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Amal Hayati
Insidensi occult metastasis kelenjar getah bening pada karsinoma ovarium dan hubungannya dengan tipe histopatologik = Incidence of lymph nodes occult metastases in ovarian carcinoma and its association with histopathologic type.
"Latar Belakang: Karsinoma ovarium merupakan tumor ganas ginekologik yang paling mematikan. Metastasis kelenjar getah bening ditemukan pada 78% kasus stadium lanjut yang dilakukan sampling atau diseksi kelenjar getah bening. Metastasis ini juga ditemukan pada kasus yang secara klinis sesuai dengan stadium I dan II. Faktor risiko terjadinya metastasis kelenjar getah bening pada karsinoma ovarium meliputi tipe histologik serosum, tumor high grade, dan kadar CA125 serum yang tinggi pada saat diagnosis. Pemeriksaan ulang kelenjar getah bening negatif pada kasus keganasan ginekologik lain menunjukkan adanya mikrometastasis hingga 8%- 14%.
Tujuan: Penelitian ini dilakukan untuk mengetahui insidensi occult metastasis, baik berupa mikrometastasis maupun isolated tumor cells, pada kelenjar getah bening negatif dengan menggunakan pulasan imunohistokimia sitokeratin AE1/AE3 pada karsinoma ovarium dengan berbagai tipe histologik.
Metode: Penelitian retrospektif dengan desain potong lintang pada sediaan kelenjar getah bening negatif dari operasi histerosalpingoovorektomi disertai limfadenektomi kasus karsinoma ovarium di RSCM periode Januari 2016 sampai Desember 2020. Pada seluruh blok parafin berisi kelenjar getah bening negatif berukuran >1 cm dilakukan potong dalam dua kali untuk masing-masing dipulas hematoksilin-eosin dan imunohistokimia sitokeratin AE1/AE3. Data imunoekspresi AE1/AE3 dianalisis untuk menentukan mikrometastasis/isolated tumor cells, dan mengetahui hubungannya dengan tipe histologik serosum.
Hasil: Dari 57 sampel yang memenuhi kriteria inklusi dan eksklusi didapatkan rerata usia 49,5 tahun. Tipe histopatologik terbanyak (40,3%) adalah karsinoma sel jernih, 66,7% kasus memiliki tumor high grade, dan 57,9% kasus terdiagnosis pada stadium dini. Occult metastasis didapatkan pada 1 (1,75%) kasus dari seluruh sampel. Tidak ditemukan perbedaan kejadian occult metastasis pada kelompok karsinoma serosum dan non-serosum (p=1).
Kesimpulan: Insidensi occult metastasis kelenjar getah bening sebesar 1,75% dari seluruh kasus karsinoma ovarium dalam penelitian ini. Tidak ditemukan perbedaan kejadian occult metastasis pada kelompok karsinoma serosum dan non-serosum.
Background: Ovarian carcinoma is the most lethal gynecologic malignant tumor. Lymph node metastases were found in 78% of advanced stage cases that underwent lymph node dissection. These metastases were also found in cases with clinical stage I and II. Risk factors for lymph node metastasis in ovarian carcinoma include serous histologic type, high grade tumor, and high serum CA125 level at diagnosis. Reexamination of negative lymph nodes in cases of other gynecologic malignancies shows micrometastases in up to 8%-14%.
Aim: This study was conducted to determine the incidence of occult metastases, either in the form of micrometastases or isolated tumor cells, in negative lymph nodes by using cytokeratin AE1/AE3 immunohistochemistry in ovarian carcinomas of various histologic types.
Method: A retrospective study with a cross-sectional design on negative lymph node preparations from hysterosalpingoovorectomy surgery accompanied by lymphadenectomy for ovarian carcinoma cases at RSCM January 2016-December 2020. All paraffin blocks containing negative lymph nodes measuring >1 cm were cut in two sections and stained with hematoxylin-eosin and cytokeratin AE1/AE3. AE1/AE3 immunoexpression data were analyzed to determine micrometastases/isolated tumor cells, and their relationship with serous histological type.
Result: Of the 57 samples that met the inclusion and exclusion criteria, the mean age was 49.5 years. The most histopathological types (40.3%) were clear cell carcinomas, 66.7% cases had high grade tumors, and 57.9% cases were diagnosed at an early stage. Occult metastases were found in 1 (1.75%) cases of the entire sample. There was no difference in the incidence of occult metastases in the serous and non-serous carcinoma groups (p=1).
Conclusion: The incidence of occult lymph node metastases was 1.75% of all ovarian carcinoma cases in this study. There was no difference in the incidence of occult metastases in the serous and non-serous carcinoma groups.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
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UI - Tugas Akhir  Universitas Indonesia Library
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Stella Marleen
Hubungan antara ekspresi CD44 dengan derajat keganasan karsinoma mukoepidermoid kelenjar liur = Association of CD44 expression in salivary gland mucoepidermoid carcinoma with histological grading
"

Latar belakang: Karsinoma mukoepidermoid merupakan keganasan pada kelenjar liur yang paling sering ditemukan. Prognosis karsinoma mukoepidermoid berhubungan dengan derajat keganasannya. Cancer stem cell (CSC) diduga berperan dalam patogenesis karsinoma mukoepidermoid sehingga terjadi resisten terhadap berbagai terapi. CD44 merupakan salah satu penanda SC yang paling banyak pada kelenjar liur dan tampak meningkat pada karsinoma mukoepidermoid. Namun, peran prognostik CD44 pada keganasan masih menjadi perdebatan.

Metode: Penelitian menggunakan metode potong lintang. Sampel terdiri atas 34 kasus di Departemen Patologi Anatomik FKUI/RSCM tahun 2012 sampai 2017. Dilakukan pulasan CD44 dan perhitungan H-score dan presentasi setiap kasus. Hasil perhitungan dikelompokan menjadi ekspresi negatif/positif lemah dan positif kuat.

Hasil: Hasil penelitian menunjukkan bahwa ekspresi CD44 berhubungan secara signifikan dengan derajat keganasan (p=0,006). Ekspresi positif kuat ditemukan lebih banyak pada derajat keganasan rendah dan ekspresi negatif/positif lemah ditemukan lebih banyak pada derajat keganasan tinggi.

Kesimpulan: Ekspresi CD44 pada karsinoma mukoepidermoid berhubungan dengan derajat keganasan.

 

 

Background: Mucoepidermoid carcinoma is the most common malignancy in salivary gland. The prognosis correlates with its histological grading. Cancer stem cell (CSC) is predicted to have a role in pathogenesis of mucoepidermoid carcinoma, thus it make resistent to various therapy. CD44 is one of stem cell (SC) marker that expressed in salivary gland and seemed to be increased in mucopidermoid carcinoma. However, prognostic role of CD44 in malignancy still controversy.

Method: This is a cross sectionsl study. Samples consist of 34 cases from Anatomical Pathology Department, Faculty of Medicine Universitas Indonesia, Ciptomangunkusumo General Hospital in 2012 until 2017. CD44 staining was done and calculated wih H-score method. Then, the samples is catagorized into negative/weak expression and strong expresion.

Result: The result showed that CD44 expression associate significantly with histological grading (p=0,006). Strong expression is found more in low grade and negative/weak expresion is found more in high grade.

Conclusion: CD44 expression in mucoepidermoid carcinoma associates with histological grade.

Keyword: mucoepidermoid carcinoma, histological grade, cancer stem cell, CD44.

 

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Depok: Fakultas Kedokteran Universitas Indonesia, 2018
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UI - Tesis Membership  Universitas Indonesia Library
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Fauziah Fardizza
Penetapan potensi metastasis kelenjar getah bening lokoregional pada karsinoma sel skuamosa laring: pemeriksaan ekspresi biomarka HPV, EGFR, MMP-9, timp-1, vegf, e-kaderin, dan kolagen tipe IV = Potential metastatic locoregional lymph nodes on squamous cell carcinoma larynx examination of biomarker expression HPV, EGFR, MMP 9 timp 1 vegf e cadherin and collagen type IV / Fauziah Fardizza
"ABSTRAK
Biomarka untuk memprediksi metastasis KGB lokoregional sampai saat ini belum akurat.
Angka metastasis tersamar pada karsinoma laring bervariasi yaitu 165%. Dibutuhkan
biomarka tumor yang dapat memberikan informasi adanya metastasis KGB lokoregional
pada pasien KSS laring stadium lanjut tanpa keterlibatan KGB lokoregional (N0), sehingga
diharapkan menjadi acuan untuk dilakukan diseksi leher selektif. Beberapa biomarka yang
berhubungan dengan agresivitas dan prediksi metastasis yaitu Epidermal Growth Factor
Receptor (EGFR), Matrix Metallo-proteinase (MMP)-9, Tissue Inhibitor Metallproteinase
(TIMP)-1, Vascular Endothelial Growth Factor (VEGF), Epithel Calcium Adhesi (Ekaderin)
dan kolagen tipe IV serta HPV dapat digunakan untuk memprediksi luaran pada
status pasien tumor dengan dan tanpa metastasis.
Penelitian ini ingin memeriksa peran infeksi HPV sebagai faktor onkogenesis dan
kejadian metastasis KGB leher pada keganasan laring berdasarkan biomarka sebagai
penetapan diagnosis metastasis KGB lokoregional.
Dilakukan Cross-sectional, double blind study dengan pengumpulan data sekunder dari
rekam medis di Departemen THT-KL FKUI-RSCM. Pemeriksaan ekspresi biomarka
dan status HPV dilakukan terhadap jaringan berupa blok parafin dari pasien karsinoma
laring Ekspresi biomarka dilakukan dengan pemeriksaan imunohistokimia, dan identifikasi
virus HPV dengan nested PCR, dilanjutkan dengan flow-through hybridization.
Didapatkan proporsi HPV KSS laring sebanyak 28,7% dengan infeksi HPV risiko tinggi
sebanyak 9,15% dan HPV 16 merupakan tipe yang terbanyak. Analisis multivariat
Mantel-Haenszel didapatkan ekspresi tinggi biomarka EGFR, MMP-9 dan VEGF
berperan terhadap kejadian metastasis KGB pada KSS laring stadium lanjut tanpa
infeksi HPV dengan OR 3,38; 5,14. Keadaan tersebut tidak berperan lagi bila terdapat
infeksi HPV Dari penelitian ini didapatkan suatu algoritma penatalaksanaan KSS laring
stadium lanjut khususnya untuk penentuan tatalaksana diseksi leher pada N0.
Infeksi HPV didapati pada KSS laring stadium lanjut, HPV 16 merupakan tipe HPV
yang terbanyak. Biomarka penanda metastasis didapatkan pada EGFR; MMP-9; VEGF
dengan kekuatan 2;1;6.
ABSTRACT
Biomarkers to predict locoregional lymph nodes metastasis is not yet accurate until
now. The number of occult metastasis in laryngeal carcinoma varies between 165%.
A tumor biomarker that can give information on the existence of locoregional lymph
node involvement in patients with or without signs of clinical locoregional lymph node
involvement, as guidelines whether selective neck dissection is needed in N0 cases. For
patients that need additional treatment biomarkers that are correlated with aggresivity
and metastasis prediction such as EGFR, MMP-9, TIMP-1, VGEF, E-cadherin, collagen
Type IV and HPV are also needed to predict the outcome of patients with or without
lymph node metastasis.
This study aimed to investigate the evidence of HPV infection in laryngeal carcinoma
and the role of biomarkers EGFR, MMP-9, TIMP-1, VEGF, E-cadherin and collagen
type IV, in a late stadium laryngeal SCC observed clinically, especially in N0 and also
to predict diagnosis of a locoregional lymph node that has potential for metastasis.
Cross-sectional, double blind study with planned data collection was performed in the
Department of ENT FKUI-RSCM. Data were taken from Formalin Fixed Paraffin
Embedded (FFPE) of laryngeal cancer specimen after laryngectomies. Samples were
analysed by nested Polymerase Chain Reaction (PCR) and continuous flow-through
hybridizationed for genotyping. Expression of EGFR, MMP-9, TIMP-1, VEGF, Ecadherin,
and collagen Type IV as metastasis biomarker were evaluated by
immunohistochemistry.
Overall HPV proportion in laryngeal cancer was 28.7%. A total of 9,15% laryngeal
cancer patients were infected with high risk HPV type and HPV 16 was the most
frequently observed. Mantel-Haenszel multivariate analysis found that HPV infection did
not play role in neck metastasis eventhough there were positive evidence of metastasis
biomarker. In contrast, the absent of HPV infection, positif metastasis biomarker of EGFR
and VEGF have risk for neck nodes metastasis with OR 3.38; 5.14 fold consecutively.
The algorithm was formed from the PM model to determine the metastasis potential to
locoregional lymph nodes of late stadium laryngeal SCC with N0.
HPV was found to be the oncogenic factor of the laryngeal SCC and HPV 16 was the
most frequently observed type in laryngeal SCC. Biomarkers to predict locoregional
lymph nodes metastasis are EGFR; VEGF with strenght 2;1;6."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2016
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UI - Disertasi Membership  Universitas Indonesia Library
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Ditha Diana
Peningkatan ekspresi siklin d1 pada karsinoma papiler tiroid dengan metastasis kelenjar getah bening leher dan metastasis tulang = The Increase of cyclin d1 expression in papillary thyroid carcinoma with neck lymph node metastasis and bone metastasis
"ABSTRAK
Latar belakang: Karsinoma papiler tiroid KPT merupakan 85-90 dari seluruhkanker tiroid. KPT adalah tumor epitel folikel ganas yang menunjukkan diferensiasisel folikular disertai perubahan inti dan/ atau pembentukan struktur papiler. Siklin D1dapat digunakan sebagai penanda invasi sel tumor. Tujuan penelitian inimembandingkan ekspresi siklin D1 pada kelompok KPT tanpa metastasis, KPTdengan metastasis ke kelenjar getah bening KGB leher, dan KPT dengan metastasistulang.
Metode: Penelitian menggunakan metode potong lintang. Sampel terdiri atas 13kasus untuk masing-masing kelompok KPT tanpa metastasis, KPT dengan metastasisKGB leher, dan KPT dengan metastasis tulang. Dilakukan pulasan siklin D1 danpenilaian pulasan berdasarkan intensitas dan jumlah inti sel yang terpulas. Dilakukanpenghitungan histoscore dan persentase setiap kasus. Hasil penghitungandikelompokkan menjadi derajat satu yaitu hasil kurang dari 10 , derajat dua yaituhasil 10-50 , dan derajat tiga yaitu lebih dari 50.
Hasil: Hasil penelitian menunjukkan bahwa hasil pulasan siklin D1 derajat tigaditemukan pada sebagian besar kelompok KPT dengan metastasis tulang dan tidakditemukan pada KPT tanpa metastasis. Terdapat hubungan yang bermakna antaraekspresi siklin D1 pada kelompok KPT tanpa metastasis, kelompok KPT denganmetastasis KGB leher, dan KPT dengan metastasis tulang interval kepercayaan 95 0,01-0,74; p
ABSTRACT
Background: Papillary thyroid carcinoma PTC represents 85 of all thyroidcancer. PTC is a malignant epithelial tumor showing evidence of folliculardifferentiation and characterized by distinctive nuclear features with or withoutpapillary structures. Cyclin D1 can be used as a marker of tumor cell invasion. Theaim of the study was to compare expression of cyclin D1 in PTC without metastasisgroup, PTC with neck lymph node metastasis, and PTC with distant metastasis.
Methods: This was cross sectional study. Samples consist of 13 cases for each groupPTC without metastasis, PTC with neck lymph node metastasis, and PTC with distantmetastasis stained with cyclin D1 antibody. Quantification was based on the intensityand distribution of nuclear staining. The appraisal was done with estimatinghistoscore and percentage. Calculation result was graded as follows grade one isfewer than 10 of tumor cells, grade two is 10 50 , and grade three is more than50.
Results: The result of the study shows that grade three of cyclin D1 staining found inmajority cases of PTC with distant metastasis and not found in PTC withoutmetastasis. There is a significant differences of cyclin D1 expression among group ofPTC without metastasis, PTC with neck lymph node metastasis, and PTC with distantmetastasis 95 confidence interval 0,01 0,74 p."
2017
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UI - Tugas Akhir  Universitas Indonesia Library
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Erna Kristiani
Imunoekspresi braf V600E pada karsinoma papiler tiroid dan hubungannya dengan faktor-faktor prognostik serta varian histopatologik = Braf V600E immunoexpression in thyroid papillary carcinoma and its association with prognostic factors and histopathologic variants / Erna Kristiani
"[ABSTRAK
Latar Belakang: Karsinoma papiler tiroid (KPT) merupakan keganasan tersering
organ endokrin dengan prognosis yang sangat baik, namun pada beberapa kasus
dapat terjadi rekurensi dan mortalitas. Beberapa faktor prognostik dan mutasi Btype
rapidly accelerated fibrosarcoma V600E (BRAF V600E) dikatakan
berhubungan dengan prognosis yang lebih buruk. Pemeriksaan imunohistokimia
protein BRAF V600E dipercaya dapat mendeteksi adanya mutasi dengan
spesifisitas 100% dan sensitivitas 89%. Tujuan penelitian ini untuk mengetahui
imunoekspresi BRAF V600E dan hubungannya dengan faktor-faktor prognostik.
Bahan dan Cara: Penelitian dilakukan secara retrospektif, desain deskriptif
analitik studi potong lintang. Sampel penelitian berasal dari RSCM berjumlah 50
kasus KPT yang dinilai ulang untuk menentukan faktor-faktor prognostik secara
mikroskopik. Pemeriksaaan mutasi BRAF V600E menggunakan tehnik
imunohistokimia dan penilaian menggunakan H score.
Hasil: Nilai H score ³ 326,5 ditentukan sebagai mutasi BRAF V600E positif dan
< 326,5 sebagai mutasi BRAF V600E negatif. Terdapat 17 (34%) kasus positif
mengalami mutasi BRAF V600E. Rerata usia pada kasus dengan mutasi BRAF V600E
positif adalah 44,71 tahun. Ukuran tumor pada kasus dengan mutasi BRAF V600E positif
berkisar antara 0,1-4cm. Tujuh belas kasus yang mengalami mutasi, 6 dlaki-laki dan 11
perempuan. Tujuh kasus dengan perluasan keluar tiroid, 11 kasus dengan metastasis
kelenjar getah bening (KGB), dan 8 kasus dengan varian histopatologik tall cell.
Kesimpulan: Terdapat hubungan bermakna antara mutasi BRAF V600E dengan
perluasan keluar tiroid, metastasis kelenjar getah bening (KGB), dan varian
histopatologik tall cell. Tidak terdapat hubungan bermakna antara mutasi BRAF
V600E dengan usia, jenis kelamin, dan ukuran tumor.
ABSTRACT
Backgroud: Papillary Thyroid Carcinoma (PTC) is the most common malignant
neoplasm of the endocrine organ with an excellent prognosis, but in some cases
present with recurrency and mortality. There are prognostic factors and BRAF
V600E mutation that related to worse prognosis. Immunohistochemical
investigetion of BRAF V600E protein believe can detect mutation wth 100%
specificity and 89% sensitivity. Recent study suggest PTC with BRAF V600E
mutation do thyroidectomy with prophylactic lymph node dissection. BRAF
V600E specific inhibitor effective to the patient with advance stadium, patient
with metastases, and resistant to iodium radioactive. Aim of this study is to obtain
BRAF V600E and the relation with prognostic factors.
Material and Methods: This is a retrospective descriptive-analytic crosssectional
study. Fifty patient with PTC comes from Ciptomangunkusumo Hospital
and reviewed to determine prognostic factors microscopicaly. BRAF V600E
mutation detected by immunohistochemical staining and assesed with H score.
Result: H score ³ 326,5 determined as positive BRAF V600E mutation and <
326,5 as negative BRAF V600E mutation. BRAF V600E mutation was detected
in 17 (34%) cases by immunohistochemistry. The mean age of the cases with
positive BRAF V600E mutation was 44.71 years, while the negative 41.58. The
size of the tumor in cases with BRAF V600E mutation positive range between
0,1-4cm, while negative 0,1-9cm. Seventeen cases have mutations, 6 of them sex
male and 11 female. Seven cases with extrathyroidal extension (ETE), 11 cases
with lymph node metastasis (KGB), and 8 cases with tall cell variant.
Conclusion: There are significant correlation between BRAF V600E mutation
with extrathyroidal extension, lymph node metastases, and tall cell variant. There
are no significant correlation between BRAF V600E mutation with age, gender,
and size of the tumor, Backgroud: Papillary Thyroid Carcinoma (PTC) is the most common malignant
neoplasm of the endocrine organ with an excellent prognosis, but in some cases
present with recurrency and mortality. There are prognostic factors and BRAF
V600E mutation that related to worse prognosis. Immunohistochemical
investigetion of BRAF V600E protein believe can detect mutation wth 100%
specificity and 89% sensitivity. Recent study suggest PTC with BRAF V600E
mutation do thyroidectomy with prophylactic lymph node dissection. BRAF
V600E specific inhibitor effective to the patient with advance stadium, patient
with metastases, and resistant to iodium radioactive. Aim of this study is to obtain
BRAF V600E and the relation with prognostic factors.
Material and Methods: This is a retrospective descriptive-analytic crosssectional
study. Fifty patient with PTC comes from Ciptomangunkusumo Hospital
and reviewed to determine prognostic factors microscopicaly. BRAF V600E
mutation detected by immunohistochemical staining and assesed with H score.
Result: H score ³ 326,5 determined as positive BRAF V600E mutation and <
326,5 as negative BRAF V600E mutation. BRAF V600E mutation was detected
in 17 (34%) cases by immunohistochemistry. The mean age of the cases with
positive BRAF V600E mutation was 44.71 years, while the negative 41.58. The
size of the tumor in cases with BRAF V600E mutation positive range between
0,1-4cm, while negative 0,1-9cm. Seventeen cases have mutations, 6 of them sex
male and 11 female. Seven cases with extrathyroidal extension (ETE), 11 cases
with lymph node metastasis (KGB), and 8 cases with tall cell variant.
Conclusion: There are significant correlation between BRAF V600E mutation
with extrathyroidal extension, lymph node metastases, and tall cell variant. There
are no significant correlation between BRAF V600E mutation with age, gender,
and size of the tumor]"
2015
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UI - Tesis Membership  Universitas Indonesia Library
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Romi Beginta
Hubungan ekspresi p53 dan Ki67 dengan parameter histopatologik prediktif metastasis pada karsinoma sel skuamosa penis = The association of expression of p53 and Ki67 to metastasis predictive histopathologic parameter in squamous cell carcinoma of penis / Romi Beginta
"ABSTRAK
Latar belakang: Penentuan faktor risiko metastasis kelenjar getah bening dan prognosis pasien Karsinoma Sel Skuamosa (KSS) penis tidak sebanyak KSS pada organ lain. Penggunaan parameter patologik, ekpresi p53 dan Ki67 dapat digunakan sebagai variabel penentu prognosis maupun terapi KSS penis namun masih diperlukan data yang lebih banyak. Penelitian ini bertujuan untuk menjelaskan hubungan antara ekspresi p53 dan Ki67 terhadap parameter histopatologik yang mempengaruhi risiko metastasis.
Bahan dan Cara: Penelitian ini merupakan studi potong lintang dengan melakukan pulasan imunohistokimia p53 (Novocastra DO-7) dan Ki67 (Biocare CRM 325) pada 25 sampel KSS penis.
Hasil: Ekspresi p53 positif ditemukan pada 48% KSS penis dan ekspresi Ki67 tinggi ditemukan pada 52% kasus. Tidak temukan hubungan yang bermakna antara ekspresi p53 dan parameter-parameter histopatologik. Didapatkan hubungan bermakna antara ekspresi Ki67 terhadap derajat diferensiasi tumor dan adanya invasi uretra.
Kesimpulan: Ekspresi p53 tidak dapat digunakan sebagai faktor prediktif risiko metastasis KSS penis. Ekspresi Ki67 secara sebagian berhubungan dengan faktor risiko metastasis KSS penis.
ABSTRACT
Background: Determination of lymph node metastasis risk factors and prognosis of patients with Squamous Cell Carcinoma(SCC) of the penis is not as much as SCC in other organs. Pathological parameters, expression of p53 and Ki67 could be used as a determinants of prognosis and therapy in SCC of the penis but more data is still needed. This study aims to clarify the relationship between the expression of p53 and Ki67 to histopathological parameters that affect the risk of metastasis.
Methode: This study was a cross-sectional study by using immunohistochemical staining of p53 (Novocastra DO-7) and Ki67 (Biocare CRM 325) in 25 samples of SCC of the penis
Result: Expression of p53positive was found in 48% of SCC of the penis and higher expression of Ki67f was found in 52% of cases. No significant association between p53 expression and histopathologic parameters. Obtained significant correlation between the expression of Ki67 on the degree of tumor differentiation and invasion urethra.
Conclusion: P53 expression can not be used as a predictive factor of risk metastatic in SCC of the penis. Ki67 expression is partially associated with risk factors for metastatic SCC of the penis."
Fakultas Kedokteran Universitas Indonesia, 2014
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UI - Tesis Membership  Universitas Indonesia Library
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Elizabeth Indria Sari
Hubungan carcinoma percentage cp dan lymphatic microvessel density lmvd berdasarkan d2-40/podoplanin sebagai faktor prognostik metastasis ke kelenjar getah bening (KGB) pada adenokarsinoma not otherwise specified nos kolorektal = correlation between carcinoma percentage cp and lymphatic microvessel density lmvd based on d2 40 podoplanin as a prognostic factor for lymph node metastasis in adenocarcinoma not otherwise specified nos colorectal
"ABSTRAK
Latar Belakang:Pada KKR, miofibroblas merupakan komponen sel utama dalam stroma desmoplastik yang memiliki peran penting dalam proses metastasis. Persentase antara karsinoma dengan stroma desmoplastik dikenal sebagai Carcinoma Percentage CP berperan sebagai independent predictor metastasis. D2-40/ Podoplanin PDPN dikenal sebagai marker spesifikLymphatic Endothelial Cell LEC , digunakan untuk menilai Lymphatic Microvessel Density LMVD danLymphatic Vessel Invasion LVI . Tujuan penelitian untuk mengetahui korelasi dan hubungan CP, LMVD dan LVI dengan metastasis sel tumor ke kelenjar getah bening KGB .Bahan dan Metode:Dilakukan penilaian CP terhadap 44 sampel adenokarsinoma Not Otherwise Specified NOS kolorektal dan pulasan D2-40/Podoplanin untuk menilai LMVD dan LVI. Uji statistik dilakukan untuk mencari korelasi antara CP dan LMVD, serta hubungan antara CP, LVI serta metastasis KGB.Hasil:Terdapat korelasi kuat antara CP dan LMVD area intratumoral dan peritumoral dengan arah korelasi negatif. Terdapat hubungan bermakna p=0,00 antara LMVD area intratumoral dan area peritumoral dengan adanya LVI. Terdapat hubungan bermakna antara LVI dengan kejadian metastasis KGB p=0,03 . Area intratumoral menunjukkan hubungan bermakna dengan kejadian metastasis KGB nilai p=0,04 , sedangkan area peritumoral tidak menunjukkan hubungan bermakna nilai p=0,17 .Kesimpulan:Pemeriksaan CP pada sediaan histopatologi dapat digunakan untuk memprediksi tinggi/rendahnya kejadian metastasis sel tumor ke KGB, didasarkan adanya korelasi kuat antara CP dan LMVD. Kata kunci:Carcinoma Percentage, Lymphatic Microvessel Density, Lymphatic Vessel Invasion, Lymphatic Endothelial Cell
ABSTRACT
Background In CRC, myofibroblast are the main component cells in tumour stroma which have an important role in the metastases process. The precentage between carcinoma and desmoplastic stroma known as Carcinoma Percentage CP , can be used as an independent predictor metastases. D2 40 Podoplanin PDPN known as a spesific marker forLymphatic Endothelial Cell LEC , which used to assess Lymphatic Microvessel Density LMVD and Lymphatic Vessel Invasion LVI . This study aims to determine correlation and association between CP, LMVD and LVI with the metastases process to lymph node LN .Materials and Methods CP assessment conducted on 44 samples of adenocarcinoma Not Otherwise Specified NOS colorectal and examination D2 40 Podoplanin to assess LMVD and LVI. The statistical test is performed to find the correlation between CP and LMVD, as well as the relationship between CP, LVI and metastasis KGB.Result There were a strong correlation between CP and LMVD intratumoral and peritumoral area with the negative correlation. There were a significant association p 0,00 between LMVD intratumoral and peritumoral area with the LVI. There was a significant association between LVI and lymph node metastases p 0,03 . Intratumoral area showed significant association with lymph node metastases nilai p 0,04 , whereas peritumoral area showed no significant association nilai p 0,17 .Conclusion CP examination in histopathology specimen can be used to predict high low rate of tumour cells metastases to the lymph node, based on a strong correlation between CP and LMVD. "
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2016
T58903
UI - Tesis Membership  Universitas Indonesia Library
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Matheus Jorizal
Pengobatan radiasi pada karsinoma prostat
"ABSTRAK
Pada makalah ini akan dikemukakan pengobatan radiasi pada karsinoma prostat, dengan suatu laporan retrospektif pengeobatan radiasi pada pasien yang dikirim ke Unit Radioterapi RSCM/FKUI selama periode Januari 1982 sampai dengan Desember 1986.
Kesimpulannya adalah: (1). Penderita karsinoma prostat yang datang berobat ke Subbagian Radioterapi RSCM/FKUI pada umumnya sudah berada pada stadium lanjut, (2). Limfografi penting bukan saja untuk diagnostik tetapi juga dalam hal penanganan terapi, (3). Pengobatan radiasi yang diberikan pada karsinoma prostat umumnya merupakan radiasi pasca bedah, (3). Perlu disusun protokol pengobatan karsinoma prostat.
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1989
T-Pdf
UI - Tesis Membership  Universitas Indonesia Library
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Problems in diagnosis approach for carcinoma of pancreatic head
"Incidences of pancreatic cancer worldwide have been known to be increased. It is the fifth leading cause of death in United State of America.Seventy percent accourts in the head of the pancreas...."
Artikel Jurnal  Universitas Indonesia Library
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Fanny Kamarudy Lay
Imunoekspresi Programmed Cell Death Ligand 1 (PD-L1) pada Karsinoma Tiroid dan hubungannya dengan Tipe Histologik agresif = Programmed Cell Death Ligand 1 (PD-L1) expression in Thyroid Carcinoma and the association with aggressive Histological Type
"Latar Belakang: Karsinoma tiroid merupakan neoplasma organ endokrin yang paling sering terjadi dan sebagian di antaranya memiliki tipe histologik agresif yang masih sulit ditangani hingga kini. Karsinoma pada kelompok ini cenderung menunjukkan resistensi dengan radioablasi I-131 dan terapi dengan pembedahan juga tidak dapat memberikan hasil yang maksimal. Keberadaan imunoterapi dengan menggunakan inhibitor PD-L1 dapat menjadi peluang terapi baru untuk pasien dengan karsinoma tersebut. Namun, penelitian-penelitian tentang PD-L1 pada karsinoma tiroid hingga saat ini masih menunjukkan hasil yang bervariasi dan belum jelas diketahui apakah karsinoma tiroid tipe histologik agresif memiliki imunoekspresi PD-L1 yang lebih tinggi dibandingkan dengan tipe histologik kurang agresif.
Tujuan: Penelitian ini bertujuan untuk mengetahui imunoekspresi PD-L1 pada karsinoma tiroid dan hubungannya dengan tipe histologik agresif dibandingkan dengan tipe histologik kurang agresif.
Metode: Penelitian ini menggunakan desain retrospektif dan potong lintang. Populasi terjangkau penelitian adalah kasus karsinoma tiroid di Departemen Patologi Anatomik FKUI/RSCM periode Januari 2015 hingga Desember 2019. Sampel penelitian dibagi menjadi 2 kelompok, yaitu kelompok karsinoma tiroid dengan tipe histopatologik agresif dan kelompok karsinoma tiroid tipe histologik kurang agresif. Pemeriksaan imunohistokimia dilakukan pada blok parafin kedua kelompok sampel dengan menggunakan antibodi primer monoclonal mouse anti-PD-L1, clone 22C3 (Dako) untuk menilai imunoekspresi PD-L1 yang dinyatakan dalam tumor proportion score (TPS). Nilai TPS dihitung berdasarkan persentase jumlah sel tumor yang terwarnai secara total atau parsial pada membran sitoplasma sel tumor dibagi dengan jumlah seluruh sel tumor dalam satu slaid. Data imunoekspresi PD-L1 kemudian dianalisis untuk mengetahui perbedaan nilai TPS di antara kedua tipe histologik karsinoma tiroid tersebut.
Hasil: Terdapat total 52 kasus karsinoma tiroid yang terdiri atas 26 kasus tipe histologik agresif dan 26 kasus tipe histologik kurang agresif pada penelitan ini. Imunoekpsresi PD-L1 yang dinilai dalam ukuran TPS (tumor proportion score) ditemukan dengan nilai median 0,60% (0%-95,00%) pada kelompok tipe hitologik agresif, dan 0.07% (0%- 19,53%) pada kelompok tipe histologik kurang agresif (P:0,01; IK:95%). Pada analisis tambahan ditemukan juga perbedaan nilai TPS yang signifikan pada variabel perluasan tumor keluar tiroid (P:0,02; IK:95%).
Kesimpulan: Terdapat perbedaan imunoekspresi PD-L1 yang signifikan antara karsinoma tiroid tipe histologik agresif dan kurang agresif. Nilai TPS ditemukan lebih tinggi pada karsinoma tiroid tipe histologik agresif dibandingkan dengan tipe histologik kurang agresif. Temuan ini dapat membuka peluang imunoterapi pada pasien karsinoma tiroid dengan tipe histologik agresif di masa depan.
Background: Thyroid carcinoma (TC) is the most common endocrine organ neoplasm. Some of TCs may show aggressive histological types that are still difficult to treat nowadays. Carcinomas in this group tend to show resistance to I-131 radioablation and surgical therapy also does not provide optimal results. The existence of immunotherapy using PD-L1 inhibitors can be a new therapeutic opportunity for patients with these carcinomas. However, studies on PD-L1 in TC still show varying results and it is not clear whether the aggressive histological type of TC has a higher immunoexpression of PD-L1 than the less aggressive histological type.
Objectives: To investigate the significance of PD-L1 expression in aggressive histological type of TC comprae to the less aggressive type.
Material and methods: The population covered by this retrospective cross-sectional study were TC cases at the Department of Anatomic Pathology, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia, period 2015 - 2019. The cases were categorized into two groups, a group of aggressive histological types and a group of the less aggressive histological types of TC. The immunohistochemical examinations were carried out on paraffin blocks of both sample groups using the mouse monoclonal primary antibody anti-PD-L1, clone 22C3 (Dako) to evaluate the tumor proportion score (TPS) value of PD-L1 expression. The TPS value was calculated based on the number of tumor cells that were fully or partially stained in the cytoplasmic membrane of tumor cells divided by the total number of tumor cells in one slide. Data analysis was performed to determine the significance of PD-L1 expression in the aggressive histological types of TC.
Results: A total of 52 cases of TC consisting of 26 cases of aggressive histological types and 26 cases of less aggressive histological types has been studied. PD-L1 expression was evaluated by calculating the TPS in both groups. We found a significance difference of the median TPS value of 0.60% (0 - 95.00%) in the aggressive histological type group, and 0.07% (0 - 19.53%) in the less aggressive histological type group (P: 0.01; CI: 95%). A significant difference in TPS value was also found for the extrathyroidal extension in an additional analysis (P: 0.02; CI:95%). Conclusions: The present study found a significant association between PD-L1 expression and the aggressive histological type of TC. The TPS values were found to be higher in the group of aggressive histological types of TC compared to the less aggressive histological types. A significant association between PD-L1 expression and the presence of extrathyroidal extension of TC has also been suggested. These findings may open opportunities for future immunotherapy in patients with thyroid carcinoma with aggressive histologic types."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
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UI - Tugas Akhir  Universitas Indonesia Library
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