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"ABSTRAKDevelopment of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs)Dueantem Thongphueak, Kosum ChansiriCenter of Excellence in Biosensors, Srinakharinwirot University, Bangkok 10110, Thailand.Thayat SriyapaiFaculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok 10110, Thailand.Supatra Areekit, Somchai Santiwatanakul, Piyada WangroongsarbABSTRAKThe detection of Campylobacter spp. in meat products was developed by using loop-mediated isothermal amplification (LAMP) combined with DNA-based bioassay methods, including a lateral-flow dipstick (LFD) and gold nano-DNA probe (AuNPs) assay. The LAMP primers were designed from the conserved nucleotide regions of Campylobacter spp. The analytical sensitivity of the LAMP-LFD and LAMP-AuNPs analysis was 360 fg/μl. The analytical specificity of LAMP-based assays showed no cross-reactions to Listeria monocytogenes, Salmonella Typhimurium, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter aerogenes, Serratia marcescens, Vibrio parahaemolyticus, Vibrio cholerae, Klebsiella oxytoca and Citrobacter diversus.The sensitivity, specificity and accuracy of both LAMP-LFD and LAMP-AuNPs for the detection of pre-enrichment cultures from raw chicken meat samples were 100%, 95% and 96.67%, respectively. Since the processing time of LAMP-based assays is 60-90 minutes, it is applicable as a point-of-care screening test for food safety and as a process control of Campylobacter spp. contamination."

"ABSTRACTThe objective of the present study is to investigate the influence of external magnetic field on unsteady incompressible flow of water based nanofluid through a successively expanding or contracting channel with porous walls. the basic governing equations with boundary conditions are non-dimensionalized using appropriate transformation to ordinary differential equations, which are then solved using power series with the help of Hermite-Padè approximation method. the instability of the flow is shown using bifurcation graph and the dominating singularity behavior numerically. the regular effects of the different governing physical parameters specifically Hartmann number, volume friction of nanoparticles, non-dimensional shear stress and permeation Reynolds number on velocity profiles are depicted graphically."

"ABSTRAKHigh annealing temperature - random amplified polymorphic DNA (HAT-RAPD) marker was used to identify and investigate the genetic relationship among 15 Paphiopedilum species of Venus slipper. The total of 72 primers was screened and 16 primers could be used for DNA amplification with clear amplified products to construct DNA fingerprints. The total of 248 polymorphic bands was found. A dendrogram, which constructed from the polymorphic bands using UPGMA by the NTSYS program, showed genetic similarities among 15 Paphiopedilum species with similarity coefficients ranging from 0.252 to 0.624. The orchids were classified into 3 clusters. These results indicated that the HAT-RAPD markers are capable to specify Paphiopedilum, and can be used in the breeding program and for genetic resource conservation in the future."

"ABSTRAKSubspace-based direction-finding methods assume that all sources manifold vectors are linearly independent. However, when this condition is not satisfied, the estimation methods will subsequently fail to identify the directions of the sources. This undesirable effect is referred to as a manifold ambiguity. In this paper, the presence of anifold ambiguity associated to a higher-order statistics-based array processing is investigated. By analyzing the geometrical shape of the corresponding array manifold, a class of ambiguous sets based on auniform partition of the effective manifold curve can be found. A general procedure is provided in order to model and categorize these ambiguities into the form of ambiguous generator sets."

"ABSTRAKPengupasan sabut kelapa dengan cara tradisioanal masih saja dilakukan orang terlebih di daerah-daerah pedesaan. Demikian halnya mesin-mesin pengupas sabut kelapa yang diharapkan akan mampu memberi solusi mengatasi adanya kelemahan pada alat yang digunakan dengan cara tradisional. Namun keberadaan mesin-mesin kian hari kian berkembang. Sehingga dilakukanlah suatu penelitian dengan melakukan rancang bangun terhadap mesin pengupas sabut kelapa yang baru dengan kapasitas besar dan semi kontinu. Mesin ini mengalami perubahan dan inovasi dari mesin-mesin yang sudah ada sebelumnya. Kegiatan penelitian diawali dengan melakukan perancangan terhadap mesin, kemudian hasil perancangan direalisasikan dalam bentuk pembuatan mesin dan akhirnya dilakukan uji kinerja mesin. Pengujian dilakukan terhadap tiga jenis kelapa yang keadaan kulitnya basah, sedang dan kering. Masin-masing buah kelapa yang diuiji sebanyak 10 buah. Pada hasil pengujian Waktu rata-rata pengupasan untuk tiap buah kelapa yang bersabut basah selama 13,1 detik per buah, untuk kelapa sedang 17,2 detik dan untuk kelapa kering selama 22 detik. Sedang kapasitas mesin adalah: untuk pengupasan jenis kelapa basah, kapasitas mesin adalah sebanyak 275 buah/jam, untuk kelapa sedang adalah sebanyak 209 buah/jam dan untuk kelapa kering sebanyak 163 buah/jam. Hasil kesimpulan dari penelitian adalah: analisis secara umum kelapa basah lebih mudah dikupas, sebab kelapa basah seratnya lebih banyak mengandung air sehingga gampang/lebih mudah dipisahkan dari kulitnya dibandingkan dengan kelapa yang lebih kering, seratnya hampir tidak mempunyai kadar air atau kondisi sabut kelapa kering."

"ABSTRAKThe objective of this study was to compare the anthraquinone content of cassod tree leaves, golden shower pods and noni roots that were determined in the form of free anthraquinone and anthraquinone glycoside by Soxhlet and decoction extraction method. The anthraquinone compounds were identified by the Borntrager reaction. The determination of anthraquinone UV-Visible spectrophotometry at 325nm was used. The results show that the highest anthraquinone content in the golden shower pods with 70% (v/v) ethanol was 193.79mg/g dry plant. The suitable solvent for the anthraquinone glycoside extracts was 80% (v/v) ethanol for the cassod tree leaves. In the testing of birds, 1% of the extracted golden shower pods that cover the seeds for food consumption showed a lower amount than that consumed in the control group (p <0.05)."

"ABSTRAKThis work analyzes heat integration of sugarcane wax extraction in purification plants. It focuses on the development of sugarcane wax extraction from the mud of sugar mill presses, using supercritical as a solvent and ethanol as a co-solvent, with further sugarcane wax purification of 25 kg per day. To improve heat recovery for the designed heat exchanger networks (HEN), pinch analysis was applied to achieve optimal, total, external hot and cold utility demands at the optimum minimum temperature difference of C. Generated HEN alternatives were selected based on an incremental net present value (NPV). Compared to the current HEN design, the most cost-effective HEN could decrease total annual costs by a factor of 1.2."

"ABSTRAKThis study conducts the energy and exergy analysis of a light-vent pipe which is integrated into attic space. The roof is a shed roof with 30 degrees of inclination angle. The LVP is manufactured from an aluminum sheet with a translucent flat cover and is 0.15 m in diameter. The results show that the test house integrated with the LVP could well transfer heat accumulated in attic space by natural ventilation through the LVP. The natural daylight has an influent to the air mass flow rate corresponding to energy and exergy efficiency. The total energy and exergy efficiencies were 37-67% and 12.3-33%, respectively. In addition, the ceiling heat gain and exergy heat gain performed that, the requirement of space cooling of the house with LVP lower than that the reference house."

"ABSTRAKCellular symptoms present a model system for the analysis of living structures in laboratories such as metabolic studies and drug screening. In living organisms, cells are often encountered with compaction, tension, and shear. Most cells are intangible and balanced when a solid or seminal stratification (monolayer culture) is connected. In distortion reaction, cells experience intense biochemical changes. The stressed cells on the culture combine in the Vivo environment, which creates dramatic shape changes and biochemical reactions. These microsystems can meet broad applications in the biomedical research field since deformation, compression or fluid flow have been found to induce biochemical changes in cells derived from various tissues. The aim of this project is to design and to develop a microfluidic device which will allow for the culturing of adhered cells in microfluidic chambers (micro-wells) while controlling, at the micro scale, the mechanical deformation applied on the substrate on which cells are attached. During this work, a Polydimethylsiloxane (PDMS) double layered microfluidic device was designed and fabricated which enables controlled micro-sized deformation of the cell culturing microwells. Moreover, a relation was found between measured deformation values and simulations."