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"Kanker serviks merupakan kanker ketiga tersering di seluruh dunia dengan angka kasus baru, morbiditas dan mortalitas yang tinggi. Tujuan dari penelitian ini adalah untuk mengetahui proporsi kesintasan lima tahun pasca radioterapi pasien KSS serviks stadium IIB-IIIB dan hubungannya dengan infeksi HPV serta faktor lain yang mempengaruhi. Penelitian ini merupakan penelitian kohort. Populasi terjangkau adalah pasien karsinoma serviks stadium IIB dan IIIB dengan hasil biopsi serviks KSS yang telah menjalani radioterapi di RSCM dan dilakukan pemeriksan DNA HPV pre dan pasca radiasi pada penelitian terdahulu. Analisis statistik digunakan dengan uji prognostik Kaplan Meier. Dari 31 sampel penelitian pendahuluan, hanya 27 subjek yang dapat didata. Angka kesintasan lima tahun adalah sebesar 35,5%. Tidak didapatkan hubungan yang bermakna antara kesintasan dengan infeksi HPV, infeksi HPV yang menetap, lama radiasi, LVSI, stadium, diferensiasi, ukuran tumor dengan masing-masing nilai p 0,921, 0,586, 0,718, 0,65, 0,139, 0,78, dan 0,139. Terdapat hubungan yang bermakna antara respon radiasi dengan kesintasan, dengan median time survival 2 tahun (p 0,016).

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Cervical cancer is the third most common cancer in the world with high number of new cases, morbidity and mortality rates. The objective of this research is to know the proportion of five year survival rate after radiation of cervical cancer stage IIB-IIIB patient and its relationship with HPV infection and other influencing factors. This research method was cohort study. Research population was patients with biopsy result squamous cell carcinoma stage IIB-IIIB who underwent radiation therapy and have been examined for HPV DNA before and after radiation on previous study. Overall survival was assessed and the relationship between prognosis with HPV infection and other factors was calculated. Statistical analysis was calculated using Kaplan Meier to determine prognostic factors of cervical cancer, as well as the median survival rate. From 31 samples on previous study, only 27 patients has been documented. The five year overall survival rate was 35,5%. There were no statistically significant relationship between cervical cancer survival rate with HPV infection, HPV persistence after radiation, duration of radiation, LVSI, staging, grading, tumor size with p result 0,921, 0,586, 0,718, 0,65, 0,139, 0,78, and 0,139 respectively. There was significant relationship between radiation response and survival rate with median 2-year survival (p 0,016)"

"Latar Belakang: Kanker serviks masih menjadi kanker tersering kedua di Indonesia dengan insiden 2638 kasus pada tahun 2008. Sejak tahun 1999, National Cancer Institute merekomendasikan penatalaksanaan kanker serviks dengan menggunakan kemoradiasi. Namun selama ini tatalaksana kanker serviks lebih banyak mengacu untuk klasifikasi histopatologi karsinoma sel skuamosa, sementara angka kanker serviks dengan tipe sel adenokarsinoma meningkat dan menurut beberapa penelitian memiliki prognosis yang lebih buruk. Tujuan: Penelitian ini memiliki tujuan mengetahui perbandingan keberhasilan terapi dengan kemoradiasi dibandingkan dengan radiasi saja serta untuk mengetahui adanya perbedaan respon terapi pada kanker serviks dengan klasifikasi histopatologi karsinoma sel skuamosa dan adenokarsinoma. Metode: yang digunakan adalah secara cross sectional dimana sampel diambil dari pasien kanker serviks IIB ndash; IIIB pada tahun 2011 ndash; 2013 di RSCM yang menjalani terapi radiasi atau kemoradiasi. Hasil: Dari 163 sampel yang dipelajari, sebanyak 107 pasien adalah pasien dengan karsinoma sel skuamosa dan 56 pasien dengan adenokarsinoma. Menurut klasifikasi histopatologi, karsinoma sel skuamosa didapatkan memiliki angka respon lengkap yang tidak berbeda secara signifikan, yaitu 82,2 dibandingkan dengan adenokarsinoma 78,6 p = 0,721 Sebanyak 67,5 pasien mendapatkan terapi radiasi dan 32,5 mendapatkan terapi kemoradiasi dengan agen kemoterapi berbasis platinum, terapi dengan kemoradiasi didapatkan memiliki respon terapi yang lebih baik yaitu dengan angka respon lengkap sebanyak 98,1 dibandingkan dengan radiasi 72,7 p = 0,001 . Pada kelompok pasien yang dilakukan radiasi, pasien dengan karsinoma sel skuamosa tidak memiliki respon yang berbeda dengan kemoradiasi, yaitu respon komplit 76,9 dibandingkan dengan 62,5 p= 0,191 . Begitu juga dengan kelompok kemoradiasi, tidak ada perbedaan respon terapi antara pasien karsinoma sel skuamosa 96,6 dengan adenokarsinoma 100. Kesimpulan: Dari penelitian ini didapatkan tidak ada perbedaan respon terapi pada tipe sel karsinoma sel skuamosa dan adenokarsinoma. Pada masing-masing kelompok radiasi dan kemoradiasi, karsinoma sel skuamosa dan adenokarsinoma tidak terdapat perbedaan. Pasien yang diterapi dengan kemoradiasi memiliki respon terapi yang lebih baik dibandingkan radiasi saja.

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Background: Cervical cancer is the second most frequent cancer in Indonesia with incident of 2638 cases in 2008. Since 1999, National Cancer Institute in the United States recommend to give concurrent chemoradiation for advanced stage cervical cancer. Until now the therapy recommention mostly addressed for squamous cell carcinoma, meanwhile the incidence of adenocarcinoma arise with also worse prognosis.

Objective: To know the correlation between histopathological type squamous cell carcinoma and adenocarcinoma with the teurapeutic response and to compare the response of treatment using radiation only and chemoradiation.

Method: This study was using cross sectional method, sampel was taken by secondary data using medical report of patient with cervical cancer staged IIB IIIB year 2011 ndash 2013 at RSCM who underwent radiation and chemoradiation.

Result: From 163 subjects, 107 was patient with squamous cell carcinoma and 56 patients with adenocarcinoma. According to histopathological type, squamous cell carcinoma and adenocarcinoma had insignificant difference in theurapetical response, which is 82,2, compared to 78,6 p 0,721. There were 67,5 patients got radiation only and 32,5 got concurrent chemoradiation therapy using platinum based agent. Among patient who were treated with chemoradiation, 98,1 patients achieved complete respons and for patient with radiation only 72,7 achieved complete respons 72,7 p 0,001. Patients who were treated with radiation only, when compared to its pathological type, the complete respons were not different 76,9 in squamous cell carcinoma, compared to 62,5 in adenocarcinoma p 0,191. And so as patient with chemoradiation, there were no difference in theurapetical respons in squamous cell carcinoma 96,6 compared with 100 in adenocarcinoma.

Conclusion: There was no difference in theurapetical respons in patient with squamous cell carcinoma compared to adenocarcinoma. Chemoradiation appeared to have better theurapetic respon compared to radiation only therapy. In each theurapetic modality group the respon therapy for squamous cell carcinoma and adenocarcinoma had no difference in theurapetical response."

"Latar belakang: Kanker serviks merupakan kanker terbanyak ketiga diderita oleh perempuan dengan morbiditas dan mortalitas tinggi. Tatalaksana kanker serviks stadium lanjut dengan radiasi baik itu radiasi saja maupun kemoradiasi. Overall survival (OS)dan disease free survival (DFS) pada kanker serviks stadium lanjut dengan histologi karsinoma sel skuamosa (KSS) dan adenokarsinoma (AK) berbeda pada beberapa penelitian. Begitu juga dengan terjadinya kekambuhanTujuan: (1) Mengetahui OS pada jenis histologi KSS dan AK kanker serviks stadium lanjut (2) Mengetahui DFS kanker serviks stadium lanjutMetode: Penelitian ini menggunakan studi kohort retrospektif menggunakan data rekam medis pasien kanker serviks stadium IIB hingga IVA dengan histologi KSS dan AK pada tahun 2008 hingga 2013. Pengamatan dilakukan saat subjek pertama kali didiagnosis kanker ovarium hingga terjadi peristiwa hidup, meninggal, atau hilang dari pengamatan dalam waktu 120 bulan.Hasil: Dari 518 pasien yang memenuhi kriteria, 426 pasien dengan jenis histologi KSS, 92 pasien dengan jenis AK. Hasil dari uji log rank p value=0,07 menunjukkan tidak ada perbedaan bermakna OS KSS dan AK dengan probabilitas kumulatif 42% pada bulan ke 120 Uji chi square didapati perbedaan bermakna p=0,042 terjadinya relaps pada AK dan KSS sbesar 26,1% dan 16,4%.Kesimpulan: Tidak ada perbedaan bermakna antara karsinoma sel skuamosa dan adenokarsinoma secara overall survival pada kanker serviks stadium lanjut dan terdapat perbedaan bermakna disease free survival pada kedua jenis histologi tersebut.

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Background: Cervical cancer is the third most common cancer among women with high morbidity and mortality. Management of advanced stage cervical cancer with radiation be it radiation alone or chemoradiation. Overall survival (OS) and disease free survival (DFS) in advanced cervical cancer with histology of squamous cell carcinoma (KSS) and adenocarcinoma (AK) differ in several studies. Likewise with recurrence Objectives: (1) Knowing OS in the type of histology of SCC and AK in advanced cervical cancer (2) Knowing DFS in advanced cervical cancerMethod: This study used a retrospective cohort using data from medical records of stage IIB to IVA cervical cancer patients with histology of SCC and AK in 2008 to 2013. Observations were made when the subject was first diagnosed with ovarian cancer until a life event, death, or disappear from observation in time 120 monthsResults: Of the 518 patients who met the criteria, 426 patients with type of KSS histology, 92 patients with type AK. The results of the log rank test p value = 0.07 showed no significant difference in OS KSS and AK with a cumulative probability of 42% in the 120th month 16.4%.Conclusion: There was no significant difference OS between SCC and AC in advanced stage of cervical cancer and there were significant differences in disease free survival in the two types of histology"

"Latar Belakang: Kanker serviks merupakan keganasan yang sering ditemukan diberbagai negara pada wanita setelah kanker payudara. Kanker serviks berhubungan dengan angka kematian yang tinggi. FIGO merekomendasikan penggunaan MRI sebagai alat diagnosis dan prognosis. Tingkat proliferasi tumor berhubungan dengan respon terapi yang dapat diketahui dengan nilai signal intensitas sekuens T2WI. Saat ini belum ada penelitian yang menilai perbedaan nilai SI sekuens T2WI dengan respon terapi radiasi pada kanker serviks tipe karsinoma sel skuamosa. Tujuan: Memperoleh perbedaan nilai rasio sekuens T2WI pada pasien kanker serviks karsinoma sel skuamosa yang mengalami respon dan tidak respon terapi. Metode: Sebanyak 39 subjek penelitian dilakukan pemeriksaan MRI pelvis sebelum dan setelah terapi radiasi. Data penelitian diambil menggunakan sekuens T2WI dan data histologi berasal dari EHR RSUPN Dr. Cipto Mangunkusumo. Dilakukan analisis data menggunakan uji normalitas Saphiro-Wilk dan uji T berpasangan. Hasil: Pada kelompok umur, status pernikahan, status obstetri dan klasifikasi FIGO, didapatkan hasil tidak signifikan (p = 0,19, p = 0,348, p = 0,153, dan p = 0,995; p > 0,05). Begitupun pada kelompok respon dan tidak respon dengan RECIST 1.1, didapat hasil signifkan dengan p = 0,000; p < 0,05) sedangkan pada kelompok perbedaan nilai rasio sekuens T2WI, didapatkan hasil yang tidak signifikan (p = 0,436, p > 0,05). Kesimpulan: Tidak terdapat perbedaan nilai rasio sekuens T2WI pada kelompok respon dan tidak respon terapi berdasarkan kriteria RECIST 1.1 pada kanker serviks tipe karsinoma sel skuamosa.

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Background: Cervical cancer is a malignancy that is often found in various countries in women after breast cancer. Cervical cancer is associated with a high mortality rate. FIGO recommends the use of MRI as a diagnostic and prognostic tool. The rate of tumor proliferation is related to the therapeutic response which can be determined by the value of the T2WI sequence intensity signal. Currently, there are no studies that assess the differences in SI values of T2WI sequences and the response to radiation therapy in squamous cell carcinoma type cervical cancer.

Objective: Obtain differences in the value of the T2WI sequence ratio in patients with cervical cancer squamous cell carcinoma who experienced and did not respond to therapy.

Methods: A total of 39 study subjects were subjected to pelvic MRI examinations before and after radiation therapy. The research data were taken using T2WI sequences and histological data came from EHR RSUPN Dr. Cipto Mangunkusumo. Data were analyzed using the Saphiro-Wilk normality test and paired T test.

Results: In the age group, marital status, obstetric status and FIGO classification, the results were not significant (p = 0.19, p = 0.348, p = 0.153, and p = 0.995; p> 0.05). Likewise in the response dan unresponse group with RECIST 1.1, the results were significant with p = 0.000; p <0.05), while the difference in the value of the T2WI sequence ratio, the results were not significant (p = 0.436, p> 0.05).

Conclusion: There is no difference in the value of the T2WI sequence ratio in the response group and no response to therapy based on RECIST 1.1 criteria in squamous cell carcinoma type cervical cancer."

"ABSTRAKLatar Belakang: Kanker serviks stadium lanjut lokal (IIB-IIIB) masih menjadi beban kesehatan di Indonesia saat ini. Radiasi menjadi modalitas utama terapi pada stadium ini. Programmed Death-Ligand 1 (PD-L1) merupakan sebuah ligand yang diekspresikan pada sel tumor yang terkait dengan proses immune escape. Sampai saat ini belum diketahui karakteristik kadar PD-L1 pada karsinoma sel skuamosa (KSS) serviks stadium lanjut lokal serta pengaruh radiasi terhadap ekspresinya. Penelitian ini bertujuan untuk melihat karakteristik PD-L1 intratumoral pada kanker serviks stadium lanjut lokal serta pengaruh radiasi eksterna terhadap ekspresinya. Metode: Dilakukan pemeriksaan kadar PD-L1 pada sampel biopsi serviks dengan 2 metode, yaitu Enzyme Linked Immuno Sorbent Assay (ELISA) dan immunohistokimia (IHK). Pengambilan sampel dilakukan dua kali, yaitu preradiasi dan pascaradiasi eksterna. Dilakukan analisis statistik untuk mengetahui perbedaan kadar antara sebelum dan sesudah radiasi. Selain itu dilakukan analisis untuk melihat kesesuaian antara kadar yang ditunjukkan pada metode ELISA dengan metode IHK. Hasil: Didapatkan 29 sampel KSS serviks stadium lanjut lokal yang memenuhi kriteria inklusi dan eksklusi. Dari pemeriksaan IHK, didapatkan bahwa PD-L1 diekspresikan hampir pada seluruh subjek (96,5%). Didapatkan nilai median PD-L1 ELISA preradiasi 409,19 pg/mg protein (59,80-3011,30), pascaradiasi 444,40 pg/mg protein (27,24-3217,85). Tidak didapatkan perbedaan bermakna antara kedua kelompok tersebut (p = 0,804). Pada analisis receiver operating characteristics (ROC) didapatkan nilai ELISA >400 pg/mg protein bersifat prediktif menyebabkan terjadinya penurunan kadar ELISA pascaradiasi. Terdapat kesesuaian antara kadar PD-L1 metode ELISA dengan metode IHK, dimana nilai ELISA > 499 pg/mg protein cenderung menunjukkan nilai grade 3 pada pemeriksaan IHK. Kesimpulan: PD-L1 diekspresikan positif pada KSS serviks uteri stadium lanjut lokal. Tidak terlalu jelas efek radiasi dalam menyebabkan naik-turunnya ekspresi PD-L1. Pemeriksaan ELISA mempunyai potensi untuk dipertimbangkan mewakili hasil pemeriksaan IHK, namun perlu bukti yang lebih kuat berupa penelitian dengan jumlah sampel yang lebih banyak.

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ABSTRACTBackground: Locally advanced cervical cancer (IIB-IIIB) remains a health burden in Indonesia. Radiation is the main modality of therapy at this stage. PD-L1 is a ligand that is expressed in tumor cells associated with the immune escape process. Until now there is no clear characteristics of PD-L1 levels in locally advanced-stage cervical SCC and the effect of radiation on its expression. This study is aimed to look for the intratumoral PD-L1 characteristics in locally advanced cervical cancer and the effect of external radiation on its expression. Method: PD-L1 levels were examined on cervical biopsy samples using two methods, i.e. ELISA and IHC. Biopsy was carried out twice, preradiation and post-external radiation. Statistical analysis was performed to determine differences in levels between before and after radiation. In addition, an analysis was conducted to see the conformity between the levels indicated in the ELISA method and the IHC method. Results: Twenty nine samples of local advanced cervical SCC were obtained that met the inclusion and exclusion criteria. From the IHC examination, it was found that PD-L1 was expressed in almost all subjects (96.5%). The median PD-L1 concentration of ELISA PD-L1 preradiation was 409.19 pg / mg protein (59.80-3011.30), post-radiation 444.40 pg / mg protein (27.24-3217.85). No significant difference was found between the two groups (p = 0.804). In the ROC analysis it was found that ELISA values > 400 pg / mg protein were predictive to cause a decrease in postradiation ELISA levels. There is a conformality between the levels of PD-L1 ELISA method with the IHC method, where the ELISA value > 499 pg/mg of protein tends to show grade 3 values ​​on the IHC examination. Conclusion: PD-L1 was expressed positively in locally advanced cervical SCC. The effects of radiation in causing the ups and downs of the expression of PD-L1 is not very clear. ELISA examination has the potential to be considered as a representative to the results of the IHC examination, but stronger evidence is needed in the form of study with a larger number of samples."

"Latar belakang: Kanker serviks menduduki peringkat kedua sebagai kanker tersering di seluruh dunia. Karsinoma sel skuamosa KSS adalah jenis histopatologi kanker serviks tersering. Prognosis terapi dan kesintasan KSS serviks menjadi informasi yang penting untuk pasien dan klinisi.Tujuan: 1.Memperbaiki prognosis dan respon terapi radiasi pasien KSS serviks; 2.Mengetahui respon pasca terapi radiasi pada pasien KSS serviks stadium IIIB; 3.Mengetahui prevalensi dan deskripsi sosiodemografi pasien KSS serviks stadium IIIB; 4.Mengetahui perbedaan klinikopatologik pasien KSS serviks stadium IIIB ditinjau dari: diameter massa serviks, derajat diferensiasi sel kanker, dan jenis histopatologi sel kanker; Mengetahui kesintasan 1 tahun setelah menjalani terapi radiasi. Metode: Dilakukan telaah historical kohort pada 76 pasien KSS serviks stadium IIIB di RSCM dari tahun 2016-2017. Pasien dikelompokkan menjadi KSS serviks stadium IIIB dengan pembesaran KGB dan tanpa pembesaran KGB. Dilakukan analisis univariat, bivariat, dan multivariat. Kesintasan 1 tahun dianalisis dengan menggunakan Kaplan-Meier. Hasil: Prevalensi terbagi menjadi 36 pasien 47,4 dengan pembesaran KGB, dan 40 pasien 52,6 tanpa pembesaran KGB. Respon terapi radiasi pada KSS serviks IIIB dengan pembesaran KGB lebih rendah, RR 4,26 1,96 -9,27, IK 95 . Skor prediktor 2.1 sebagai titik potong untuk menentukan diagnosis respons negatif pada terapi radiasi LR 2,31, sensitivitas 96,3 , spesifisitas 58,3 , dan akurasi 77,3 . Deskripsi sosiodemografi dan klinikopatologi sebanding antara kedua kelompok. Kesintasan 1 tahun KSS serviks stadium IIIB tanpa pembesaran KGB lebih baik HR 9,57. Kesimpulan: Terdapat perbedaan bermakna terhadap respon terapi radiasi antara kelompok KSS serviks stadium IIIB dengan pembesaran KGB dan tanpa pembesaran KGB.

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Background: Cervical cancer is second rank as the most common cancer worldwide. Squamous cell carcinoma SCC is the most common histopathology type for cervical cancers. The radiotherapy prognostic and survival rate of cervical SCC becomes important information for patients and clinicians.

Objectives: 1 To improve prognostic and radiotherapy respond from SCC; 2 knowing the radiotherapy response in patients stage IIIB cervical SCC with lymph node enlargement LNE compared to without LNE; 3 knowing the prevalence and description of sociodemographic; 4 knowing clinicopathologic differences in stage IIIB cervical SCC patients in terms of: cervical mass diameter, differentiated grade of cancer cells, and histopathologic type of cancer cells. 5 Knowing 1 year survival rate after radiotherapy.

Method: A historical cohort study was undertaken in 76 patients with stage IIIB cervical SCC in RSCM from 2016-2017. Patients are grouped into stage IIIB cervical SCC with LNE and without LNE. Univariate, bivariate, and multivariate analyzes were performed. The 1-year survival was analyzed using Kaplan-Meier.

Results: Prevalence was divided into 36 patients 47.4 with LNE, and 40 patients 52.6 without LNE. The radiotherapy respon for stage IIIB cervical SCC with LNE is worst than without LNE, RR 4.26 1.96-9.27, 95 IK . Predictor score ge;2.1 as the cutoff point to determine negative response on radiotherapy LR 2.31, sensitivity 96.3 , specificity 58.3 , and accuracy 77.3 . Sociodemographic and clinicopathologic descriptions were comparable between the two groups. The 1-year survival of stage IIIB cervical SCC without LNE better than with LNE, HR 9.57 3.28 ndash;27.88 95 IK.

Conclusion: There was a significant difference to radiotherapy response between the stage IIIB cervical SCC with LNE and without LNE."

"Latar Belakang : Pasien kanker serviks di Indonesia terbanyak dalam stadium lanjut. Terapi standarnya adalah radiasi. Respons terapi radiasi tidak selalu komplet. MnSOD merupakan garda terdepan melawan radikal bebas yang dihasilkan radiasi. Aktifitas MnSOD dipikirkan dapat digunakan sebagai prediktor respons terapi radiasi. Diperkirakan semakin tinggi aktifitas MnSOD akan semakin buruk respons radiasinya. Tujuan : Menilai aktifitas MnSOD pada biopsi KSS serviks stadium IIIB sebagai prediktor keberhasilan terapi radiasi. Metode : Penelitian potong lintang komparatif ini dilakukan di Divisi Onkologi Ginekologi, Departemen Obstetri dan Ginekologi FKUI, RSCM Jakarta dan Departemen Biokimia dan Biologi Molekuler FKUI. Dilakukan penelusuran data base penelitian sebelumnya untuk mengindentifikasi sampel respons positif dan negatif, dilanjutkan pemeriksaan aktifitas MnSOD dengan sprektrofotometri metode McCord dan Fridovich menggunakan kit RanSOD . Data komparatif yang didapat kemudian di analisis. Hasil : Didapatkan 76 sampel yang memenuhi kriteria inklusi terdiri dari respons positif 47 61,8 negatif 29 38,2 . Dilakukan kategorisasi aktifitas MnSOD dengan titik potong pada nilai 13,126 U/mL. Dengan uji chi-square didapat hubungan bermakna secara statistik antara aktifitas MnSOD pada pasien kanker stadium IIIB dengan respons terapi radiasi. Nilai RR sebesar 1,849 1.075-3.178, IK 95 . Kesintasan dengan analisis bivariat memakai metode Kaplan-Meier: pasien dengan aktifitas MnSOD cutoff < 13,126 U/mL memiliki tingkat kesintasan 1 tahun yang lebih baik 63 dibandingkan dengan pasien kanker serviks IIIB dengan nilai aktifitas MnSOD ge; 14 . Risiko kematian dengan pengujian bivariat metode regresi cox: pasien dengan aktifitas MnSOD cutoff 13,126 U/mL memiliki risiko kematian 1,055 kali IK 95 : 1,003-1,110 dibanding pasien dengan aktifitas MnSOD dibawah nilai cutoff. Dari analisis multivariat terlihat aktifitas MnSOD semakin kuat sebagai prediktor respons terapi radiasi. Kesimpulan : Aktifitas MnSOD tinggi pada jaringan KSS serviks stadium IIIB menghasilkan respons negatif dari terapi radiasi.

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Background: Most of the cervical cancer patients in Indonesia came with advanced stage. Therefore, the choice of treatment is radiotherapy. Although, radiotherapy does not always result in complete response. MnSOD is considered to be one the antioxidant enzyme which has the ability to work against free radicals. Its activity is expected to be acted as response predictor to radiotherapy treatment. It is hypothesized that high MNSOD activity tend to predict poor response of radiotherapy on advanced cervical cancer patients.

Objective : To investigate MnSOD activity on cervical SCC stage IIIB as a predictor of radiotherapy response.

Methods : It is a comparative cross sectional study conducted in the Gynecology Oncology Division, Obstetrics and Gynecology Department, Dr. Cipto Mangunkusumo Hospital Faculty of Medicine, University of Indonesia. Samples were collected from the tissue bank and research database. They were identified and divided into having positive or negative response to radiotherapy. In vitro experiment was conducted to measure the activity of MnSOD. Manganese superoxide dismutase was isolated using McCord and Fridovich method using RanSOD and the activity was analyzed using spectrophotometry. Data was then analyzed using SPSS.20 for comparative study.

Results : Seventy six samples were included in the study 47 61.8 with positive response and 29 38.2 with negative response on radiotherapy. Samples were then divided into having MnSOD activity of 13.126 U mL or 13.126 U mL. Univariate analysis chi square showed that there was statistically significant correlation between MnSOD activity and radiotherapy response in patients with cervical SCC stage IIIB RR 1.849 95 CI 1.075 3.178 . Survival analysis on the first year showed that patients with MnSOD activity 13.126 U mL had better survival than patients with MnSOD activity 13.126 U mL 63 vs 14 , Kaplan Meier study . Hazard ratio for overall survival was 1.055 95 CI 1.003 ndash 1.110 for patients with MnSOD activity of 13.126 U mL. Multivariate analysis showed that MnSOD activity was a strong predictor of radiotherapy response in this study.

Conclusion : This in vitro study showed that high activity of MnSOD was associated with poor response of radiotherapy for patients with cervical squamous carcinoma stage IIIB. "

"Latar belakang: Infeksi Human Papilloma Virus (HPV) merupakan salah satu faktor etiologi KSS (Karsinoma Sel Skuamosa) kepala dan leher serta mempengaruhi hasil terapi. Hipoksia tumor menunjukkan respon terapi yang buruk pada sebagian besar keganasan, termasuk KSS. Studi ini bertujuan mengevaluasi karakteristik HPV dan hipoksia dalam KSS rongga mulut dan orofaringeal serta kaitannya terhadap respons radiasi.Metode: Penelitian eksperimental pada KSS rongga mulut dan orofaring yang telah menjalani terapi radiasi di Departemen Radioterapi antara Januari 2013 hingga Desember 2017.Hasil: Dari total 18 subjek, 44,4% memiliki infeksi HPV positif, 66,7% menunjukkan marker hipoksia positif. Hubungan infeksi HPV dan hipoksia dalam penelitian ini menunjukkan tidak ada hubungan yang signifikan (OR HPV = 2,0 (0,2-15,3), OR hipoksia positif = 0,6 (0,15-2,5), atau hipoksia negatif = 1,2 (0,6-2,3), p = 0,638). Dalam penelitian ini, enam pasien (33,3%) memiliki respon lengkap, tujuh pasien (38,8%) memiliki respon parsial, satu pasien memiliki respon stabil, dan empat pasien (22,2%) memiliki respon progresif terhadap radiasi. Tidak ada hubungan yang signifikan antara HPV + / hipoksia +, HPV + / hipoksia-, HPV- / hipoksia +, dan kelompok HPV- / hipoksia- terhadap tanggapan terapi (p = 0,514).Kesimpulan: Peningkatan insiden HPV dan hipoksia tumor yang tinggi pada pasien dengan rongga mulut dan kanker orofaringeal. Namun, tidak ada efek signifikan dari HPV dan tumor hipoksia terhadap respons radiasi. Hipoksia berhubungan dengan respon progresif.

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Background: Human Papilloma Virus (HPV) infection can lead to head and neck cancer and affect therapy outcomes. Hypoxic tumors are known to show poor therapy response in majority of malignancies, including Squamous Cell Carcinoma (SCC). This study aimed to show characteristics of HPV and hypoxia in oral cavity and oropharyngeal cancer and their association in radiation response.

Method: We conducted an experimental study on patients diagnosed with oral cavity cancer and oropharyngeal cancer who had undergone radiation therapy in Radiotherapy Department between January 2013 until December 2017.

Result: From a total of eighteen subjects, 44.4% had positive HPV infection, 66.7% had positive for hypoxia.. The association of HPV infection and hypoxia in this study showed no significant relationship (OR HPV = 2.0(0.2-15.3), OR positive hypoxia = 0.6(0.15-2.5), OR negative hypoxia = 1.2(0.6-2.3), p=0.638). In this study, six patients (33.3%) had complete response, seven patients (38.8%) had partial response, one patient (5.6%) had stable response and four patients (22.2%) were progressive respon to the radiation. There was also no statistically significant association between HPV+/hypoxia+, HPV+/hypoxia-, HPV-/hypoxia+, and HPV-/hypoxia- group against therapy responses (p=0.514). In this study all progressive response related with hypoxia status.

Conclusion: There were high incidences of HPV and tumor hypoxia in patients with oral cavity and oropharyngeal cancer. However, there was no significant effects of HPV and tumor hypoxia to final radiation response. Hypoxia related to progressive disease. "

"Latar Belakang: Pasien kanker serviks di Indonesia 70 % datang pada stadium lanjut, 45,2% dengan stadium IIIB dan radiasi menjadi terapi utama. Radioresistensi menjadi masalah utama saat ini, namun radiosensitizer yang tersedia memiliki efek toksisitas tinggi. Curcumin sebagai fitofarmaka bisa menjadi alternatif radiosensitizer dengan cara menekan aktivitas enzim antioksidan thioredoxin reductase-1 dan memicu terjadinya proses apoptosis. Tujuan: Menilai efikasi Biocurcumin dalam menekan aktifitas thioredoxin reductase-1 dan pengaruhnya terhadap indeks apoptosis Metode: Penelitian payung uji klinis ini dilakukan di Divisi Ginekologi Onkologi Departemen OBGYN FKUI serta Departemen Biokimia FKUI. Dilakukan penelusuran archived data jaringan dari penelitian uji klinis utama. Pemeriksaan aktifitas thioredoxin reductase-1 pre dan pasca radiasi dilakukan pada 18 sampel yang diberi terapi radiasi dan BCM-95 serta 19 sampel dengan radiasi dan plasebo. Metode McCord dan Fridovich dipakai menggunakan kit human TrxR1(Thioredoxin Reductase-1) Elabscience. Dilakukan analisis perbedaaan kadar thioredoxin reductase-1 sebelum dan sesudah radiasi serta hubungan antara perubahan kadar thioredoxin reductase-1 dengan indeks apoptosis pada kedua kelompok. Hasil: Terdapat perbedaan bermakna perubahan rerata kadar thioreduxin reductase-1 pada kelompok yang mendapat terapi radiasi dan Biocurcumin dibandingkan kelompok yang mendapat terapi radiasi dan plasebo, nilai p = 0,02 (p < 0,05). Namun, tidak terdapat hubungan yang bermakna antara perubahan rerata Indeks Apoptosis pada kedua kelompok (nilai p>0,05). Selain itu juga tidak ada perbedaan nilai median pada perubahan kadar thioredoxin reductase-1 maupun perbedaan nilai rerata pada perubahan Indeks Apoptosis sebelum dan sesudah radiasi diantara keduanya (p > 0,05). Kesimpulan: BCM-95 efektif menurunkan rerata kadar thioredoxin reductase-1, namun tidak terdapat perbedaan indeks apoptosis pada kedua kelompok.

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Background: Cervical cancer patients in Indonesia 70% come at an advanced stage, 45.2% with stage IIIB and radiation being the main therapy. Radioresistence is a major problem nowadays, but available radiosensitizers have a high toxicity effect. Curcumin as phytopharmaceuticals can be an alternative radiosensitizer by suppressing the activity of antioxidant enzyme thioredoxin reductase-1 and triggering the occurrence of apoptosis process. Objective: Assessing the efficacy of Biocurcumin in suppressing thioredoxin reductase-1 activity and its effect on apoptosis index Method: This clinical trial umbrella research was conducted in the Division of Oncology Gynaecology, OBGYN Department FMUI as well as the Department of Biochemistry FMUI. Archived network data from major clinical trial research. Examination of thioredoxin reductase-1 pre and post radiation activity was conducted on 18 samples given radiation therapy and BCM-95 and 19 samples with radiation and placebo. The McCord and Fridovich methods were used using the Human TrxR1(Thioredoxin Reductase-1) Elabscience kit. An analysis of the difference in thioredoxin reductase-1 levels before and after radiation and the relationship between changes in thioredoxin reductase-1 levels and apoptosis index in both groups. Results: There was a meaningful difference in the average level of thioreduxin reductase-1 in the group that received radiation therapy and Biocurcumin compared to the group that received radiation therapy and placebo, the value p = 0.02 (p < 0.05). However, there is no meaningful relationship between the changes in the average of Apoptosis Index in both groups (p>0.05). In addition, there was no difference in the median value in the change in thioredoxin reductase-1 levels or the difference in average value in the changes in the Apoptosis Index before and after radiation between the two (p > 0.05). Conclusion: BCM-95 effectively lowered average levels of thioredoxin reduxtase-1, but there was no difference in apoptosis index in both groups."

"Pendahuluan: Penelitian ini bertujuan untuk mengetahui hubungan antara jumlah CD4, infeksi HPV, faktor risiko, dan terjadinya lesi prakanker pada pasien terinfeksi HIV.Metode: Studi potong-lintang ini dilakukan di Rumah Sakit Umum Dr. Cipto Mangunkusumo, Indonesia. 80 subjek penelitian dikumpulkan dari bulan Juli-Oktober 2021. Data subjek penelitian dikumpulkan menggunakan kuesioner terstruktur, meliputi usia, pendidikan, paritas, inisiasi seksual dini, jumlah pasangan seksual, riwayat merokok, riwayat kontrasepsi oral, riwayat penyakit menular seksual, dan jumlah CD4 terendah. Pemeriksaan sitologi, kolposkopi, dan tes HPV-DNA dilakukan pada seluruh subjek penelitian, dan 11 subjek melakukan pemeriksaan lanjutan histopatologi karena ditemukan abnormalitas pada pemeriksaan awal. Penginputan dan analisis data dilakukan dengan menggunakan IBM SPSS versi 25. Analisis bivariat, perhitungan odd ratio dan p value dilakukan untuk mengidentifikasi jumlah CD4, HPV-DNA, dan faktor risiko yang terkait dengan lesi prankanker serviks.Hasil: Dari data penelitian didapatkan bahwa 81,2% memiliki jumlah CD4 yang baik, dengan rata-rata jumlah CD4 sebesar 437,05 sel/mm3. Sebagian besar subjek memiliki HPV-negatif; namun, terdapat 22,2% subjek yang diketahui HPV-positif, memiliki lesi prakanker. Penelitian kami juga menemukan bahwa jumlah CD4 (p=0,01, OR 7,625; CI 95% 1,744-33,331) dan HPV-DNA (p<0,01, OR 12,286; CI 95% 1,456–103,65) secara signifikan berhubungan dengan lesi prakanker serviks. Kami juga menemukan korelasi antara inisiasi seksual dini dan hasil sitologi (p=0,05, OR 6,4; CI 95% 1,1306–36,2292).Kesimpulan: Jumlah CD4 yang rendah dan HPV-DNA positif berhubungan dengan perkembangan lesi prakanker. Pasien terinfeksi HIV dengan jumlah CD4 rendah juga dikaitkan dengan hasil HPV-DNA positif. Inisiasi seksual dini, sebagai faktor risiko kanker serviks, diketahui meningkatkan hasil skrining yang tidak normal. Oleh karena itu, metode skrining co-testing direkomendasikan sebagai strategi untuk mencegah kanker serviks pada semua pasien terinfeksi HIV.

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Introduction: This study aimed to determine the association between CD4 count, human papillomavirus (HPV) infection, risk factors, and the occurrence of precancerous lesions among HIV-infected patients.

Methods: This cross-sectional study was conducted at the Dr. Cipto Mangunkusumo General Hospital, Indonesia. All samples were collected between July and October 2021, and 80 HIV-infected subjects were included in the study. All participant data were collected using a structured questionnaire, including age, education, parity, early sexual initiation, number of sexual partners, history of smoking, history of oral contraception, history of sexually transmitted diseases, and the lowest CD4 count. Cytological examination, Colposcopy, and HPV DNA tests were performed on all participants, and 11 subjects underwent biopsy due to abnormalities. Data entry and analysis were performed using IBM SPSS 25th version. Bivariate analysis was performed, and odds ratios and p-values were computed to identify the CD4 count, HPV DNA, and risk factors associated with histopathology results.

Results: Among the participants, 81.2% had a good CD4 count, with a mean CD4 count of 437.05 cells/mm3. Most of the subjects were HPV-negative; however, 22.2% of HPV-positive subjects had precancerous lesions based on histopathologic results. Our study found that CD4 count was correlated with precancerous lesions (p=0.01, OR 7.625; CI 95% 1.744-33.331) and HPV DNA was significantly associated with cervical precancerous lesions (p<0.01, OR 12.286; CI 95% 1.456–103.65). Another finding was the correlation between early sexual initiation and cytology results (p = 0.05, OR 6.4; CI 95% 1.1306–36.2292).

Conclusion: Low CD4 counts and HPV DNA positivity are associated with the development of precancerous lesions. HIV-infected patients with low CD4 counts were also associated with positive HPV DNA results. Early sexual initiation, as a risk factor for cervical cancer, was found to increase abnormal screening results. Therefore, co-testing screening methods are recommended as a strategy to prevent cervical cancer in all HIV-infected patients."