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"Studi ini bertujuan menilai peranan CT pada cedera saluran aerodigestif bagian atas. Dua puluh enam pasien dengan cedera saluran aerodigestif atas telah dievaluasi. Enam belas pasien mengalami trauma tumpul pada leher sementara 7 pasien mengalami luka (embus. Sebagian besar pasien datang berobat segera setelah mengalami cedera. Gejala yang timbul meliputi gangguan napas (14), nyeri leher (18), suara serak (7), batuk darah (2) dan kesulitan menelan (3). Cedera jaringan lunak lerdapat pada 8 pasien, udem daerah ariepiglotik pada 4 pasien, hematom ariepiglotik pada 1 pasien, udem pita suara (2)dan obliterasi sinus pirifonnis (5). Cedera daerah supraglotik terjadi pada 6 pasien, cedera glotis (8), dan subglotis (4). Cedera trakea terlihat pada 8 pasien yang terdiri dari pemisahan kriko-trakea (I), robekan trakea (5) dan penyempitan trakea (4). Cedera hipofaring-esofagus terjadi pada 2 pasien. CT bermanfaat memastikan lokasi benda asing yang terjadi pada 2 kasus. CTjuga membantu dalam mengatnbil keputusan pemilihan tata laksana pasien dan menghindari eksplorasi terbuka pada pasien dengan lesi mukosa minimal, fraktur stabil dan robekan tertutup. CT juga lerutama bermanfaat untuk kasus-kasus dimana laringoskopi tidak bisa dilakukan. (Med J Indones 2006; 15:81-9).

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This study was aimed to assess the role of CT in upper aerodigestive tract injuries. Twenty six patients presenting with upper aerodigestive tract injury were examined by CT. Nineteen patients had blunt trauma to the neck while seven had penetrating injury. Most of the patients presented soon after injury. Symptoms included respiratory distress (14), neck tenderness (18), hoarseness (7), haemoptysis (2) and odynophagia (3). Soft tissue injuries were seen in 8 patients, aryepiglottic fold edema in 4, aryepiglottic fold haematoma in 1, vocal cord edema in 2 and pyriform sinus obliteration in 5 patients, Supraglottic injuries were seen in six patients, glottic injury in 8 patients and subglottic injuries in 4 patients. Tracheal injuries were seen in 8 patients and included cricotracheal separation (1), tracheal tears (5) and trachea! narrowing (4). Hypopharyngoesophageal injuries were seen in 2 patients. CT was helpful for localization of foreign bodies in 2 cases. CT is useful in deciding management of patients with upper aerodigestive tract injuries obviating the need of open exploration in patients with minimal mucosal injuries, undisplaced fracture and sealed tears. It is particularly helpful in cases when indirect laryngoscopy was not possible. (Med J Indones 2006; 15:81-9)."

"Studi ini bertujuan menilai peranan CT pada cedera saluran aerodigestif bagian atas."

"This is the first time a book about laparoscopy in emergency abdominal surgery has been published. Numerous articles have been published in specific surgical journals, but, until now, there has not been a book that collates all the aspects of this little-known field. The aim of this volume is to achieve a complete and easy presentation of all the implications associated with laparoscopy in emergency abdominal surgery. The book should be a manual that can be easily consulted by digestive, general, and specialized surgeons, especially in an emergency. The authors’ contributions are founded on evidence-based medicine, which give the book scientific credibility, but this is coupled with their experience of daily practice, which adds an important complementary dimension to evidence-based medicine. This is balanced by an emphasis on clarity and accessibility, because the ultimate aim of the book is educational. "

"This book provides a lucid summary of modern multislice CT imaging of the abdomen, with a focus on the essential imaging findings. After a concise technical introduction, the most important abdominal diseases are described and illustrated with high-quality images. Sections are devoted to the liver and biliary system, the pancreas and spleen, the kidneys and urogenital system, and the bowel and peritoneal cavity. Throughout, key differential diagnostic features are highlighted. The editorial team is composed of internationally renowned radiologists from Europe and the United States, and all chapters have been written by recognized experts in the topic under consideration. Multislice CT of the Abdomen will serve as an excellent reference for radiologists participating in further professional training and will prove an ideal source of information for all who wish to deepen their personal knowledge of the subject."

"Ruang Lingkup dan Cara Penelitian: Peneliti di Indonesia sering mengalami kesulitan memperoleh bahan baku seperti petanda protein untuk menentukan 'berat molekul'. Salah satu petanda protein adalah inhibitor tripsin kacang kedelai (ITK). Penelitian ini bertujuan mengisolasi dan memurnikan ITK dari biji kacang kedelai (Glycine max), Isolasi dilakukan dengan cara ekstraksi asam, diikuti 'salting out, dialisis dan pengendapan aseton. Pemurnian dilakukan dengan kromatografi kolom pertukaran ion. Aktivitas ITK fraksi 'kasar' dan 'murni' diperiksa dengan mengamati pengaruh hambatan terhadap aktivitas enzimatik tripsin, dengan substrat azokasein (proteolitik) dan BAPA/BAPNA (amidase). Azokasein yang dipakai sebagai substrat reaksi tripsin disintesis sendiri. Penilaian kemurnian ITK ?kasar? dan 'murni', juga tripsin dilakukan dengan elektroforesis gel 'slab' SDS-poliakrilamid. Sebagai pembanding pada penelitian ini digunakan inhibitor tripsin produk Sigma.Hasil dan Kesimpulan : Dari 100 g biji kacang kedelai kering panen, diperoleh 1,16 g isolat (ITK 'kasar') setara dengan 418,46 mg protein. Pemurnian lewat kolom pertukaran ion menghasilkan 2 fraksi dengan 'recovery' protein total minimal 63,7 %. Azokasein yang disintesis hasilnya berbeda bila jenis alkohol yang digunakan juga berbeda. Pada penelitian ini baik ITK 'kasar` maupun 'murni' memperlihatkan hambatan terhadap reaksi enzimatik tripsin. Hambatan 50 % terjadi pads rasio inhibitor/enzim yang bervariasi; ITK `murni' 1 memperlihatkan angka yang paling tinggi. Elektroforetogram menunjukkan bercak ITK 'murni' II identik dengan SBTI (Sigma).

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Scope and Method of Study: The chemicals required for laboratory investigations are quite often difficult to obtain, e. g. the standard protein markers for molecular weight determination. Among the markers used for that purpose is the soybean trypsin inhibitor. This work was carried out to isolate and purify trypsin inhibitor from soybean (Glycine max) seeds (SBTI). The procedure included an acid extraction, followed by salting-out, dialysis and an acetone precipitation. Purification was carried out by ion-exchange column chromatography. The protein content of the isolate and of the purified substance was determined by spectrophotometer. The activities of the crude and of the purified soybean trypsin inhibitor were tested against trypsin activity. The trypsin used was obtained commercially. Trypsin's proteolytic activity was performed on azo-casein while its amidase activity was tested on BAPA/BAPNA. The azo-casein was synthesized in the laboratory. The purity of the crude and of the purified soybean trypsin inhibitors, and of the trypsin itself was examined on a slab SDS-polyacrylamide gel electrophoresis (SDS-PAGE).

Findings and Conclusions: From 100 g of dried soy-bean seeds, 1.16 of product was isolated (= 418.46 mg protein). Further purification on an ion-exchange column yielded two fractions, with a minimum of 63.7% protein recovered. The azo-casein synthesis revealed two different products depending on the grade of alcohol used in the process. The crude and the purified soybean trypsin inhibitors showed inhibitory effects towards trypsin. The fifty percent inhibition occurred at varied inhibitor/enzyme ratios, the highest was shown by the purified SBTI I. The electrophoretogram showed that the purified SBTI II was identical to the SBTI (Sigma)."

"Latar belakang. Pabrik semen merupakan salah satu industri yang menerapkan kerja gilir bagi karyawannya untuk meningkatkan produktifitas. Kerja gilir ini berdampak pada gangguan irama sirkadian yang menyebabkan gangguan pencernaan. Di pabrik semen ini, gangguan pencernaan ditemukan pada pekerja gilir yang berotasi. Oleh karena itu dilakukan penelitian dengan tujuan mengetahui prevalensi gangguan pencernaan dan faktor-faktor yang berhubungan dengan gangguan pencernaanMetode penelitian. Berupa studi comparative cross sectional (perbandingan potong lintang) melalui perbandingan prevalensi gangguan pencernaan antara kelompok pekerja gilir dengan pekerja non gilir. Jumlah sampel pada kelompok kerja gilir dan kelompok non gilir masing masing 100 orang. Pengambilan sampel dilakukan secara acak sederhana dari populasi yang memenuhi persyaratan kriteria inklusi. Data penelitian diperoleh melalui wawancara dengan kuesioner, pemeriksaan fisik, rekam medis pekerja dan data dari bagian kepegawaian.Hasil penelitian. Didapatkan gangguan pencernaan pada pekerja gilir dengan pola rotasi dan prevalensi gangguan pencernaan sebesar 11% dengan CI 95% 4,9% - 17.1%. Faktor yang berhubungan paling kuat dengan gangguan pencernaan adalah riwayat penyakit seperti ginjal, hepatitis, tukak lambung dan batu empedu dengan p= 0,001 OR=14,635 CI 95% 2,909 - 73,626. Dan faktor yang juga berpengaruh terhadap timbulnya gangguan pencernaan adalah jumlah hari kerja dalam seminggu dengan p = 0,049 OR = 4,098 CI 95% 1,008 - 16,663 , Variabel penelitian seperti usia pekerja, tingkat pendidikan,jumlah jam kerja dalam sehari, masa kerja, stres, pola makan, kebiasaan merokok dan kebiasaan olah raga pada kedua kelompok kerja tidal( ditemukan perbedaan yang bermakna.Kesimpulan: Dari penelitian ini tidak terbukti bahwa kerja gilir yang berotasi mengakibatkan gangguan pencernaan dan secara statistik terbukti bahwa faktor jumlah hari kerja dalam seminggu dan riwayat penyakit bermakna dalam mempengaruhi timbulnya gangguan pencernaan (p< 0,05 ).Oleh karena itu untuk mencegah dan mengurangi gangguan pencernaan, perlu dilakukan antisipasi dan pengertian yang dalam baik dari pihak manajemen, pekerja maupun dokter perusahaan.

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The Influence Shift Work To Digestive Disorder At Male Worker Part Of Production At Cement Factory PT " X" In Citeureup BogorBack ground Cement factory represent one of the industry applying shift work to its employees to increase productivity. This shift work affect at rhythm trouble of circadian causing digestive disorder. In this cement factory, digestive disorder found at shift worker which is rotation. Therefore this study conduct to identify the prevalence of digestive disorder and other factors related to digestive disorder.

Research method Comparative cross sectional (transversal crosscut comparison) passing comparison of digestive disorder prevalence among group shy worker with non shift worker. Amount of sample at shift worker team and non shy worker team of everyone 100 persons. Intake of sample conducted at random modestly from population fulling conditions of inclusion criteria. Research data obtained from interview with questionnaire, physical examination, medical record and employee data

Result of research. Digestive disorder at shift worker with rotation pattern and the prevalence digestive disorder is II % with CI 95% 4,9 - 17,1%. The most influence factor related to digestive disorder is historical of disease with p = 0,001 OR=14, 635 CI 95% 2,909 - 73,626. And factor having an effect to incidence digestive disorder is amount of workday within a week with p = 0,049 OR = 4,098 CI 95% 1,008 - 16,663. Research variable like worker age, education level, the amount of workhour within a day, year of job, sires, pattern eat, habit smoke, habit of disease history and sport at both working team have equivalent so that not be found by difference having a meaning.

Conclusion. This research didn't proven that rotating shift work caused to digestive disorder and statistically significant relation between amount of workday within a week and historical of disease with digestive disorder (p < 0,05).Therefore to prevent and lessen digestive disorder, a coordination need to conducted between management, company doctor and also worker to improve this matter."

"ABSTRAKAnesthesi spinal pada bedah digestif merupakan tindakan pembedahan yang dapatmempengaruhi keadaan hemodinamik.Pemberian Latihan mobilisasi rom dinidiharapkan mampu membantu menstabilkan keadaan hemodinamik.Tujuanpenelitian ini adalah mengetahui perbedaan mobilisasi 2 jam dan 6 jam pascaoperasi digestif dengan anestesi spinal. Desain penelitian ini adalah quasieksperimen dengan pre-post test non equivalen dengan jumlah 60 responden.Penilaian dilakukan dengan pengukuran tanda vital pada ketiga kelompok,pengukuran pertama yaitu 2 jam post operasi sebelum dilakukan intervensi,pengukuran kedua dilakukan setelah 2 DAFTAR GRAFIKjam setelah diberilatihan mobilisasi dan pengukuran ketiga 6 jam post operasi setelah diberi latihan.Hasil uji anova-post-hoc diperoleh perbedaan nilai p < 0.05, pada kelompokintervensi 2, khusus nilai diastolik dan MAP. Rekomendasi penelitian ini adalahbahwa dapat dipertimbangkan pemberian mobilisasi ROM dini baik 2 jam dan 6jam post operasi bisa dilakukan mobilisasi secara bertahap khususnya pada pasiendengan anestesi spinal pada pembedahan digestif ini.

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ABSTRACTSpinal anesthesia on post digestive surgery patient can influence hemodynamics’patient. The early mobilization: ROM is expected to stabilize hemodynamicpatient post-surgery. The purpose of this study is to determine the difference onhemodynamic status patient who have early mobilization 2 hours and 6 hours postsurgerywith spinal anesthesia. Quasi experiment was used with pre-posttestnonequivalent method which is recruited 60 respondents. Hemodynamic statuses weredocumented by measuring vital signs on 3 different groups. The first measurement wasdocumented on 2 hours post-surgery before intervention. The second measurements wereconducted 2 hours after early mobilization was given and third measurements wereconducted 6 hours post-surgery after mobilization. The result from ANOVA test showssignificant different (p<0.05) between second group who received ROM 2 hours postsurgery,especially on diastolic and MAP score. It is recommended that earlymobilization 2 and 6 hours post-surgery should be given gradually, especially patient withdigestive surgery who received spinal anesthesia"

"Penelitian penapisan beberapa Lactobacillus plantarum dan optimasi produksi protease serupa tripsin (PST) dilanjutkan dengan pemekatan dan karakterisasi parsial telah dilakukan. Tripsin memiliki peran penting dalam pencernaan protein di usus kecil namun produksi tripsin komersial saat ini masih terbatas oleh masalah sertifikasi halal dan risiko penularan penyakit yang bersumber dari babi atau sapi. Penelitian bertujuan menyeleksi koleksi isolat L. plantarum yang menghasilkan aktivitas PST tertinggi dan menentukan kondisi optimum dalam produksi PST dari L. plantarum terpilih menggunakan Response Surface Methodology (RSM) diikuti dengan pemurnian dan karakterisasi parsialnya. Tujuh isolat L. plantarum yang diperoleh dari makanan tradisional Indonesia diseleksi secara kualitatif dan kuantitatif. Semua isolat L. plantarum menunjukkan aktivitas proteolitik, terlihat adanya zona bening di sekitar koloni. Zona bening menunjukkan adanya potensi L. plantarum sebagai sumber PST secara kualitatif. Hasil pengujian kuantitatif menunjukkan bahwa isolat dengan kode B6 (L. plantarum WBM-4) menghasilkan PST dengan aktivitas tripsin tertinggi sebesar 0,16 mU/mL. Lactobacillus plantarum WBM-4 diisolasi dari buah Menteng Banjarmasin paling berpotensi untuk menghasilkan PST. Selanjutnya, L. plantarum WBM-4 dioptimalisasi produksi menggunakan Respon Surface Methodology (RSM) dan karakterisasi PST. Kondisi optimal ditentukan pada komposisi medium dengan 1,96% glukosa, 0,39% yeast extract, 1,97% skim milk, dan pH 6,62, menghasilkan aktivitas PST sebesar 0,303 mU/mL. Pemekatan enzim kasar di bawah kondisi optimum menggunakan viva spin 5000 MWCO meningkatkan kemurnian hingga 11,08 kali lipat, dengan aktivitas sebesar 2,47 mU/mL. Karakterisasi parsial menunjukkan berat molekul PST sekitar ~19 kDa dan ~29 kDa, stabilitas dalam rentang suhu 30 - 40°C, dan aktivitas optimal pada pH 7,0 - 8,0. Penambahan ion logam EDTA, Ca2+, dan Zn2+ memengaruhi aktivitas PST. Penyimpanan PST selama 30 hari pada suhu 4°C aktivitas tersisa PST masih 65% sedang pada suhu 24-28°C aktivitas hanya tersisa 15%. Hasil penelitian ini memberikan gambaran tentang potensi PST yang berasal dari L. plantarum untuk aplikasi suplemen pencernaan dan memberikan alternatif sumber tripsin yang halal dan aman.

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Research on screening of several Lactobacillus plantarum and optimization of trypsin-like protease production (TLP) followed by concentration and partial characterization has been carried out. Trypsin has an important role in protein digestion in the small intestine, but commercial trypsin production is currently limited by halal certification issues and the risk of transmission of diseases sourced from pigs or cattle. The study aimed to select a collection of Lactobacillus plantarum isolates that produced the highest TLP activity and determine the optimum conditions in TLP production from selected L. plantarum using Response Surface Methodology (RSM) followed by purification and partial characterization. Seven isolates of L. plantarum obtained from traditional Indonesian food were selected qualitatively and quantitatively. All L. plantarum isolates exhibited proteolytic activity, with clear zones around the colony. The clear zone shows the potential of L. plantarum as a qualitative source of TLP. The results of quantitative testing showed that isolates with code B6 (L. plantarum WBM-4) produced TLP with the highest trypsin activity value 0.16 mU/mL. L. plantarum WBM-4 isolated from Banjarmasin Menteng fruit has the most potential to produce TLP. Furthermore, L. plantarum WBM-4 optimized production using Response Surface Methodology (RSM) and TLP characterization. Optimal conditions were determined in the composition of the medium with 1.96% glucose, 0.39% yeast extract, 1.97% skim milk, and pH 6.62, resulting in TLP activity of 0.303 mU/mL. Crude enzyme concentration under optimum conditions using viva spin 5000 MWCO increases purity up to 11.08-fold, with an activity of 2.47 mU/mL. Partial characterization shows TLP molecular weights of approximately ~29 kDa and ~19 kDa, stability in the temperature range of 30 - 40 °C, and optimal activity at pH 7.0 - 8.0. The addition of EDTA, Ca2+, and Zn2+ metal ions affect TLP activity. TLP storage for 30 days at 4°C the remaining activity of PST is still 65% while at 24-28°C the activity is only 15%. The results of this study provide an overview of the potential of PST derived from L. plantarum for digestive supplement applications and provide an alternative source of trypsin that is halal and safe."

"Latar Belakang: Secara global diperkirakan terdapat 313 juta pembedahan yang dilakukan, dengan angka kematian 30 hari pascaoperasi mencapai 4.2 juta jiwa. Penilaian kondisi pasien preoperatif diperlukan untuk memprediksi morbiditas danmortalitas pasien pascabedah, maka modalitas yang digunakan dalam menilai risiko pembedahan sebaiknya memiliki akurasi dan objektivitas yang baik. Salah satu modalitas yang rutin digunakan di RSUPN Cipto Mangunkusumo (RSUPNCM) adalah skor ASA-PS. Namun skor ini sudah banyak ditinggalkan oleh negara maju dan beralih pada skor P-POSSUM yang dinilai lebih objektif, dan akurat. Studi ini menguji kesahihan skor P-POSSUM dalam memprediksi lama perawatan pasien pascabedah digestif mayor di ICU, yang mencerminkan keparahan morbiditaspascabedah. Tujuan: Studi ini menguji kemampuan kalibrasi dan diskriminasi skor P-POSSUM dalam memprediksi lama perawatan di ICU, dan menganalisis hubungan antar variabel skor P-POSSUM dengan lama perawatan di ICU pada pasien pasabedah digestif mayor. Metode: Studi ini merupakan studi kohort retrospektif di RSUPNCM selama Januari 2017 hingga Desember 2018. Sebanyak 289 subjek yang sesuai kriteria inklusi dianalisis dari data rekam medis. Lama perawatan pascabedah di ICU dan skor P-POSSUM subjek dicatat sesuai dengan data rekam medis. Variabel PPOSSUM yang berpengaruh terhadap lama perawatan subjek dianalisis dengan analisis bivariat dan regresi logistik multivariat. Kesahihan skor dinilai menggunakan uji kalibrasi Hosmer-Lemeshow dan uji diskriminasi dengan melihatnilai Area Under Curve. Hasil: Hasil analisis statistik menghasilkan bahwa skor P-POSSUM memiliki kemampuan kalibrasi yang baik (uji Hosmer-Lemeshow p=0.815) dan kemampuandiskriminasi yang cukup baik (AUC 77.8%, IK 95% 0.717-0.827). Variabel PPOSSUM yang secara statistik berpengaruh signifikan (p<0.05) terhadap lama perawatan di ICU adalah kadar natrium, jumlah perdarahan, laju jantung, dan EKG.Kesimpulan: Skor P-POSSUM sahih dalam memprediksi lama perawatan pasien pascabedah digestif mayor di ruang intensif (ICU).

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Background: It was estimated that there was 313 million surgery underwent worldwide, with the 30-days postoperative mortality rate reaching 4.2 million cases. The evaluation of preoperative patients’ conditions is encouraged to predict

postoperative morbidity and mortality, thus the modality used to assess surgery risk should be accurate and objective. RSUPN Cipto Mangunkusumo (RSUPNCM) routinely uses ASA-PS score to assess patients’ condition. Nonetheless, ASA-PS has

been regarded as subjective. Developed countries has started to replace this score with P-POSSUM score which was considered to be more accurate and objective. This study finds out the validity of P-POSSUM Score in predicting the length of

hospital stay in the ICU in patients who underwent digestive surgery, which reflects the severity of postoperative morbidity. Goals: This study investigated the calibration and discrimination ability of PPOSSUM score in predicting the length of stay in the ICU, and also explored the relationship between variables in P-POSSUM score and the length of stay in the ICU in patients who underwent digestive surgery.

Methods: This retrospective cohort study was conducted in RSUPNCM in January 2017 to December 2018 on 289 subjects who met the inclusion criteria. P-POSSUM score and the length of stay in the ICU unit were recorded, the data was taken from

medical record. Bivariate and multivariate logistic regression was used to investigate the relationship between P-POSSUM variables and the length of stay. The validity of P-POSSUM score was assessed by Hosmer-Lemeshow calibration

test and the measurement of the Area Under Curve (AUC).

Results: Statistical analysis showed that P-POSSUM had a good calibration ability (p=0.815 for Hosmer-Lemeshow test) and moderate discrimination ability (AUC 77.8%, CI 95% 0.717-0.827). Four P-POSSUM variables were found to be significantly associated with length of stay in the ICU (p<0.05), namely natrium level, total blood loss, heart rate and ECG. Conclusion: P-POSSUM score is valid in predicting the length of stay in the ICU in patients who underwent digestive surgery."

"Gangguan keseimbangan mikrobiota pada saluran pencernaan dapat menyebabkan dampak pada kesehatan. Upaya untuk menggunakan mikrobiota sebagai pengobatan dapat dilakukan salah satunya dengan memanfaatkan flora normal saluran pencernaan, sehingga meminimalkan kemungkinan gangguan yang tidak diharapkan. Penelitian sebelumnya telah berhasil mengisolasi beberapa bakteri dari mekonium neonatus, di antaranya Staphylococcus hominis, Bacillus subtilis, dan Enterococcus hirae. Penelitian ini bertujuan untuk mengeksplorasi potensi koktail ketiga bakteri untuk microbial therapeutic, dengan cara menguji daya saing koktail dengan tiga bakteri patogen saluran pencernaan, yaitu Salmonella typhi, Escherichia coli, dan Streptococcus mutans. Koktail bakteri dibuat dengan perbandingan Staphylococcus hominis : Bacillus subtilis :Enterococcus hirae 0,5 : 1 : 0,75 berdasarkan penelitian sebelumnya. Analisis daya saing dilakukan dengan Deferred Growth Inhibition Assay dan uji metode sumuran, agar kedua uji dapat saling mengkonfirmasi hasil analisis. Hasil uji DGIA dan metode sumuran menunjukkan adanya kemampuan koktail bakteri untuk bersaing dengan bakteri patogen berupa tidak terbentuknya zona penghambatan pertumbuhan satu dengan yang lain. Hasil metode sumuran terutama menunjukkan kemampuan koktail untuk bersaing yang tidak teramati pada bakteri individual berupa lingkaran jernih berbentuk cincin di sekitar sumuran.Hal ini menunjukkan potensi pengembangan lebih lanjut sebagai produk microbial therapeutic.

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Changesof the microbiota balance of the digestive system could impact health. One way to use microbial therapy is to use the commensal flora of the digestive system, minimalizing the chance of unwanted interactions. The previous study has isolatedseveral bacteria from neonatal meconium, i.e., Staphylococcus hominis, Bacillus subtilis, andEnterococcus hirae.This study explores the potential of the coktail of the three bacteria to be developed as a microbial therapeutic on the digestive systemby conducting an inhibition assay with three indicator bacteria of the pathogenic digestive system, i.e.,Salmonella typhi, Escherichia coli, andStreptococcus mutans.The cocktail was made based on a previous study with the composition ofStaphylococcus hominis : Bacillus subtilis :Enterococcus hirae,i.e.,0,5: 1: 0,75, respectively. The inhibition analysis was carried out by performing a Deferred Growth Inhibition Assay (DGIA)and well diffusion method assay so that both assays can confirm the results of each other. The results of DGIA and well method assayshow the ability of the cocktail to inhibit the pathogenic bacteria by the inhibition zone not being formed. The well method assay in particular shows the cocktail having inhibition ability not observed by individual bacteria by the clear ring zone around the wells. Thisshows further development potential of the cocktail as a microbial therapeutic product."