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"This book will contain a series of solicited chapters that concern with the molecular machines required by viruses to perform various essential functions of virus life cycle. The first three chapters (introduction, molecular machines and virus architecture) introduce the reader to the best known molecular machines and to the structure of viruses. The remainder of the book will examine in detail various stages of the viral life cycle. Beginning with the viral entry into a host cell, the book takes the reader through replication of the genome, synthesis and assembly of viral structural components, genome packaging and maturation into an infectious virion. Each chapter will describe the components of the respective machine in molecular or atomic detail, genetic and biochemical analyses, and mechanism. "

"ABSTRACTDengue is an infectious disease caused by dengue virus. Dengue endemic region includes America, Western Pacific,Africa, East Mediterranian, and South East Asia including Indonesia. An early diagnostic system specific for Indonesiais needed to control dengue in Indonesia. In this research, cloning of Non Structural 1 (NS1) gene from dengue virustype 3 (Indonesian strain D3-1703) into pYES2/CT vector was performed. In the long run, NS1 recombinant proteinwill be expressed inSaccharomyces cerevisiaefor diagnostic materials. Polymerase Chain Reaction (PCR)amplification of NS1 gene fragments were done with optimal annealing temperature at 55 ºC. NS1 gene fragment andpYES2/CT were cut by Bam H I and Not I enzymes. The digested pYES2/CT was dephosphosrylated using Calf Intestine Alkaline Phospatase enzyme. Ligation with the vector:insertratio of 1:12 and 1:20 resulted in 6 and 5 recombinant colony candidates respectively. Restriction enzyme and PCR verifications showed that 5 recombinant plasmids contained NS1 gene. Sequencing of the first 600 bp of one recombinant plasmid was performed. The blastnanalysis showed that it had a 99% identity with dengue virus type 3 strain FW06. Finally, it was shown that NS1 clonewithin pYES2/CT was in the correct Open Reading Frame and ready to be expressed in S. cerevisiae."