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"Sel induk(Stem Cell) adalah sel pembangun setiap organ dan jaringan tubtih kita. Dia adalah sel yang belum berdiferensiasi, tapi dengan kondisi tepat mampu berkembang inenjadi jaringan khusus dan organ tertentu. Sebdgian besar dari sel-sel jaringan tidak dapat beregenerasi jika rusak berat atau sakit, seperti pada kasus Infark Miokard. Penelitian terbaru mulai berfokus pada sel induk, yang dapat berproliferasi, dan berdiferensiasi menjadi sel otot jantung. Artikel ini bertujuan untuk ineiierangkan transplantasi sel induk pada infark miokard, keniajuan dan kendala yang terdapat dalam btdang kardiotnioplasti selular. (Med J Indones 2005; 15:3-8).

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Stem cells are the foundation cells for every organ and tissue in the body. They are undifferenciated cells that under proper conditions begin to develop into specialized tissues and organs. Most of the body's specialized cells cannot be replaced by natural processes if they are seriously damaged or diseased, such as in Myocardial Infarction. Recent interest has focused on stem cells, which can proliferate, and differentiate into cardiomyocytes.This paper aim to provide overview of stem cell transplantation in myocardial infarction, milestones and setbacks in the study of cellular cardiomyoplasty. (Med J Indones 2005; 15:3-8)."

"The periodontal ligament (PDL) consists of a group of specialized connective tissue fibers embedded in the alveolar bone and cementum that are believed to contain progenitors for mineralized tissue-forming cell lineages. These progenitors may contribute to regenerative cell therapy or tissue engineering methods aimed at recovery of tissue formation and functions lost in periodontal degenerative changes. Some reports using immortal clonal cell lines of cementoblasts, which are cells containing mineralized tissue-forming cell lineages, have shown that their phenotypic alteration and gene expression are associated with mineralization. Immortal, multipotential PDL-derived cell lines may be useful biological tools for evaluating differentiation-inducing agents. In this study, we confirmed the gene expression and mineralization potential of primary and immortal human PDL cells and characterized their immunophenotype. Following incubation with mineralization induction medium containing β-glycerophosphate, ascorbic acid, and dexamethasone, normal human PDL (Pel) cells and an immortal derivative line (Pelt) cells showed higher levels of mineralization compared with cells grown in normal growth medium. Both cell types were positive for putative surface antigens of mesenchymal cells (CD44, CD73, CD90, and CD105). They were also positive for stage-specific embryonic antigen-3, a marker of multipotential stem cells. Furthermore, PDL cells expressed cementum attachment protein and cementum protein 1 when cultured with recombinant human bone morphogenetic protein-2 or -7. The results suggest that normal and immortal human PDL cells contain multipotential mesenchymal stem cells with cementogenic potential."

"This volume will cover a series of reviews on stem cells including adult and embryonic stem cells. Speakers were invited to present these talks during the Stem Cell Symposia in fall of 2010, in Samsun, Turkey. Unique aspect of this volume is that it brings a multidisciplinary aspect of stem cells extracted from a symposium."

"Neural development and stem cells, provides neuroscientists with a handy guide to stem cells in the nervous system, tracing with great clarity the development of stem cells from differentiation to neurons, astrocytes, and oligodendrocytes."

"This book describes the nuclear reprogramming, chronicling how the field has developed over the last 50-60 years."

"The main objective of this book is to provide a comprehensive review on stem cells and their role in tissue regeneration, homeostasis and therapy. In addition, the role of cancer stem cells in cancer initiation, progression and drug resistance are discussed. The cell signaling pathways and microRNA regulating stem cell self-renewal, tissue homeostasis and drug resistance are also mentioned. Overall, these reviews will provide a new understanding of the influence of stem cells in tissue regeneration, disease regulation, therapy and drug resistance in several human diseases."

"Latar Belakang: L-arginin merupakan asam amino semiesensial yang produksinya tidak mencukupi kebutuhan dalam kondisi stres oksidatif akibat inflamasi. L-arginin adalah satu-satunya substrat bagi enzim nitric oxide synthase (NOS) yang memproduksi nitric oxide (NO) yang dapat mengaktivasi focal adhesion kinase (FAK) pathwaydan memicu terjadinya proses migrasi sel. Tujuan: Mengetahui potensi media kultur asam amino L-arginin terhadap laju kecepatan migrasi hDPSCs. Metode: Evaluasi media kultur asam amino L-arginin konsentrasi 300, 400, 500 ¼mol/L, serta DMEM sebagai kontrol terhadap laju kecepatan migrasi hDPSCs menggunakan uji scratch assay menggunakan uji scratch assay yang dihitung dengan rumus laju kecepatan migrasi setelah 24 jam. Analisis statistic menggunakan Paired T-Test dan Oneway ANOVA dengan post hoc LSD. Hasil: Terdapat perbedaan bermakna potensi L-arginin 500 μmol/L dibandingkan konsentrasi 300 dan 400 μmol/L, serta kontrol. Kesimpulan: Media kultur asam amino L-arginin 500 ¼mol/L memiliki potensi laju kecepatan migrasi yang lebih baik dibandingkan konsetrasi 300, 400 ¼mol/L dan kontrol.

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Background: L-arginine is semiessential amino acid which the production is insufficient under oxidative stress due to inflammation. L-arginine is the only substrate of nitric oxide synthase (NOS) enzyme that produces nitric oxide (NO) which activates focal adhesion kinase (FAK) pathway to stimulate cell migration.

Objective: To understand potential of L-arginine amino acid culture media towards speed rate of hDPSCs migration.

Methods: Evaluation of 300, 400, 500 ¼mol/L of L-arginin amino acid culture media and DMEM as control towars speed rate of hDPSCs migration using scratch assay and calculation of migration speed rate after 24 hours. Statistical analysis using Paired T-Test and Oneway ANOVA with post hoc LSD.

Results: Significant result was shown between 500 ¼mol/L of L-arginin amino acid culture media compared with 300 and 400 ¼mol/L concentration and control towards migration speed rate after 24 hours.

Conclusion: 500 ¼mol/L of L-arginin amino acid culture media has a better migration rate compared with lower concentrations and control."

"Latar Belakang: Pada kondisi inflamasi pulpa, terdapat penurunan dari jumlah protein dan asam amino. L-Arginine adalah asam amino semi-esensial karena berperan penting pada kondisi tertentu, gangguan imun berat dan luka bakar, yang membutuhkan asupan tambahan L-Arginine eksternal. Asam amino L-Arginine menjadi satu-satunya substrat sintesis nitric oxide (NO) dan poliamina berasal dari konversi L-Arginine menjadi ornithinen melalui arginase. NO dan poliamina merangsang proliferasi sel dan memiliki efek positif pada perkembangan melalui siklus sel. Tujuan: Mengetahui potensi asam amino L-Arginine terhadap proliferasi hDPSCs. Metode: Evaluasi asam amino L- Arginine konsentrasi 300, 400, 500 μmol/L, serta DMEM sebagai kontrol terhadap proliferasi hDPSCs menggunakan uji cell count setelah 24 jam. Analisis statistic menggunakan Oneway ANOVA dengan post hoc Bonferroni. Hasil: Terdapat perbedaan bermakna potensi L-Arginine 300,400 dan 500 μmol/L dibandingkan kontrol, dan L- Arginine 500 μmol/L memiliki rerata proliferasi hDPSCs paling tinggi sebesar 436.666 sel/ml. Kesimpulan: Asam amino L-Argininee memiliki potensi terhadap proliferasi hDPSCs dan proliferasi tertinggi pada asam amino L-Arginine konsentrasi 500 μmol/L.

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Background: In the inflammatory condition of the pulp, there is a decrease in the amount of protein and amino acids. L-Arginine is a semi-essential amino acid because it plays an important role in certain conditions, severe immune disorders and burns, which require additional intake of external L-Arginine. The amino acid L-Arginine is the sole substrate for the synthesis of nitric oxide (NO) and polyamines derived from the conversion of L- Arginine to ornithine via arginase. NO and polyamines stimulate cell proliferation and have a positive effect on progression through the cell cycle. Objective: To determine the potential of L-Arginine amino acid on the proliferation of hDPSCs. Methods: Evaluation of L-Arginine amino acid with concentrations of 300, 400, 500 μmol/L, and DMEM as a control for hDPSCs proliferation using cell count test after 24 hours. Statistical analysis using Oneway ANOVA with Bonferroni post hoc. Results: There was a significant difference in the potency of L-Arginine 300,400 and 500 μmol/L compared to control, and L-Arginine 500 mol/L had the highest average proliferation of hDPSCs of 436.666 cells/ml. Conclusion: The amino acid L-arginine has the potential to proliferate hDPSCs and the highest concentration at 500 μmol/L."