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"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL). Sebanyak 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA berturut-turut dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah dan uji LSD (P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan NaFeEDTA dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 3 di hari ke-21 yaitu 31,74% terhadap KK 1; dan 23,52% terhadap KK 2.

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The effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups. Normal control group (KK 1) which was administered with standard feeding and drinking only. Treatment control group (KK 2) which was administered with extra soymilk non fortificant, and three treatment groups which were administered with extra soymilk added with NaFeEDTA fortificant 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for 21 days consecutively. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post hoc LSD test (P < 0.05) showed significant effect of NaFeEDTA fortificant addition to soymilk on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 3 at t21 which is 31.74% to KK 1; and 23.52% to KK 2."

"Telah dilakukan penelitian yang bertujuan mengetahui pengaruh fortifikan NaFeEDTA dalam susu kedelai terhadap kadar hemoglobin darah tikus Rattus norvegicus L. jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap RAL . Sebanyak 25 ekor tikus putih jantan dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar tanpa pemberian susu kedelai dan fortifikan; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA dosis 2,7 mgFe/ kgBB; 5,4 mgFe/ kg BB; dan 10,8 mgFe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Pengukuran kadar hemoglobin darah tikus menggunakan hematology analyzer. Hasil uji ANAVA satu arah dan uji LSD P < 0,05 menunjukkan bahwa setelah pemberian perlakuan selama 21 hari berturut-turut, terdapat perbedaan kadar hemoglobin yang nyata antara seluruh kelompok perlakuan KP 1, KP 2 dan KP 3 terhadap kadar hemoglobin KK 1 dan perbedaan kadar hemoglobin yang nyata antara KP 2 dan KP 3 terhadap kadar hemoglobin KK 2. Peningkatan kadar hemoglobin tertinggi terjadi pada KP 2, yaitu 10,84 terhadap KK 1; dan 9,28 terhadap KK 2.

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The effect of NaFeEDTA fortificant in soymilk on blood hemoglobin levels of male Sprague Dawley rats Rattus norvegicus L. had been studied. By using Complete Random Design CRD , twenty five rats were divided into five groups. Normal control group KK 1 was administered with standard feeding and drinking without adding soymilk and fortificant. Treatment control group KK 2 was administered with soymilk without fortificant, and three treatment groups which were administered with soymilk added with NaFeEDTA fortificant 2.7 mg Fe kgbw KP 1 5.4 mg Fe kgbw KP 2 and 10.8 mg Fe kgbw KP 3 . All of the five groups were treated for 21 days consecutively. Measurement of rat blood hemoglobin levels uses the hematology analyzer. One way ANOVA test and post hoc LSD test P 0.05 showed that after 21 days of consecutive treatment, there was a significant effect on blood hemoglobin levels in all treatment groups KP 1, KP 2, and KP 3 compared to KP 1 and a significant effect on blood hemoglobin levels in KP 2 and KP 3 compared to KK 2. The highest increase of blood hemoglobin levels was detected on KP 2 which is 10.84 to KK 1 and 9.28 to KK 2."

"ABSTRAKTelah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberianfortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi plasma darahtikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitianmenggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putihjantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberilarutan CMC 0,5%; KK 2 yang diberi CMC 0,5% dan suspensi tepung tempetanpa fortifikan; dan KP 1, 2, dan 3 yang diberi CMC 0,5% dan tepung tempedengan fortifikan Fe Fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan padahari ke-0 dan setelah pencekokan hari ke-21. Darah ditentukan kadar zat besinyadengan AAS (Atomic Absorption Spectrophotometer). Hasil uji Anava satu arah(P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan Fe Fumarat dalamtepung tempe terhadap kadar zat besi antar kelompok perlakuan. Peningkatankadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 13,21% terhadap KK 1 dan11,48% terhadap KK 2.

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ABSTRACTThe effect of Fe fumarate fortificant addition in tempeh flour on plasma ironconcentration of male Sprague-Dawley rats (Rattus norvegicus L.) had beenstudied. By using Complete Random Design (CRD), twenty five rats were dividedinto five groups, consist of normal control group (KK 1) which was administeredwith CMC 0.5%, treatment control group (KK 2) which was administered withCMC 0.5% and tempeh flour non fortificant, and three treatment groups whichwere administered with tempeh flour added with Fe Fumarate fortificant 1.35 mgFe/KgBW (KP 1); 2.7 mg Fe/KgBW (KP 2); and 5.4 mg Fe/KgBW (KP 3). All ofthe five groups were treated within 21 consecutive days. The plasma ironconcentration was measured by Atomic Absorption Spectrophotometer (AAS).One way ANOVA test ( P < 0.05) showed significant effect of Fe Fumaratefortificant addition in tempeh flour intake on plasma iron concentration in alltreatment groups. The highest increase of plasma iron concentration was detectedon KP 1 which is 13.21% to KK 1 and 11.48% to KK 2."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi larutan CMC 0,5%; KK 2 yang diberi larutan CMC 0,5% dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi larutan CMC 0,5% dan susu kedelai dengan fortifikan Fe fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah pencekokan hari ke-21. Darah dipreparasi menggunakan destruksi basah lalu ditentukan kadar zat besinya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah (P < 0,05) menunjukkan pengaruh nyata pemberian fortifikan Fe fumarat dalam susu kedelai terhadap kadar zat besi antar kelompok perlakuan. Peningkatan kadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 27,90% terhadap KK 1 dan 17,49% terhadap KK 2.

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The effect of Fe fumarate fortificant addition in soy milk intake on plasma iron concentration of male Sprague-Dawley rats (Rattus norvegicus L.) had been studied. By using Complete Random Design (CRD), twenty five rats were divided into five groups, consist of normal control group (KK 1) which was administered with CMC 0.5% solution, treatment control group (KK 2) which was administered with CMC 0.5% solution and unfortified soy milk, and three treatment groups which were administered with soy milk added with fortificant Fe fumarate 1.35 mg Fe/kgbw (KP 1); 2.7 mg Fe/kgbw (KP 2); and 5.4 mg Fe/kgbw (KP 3). All of the five groups were treated for consecutive 21 days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANOVA test and post-hoc LSD test (P < 0.05) showed significant effect of fortificant Fe fumarate addition in soy milk intake on plasma iron concentration in all treatment groups. The highest increase of plasma iron concentration was detected on KP 1, which is 27.90% to KK 1 and 17.49% to KK 2."

"ABSTRAKTelah dilakukan penelitian untuk mengetahui pengaruh pemberian fortifikanNaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadar natrium plasmadarah tikus (Rattus norvegicus L.) jantan galur Sprague Dawley. Sebanyak 25ekor tikus dibagi ke dalam 5 kelompok: kelompok kontrol 1 (KK1) yang diberiCMC 0,5%, kelompok kontrol 2 (KK2) yang diberi tepung tempe atau susukedelai, dan tiga kelompok perlakuan (KP1, KP2, KP3) yang diberi tepung tempeatau susu kedelai dengan fortifikan NaFeEDTA dosis 1,35 mg Fe/ kgBB; 2,7 mgFe/ kgBB; 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Penentuan kadarnatrium plasma dengan alat AES (Atomic Emission Spectroscopy). Hasil ujiAnava satu arah (P > 0,05) menunjukkan tidak ada pengaruh nyata pemberianfortifikan NaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadarnatrium antar kelompok perlakuan. Kadar natrium plasma pada T21 dengan bahanuji tepung tempe dan susu kedelai tetap berada pada rentang normal antara 0,456mg/ml -- 0,586 mg/ml.

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ABSTRACTThe effect of NaFeEDTA fortificant inserted in tempeh flour and soy milk intakeon plasma sodium concentration in male Sprague-Dawley rats (Rattus norvegicusL.) had been studied. Twenty five rats were divided into five groups: controlgroup 1 (KK1) was administered with CMC 0.5%, control group 2 (KK2) wasadministered with tempeh flour or soy milk; three treatment groups (KP1, KP2,KP3) were administered with tempeh flour or soy milk added with fortificantNaFeEDTA 1.35 mg Fe/kgBw; 2.7 mg Fe/kgBw; 5.4 mg Fe/kgBw consecutivefor 21 days. Plasma sodium concentration was measured by AES (AtomicEmission Spectroscopy). One way Anova test (P > 0.05) showed there is nosignificant effect of fortificant NaFeEDTA inserted in tempeh flour and soy milkintake on plasma sodium concentration in all treatment groups. Plasma sodiumconcentration on T21 which was administered with tempeh flour and soy milkremains in normal range between 0.456 mg/ml ? 0.586 mg/ml."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh fortifikan NaFeEDTA dalam susu kedelai terhadap jumlah sel darah merah tikus Rattus norvegicus L. jantan galur Sprague-Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap RAL . Sebanyak 25 ekor tikus jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberi pakan dan minum standar tanpa pemberian susu kedelai dan fortifikan; KK 2 yang diberi pakan minum standar dan susu kedelai tanpa fortifikan; dan KP 1, 2, dan 3 yang diberi pakan minum standar dan susu kedelai dengan fortifikan NaFeEDTA dosis 2,7 mgFe/kgBB, 5,4 mgFe/kgBB, dan 10,8 mgFe/kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan pada hari ke-21. Pengukuran jumlah sel darah merah tikus menggunakan alat hematology analyzer. Hasil uji ANAVA satu arah dan uji LSD P le; 0,05 menunjukkan bahwa setelah pemberian perlakuan selama 21 hari berturut-turut, terdapat perbedaan jumlah sel darah merah yang nyata antara seluruh kelompok perlakuan KP 1, KP 2, dan KP 3 terhadap jumlah sel darah merah KK 1 dan KK 2. Peningkatan jumlah sel darah merah tertinggi terjadi pada KP 2 di hari ke-21 yaitu 19,70 terhadap KK 1; dan 17,27 terhadap KK 2.

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The effect of fortificant NaFeEDTA in soy milk on red blood cell count of male Sprague Dawley rats Rattus norvegicus L. had been studied. By using Complete Random Design CRD, twenty five rats were divided into five groups. Normal control group KK 1 which was administered with standard feeding and drinking without the addition of soy milk and fortificant. Treatment control group KK 2 which was administered with extra soy milk without fortificant, and three treatment groups which were administered with extra soy milk added with NaFeEDTA fortificant 2.7 mg Fe kgbw KP 1 5.4 mg Fe kgbw KP 2 and 10.8 mg Fe kgbw KP3 . All of the five groups were treated for 21 days consecutively. Measurement of rat red blood cell count uses the hematology analyzer. One way ANOVA test and post hoc LSD test P le 0.05 showed that after 21 days of consecutive treatment, there was a significant effect on red blood cell count in all treatment groups KP 1, KP 2 and KP 3 compared to KK 1 and KK 2. The highest increase of red blood cell count was detected on KP 2 at t21 which is 19,70 to KK 1 and 17,27 to KK 2."

"Telah dilakukan penelitian untuk mengetahui pengaruh pemberian madu PS (Pollen Substitute) terhadap konsentrasi trigliserida plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Dua puluh empat tikus jantan dibagi dalam empat kelompok, yaitu kelompok kontrol normal (KK1) yang tidak diberi diet tinggi lemak, kelompok kontrol perlakuan (KK2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan akuades, dan dua kelompok perlakuan (KP1 dan KP2) yang diberi diet tinggi lemak, larutan propylthiouracil (PTU) 0,01%, dan madu PS dengan dosis masing-masing 10% dan 20% b/v. Bahan uji diberikan setiap hari selama 14 hari berturut-turut. Hasil uji anava satu faktor (P < 0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap konsentrasi trigliserida akhir pada semua kelompok perlakuan. Hasil uji LSD (P<0,05) menunjukkan penurunan konsentrasi trigliserida pada seluruh kelompok perlakuan (KP1 dan KP2). Konsentrasi trigliserida pada kelompok perlakuan yang mendekati kelompok kontrol normal dicapai oleh kelompok perlakuan dengan dosis 20% b/v.

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The research was done to observe the effect of honey PS (Pollen Substitute) on the concentration of triglyceride in blood plasma of male rat (Rattus norvegicus L.) Sprague-Dawley strain. Twenty-five male rats were divided into four groups, consisting of normal control group (KK1) that was not given a high fat diet, treatment control group (KK2) that was given a high fat diet, 0.01% propylthiouracil (PTU) solution, and two treatment groups (KP1 and KP2) that were given a high fat diet, 0.01% propylthiouracil (PTU) solution, and honey PS with dose of 10% and 20% w/v, respectively, for 14 consecutive days.

The result of anova test (P < 0.05) showed significant effect of honey PS toward triglyceride concentration in all treatment groups. LSD test (P < 0,05) showed that the decreased blood total cholesterol concentration occurred at both treatment groups (KP1 and KP2). The concentration of triglyceride in the treatment group that approached the normal control group achieved by the treatment group with dose of 20% w/v."

"[ABSTRAKTelah dilakukan penelitian untuk mengetahui kadar kreatinin plasma tikus putih (Rattus norvegicus L.) jantan galur Sprague-Dawley setelah pemberian infusa daun sukun (Artocarpus altilis). Sebanyak 30 ekor tikus dibagi menjadi 6 kelompok, yaitu 2 kelompok kontrol dan 4 kelompok perlakuan yang diinduksi CCl4 kemudian diberikan infusa daun sukun dengan dosis 1,35; 2,7; 5,4; dan 10,8 g/kg BB. Uji kualitatif pada infusa daun sukun menunjukkan bahwa infusa daun sukun memiliki aktivitas antioksidan serta mengandung senyawa alkaloid dan flavonoid. Infusa diberikan sebanyak empat kali dengan selang waktu 12 jam. Pengambilan darah dilakukan sebanyak tiga kali, yaitu sebelum perlakuan, 12 jam setelah induksi CCl4, dan satu jam setelah pemberian infusa terakhir. Analisis sampel darah dilakukan menggunakan metode kolorimetri. Induksi CCl4 berhasil meningkatkan kadar kreatinin plasma tikus di atas batas normal. Rerata kadar kreatinin plasma tikus setelah pemberian infusa terakhir yaitu KK1 (0,80 0,11); KK2 (1,44 0,21); KP1 (1,12 0,42); KP2 (0,76 0,40); KP3 (0,56 0,06); dan KP4 (0,76 0,17). Uji LSD (P<0,05) menunjukkan bahwa terdapat perbedaan bermakna antara KK2 dengan KK1, KP2, KP3, dan KP4. Hal tersebut menunjukkan bahwa pemberian infusa daun sukun dengan dosis 2,7; 5,4; dan 10,8 g/kg BB berpengaruh terhadap kadar kreatinin plasma tikus.ABSTRACTThe present study was aim to assess plasma creatinine levels of male albino Sprague-Dawley rats (Rattus norvegicus L.) after breadfruit leaf (Artocarpus altilis) infusion intake. Thirty male rats were devided into six groups, consisting of two control group and four treatment groups CCl4-induced and were given breadfruit leaves infusion at concentration dose of 1,35; 2,7; 5,4; and 10,8 g/kg body weight, respectively. Qualitative test of breadfruit leaves infusion showed that it has antioxidant activity and positively contains alkaloid and flavonoid. Breadfruit leaves infusion were given orally and administered four times, with an interval of twelve hours. Plasma creatinine levels were measured three times, before treatment; 12 hours after CCl4-induced; and 1 hour after the last breadfruit infusion intake using colorimetric method. Plasma creatinine levels was elevated above the upper limits of normal after CCl4-induced. Mean of plasma kreatinine levels of the last analysis: KK1 (0,80 0,11); KK2 (1,44 0,21); KP1 (1,12 0,42); KP2 (0,76 0,40); KP3 (0,56 0,06); and KP4 (0,76 0,17) mg/dl. Least significant diffrence (LSD) test (P<0,05) showed a significant effect of breadfruit leaves infusion at dose of 2,7; 5,4; and 10,8 g/kg bw on plasma creatinine levels of rats., The present study was aim to assess plasma creatinine levels of male albino Sprague-Dawley rats (Rattus norvegicus L.) after breadfruit leaf (Artocarpus altilis) infusion intake. Thirty male rats were devided into six groups, consisting of two control group and four treatment groups CCl4-induced and were given breadfruit leaves infusion at concentration dose of 1,35; 2,7; 5,4; and 10,8 g/kg body weight, respectively. Qualitative test of breadfruit leaves infusion showed that it has antioxidant activity and positively contains alkaloid and flavonoid. Breadfruit leaves infusion were given orally and administered four times, with an interval of twelve hours. Plasma creatinine levels were measured three times, before treatment; 12 hours after CCl4-induced; and 1 hour after the last breadfruit infusion intake using colorimetric method. Plasma creatinine levels was elevated above the upper limits of normal after CCl4-induced. Mean of plasma kreatinine levels of the last analysis: KK1 (0,80 0,11); KK2 (1,44 0,21); KP1 (1,12 0,42); KP2 (0,76 0,40); KP3 (0,56 0,06); and KP4 (0,76 0,17) mg/dl. Least significant diffrence (LSD) test (P<0,05) showed a significant effect of breadfruit leaves infusion at dose of 2,7; 5,4; and 10,8 g/kg bw on plasma creatinine levels of rats.]"

"ABSTRAKTelah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberianfortifikan NaFeEDTA dalam tepung tempe terhadap kadar zat besi plasma darah tikus(Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitianmenggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putihjantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu kelompok kontrol normal(KK1) yang hanya diberikan CMC 0,5%, kelompok kontrol perlakuan (KK2) yangdiberikan suspensi tepung tempe tanpa fortifikan dan kelompok perlakuan 1, 2, 3(KP1, KP2 dan KP3) yang diberikan suspensi tepung tempe dengan fortifikanNaFeEDTA dosis 1,35 mgFe/ kgBB, 2,7 mgFe/ kg BB, dan 5,4 mgFe/ kgBB.Pemberian bahan tersebut dilakukan secara oral selama 21 hari berturut- turut.Pengambilan darah dilakukan pada hari ke-0 dan setelah perlakuan hari ke-21. KadarFe diukur dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji anava satuarah dan LSD (P < 0,05) terhadap sampel menunjukkan terdapatnya perbedaan nyatapemberian fortifikan NaFeEDTA dalam tepung tempe terhadap kadar zat besi selama21 hari. Peningkatan kadar zat besi tertinggi akhir penelitian (t21) terjadi pada KP 3,yaitu sebesar 27,40% terhadap KK1 dan 24,38% terhadap KK2.

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ABSTRACTThe study has been conducted to know the effect of fortificant NaFeEDTAadministration on tempeh flour to the plasma iron concentration of male rats (Rattus norvegicus L.). Twenty five male rats were divided to five groups consisting of normal control group (KK1) which was administered with CMC 0,5%; treatment control group (KK2) which was administered with tempeh flour without fortificant; and three treatment groups which were administered with tempeh flour and fortificant NaFeEDTA with different doses; 1,35 mgFe/KgBw (KP 1); 2,7 mgFe/KgBw (KP 2); and 5,4 mgFe/KgBw (KP 3). Treatments were carried out orally within 21""consecutive days. Blood is tested before treatment (t0) and after 21 days of treatment (t21). The plasma iron concentrations were measured by Atomic Absorption Spectrophotometer (AAS). Result was statistically tested with SPSS. One-way anova test (P < 0,05) and post hoc LSD test (P <0,005) showed that adding fortificant NaFeEDTA is giving a differences iron concentrations at blood levels of rats from the first day until last day of treatments. Increased iron levels are highest in the KP3 at day 21, which increased 27.40 % compared with KK 1 and 24,38% compared with KK 2."

"Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberian fortifikan NaFeEDTA dalam tepung tahu terhadap kadar zat besi plasma darah tikus (Rattus norvegicus L.) jantan galur Sprague- Dawley. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putih jantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK1 yang diberi larutan CMC 0,5%; KK2 yang diberi CMC 0,5% dan suspensi tepung tahu tanpa fortifikan; dan KP1, KP 2, dan KP3 yang diberi CMC 0,5% dan tepung tahu dengan fortifikan NaFeEDTA dosis 1,35 mg Fe/ kgBB; 2,7 mg Fe/ kg BB; dan 5,4 mg Fe/ kgBB selama 21 hari. Pengambilan darah dilakukan pada hari ke-7, 14, dan 21. Darah ditentukan kadar Fe nya dengan AAS (Atomic Absorption Spectrophotometer). Hasil uji ANAVA satu arah (P < 0,05) menunjukkan pengaruh nyata pemberian fortifikan NaFeEDTA dalam tepung tahu terhadap kadar zat besi antar kelompok perlakuan. Hasil uji LSD (P < 0,05) menunjukkan bahwa terdapat perbedaan kadar zat besi (Fe) dalam darah yang nyata antara KK1 dengan semua kelompok perlakuan (KP1, KP2, dan KP3) dan KK2 dengan semua kelompok perlakuan pada t14. Hasil uji paired-samples T test (P < 0,05) menunjukkan terdapat perbedaan yang nyata kadar zat besi semua kelompok perlakuan pada t14 dan t21. Peningkatan kadar zat besi tertinggi terjadi pada KP3, yaitu sebesar 53,18 % di t14.

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The effect of fortificant NaFeEDTA inserted in tofu flour intake on plasma iron concentration in male Sprague-Dawley rats (Rattus norvegicus L.) has been studied. Twenty five rats were divided into five groups, consist of normal contol group (KK1) which was administered with CMC 0,5%, treatment control group (KK2) which was administered with CMC 0,5% and tofu flour non fortificant, and three treatment groups wich was adminestered with tofu flour added with fortificant NaFeEDTA 1,35 mg Fe/kgbw (KP1); 2,7 mg Fe/kgbw (KP2); and 5,4 mg Fe/kgbw (KP3). All of the five groups were treated for 21 consecutive days. The plasma iron concentration was measured by Atomic Absorption Spectrophotometer (AAS). One way ANAVA test ( P < 0,05) showed significant effect of fortificant NaFeEDTA inserted in tofu flour intake on plasma iron concentration in all treatment groups. LSD test (P<0,05) showed that the plasma iron concentration significantly different between KK1 towards all treatment groups and KK2 towards all treatment groups. Paired-samples T Test (P < 0,05) showed that the plasma iron concentration significantly different in all treatment groups on t14 and t­21. The higest increase of plasma iron concentration was detected on KP3 at t14, whic is 53,18 %."