additional 5% and 10% glucose for 7 days increased the growth of C.albicans significantly (p < 0,05). Conclusion: Additional 5% and 10% glucose for 7 days increase the growth of C. albicans in vitro. While additional 1% glucose for 3 days decrease the growth of C. albicans."

"Latar belakang: Propolis memiliki efek antifungal. Universitas Indonesia sedang mengembangkan permen mengandung propolis yang diduga dapat menghambat pertumbuhan C.albicans. Tujuan: Penelitian ini bertujuan untuk mengetahui efek ekstrak propolis dan permen propolis terhadap pertumbuhan C.albicans. Metode: Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) ekstrak propolis diuji menggunakan metode spektrofotometri serta jumlah koloni C.albicans pasca pemaparan ekstrak dan permen propolis dihitung secara langsung. Hasil: Nilai KHM dicapai pada konsentrasi 10%, nilai KBM dicapai pada konsentrasi 15%; serta terdapat penurunan jumlah koloni jamur pasca pemaparan ekstrak dan permen propolis. Kesimpulan: Ekstrak dan permen propolis terbukti efektif menghambat pertumbuhan C.albicans.

---

Background: Propolis has antifungal effect. Universitas Indonesia has been developing propolis candy which can inhibit C.albicans growth.

Objective: This research was aimed to analyze effect of propolis extract and candies to C.albicans growth.

Methods: After C.albicans were exposed to propolis extract and candies, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by spectrophotometry and post-exposure colonies of C.albicans were counted.

Result: MIC of propolis extract against C.albicans were determined at 10% and MBC at 15%, also the amount of C.albicans colonies were decreased after propolis extract and candies exposure.

Conclusion : Propolis extract and candies were effective to inhibit C.albicans growth."

"ABSTRAKLatar Belakang: Early Childhood Caries (ECC) merupakan adanya satu atau lebih gigi berlubang, hilang, atau ditambal pada anak anak dengan usia sampai dengan 71 bulan. Mikroorganisme utama dari karies adalah Streptococcus mutans yang terklasifikasi menjadi empat, yaitu serotipe c, e, f, dan k. Menurut penelitian sebelumnya, ditemukan banyak Candida albicans pada plak anak dengan ECC, namun interaksinya dengan Streptococcus mutans belum diketahui secara pasti. Tujuan: Menganalisis kuantitas dan hubungan dari antigen Streptococcus mutans serotipe e dengan Candida albicans pada plak anak dengan karies dini serta bebas karies dikaitkan dengan laju alir saliva. Metode: Kuantitas antigen dari 36 sampel plak karies dan 14 sampel bebas karies diketahui melalui uji ELISA kemudian dikaitkan dengan laju alir saliva. Hasil: Perbandingan antara kuantitas kedua antigen pada laju alir saliva <30 detik didapatkan nilai 0,000 dan pada laju alir 30-60 detik sebesar 0,001. Hubungan antara kuantitas Streptococcus mutans serotipe e dan Candida albicans pada plak karies didapatkan nilai r = 0,639 dan r = 0,247 untuk plak bebas karies. Kesimpulan: Terdapat perbedaan bermakna antara kuantitas kedua antigen pada masing-masing tingkat laju alir saliva dan terdapat korelasi positif antara kuantitas antigen Streptococcus mutans serotipe e dengan Candida albicans pada plak karies dan plak bebas karies. "

"Jamur Candida merupakan penyebab infeksi paling banyak ditemukan pada manusia. Spesies paling sering menyebabkan kandidiasis yaitu Candida albicans. Saat ini, insidensi infeksi kandidiasis semakin meningkat. Candida adalah penyebab utama keempat infeksi darah di rumah sakit, dan di Amerika Serikat, angka kematian akibat kandidemia mencapai 40 per tahun. Tujuan penelitian ini untuk mengetahui pola kepekaan Candida albicans dan Candida non albicans terhadap vorikonazol secara in vitro dari rekam medis 2010-2015 di Laboratorium Mikologi Departemen Parasitologi FKUI. Penelitian ini menggunakan studi cross-sectional. Pemilihan sampel menggunakan metode total sampling, data diproses menggunakan SPSS dan dianalisis menggunakan uji Fisher. Dari 546 sampel, hasil uji kepekaan Candida albicans terhadap vorikonazol menunjukkan 407 isolat sensitif 99,8 dan 1 isolat resisten 0,2. Uji kepekaan Candida non albicans terhadap vorikonazol menunjukkan 136 isolat sensitif 98,6 dan 2 isolat resisten 1,4 . Tidak terdapat perbedaan p=0,159 pola kepekaan Candida albicans dan Candida non albicans terhadap vorikonazol. Vorikonazol memilki aktivitas yang tinggi di dalam in vitro sehingga memberikan hasil yang baik untuk mengeradikasi Candida yang resisten terhadap flukonazol. Sebagai kesimpulan, tidak terdapat perbedaan pola kepekaan Candida albicans dan Candida non albicans terhadap vorikonazol.

---

Candida is the cause of most infections found in humans, mostly Candia albicans. Candida is the fourth leading cause of blood infection in hospitals, and in the United States, the death rate from Candidaemia reached 40 in year. The aim of this research is to determine the Candida albicans and Candida non albicans susceptibility profile in vitro to voriconazoleof the medical record 2010 2015 at the Mycology Laboratory of the Departement of Parasitology Faculty of Medicine University of Indonesia. This study uses a Cross sectional study. The sample selection was done with total sampling method. Data was processed using SPSS and analyzed using Fisher's test. From 546 samples, the susceptibility profile of Candida albicans are 407 samples 99.8 sensitive and 1 sample 0.2 resistant. Susceptibility profile of Candida non albicans are 136 samples 98.6 sensitive and 2 sample 1.4 resistant. The result indicated no significant association p 0.159 between susceptibility profile of Candida albicans and Candida non albicans to voriconazole. Voriconazole has high in vitro activities so as to provide good results to eradicate the Candida resistant to fluconazole. In conclusion, there are no significant association between Candida albicans and Candida non albicans susceptibility profile to voriconazole."

"Pembentukan biofilm dan aktivitas enzim proteinase merupakan faktor virulensi utama dari Candida albicansdalam menyebabkan infeksi oportunistik.Temulawak mengandung zat aktif xanthorrhizolyang bersifat antifungal.Tujuan: Menganalisis pengaruh eradikasi biofilmC. albicans fase awal, menengah dan maturasi oleh ekstrak etanol temulawak terhadap aktivitas enzim proteinase C. albicans ATCC 10231. Metode: Pemaparan ekstrak etanol temulawak pada biofilm C. albicans berbagai fase biofilm dan dilanjutkan uji aktivitas enzim proteinase. Hasil: Zona aktivitas enzim proteinase C. albicans pada kelompok uji yang telah dipaparkan Kadar Eradikasi Biofilm Minimal (KEBM) pada ekstrak etanol temulawak memiliki aktivitas lebih sedkit dibandingkan kontrol negatif pada semua fase dan setara dengan Nystatin.Kesimpulan: Eradikasi berbagai fase Biofilm C. albicansoleh ekstrak etanol temulawak sejalan dengan penurunan aktivitas enzim proteinase.Latar belakang: Pembentukan biofilm dan aktivitas enzim proteinase merupakan faktor virulensi utama dari Candida albicansdalam menyebabkan infeksi oportunistik.Temulawak mengandung zat aktif xanthorrhizolyang bersifat antifungal.Tujuan: Menganalisis pengaruh eradikasi biofilmC. albicans fase awal, menengah dan maturasi oleh ekstrak etanol temulawak terhadap aktivitas enzim proteinase C. albicans ATCC 10231. Metode: Pemaparan ekstrak etanol temulawak pada biofilm C. albicans berbagai fase biofilm dan dilanjutkan uji aktivitas enzim proteinase. Hasil: Zona aktivitas enzim proteinase C. albicans pada kelompok uji yang telah dipaparkan Kadar Eradikasi Biofilm Minimal (KEBM) pada ekstrak etanol temulawak memiliki aktivitas lebih sedkit dibandingkan kontrol negatif pada semua fase dan setara dengan Nystatin.Kesimpulan: Eradikasi berbagai fase Biofilm C. albicansoleh ekstrak etanol temulawak sejalan dengan penurunan aktivitas enzim proteinase.

---

The formation of biofilm and activity of proteinase enzymes are the main virulence factors of Candida albicans in causing opportunistic infections. Javanese turmeric contains an active substance xanthorrhizol that had been reported to have antifungal effect. Objective: To analyze the effect of Candida albicans biofilm eradication on the initial, intermediate and maturation phase by Javanese turmeric ethanol extract to the proteinase enzyme activity of C. albicans ATCC 10231. Methods: The Exposure of Javanese turmeric ethanol extract on Candida albicans biofilm in any phases and followed by the proteinase enzyme activity assay. Results: Proteinase enzym activity zone of C. Albicans on test group that had been exposed with Minimum Bactericidal Concentration (MBC) of Javanese Turmeric Ethanol Extract has less enzyme activity than negative controls and equivalent to Nystatin. Conclusion: Eradication on any phase of C. albicans by Temulawak is in accordance with decrased proteinase enzyme activity."

"High carbohydrate intake is one of predisposing factors of oral candidiasis. Wheather glucose addition in medium will increase the growth of Candida albicans in vitro is subject to further investigation. Objective: Investigating the effect of 1%, 5%, 10% glucose addition on the growth of C. albicans in vitro. Method: C. albicans sample was taken from oral swab of a male oral candidiasis patient. Identification of C. albicans was conducted using CHROMagar and confirmed by germ tube formation in serum. C. albicans colonies were inoculated in SDB. As a comparison, C. albicans ATCC 10231 was used. After 2 days the cultures were serially diluted and inoculated in SDB without glucose (control), and with 1%, 5%, 10% addditional glucose, kept for 3 and 7 days in room temperature, then inoculated in SDA. The CFU/ml were counted after 2 days. ANOVA with α 0.05 was used. Result: After 3 days, additional 1%, 5%, and 10% glucose in media with clinical strain of C. albicans resulted in 181.5, 582, and 811 CFU/ml respectively while in media with C. albicans ATCC were 21.5, 177.5, 375.5 CFU/ml. The growth of C. albicans with no additional glucose were 970 (clinical strain) and 957 CFU/ml (ATCC). After 7 days, the growth of clinicalstrain of C. albicans with additional glucose 1%, 5%, 10% were 2350, 9650, 9560 CFU/ml respectively while the growth of C. albicans ATCC were 5000, 5450, 3550 CFU/ml. Statisticaly, additional 1% glucose for 3 days lead to significant decreased of growth of both clinical strain andATCC 10231 C. albicans (p < 0,05). However, only additional 5% and 10% glucose in clinical isolate for 7 days increased the growth of C. albicans significantly (p < 0,05). Conclusion: The effect of additional glucose on the increased growth of C. albicans in vitro is influenced by the concentration, exposure duration of glucose, and by the strain of C. albicans."