Hasil Pencarian  ::  Simpan CSV :: Kembali

"Black rice (Oryza sativa L. “Cempo Irengâ€) is one of local rice varieties in Sleman Regency, Yogyakarta. The black color is caused by high anthocyanin content which is important source of antioxidant. The cultivation of black rice is still limited due to its tall phenotype, long vegetative stage and low productivitycompared to white rice. Paclobutrazol is a growth retardant causing dwarfing in several crop plants and reducing lodging. Blue light can improve plant quality. This research was aimed to evaluate the effect of blue light and paclobutrazol on seed germination, vegetative growth and yield of black rice. The results showed that the average of seed germination as well as the activity of α-amylase of seeds subjected to blue light were lower compared to those subjected to sunlight; however, paclobutrazol concentrations did not affect seed germination percentage. The height of rice plants treated with paclobutrazol decreased in accordance with the increase of paclobutrazol concentration. Chlorophyll content and tiller numbers increased by paclobutrazol treatment of 12.5 ppm. Nitrate reductase activity was higher in rice seedlings subjected to blue light compared to those subjected to sunlight. Iron (Fe) content of rice plants treated with 25 or 50 ppm paclobutrazol increased compared to control. It was concluded thatpaclobutrazol applicationof 12.5 ppmalready reduced plant height. The higher concentration of paclobutrazol applied the greater reduction on plant height was observed.Blue light treatment applied during black rice seed germination slightly reduced germination percentage and α-amylase activity in the germinated seeds. However, blue light treatment combined with paclobutrazol application during black rice seed germination increased chlorophyll content, tiller numbers and Fe content in black rice grain."

"ABSTRAKLatar Belakang: Tiamin merupakan kelompok vitamin B yang dibutuhkan manusia namun harus diperoleh dari sumber luar. Tiamin memiliki banyak fungsi penting dalam tubuh, sehingga kekurangan tiamin dapat menyebabkan masalah serius. Pemeriksaan kadar tiamin dalam tubuh dengan metode yang tersedia saat ini membutuhkan biaya yang mahal. Berdasarkan hal tersebut, lahirlah ide untuk menggunakan prinsip ELISA dengan memanfaatkan protein pengikat tiamin dari sumber yang mudah diperoleh dan murah untuk skrining. Penelitian ini bertujuan untuk mengetahui adanya protein pengikat tiamin pada ketan hitam (Oryza sativa L.).Metode: Isolasi protein pengikat tiamin dari ketan hitam (Oryza sativa L.) dilakukan dengan metode salting out, dialisis, dan dialisis kesetimbangan. Salting out menggunakan konsentrasi garam amonium sulfat 90%.Hasil: Dalam penelitian ini, konsentrasi protein total adalah 5.190,48 g/mL setelah tahap salting out. Adanya protein pengikat tiamin dalam protein total dapat dibuktikan dengan dialisis ekuilibrium. Protein pengikat tiamin dari ketan hitam (Oryza sativa L.) dapat mengikat tiamin pada dialisis kesetimbangan 0,479 g/gram tepung.Kesimpulan: Ketan hitam (Oryza sativa L.) mengandung protein pengikat tiamin.ABSTRACTBackground: Thiamine is a group of B vitamins that humans need but must be obtained from external sources. Thiamine has many important functions in the body, so thiamine deficiency can cause serious problems. Examination of thiamine levels in the body with currently available methods is expensive. Based on this, the idea was born to use the ELISA principle by utilizing thiamine binding proteins from sources that are easily obtained and inexpensive for screening. This study aims to determine the presence of thiamine binding protein in black sticky rice (Oryza sativa L.).Methods: Isolation of thiamine binding protein from black sticky rice (Oryza sativa L.) was carried out by salting out, dialysis, and equilibrium dialysis methods. Salting out using a 90% concentration of ammonium sulfate salt.Results: In this study, the total protein concentration was 5,190.48 g/mL after the salting out stage. The presence of thiamine-binding protein in total protein can be demonstrated by equilibrium dialysis. Thiamine binding protein from black sticky rice (Oryza sativa L.) can bind thiamine at equilibrium dialysis of 0.479 g/gram flour.Conclusion: Black sticky rice (Oryza sativa L.) contains thiamine binding protein."

"Penelitian dilakukan untuk mengetahui pengaruh pemberian strain Nostoc terhadap pertumbuhan vegetatif dan generatif tanaman padi (Oryza sativa L.) varietas Ciherang. Penelitian menggunakan Rancangan Acak Lengkap dengan enam ulangan dan empat perlakuan. Perlakuan terdiri atas pemberian strain Nostoc BAD5, GIA13a, TAB7d, dan kontrol. Pemberian strain Nostoc dilakukan ketika padi berumur 15, 30, 45, dan 60 hari setelah tanam (hst). Biomassa berat basah strain Nostoc yang diberikan pada 15 dan 30 hst masing-masing sebesar 0,4 g dan biomassa berat basah strain Nostoc yang diberikan pada 45 dan 60 hst masingmasing sebesar 0,6 g. Hasil uji ANOVA (!= 0,05) menunjukkan bahwa pemberian strain Nostoc dapat menurunkan jumlah buah kosong. Hasil penelitian juga menunjukkan bahwa pemberian strain Nostoc dapat meningkatkan panjang akar dan jumlah buah isi (bernas), dengan uji Kruskal-Wallis (!= 0,05). Perlakuan GIA13a terbukti paling baik dalam menurunkan jumlah buah kosong, meningkatkan panjang akar dan jumlah buah isi, dengan uji LSD (!= 0,05).

---

The experiment aim was to investigate the effect of Nostoc strains to vegetative and generative growth of Ciherang varieties of rice (Oryza sativa L.). The experiment used Randomized Completely Design with six replications dan four treatments. The treatments were applied by giving Nostoc strains BAD5, GIA13a, TAB7d, and control. Nostoc strains were inoculated at 15, 30, 45, 60 days after plantation (hst). Total of 0,4 g Nostoc biomass was inoculated at 15 and 30 hst, while 0,6 g Nostoc biomass was inoculated at 45 and 60 hst. The results of ANOVA test (!= 0,05) showed that inoculated of Nostoc strains had effect to decrease the number of filledout grains. The result of this experiment also had effect to increase the root length and number of filled grains, by Kruskal-Wallis test (!= 0,05). Strain of GIA13a proven the best treatment to decrease number of filled-out grains, increase root length and number of filled grains, by LSD test (!= 0,05)."

"Penelitian ini menguji fitotoksisitas nanopartikel perak (NP Ag) hasil biosintesis pada perkecambahan padi dan jagung. Terdapat 5 kelompok perlakuan yaitu kontrol; NP Ag konsentrasi 5, 10, 15 mg/L; dan AgNO3 0,01 M. Paparan NP Ag dilakukan dengan merendam biji dalam larutan NP Ag selama 24 jam, lalu dikecambahkan selama 14 hari dalam kondisi gelap. Toksisitas NP Ag dianalisis dengan tiga parameter. Pertama, parameter perkecambahan terdiri dari daya kecambah (DK%), laju perkecambahan (LP), dan indeks kecepatan perkecambahan (IKP). Kedua, parameter biometrik dianalisis dengan mengukur panjang tunas dan akar; serta berat segar dan kering kecambah. Terakhir, parameter fisiologis yaitu kadar H2O2 daun. Hasil penelitian menunjukkan perlakuan NP Ag cenderung memiliki pengaruh yang tidak berbeda signifikan dengan kontrol dalam parameter perkecambahan, biometrik, dan fisiologis padi dan jagung. Namun, biji padi dan jagung dengan NP Ag 15 mg/L cenderung mengalami penurunan pada DK%, IKP, dan panjang tunas. Padi dengan perlakuan NP Ag 15 mg/L mengalami penurunan IKP yang berbeda signifikan dengan kontrol. Selain itu, terjadi penurunan berat segar seiring peningkatan konsentrasi NP Ag di padi dan jagung. Di sisi lain, diketahui kandungan H2O2 dalam padi yang terpapar NP Ag secara bertahap cenderung meningkat seiring peningkatan konsentrasi NP Ag, serta pada jagung dengan perlakuan NP Ag 15 mg/L memiliki kadar H2O2 yang cenderung lebih tinggi dari kontrol.

---

This study tested the phytotoxicity of biosynthetic silver nanoparticles (AgNPs) on rice and corn germination. There are 5 treatment groups, control; AgNPs concentration 5, 10, 15 mg/L; and 0,01 M AgNO3. Exposure to AgNPs was carried out by soaking the seeds in a solution of AgNPs for 24 hours, then germinated for 14 days in the dark. The toxicity of AgNPs was analyzed by three parameters. First, the germination parameters consisted of germination rate (DK%), germination rate (LP), and germination rate index (IKP). Second, biometric parameters were analyzed by measuring shoot and root length; and fresh and dry weight of sprouts. Last, the physiological parameter is the leaf H2O2 level. The results showed that the AgNPs treatment tended to have no significant effect with the control in germination, biometric, and physiological parameters of rice and corn. However, rice and corn seeds with AgNPs 15 mg/L tended to decrease in DK%, IKP, and shoot length. Rice treated with AgNPs 15 mg/L experienced a decrease in IKP which was significantly different from the control. In addition, there was a decrease in fresh weight as the concentration of AgNPs in rice and maize increased. On the other hand, it is known that the H2O2 content in rice exposed to AgNPs gradually tends to increase as the concentration of AgNPs increases, and corn with 15 mg/L AgNPs treatment has H2O2 levels which tend to be higher than the control."

"The polyamines such as putrescine, spermidine, and spermine were reported to increase green plant regeneration in rice anther culture. Low response of anther culture of rice sub-spicies indica may be improved with the addition of putrescine in the culture media..."

"Kekeringan adalah salah satu faktor stres abiotik yang mengurangi produktivitas padi di Indonesia. OsDREB2A adalah anggota subfamili DREB dari faktor transkripsi AP2/ERF dan berperan dalam mengatasi stres kekeringan dengan langsung mengikat elemen DRE untuk mengatur ekspresi gen di hilir. Namun, penelitian lebih lanjut masih diperlukan untuk mengamati setiap gen OsDREB2A pada varietas padi lokal Indonesia. Penelitian ini bertujuan untuk mengeksplorasi gen OsDREB2A pada beberapa varietas padi lokal Indonesia, yaitu dari Jawa (Ciherang, Situ Bagendid, Way Apo), Kalimantan (Beras Hitam), Aceh (Sigupai). DNA diisolasi dari daun masing-masing varietas, diamplifikasi menggunakan PCR, kemudian dielektroforesis dan disekuensing. Data sekuensing dianalisis menggunakan DNA Baser, BioEdit dan kemudian divisualisasikan menggunakan server SWISS-MODEL, Database Proyek Anotasi Genom Padi, dan alat peta kromosom. Hasil penelitian menunjukkan bahwa lima sampel memiliki cakupan query 100% dengan sekuens kultivar OsDREB2A Pokkali (KU159743.1), persentase identitas yang mirip 99,86% dibandingkan dengan kultivar R180 dan 99,62% dibandingkan dengan kultivar Nona Bokra. Perbedaan dalam struktur asam amino sembilan sampel dibandingkan dengan kultivar pembanding terletak pada panjang struktur ekor. Struktur asam amino masing-masing kultivar mengacu pada kromosom 1 pada lokus LOC_Os01g07120.

---

Drought is one of the abiotic stress factors that reduces rice productivity in Indonesia. OsDREB2A is a member of the DREB subfamily of AP2/ERF transcription factors and participates in drought stress by directly binding to DRE elements to regulate downstream gene expression. However, further research is still needed to observe each OsDREB2A gene in local Indonesian rice varieties. This research aims to explore the OsDREB2A gene in several local Indonesian rice varieties, namely from Java (Ciherang, Situ Bagendid, Way Apo), Kalimantan (Black Rice), Aceh (Sigupai). DNA was isolated from the leaves of each variety, amplified using PCR, and then electrophoresed and sequenced. Sequencing data were analyzed using DNA Baser, BioEdit and then visualized using the SWISS-MODEL server, Rice Genome Annotation Project Database, and chromosome map tools. The results showed that five samples had 100% query cover with the Pokkali OsDREB2A (KU159743.1) cultivar sequence, 99.86% similar percent identity compared to cultivar R180 and 99.62% similar percent identity compared to cultivar Nona Bokra. The difference in the amino acid structure of the nine samples compared to comparison cultivars lies in the length of the tail structure. The amino acid structure of each cultivar refers to chromosome 1 at the LOC_Os01g07120 locus."

"An optical modulation by the use of bending effect developed from the effect of relative bending loss on optical fiber. The bending loss will decrease if the bending angle decreases. By varying the bending angle the bending loss will vary too. The modulation process obtained through this mechanism. The bending angle on optical fiber obtained from three mandrel laid on the loud-speaker; two mandrel on the frame and one on the cone of loud-speaker, while optical fiber laid between the mandrel. If current signal injected on coil loudspeaker, the coil will push the mandrel and then push optical fiber to form bending angle. The obtained 3 dB frequency response ranged from 28 Hz to 5714.3 Hz while the SNR and modulation index are 32.98 dB and 0.956 respectively. This modulator would be useable for single channel instrumentation and control links."

"The ecotoxicological effects of Cr2+ on germination and early seedling growth of six pulses were investigated. Seeds of these plants were exposed to seven different concentrations of Cr (0-3.2 mM). The results indicated that root elongation and coleoptile growth of six pulse plants were more sensitive than seed germination for measurement of the toxic of Cr2+ pollutions. Different species show different levels of tolerance to Cr2+ pollution."