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"Objective: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of aging and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer.

Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method.

Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p < 0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p < 0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p < 0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7.

Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression."

"Objective: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of aging and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer. Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method. Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p<0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p < 0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p < 0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression.;"

"Latar belakang: Penelitian mengenai manfaat kedelai dalam penyembuhan penyakit diabetes mellitus DM sudah banyak dilakukan, namun belum diketahui pengaruh ekstrak kedelai terhadap peran protein TERT sel - pankreas. Penelitian ini bertujuan untuk mengukur kemampuan ekstrak kedelai dalam meningkatkan ekspresi TERT sel - pankreas pada tikus diabetes melitus. Metode Penelitian: Eksperimental dengan Randomized block design. Enam puluh tikus putih jantan galur Sprague-Dawley dikelompokkan secara acak menjadi 6 kelompok: 1 tikus normal, 2 tikus DM diinduksi aloksan , 3 tikus DM glibenklamid, 4 tikus DM ekstrak kedelai 1 mg/kgBB/hari, 5 tikus DM ekstrak kedelai 5 mg/kgBB/hari, 6 tikus DM ekstrak kedelai 25 mg/kgBB/hari. Analisis statistik dilakukan dengan menggunakan SPSS 20. Variabel yang diukur yaitu glukosa darah puasa, ekspresi TERT dan jumlah sel - pankreas. Hasil: glukosa darah puasa pada perlakuan dengan ekstrak kedelai menurun secara bermakna p < 0,05 dibandingkan dengan tikus diabetes mellitus. Ekspresi TERT pada DM 25 mg/kgBB/hari lebih tinggi secara bermakna p < 0,05 dibanding tikus diabetes, jumlah sel ? pankreas pada tikus perlakuan ekstrak kedelai lebih tinggi secara bermakna p < 0,05 dibanding tikus diabetes. Kesimpulan: Ekstrak kedelai 1, 5 dan 25 mg/kgBB/hari dapat meningkatkan ekspresi TERT sel b pankreas pada tikus diabetes mellitus yang diinduksi aloksan.

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Background: Studies on the benefit of soybean as a treatment for diabetes mellitus DM have been largely performed however, the effect of soybean extracts on the role of TERT protein in pancreatic cells has not been known. The aimed of this study is to measure the capacity of soybean extracts in increasing the TERT expression of pancreatic cells in rats with diabetes mellitus.

Methods: It was an experimental study with randomized block design. Sixty white male Sprague Dawley rats were randomly categorized into 6 groups 1 normal rats 2 rats with DM induced by alloxan 3 rats with DM glibenclamide 4 rats with DM 1 mg kgBW day soybean extracts 5 rats with DM 5 mg kgBW day soybean extracts 6 rats with DM 25 mg kgBW day soybean extracts. Statistical analysis was performed using SPSS software program version 20.0. The measured variables included fasting blood glucose level, TERT expressions and the number of pancreatic cells.

Results: The fasting blood glucose level in rats treated with soybean extracts was reduced significantly p 0.05 compared to rats in diabetic control group. There was a significantly higher TERT expression in rats with DM 25 mg kgBW day soybean extracts p 0.05 compared to rats in diabetic control group moreover, the number of pancreatic cells was also significantly higher in rats treated with soybean extracts p 0.05 than the diabetic rats.

Conclusion: Soybean extracts of 1, 5 and 25 mg kgBW day can increase the TERT expression of pancreatic cells in rats with diabetes mellitus induced by alloxan."

"Although the most common cause of lung cancer is long-term exposure to tobacco smoke, the role of genetic factor for the cell defense mechanism, such as MnSOD, should also be considered. This study aims to analyze the expression and genotype of MnSOD in lung cancer cells of smoker patients. Samples were normal and lung cancer cells of patients operated in Persahabatan Hospital from May to December 2008, as well as lung cancer cells extracted from FFPE collection. Leukocyte cells of healthy smoker subjects were used as controls. The MnSOD mRNA expression was analyzed using Real Time RT-PCR and the specific activity using xantin oxidase inhibition assay. The genotyping was performed using PCR-RFLP.The result showed that the MnSOD specific activity in lung cancer of smoker patients is higher than in leukocyte cells of smoker controls. Compared to the expression of MnSOD in the normal lung cells of patients, in the lung cancer cells the level of MnSOD mRNA was lower, whereas its specific activity was higher (1.988 times). The samples from lung cancer patients have a Val/Val genotype frequency of 100%. In this study, we could conclude that MnSOD expression is altered in lung cancer cells."

"Radiasi merupakan terapi pilihan untuk kanker serviks stadium III B, namun permasalahan timbul karena adanya sifat radioresisten. Sel punca kanker SPK merupakan salah satu faktor yang diduga berkontribusi terhadap hal tersebut. SOX2 dan OCT4 merupakan faktor transkripsi yang mengekspresikan sifat-sifat SPK, yaitu mengontrol sifat pluripoten, self-renewal, berperan pada karsinogenesis, metastasis, resistensi terhadap terapi dan rekurensi tumor. Faktor apoptosis, DNA repair dan telomerase merupakan mekanisme yang berkaitan dengan radioresisten. Penelitian ini bertujuan untuk mempelajari hubungan antara SOX2 dan OCT4 sebagai penanda SPK terhadap respons terapi radiasi, serta kaitannya dengan faktor apoptosis caspase-3 , DNA repair Chk1 dan telomerase hTERT .Penelitian ini merupakan case control, terhadap 48 kasus karsinoma sel skuamosa serviks stadium III B yang telah menjalani terapi radiasi/kemoradiasi di RS Cipto Mangunkusumo/FKUI. Kasus dibagi dalam 2 kelompok, yaitu hasil terapi komplet 27 kasus dan hasil terapi inkomplet 21 kasus . Kasus dengan respons awal terapi radiasi baik dilakukan pemeriksaan bulan Pap smear dan HPV pada bulan ke-6 atau sampai ke-12 setelah terapi. Ekspresi SOX2, OCT4, caspase-3, Chk1 dan hTERT diperiksa secara imunohistokimia dari blok parafin biopsi awal.Ekspresi kuat SOX2 dan OCT4 dengan H-score masing-masing lebih dari 96,6 dan 61,9 mempunyai hubungan bermakna dengan respons awal terapi radiasi maupun respons akhir terapi radiasi SOX2 p = 0,017, p = 0,004 dan OCT4 p < 0,001, p < 0,001 . Ditemukan hubungan bermakna antara ekspresi Chk1 dan hTERT dengan respons awal terapi radiasi Chk1 p = 0,006, hTERT p = 0,029 . Tidak ditemukan hubungan yang bermakna antara ekspresi caspase-3, Chk1, hTERT dengan ekspresi SOX2 dan OCT4. Uji multivariat menunjukkan bahwa SOX2 dan OCT4 yang paling memengaruhi respons terapi OR = 5,12, p = 0,040 dan OR = 17,03, p < 0,001, secara berurutan . Uji probabilitas menunjukkan kemungkinan respons akhir terapi radiasi inkomplet sebesar 87,91 bila ekspresi kedua penanda SPK kuat.Ekspresi kuat SOX2 dan OCT4 dapat memprediksi hasil terapi radiasi inkomplet pada karsinoma serviks stadium III B.

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Radiotherapy is the main choice of treatment for stage III B cervical cancer, but radioresistance becomes a difficult matter. Cancer stem cell is one of the factors suspected involving in radioresistant cancers. SOX2 and OCT4 are transcription factors which have pluripotent cell characteristics, and self renewal ability. They also involved in carcinogenesis, metastasis, tumor recurrent, and resistance toward therapy. Apoptotic, DNA repair, and telomerase factors are mechanisms that also contribute to radioresistance. This study aims to know the role of SOX2 and OCT4 as CSC markers, apoptotic factor caspase 3 , DNA repair Chk1 and telomerase hTERT toward radiotherapy.The design of this study was case control with 48 cases of stage III B cervical squamous cell carcinoma patients who had finished receiving radiation chemo radiation therapy at Cipto Mangunkusumo Hospital FMUI, Jakarta. They were classified in 2 groups based on the final response of treatment, which were complete and incomplete one. Pap smear and DNA HPV were performed in month 6 or until month 12 after therapy for good initial therapy. Immunohistochemistry was done to analyze SOX2, OCT4, caspase 3, Chk1 and hTERT expression from the paraffin block of initial biopsy.Strong expression of SOX2 and OCT4 with each H score was higher than 96.6, and 61.9 had significant association with both initial and final therapy response SOX2 p 0.017, p 0.004 and OCT4 p 0.001, p 0.001, repectively . There was significant association between expression of Chk1 and hTERT, and initial therapy response p 0.006 for Chk1, and p 0.029 for hTERT . No significant differences were found between caspase 3, Chk1, hTERT, and SOX2 and OCT4. Multivariate analysis showed SOX2 and OCT4 were the most influenced antibodies for radiotherapy response OR 5.12, p 0.040, and OR 17.03, p 0.001, respectively . The likelihood of incomplete final therapy response was 87.91 if the expression both of CSC markers were strong.Expression of SOX2, and OCT4 could predict the incomplete radiotherapy of stage III B cervical cancer cases. "

"Hipoksia merupakan salah satu faktor penyulit dalam pemberian terapi radiasi, di mana kondisi kekurangan oksigen pada jaringan tumor dapat mengurangi sensitivitasnya terhadap radiasi. Di sisi lain, saat ini perkembangan imunoterapi dalam terapi kanker sangatlah pesat, termasuk blokade immune checkpoint PD-L1, yang dianggap menjadi harapan baru bagi terapi kanker. Ekspresi PD-L1 telah diketahui meningkat setelah pemberian radiasi, sehingga dapat menjadi dasar pemberian imunoterapi dalam kombinasi dengan radiasi. Regulasi PD-L1 ini terutama diatur melalui jalur-jalur transduksi sinyal perbaikan DNA. Dalam kondisi hipoksia, belum banyak diketahui bagaimana respon PD-L1 pada sel kanker dengan atau tanpa radiasi. Dikaitkan dengan jalur perbaikan DNA, telah banyak studi yang meneliti pengaruh hipoksia terhadap jalur-jalur ini. Namun, belum ditelaah atau diteliti secara langsung pengaruh ini terhadap regulasi ekspresi PD-L1 pada sel. Studi ini merupakan studi eksplorasi awal pada bidang ini yang bertujuan untuk meneliti ekspresi PD-L1 pada kultur sel beberapa cell lines kanker (U2OS, A549, DU145, OE21) secara in vitro dengan perlakuan radiasi sinar X (5, 10, atau 20 Gy) dalam kondisi inkubasi hipoksia atau dalam perlakuan hipoksia saja selama 2, 24, 48, atau 72 jam. Data awal ini juga dilengkapi dengan analisis bioinformatika menggunakan data The Cancer Genome Atlas (TCGA) yang memperlihatkan perbedaan ekspresi PD-L1 pada peningkatan ekspresi HIF1α pada dataset yang hipoksik dan yang tidak. Pada seluruh cell lines yang diteliti, tidak tampak peningkatan ekspresi PD-L1 dalam inkubasi hipoksia (dengan kadar oksigen 0,5%, 0,1%, dan <0,1%) dengan ataupun tanpa radiasi X-ray. Analisis in silico menunjukkan bahwa korelasi positif antara kadar mRNA PD-L1 dan marker-marker hipoksia tampak lebih menonjol pada dataset yang tidak hipoksik dibandingkan yang paling hipoksik. Selanjutnya, perbedaan kadar HIF1A menunjukkan perbedaan kadar ekspresi PD-L1 yang signifikan hanya pada dataset yang tidak hipoksik, terutama pada sampel tanpa mutasi gen-gen DNA repair yang diteliti. Temuan ini dapat menjadi argument bahwa HIF1A tidak selalu dapat meningkatkan ekspresi PD-L1, terutama pada tumor-tumor yang sangat hipoksik.

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Hypoxia is one of adverse clinical prognosis factors in patients receiving radiation treatment, where oxygen deprivation in tumor tissue is known to reduce its sensitivity to radiation. While in the field of cancer treatment, immunotherapy has been rapidly advancing, including the blockade of immune checkpoint PD-L1, eliciting new hope in the horizon. PD-L1 expression interestingly has been reported to increase after irradiation, which may become a rationale for combining radiation and immunotherapy. This upregulation of PD-L1 is mainly conducted via DNA repair pathways. However, in hypoxic condition, not much is know on how PD-L1 expressions in cancer cells respond, with or without irradiation. In view of many reports of hypoxic modulation of DNA repair pathways, there has been no study to date that analyzes or reports specifically on how this modulation impacts regulation of PD-L1 expression in cells. This study aims to serve as a pilot explorative study in this exploration, which is to analyze PD-L1 expression in cell cultures of several human cancer cell lines (U2OS, A549, DU145, OE21) in vitro in hypoxia incubation (2, 24, 48, or 72 hours) with or without X-ray irradiation (5, 10, or 20 Gy). This primary data was also completed with bioinformatics analysis using The Cancer Genome Atlas (TCGA) database, which showed difference in PD-L1 expression in samples with higher expression of HIF1α between hypoxic and non-hypoxic datasets. In all cell lines used, there was no upregulation of PD-L1 expression after hypoxia incubation (in oxygen levels 0,5%, 0,1%, and below 0,1%) with or without X-ray irradiation. Although in silico analysis of TCGA databases showed positive correlation of PD-L1 and hypoxia markers mRNA levels, these were seen more prominently in non-hypoxic datasets compared to the most-hypoxic datasets. Further, differences in HIF1A levels showed very significant difference in PD-L1 expression only in nonhypoxic datasets, especially in samples without mutation in DNA repair genes. These results may propose the argument that HIF1A does not always promote PD-L1 expression, especially in very hypoxic tumors."

"Kanker kolorektal adalah salah satu penyakit dengan jumlah penderita yang banyak di Indonesia. Metode deteksi dini kanker kolorektal merupakan hal yang penting karena dilaporkan bahwa diagnosa pada tahap awal dapat meningkatkan tingkat kesembuhan. Salah satu metode deteksi dini yang umum dilakukan adalah melalui skrining biomarker kanker kolorektal pada darah perifer (peripheral blood). Dalam darah perifer tersebut diketahui terdapat circulating tumor cells (CTCs) yang menandakan metastasis dan peripheral blood mononuclear cells (PBMCs) yang menandakan imunitas penderita kanker kolorektal. Biomarker CD45 (gen CD45) umum digunakan sebagai marker dalam metode seleksi negatif CTCs karena hanya diekspresikan pada PBMCs, akan tetapi telah dilaporkan bahwa CD45 juga diekspresikan pada microenvironment tumor kanker kolorektal, sehingga diperlukan penelitian lebih lanjut terkait ekspresi gen CD45 pada CTCs. Tujuan dari penelitian ini adalah untuk mendeteksi ekspresi gen CD45 pada CTCs dan PBMCs penderita kanker kolorektal dengan metode semi-kuantitatif RT-PCR dan direct immunofluorescence agar mengetahui potensinya sebagai biomarker deteksi kanker kolorektal. Delapan sampel darah perifer dari pasien kanker kolorektal RSUPN Dr. Cipto Mangunkusumo dikumpulkan, kemudian CTCs dan PBMCs diisolasi dari sampel darah tersebut. Hasil penelitian menunjukkan mRNA gen CD45 terdeteksi pada seluruh sampel CTCs dan PBMCs, serta ekspresi relatif lebih tinggi pada sampel PBMCs, sedangkan hasil deteksi protein gen CD45 hanya terdeteksi pada satu sampel PBMCs. Maka dapat disimpulkan bahwa mRNA gen CD45 terdeteksi baik pada CTCs maupun PBMCs sehingga berpotensi sebagai biomarker kanker kolorektal.

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Colorectal cancer is one of the cancers with a high prevalence in Indonesia. The method for early detection of colorectal cancer is important because it has been reported that diagnosis in the early stages of the disease can increase the cure rate. One of the common methods for early detection is through screening the colorectal cancer biomarkers in peripheral blood that indicates metastasis (CTCs), and the immunity (PBMCs) of colorectal cancer patients. The CD45 biomarker (CD45 gene) is commonly used in the negative selection method for CTCs due to its expression only in PBMCs. Nevertheless, it has been reported that CD45 is also expressed in the microenvironment of colorectal cancer tumors, therefore further research is requireded. The purpose of this study is to detect the expression of the CD45 gene in CTCs and PBMCs of colorectal cancer patients using semi-quantitative RT-PCR and direct immunofluorescence in order to determine its potential as a biomarker for the detection of colorectal cancer. Peripheral blood samples from 8 patients at RSUPN Cipto Mangunkusumo were collected and then the CTCs and PBMCs were isolated from those samples. The results showed that the CD45 gene’s mRNA was detected in all samples of both, with relatively higher expression in the PBMCs, while the CD45 gene’s protein was detected only in one PBMCs sample. It can conclude that the mRNA of the CD45 gene was detected in both CTCs and PBMCs so that it has the potential as a biomarker of colorectal cancer. "

"Kanker ovarium merupakan kanker paling mematikan ke-8 pada perempuan di dunia. Pasien kanker ovarium umumnya akan mengalami kemoresistensi, kekambuhan dan prognosis buruk setelah operasi sitoreduktif dan kemoterapi berbasis platinum. Hal tersebut berhubungan dengan peningkatan ekspresi Cancer Stem Cells (CSCs) CD44+/CD24-, RAD6, dan penurunan DDB2. Penelitian ini bertujuan untuk menganalisis hubungan ekspresi CSCs, RAD6 dan DDB2 dengan kemoresistensi kanker ovarium di jaringan kanker ovarium dan sirkulasi darah.Penelitian kohort ambispektif ini dilakukan di RSUP Cipto Mangunkusumo, RSUD Tarakan, RSUP Dharmais, dan RSUP Fatmawati pada Februari 2018–Februari 2022. Subjek adalah 64 orang pasien yang dibagi menjadi dua kelompok. Semua subjek menjalani operasi sitoreduktif dan pemeriksaan histopatologi. Kemoterapi diberikan sebanyak enam seri diikuti enam bulan observasi, kemudian ditentukan respons terapi dengan kriteria Response Criteria in Solid Tumors (RECIST). Uji imunohistokimia dilakukan langsung ke jaringan kanker ovarium (retrospektif) dan uji flowsitometri darah (prospektif) untuk menilai Ekspresi CSCs, RAD6 dan DDB2.Terdapat peningkatan Ekspresi CSCs, RAD6 serta penurunan bermakna ekspresi DDB2 (p < 0,05) di jaringan kanker ovarium kemoresisten, dan peningkatan bermakna Ekspresi CSCs, dan RAD6 yang bermakna (p < 0,05) di sirkulasi darah penderita kanker ovarium. Ekspresi DDB2 di uji imunohistokimia adalah protein dengan nilai AUC terbaik sedangkan di uji flowsitometri, CSCs memiliki nilai AUC terbaik. Disusun skor IHC-UNEDO (imunohistokimia) dan skor FCM- UNEDO (flowsitometri) untuk membantu memprediksi respons terapi.Penelitian ini menunjukkan bahwa terdapat peningkatan Ekspresi CSCs, RAD6 dan penurunan DDB2 di jaringan kanker ovarium, serta peningkatan Ekspresi CSCs di sirkulasi darah penderita kanker ovarium dan protein tersebut merupakan prediktor respons terapi kanker ovarium yang baik."

"Latar Belakang: Kanker kepala dan leher merupakan penyakit yang disebabkan oleh proliferasi sel tidak terkontrol yang terpicu oleh faktor genetik dan lingkungan. Telomerase Reverse Transcriptase (TERT) merupakan gen untuk menginstruksikan pembuatan telomerase yang mencegah terjadinya pemendekan telomer. Tujuan: Penelitian ini bertujuan untuk menganalisis distribusi polimorfisme gen TERT pada kanker kepala dan leher dan non-kanker kepala dan leher. Metode: 50 sampel kanker kepala dan leher sebagai kelompok kasus dan 50 sampel non-kanker kepala dan leher sebagai kelompok kontrol. TERT VNTR MNS16A dicampur dengan ddH2O, enzim polimerase dan template DNA, lalu dianalisis menggunakan teknik PCR-VNTR dilanjutkan dengan elektroforesis untuk dianalisis. Dilanjutkan dengan analisis statistik menggunakan uji Continuity Correction. Hasil: Genotip LL ditemukan lebih tinggi pada kanker kepala dan leher dan non-kanker kepala dan leher. Genotip dan alel polimorfik ditemukan lebih tinggi pada kanker kepala dan leher (100% dan 88%) daripada nonkanker kepala dan leher (82% dan 47%). Uji Continuity Correction antara kanker kepala dan leher dan non-kanker kepala dan leher menunjukkan tidak adanya perbedaan bermakna (p-value=0.242). Kesimpulan: Terdapat hubungan antara polimorfisme TERT VNTR MNS16A dan kanker kepala dan leher.

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Background: Head and neck cancer is a disease caused by uncontrolled cell proliferation triggered by genetic and environmental factors. Telomerase Reverse Transcriptase (TERT) is a gene to instruct the manufacture of telomerase which prevents telomere shortening. Objective: This study aimed to analyze the distribution of the TERT gene polymorphisms in head and neck cancer and non-head and neck cancer. Methods: 50 samples of head and neck cancer as the case group and 50 samples of non-head and neck cancer as the control group. TERT VNTR MNS16A was mixed with ddH2O, polymerase enzyme and DNA template, then analyzed using PCR-VNTR technique followed by electrophoresis for analysis. Followed by statistical analysis using the Continuity Correction test. Results: The LL genotype was found to be higher in head and neck cancer and non-head and neck cancer. Polymorphic genotypes and alleles were found to be higher in head and neck cancers (100% and 88%) than in non-head and neck cancers (82% and 47%). Continuity Correction test between head and neck cancer and non-head and neck cancer showed no significant difference (p-value=0.242). Conclusion: There is a relationship between the TERT VNTR MNS16A polymorphism and head and neck cancer."