Hasil Pencarian  ::  Simpan CSV :: Kembali

"Latar Belakang: L-arginin merupakan asam amino semiesensial yang produksinya tidak mencukupi kebutuhan dalam kondisi stres oksidatif akibat inflamasi. L-arginin adalah satu-satunya substrat bagi enzim nitric oxide synthase (NOS) yang memproduksi nitric oxide (NO) yang dapat mengaktivasi focal adhesion kinase (FAK) pathwaydan memicu terjadinya proses migrasi sel. Tujuan: Mengetahui potensi media kultur asam amino L-arginin terhadap laju kecepatan migrasi hDPSCs. Metode: Evaluasi media kultur asam amino L-arginin konsentrasi 300, 400, 500 ¼mol/L, serta DMEM sebagai kontrol terhadap laju kecepatan migrasi hDPSCs menggunakan uji scratch assay menggunakan uji scratch assay yang dihitung dengan rumus laju kecepatan migrasi setelah 24 jam. Analisis statistic menggunakan Paired T-Test dan Oneway ANOVA dengan post hoc LSD. Hasil: Terdapat perbedaan bermakna potensi L-arginin 500 μmol/L dibandingkan konsentrasi 300 dan 400 μmol/L, serta kontrol. Kesimpulan: Media kultur asam amino L-arginin 500 ¼mol/L memiliki potensi laju kecepatan migrasi yang lebih baik dibandingkan konsetrasi 300, 400 ¼mol/L dan kontrol.

---

Background: L-arginine is semiessential amino acid which the production is insufficient under oxidative stress due to inflammation. L-arginine is the only substrate of nitric oxide synthase (NOS) enzyme that produces nitric oxide (NO) which activates focal adhesion kinase (FAK) pathway to stimulate cell migration.

Objective: To understand potential of L-arginine amino acid culture media towards speed rate of hDPSCs migration.

Methods: Evaluation of 300, 400, 500 ¼mol/L of L-arginin amino acid culture media and DMEM as control towars speed rate of hDPSCs migration using scratch assay and calculation of migration speed rate after 24 hours. Statistical analysis using Paired T-Test and Oneway ANOVA with post hoc LSD.

Results: Significant result was shown between 500 ¼mol/L of L-arginin amino acid culture media compared with 300 and 400 ¼mol/L concentration and control towards migration speed rate after 24 hours.

Conclusion: 500 ¼mol/L of L-arginin amino acid culture media has a better migration rate compared with lower concentrations and control."

"Latar Belakang: Pada kondisi inflamasi pulpa, terdapat penurunan dari jumlah protein dan asam amino. L-Arginine adalah asam amino semi-esensial karena berperan penting pada kondisi tertentu, gangguan imun berat dan luka bakar, yang membutuhkan asupan tambahan L-Arginine eksternal. Asam amino L-Arginine menjadi satu-satunya substrat sintesis nitric oxide (NO) dan poliamina berasal dari konversi L-Arginine menjadi ornithinen melalui arginase. NO dan poliamina merangsang proliferasi sel dan memiliki efek positif pada perkembangan melalui siklus sel. Tujuan: Mengetahui potensi asam amino L-Arginine terhadap proliferasi hDPSCs. Metode: Evaluasi asam amino L- Arginine konsentrasi 300, 400, 500 μmol/L, serta DMEM sebagai kontrol terhadap proliferasi hDPSCs menggunakan uji cell count setelah 24 jam. Analisis statistic menggunakan Oneway ANOVA dengan post hoc Bonferroni. Hasil: Terdapat perbedaan bermakna potensi L-Arginine 300,400 dan 500 μmol/L dibandingkan kontrol, dan L- Arginine 500 μmol/L memiliki rerata proliferasi hDPSCs paling tinggi sebesar 436.666 sel/ml. Kesimpulan: Asam amino L-Argininee memiliki potensi terhadap proliferasi hDPSCs dan proliferasi tertinggi pada asam amino L-Arginine konsentrasi 500 μmol/L.

---

Background: In the inflammatory condition of the pulp, there is a decrease in the amount of protein and amino acids. L-Arginine is a semi-essential amino acid because it plays an important role in certain conditions, severe immune disorders and burns, which require additional intake of external L-Arginine. The amino acid L-Arginine is the sole substrate for the synthesis of nitric oxide (NO) and polyamines derived from the conversion of L- Arginine to ornithine via arginase. NO and polyamines stimulate cell proliferation and have a positive effect on progression through the cell cycle. Objective: To determine the potential of L-Arginine amino acid on the proliferation of hDPSCs. Methods: Evaluation of L-Arginine amino acid with concentrations of 300, 400, 500 μmol/L, and DMEM as a control for hDPSCs proliferation using cell count test after 24 hours. Statistical analysis using Oneway ANOVA with Bonferroni post hoc. Results: There was a significant difference in the potency of L-Arginine 300,400 and 500 μmol/L compared to control, and L-Arginine 500 mol/L had the highest average proliferation of hDPSCs of 436.666 cells/ml. Conclusion: The amino acid L-arginine has the potential to proliferate hDPSCs and the highest concentration at 500 μmol/L."

"Latar Belakang: Fokus disinfeksi saluran akar telah berubah dari disinfeksi agresif menjadi seleksi protektif dalam prosedur regeneratif endodontik. Larutan irigasi sintetik yang digunakan hingga saat ini toksik terhadap sel punca pulpa, salah satunya yang memiliki kemampuan proliferasi dan transdiferensiasi tinggi adalah hDPSCs. Oleh sebab itu, penelitian terkait disinfeksi berbahan alami yang mampu mempertahankan viabilitas sel punca terus berkembang pesat. Salah satu larutan irigasi alami yang bersifat antimikrobial dan agen kelator adalah larutan cuka apel. Untuk menjadikannya obat herbal terstandar hingga fitofarmaka, perlu diidentifikasi kelompok senyawa kimia dan uji viabilitas hDPSCs.Tujuan: Menganalisis pengaruh larutan cuka apel berbagai konsentrasi terhadap viabilitas hDPSCsMetode: hDPSCs ditambahkan DMEM+FBS10% (kontrol negatif), EDTA 17% (kontrol positif), larutan cuka apel dengan konsentrasi 2,5%, 5%, dan 10% dengan enam kali pengulangan. Selanjutnya, persentase viabilitas hDPSCs didapat dari MTT assays melalui microplate reader dalam nilai absorbansi. Data kemudian diolah statistik melalui uji parametrik One-way ANOVA.Hasil: Nilai rerata viabilitas sel hDPSC pada semua kelompok perlakuan bernilai diatas 70% sehingga tidak toksik menurut standar ISO dengan rerata viabilitas tertinggi pada kelompok 2,5% dan terendah pada kelompok EDTA 17% diikuti kelompok 10%.Kesimpulan: Larutan cuka apel dapat diidentifikasi kelompok senyawa kimia dan nilai viabilitas sel paling tinggi pada konsentrasi 2,5%.

---

Background: Focus on root canals disinfection have shift from aggressive to protective selection in regenerative endodontic procedures. Synthetic root canals irrigation that had been used until now are toxic toward pulp stem cells, one of them, hDPSCs which have higher proliferation and transdifferentiation ability. Therefore, research on natural disinfection which maintain stem cell viability keep developing rapidly. One of the natural disinfection that has antimicrobial effect and chelating agent is apple cider vinegar. To standardized it as modern medicine, need to identify group of chemical compounds and analyzing the viability percentage of hDPSCs.

Objective: Analyze the impact of apple cider vinegar solution in various concentrations on viability of hDPSCs.

Methods: hDPSCs were given DMEM+FBS10% (negative control), 17% EDTA (positive control), apple cider vinegar solution in 2.5%, 5% and 10% concentrations with six repetitions. Percentage viability of hDPSCs were analyze from MTT assays with microplate reader in absorbance value. Then, data were proccessed statictically with parametric One-way ANOVA.

Results: The average viability of hDPSCs were above 70% which considered non-toxic according to ISO, with the highest cells viability in 2.5% and the lowest cells viability in 17% EDTA followed by 10% groups.

Conclusion: Apple cider vinegar solution’s chemical compounds can be identified with the highest cells viability were at 2.5%."

"Latar Belakang: Obat berbahan dasar alami di Indonesia harus terstandar, memiliki pembuktian keamanan dan khasiatnya secara ilmiah melalui uji preklinik. Uji yang dapat dilakukan untuk mengetahui keamanan suatu bahan yaitu uji sitotoksisitas. Tujuan: Mengetahui sitotoksisitas ekstrak asam jawa 2,5%, 5%, dan 10% terhadap human dental pulp stem cells. Metode: Identifikasi komponen senyawa kimia dengan uji fitokimia dan GC-MS. Uji sitotoksisitas dengan uji viabilitas diukur menggunakan MTT assay untuk mengetahui aktivitas selular. Pengukuran menggunakan microplate reader panjang gelombang 570 nm. Semakin tinggi nilai OD yang didapat, maka nilai viabilitas sel akan semakin tinggi. Sel ditanam pada 96 well dengan densitas 5x103 sel/well. Analisa data menggunakan Kruskal-Wallis dan Post Hoc Mann-Whitney. Hasil: Dari nilai median viabilitas sel, kelompok larutan ekstrak asam jawa 2,5% memiliki nilai viabilitas yang paling tinggi, sedangkan larutan ekstrak asam jawa 10% memiliki nilai viabilitas yang paling rendah. Tidak terdapat perbedaan yang bermakna antara kelompok larutan ekstrak asam jawa 2,5% dan 5%. Kesimpulan: Larutan ekstrak asam jawa dari desa babakan kecamatan Darmaga Kabupaten Bogor dapat diidentifikasi komponen senyawa flavonoid dan saponin dan memiliki komponen senyawa kimia yang berpotensi sebagai antibakteri, antiinflamasi, anti virus, antioksidan, larutan khelasi, dan solvent. Larutan ekstrak asam jawa 2,5% dan 5% tidak toksik terhadap hDPSCs.

---

Background: Medicines made from natural ingredients in Indonesia must be standardized, having proven safety and efficacy through preclinical trials. The safety test that can be carried out is cytotoxicity test. Objective: To determine the cytotoxicity of 2,5%,5% and 10% Tamarindus indica extract on hDPSCs. Methods: identification of components chemical compound groups was measured by phytochemical test and GC-MS. Cytotoxicity test with viability test was measured using the MTT assay to determine cellular activity. Measured using microplate reader with a wavelength of 570nm. The higher OD value obtained, the higher the cell viability value. Cells inserted in 96 wells with a density of 5x103 cells/well. Data analysis using Kruskal-Wallis and Post Hoc Mann-Whitney. Results: From the median cell viability value, 2,5% Tamarindus indicia extract solution group had the highest viability value, while 10% Tamarindus indica etract had the lowest viability value. There was no significant difference between the 2,5% and 5% Tamarindus indica extract solution groups. Conclusion: Tamarindus indica extract from Babakan village, Darmaga Bogor can be identified as components of flavonoids and saponins, has chemical components that have potential as antibacterial, antiinflammation, antiviral, antioksidan, chelation solution, and solvents. Tamarindus indica extract of 2,5% and 5% were not toxic to hDPSCs."

"Latar belakang: Inflamasi adalah respon imun untuk mempertahankan inang terhadap patogen atau kerusakan. Namun, inflamasi yang tidak diatur dapat menyebabkan banyak penyakit. Peradangan terutama dimediasi oleh faktor kekebalan bawaan, termasuk sitokin, kemokin, dan sel kekebalan bawaan. Sitokin adalah mediator peradangan. Lipopolisakarida (LPS) adalah stimulator pulpitis yang kuat yang telah ditemukan pada jaringan pulpa yang meradang, menyebabkan pelepasan sitokin inflamasi termasuk IL-6, TNF-α dan IL-1β. Eksosom muncul sebagai strategi terapi mutakhir untuk mengobati sistem kekebalan tubuh akibat inflamasi. Sebagian besar sel mengeluarkan eksosom, yang dapat memiliki aktivitas imunomodulator yang kuat, tergantung pada jenis sel tempat mereka berasal. Sel punca mesenkim Wharton’s Jelly (hWJ-MSC) yang berasal dari tali pusar telah disarankan untuk mengurangi peradangan, sebagian melalui pelepasan eksosom.Tujuan: Untuk menyelidiki efek eksosom Wharton's Jelly pada konsentrasi 0,5%, 1% dan 5% pada sel punca pulpa gigi manusia yang terpapar LPS (hDPSC) pada waktu pengamatan 24, 48 dan 72 jam (analisis ekspresi IL-6)Metode: Menghitung jumlah ekspresi IL-6 pada human dental pulp stem cell (hDPSCs) yang terpapar LPS yang telah dikultur terlebih dahulu kemudian diberikan eksosom Wharton’s jelly dengan masing-masing konsentrasi 0,5%, 1%, 5% dan dibandingkan dengan kelompok kontrol tanpa pemberian eksosom pada waktu pengamatan 24 jam, 48 jam, dan 72 jam dengan analisis ELISA. Data dianalisis menggunakan analisis varian (ANOVA) satu arah dengan uji lanjut LSD.Hasil : Terdapat penurunan ekspresi IL-6 pada human dental pulp stem cells (hDPSCs) yang dipapar LPS menggunakan eksosom Wharton's Jelly dengan konsentrasi 1% dan 5% pada waktu pengamatan 48 jam.Kesimpulan: Eksosom Wharton's Jelly dapat menurunkan ekspresi IL-6 pada HDPSC yang terpapar LPS

---

Background: Inflammation is an immune response that defends the host against pathogens or damage. However, unregulated inflammation can cause many diseases. Inflammation is primarily mediated by innate immune factors, including cytokines, chemokines, and innate immune cells. Cytokines are the mediators of inflammation. Lipopolysaccharide (LPS) is a potent stimulator of pulpitis that has been found in inflamed pulp tissue, causing the release of inflammatory cytokines including IL-6, TNF-α dan IL-1β. Exosomes are emerging as a state-of-the-art therapeutic strategy for treating an overactive immune system. Most cells secrete exosomes, which can have a potent immunomodulatory activity, depending on the type of cell from which they originate. Human Wharton’s jelly mesenchymal stem cells (hWJ-MSC) derived from the umbilical cord have been suggested to reduce inflamation, in part through the release of extracellular vesicle-like exosomes.

Objective: To Investigate the effect of Wharton's Jelly exosomes at concentrations of 0.5%, 1% and 5% on LPS exposed human dental pulp stem cell (hDPSCs) at observation times of 24, 48 and 72 hours (IL-6 expresion analysis)

Methods: Calculating the amount of IL-6 expression in lps exposed human dental pulp stem cells that have been cultured first and then given wharton jelly exosomes with each concentration of 0.5%, 1%, 5% and compared with the control group without exosome administration at observation time 24 hours 28 hours and 72 hours using ELISA analysis. Data were analyzed using a one‐way analysis of variance (ANOVA) with LSD’S post‐test.

Result : There is a decrease in IL-6 expression in lps exposed human dental pulp stem cells (hDPSCs) using Wharton's Jelly exosomes with a concentration of 1% and 5% at 48 hours observation time.

Conclusions: Wharton's Jelly Exosome decrese IL-6 expresion on LPS‐exposed HDPSCs."

"Latar Belakang: Bahan irigasi endodontik yang sering digunakan saat ini memiliki sifat toksik terhadap sel punca yang berperan penting pada perawatan endodontik regeneratif. Sehingga dibutuhkan pemilihan bahan irigasi alami yang tidak toksik, seperti ekstrak jeruk purut yang juga memiliki efek antibakteri terhadap bakteri gram positif. Tujuan: Mendapatkan perbandingan sitotoksisitas larutan ekstrak daun jeruk purut berbagai konsentrasi pada waktu observasi 24 jam terhadap hDPSCs. Metode: HDPSCs diisolasi dari gigi molar tiga, diberikan media perlakuan berupa larutan ekstrak daun jeruk purut dengan konsentrasi 2,5%, 5%, 10% dan 20%, NaOCl 2,5% dan DMEM sebagai kontrol. Pengamatan proliferasi hDPSCs dengan uji MTT. Hasil dibaca dengan microplate reader dengan panjang gelombang 570 nm. Nilai absorbansi larutan ekstrak daun jeruk purut 2,5%, 5%, 10%, 20% dan NaOCl 2,5% dihitung persentasinya dibandingkan dengan nilai absobansi kelompok kontrol menggunakan rumus perhitungan viabilitas sel. Hasil: Nilai rerata viabilitas sel kelompok larutan ekstrak daun jeruk purut diatas 90%, menandakan larutan ekstrak daun jeruk purut tidak toksik terhadap hDPSCs. Kesimpulan: Larutan ekstrak daun jeruk purut tidak memiliki efek sitotoksisitas terhadap hDPSCs, dengan nilai viabilitas sel paling tinggi pada konsentrasi 20%."

"Latar Belakang: Terjadinya regenerasi pada proses penyembuhan luka pulpa yang mengalami cedera akan menggantikan struktur dan fisiologis jaringan sama dengan aslinya. Proses ini dimulai dengan sel punca pulpa bermigrasi ke tempat cedera dan berfungsi. Ketika ada invasi bakteri, lingkungan pulpa terinflamasi melepaskan berbagai sinyal termasuk sinyal yang memicu migrasi sel punca pulpa. Pentingnya proses migrasi pada penyembuhan jaringan pulpa yang terinflamasi, maka pada penelitian ini mengamati perbedaan kemampuan migrasi pada hDPSCs normal dan terinflamasi lipopolisakarida (LPS) bakteri E. coli dengan waktu observasi 6 jam dan 24 jam. Tujuan: Mengetahui perbedaan kemampuan migrasi pada hDPSCs normal dan terinflamasi yang dilihat dari laju kecepatan migrasi dan lebar luka hDPSCs pada hDPSCs normal dibandingkan dengan hDPSCs terinflamasi dengan waktu observasi 6 jam dan 24 jam. Metode: Penelitian ini merupakan penelitian eksperimental laboratorik in vitro dengan pengamatan migrasi menggunakan metode scratch assay. Hasil: Terdapat perbedaan bermakna laju kecepatan migrasi antara hDPSCs normal dan terinflamasi pada waktu observasi 6 dan 24 jam (p<0.05). Terdapat perbedaan bermakna lebar luka hDPSCs normal dan inflamasi pada waktu observasi 6 dan 24 jam (p<0.05). Kesimpulan: Hasil penelitian ini menunjukkan pulpa tetap memiliki potensi alamiah dalam menginduksi migrasi pada kondisi terinflamasi LPS bakteri E. coli pada periode waktu 24 jam.

---

Background: Regeneration in the injured pulp wound healing process will replace its structure and tissue physiology to be the same as the original. It begins with hDPSCs migrating to the injured site and functioning. When there is a bacterial invasion, the inflamed pulp environment releases various signals stimulating hDPSCs migration. Due to the importance of the migration process in inflamed pulp tissue wound healing, this research observed the differences in migration capability of the normal and inflamed-with lipopolysaccharide (LPS) bacteria E. coli- hDPSCs. Objective: To discover the differences in migration capability between normal and inflamed hDPSCs observed from differences in migratory speed rate and wound width of normal and inflamed hDPSCs at 6 and 24 hours observation time. Methods: This research was an experimental laboratory in vitro using the scratch assay. Results: There were significant differences in migratory speed rate between normal and inflamed hDPSCs at 6 and 24 hours (p<0.05). There were significant differences in wound width in each group of normal and inflamed hDPSCs at 6 and 24 hours (p<0.05). Conclusion: These research results show that pulp remains have the natural potential to induce migration in conditions inflamed by LPS bacteria E. coli for 24 hours."

"Latar Belakang: Ekstrak jintan putih Cuminum cyminum memiliki potensi efektivitas antibakteri dan anti jamur serta tidak toksik terhadap sel fibroblas tikus. Belum terdapat penelitian yang meneliti toksisitas ekstrak jintan putih terhadap Dental Pulp Stem Cells DPSCs . Tujuan: Mengetahui efek ekstrak jintan putih konsentrasi 0,1 mg/ml, 0,4 mg/ml, 0,7 mg/ml, dan 1,0 mg/ml terhadap viabilitas DPSCs. Metode: Menggunakan uji MTT dengan menghitung nilai absorbansi menggunakan microplate reader, dengan hasil akhir berupa nilai optical density OD yang dipersentasekan terhadap kelompok kontrol. Hasil: Terdapat perbedaan viabilitas DPSCs yang bermakna

---

Introduction The extract of cumin Cuminum cyminum has the potential antibacterial and antifungal activity and it was not toxic for mouse fibroblasts. However, there have been no research investigating the toxicity of cumin extract on Dental Pulp stem Cells DPSCs . Aims To compare viability DPSCs of Cuminum cyminum extract 0,1 mg ml, 0,4 mg ml, 0,7 mg ml, and 1.0 mg ml . Methods Cell viability was analyzed using MTT Assay by calculating absorbance value using microplate reader, with optical density OD as the final result. Results There were significant differences statistically in viability on DPSCs p"

"Objective: To compare the importance of storage time and the tooth type for isolation of dental pulp cells (DPCs) from extracted human teeth.Methods: 35 human teeth were used in this study. The teeth were stored in phosphate buffered saline (PBS) after extraction and divided into two groups randomly according to the time elapsed between extraction and isolation. In group one, the isolation was performed within 2 hours and in the other group it was performed 24 hours after extraction.Results: No significant differences between isolation time and total cell counts (p 0.483) and between isolation time and viable cells (p 0.341). No significant differences between the first molar and the premolar related cell counts and viable cells, but both teeth groups showed significant higher viability and had higher total cell amounts than third molars after isolation. Statistically significant correlations were found between age of donors and viable cells and viability after 24 hours isolation time.Conclusion: The immediate isolation of DPCs is not necessary after the tooth extraction. The tooth can be stored in PBS at room temperature up to twenty four hours after the extraction without a significant reduction in cell viability and counts. The cells obtained from younger donors might have more chance for more viability even if storage time was extended. Premolars and first molars were better donors than the third molars for DPCs isolations and the high number of success revascularization rate in premolars with necrotic immature premolars might be because of their high cell viability potentials."

"Triethylene glycol dimethacrylate (TEGDMA) is a common component of the bonding agents and resin composites used in dentistry for restorative dentistry. However, TEGDMA could be released from composite resins following incomplete polymerization and degradation processes by salivary enzymes in the mouth. Subsequently, TEGDMA is available in saliva and diffuses toward and affects the dental pulp which contains various cells, and thus may cause severe cytotoxic effects. Objectives: To determine the total protein concentration of human dental pulp cells following exposure to TEGDMA. Materials & methods: Dental pulp cells were isolated from the pulp of the freshly extracted teeth and cultured in DMEM for 48 h (37°C, 5% CO2). Then, 2 mM, 4mM and 8 mM TEGDMA were added to these cells and incubated for 24 h. The total protein was measured by Bradford Protein Assay. Results: The total protein concentration of dental pulp cell after expsured to 4 mM, 8mM, and 12 mM TEGDMA were statistically lower (22762.27 ug/ml ± 3385.87; 20268.44 ug/ml ± 1701.14; 23706.51 ug/ml ± 3214.52; respectively) than the control group (24253.77 ug/ml ± 3072.88). Furthermore, the total protein concentration of culture medium after exposured to 4 mM, 8mM, and 2 mM TEGDMA, were statustically higher (28635 ug/ml ± 2373.4; 35288.41 ug.ml ± 3469.48; 38199.79 ug/ml ± 2752.47; respectively) when compared with the controls (27073.85 ug/ml ± 2772.47). Conclusion: 2 mM, 4 mM, and 8 mM TEGDMA caused cytotoxicity to human dental pulp cells showed by decreasing the total protein of cells and increasing total protein of the culture medium."