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"Pencemaran mikroplastik menjadi ancaman bagi kehidupan biota. Mikroplastik yang terakumulasi dalam saluran pencernaan biota dapat menghambat pertumbuhan, mempengaruhi reproduksi, dan kerusakan organ internal. Penelitian ini bertujuan untuk mengetahui kelimpahan dan  bentuk mikroplastik pada air, sedimen, insang, dan saluran pencernaan Ikan Nila Oreochromis niloticus di Situ Rawa Besar, Depok. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari situ. Sampel Ikan Nila diambil 5 ekor masing-masing pada 3 stasiun. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sampel diteteskan pada Sedgewick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian pada Juli 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 15,00-23,33 partikel L-1 pada air, 20.330,00-45.330,00 partikel kg-1 pada sedimen, 182,67-593,33 partikel ind-1 pada insang, dan 596,00-701,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada September 2023, yaitu berkisar antara 24,33-34,67 partikel L-1 pada air, 31.000,00-65.670,00 partikel kg-1 pada sedimen, 317,33-744,00 partikel ind-1 pada insang, dan 844,00-1.120,00 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fragmen pada air, Ikan Nila, dan sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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Microplastic pollution poses a threat to the life of aquatic organisms. Accumulated microplastics in the digestive tracts of these organisms can hinder growth, affect reproduction, and cause internal organ damage. This research aims to determine the abundance and forms of microplastics in water, sediment, gills, and digestive tracts of Nile Tilapia (Oreochromis niloticus) in Situ Rawa Besar, Depok. Water and sediment samples were collected from three sampling stations in the lake. Five Nile Tilapia samples were taken from each station. Gill and digestive tract samples were digested using 65% nitric acid (HNO3) solution. Twenty liters of water were collected and filtered using a plankton net. Sediment samples were dried in an oven, with 25 grams used for analysis. A saturated NaCl solution was employed to separate microplastics from impurities. One milliliter of the sample was dropped onto a Sedgewick Rafter Chamber, then observed and counted based on microplastic forms under a microscope. The research results in July 2022 indicated that microplastic abundance ranged from 15.00 to 23.33 particles L-1 in water, 20,330.00 to 45,330.00 particles kg-1 in sediment, 182.67 to 593.33 particles ind-1 in gills, and 596.00 to 701.33 particles ind-1 in the digestive tract. These abundances were lower than those in September 2023, ranging from 24.33 to 34.67 particles L-1 in water, 31,000.00 to 65,670.00 particles kg-1 in sediment, 317.33 to 744.00 particles ind-1 in gills, and 844.00 to 1,120.00 particles ind-1 in the digestive tract. The most dominant form of microplastics was fragments in water, Nile Tilapia, and sediment. This research indicates an increase in the quantity of microplastics in Situ Rawa Besar over time, supported by factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Ikan Nila Oreochromis niloticus (Linnaeus, 1758) pada Setu Babakan Jagakarsa, Jakarta Selatan banyak dikonsumsi oleh masyarakat sekitar. Penelitian ini bertujuan untuk menganalisis presentase kelimpahan bentuk mikroplastik pada insang, lambung, dan usus ikan Nila Oreochromis niloticus (Linnaeus, 1758) yang diperoleh dari Setu Babakan, Jagakarsa, Jakarta Selatan. Penelitian dilakukan di Setu Babakan dan Laboratorium Biologi Laut, Departemen Biologi FMIPA Universitas Indonesia. Sampel ikan Nila yang diteliti sebanyak 18 ekor dengan panjang berkisar 13-20 cm. Sampel diisolasi untuk kemudian dilakukan metode destruksi dengan menggunakan larutan HNO3 65% sehingga dapat dilakukan proses analisis kelimpahan dan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan terdapat total kelimpahan rata-rata pada sampel air permukaan sebesar 308,00 ± 128,29 partikel/liter; insang sebesar 7.820 ± 1.847 partikel/individu; pada sampel lambung sebesar 2.588,11 ± 751,33 partikel/individu; dan pada sampel usus sebesar 5.178,16 ± 2.098,55 partikel/individu. Total kelimpahan mikroplastik terbesar terdapat pada sampel insang dan terendah pada sampel lambung. Hasil uji statistik Kruskal-Wallis menunjukkan tidak ada perbedaan presentase bentuk mikroplastik (fiber, film, fragmen dan granula) yang signifikan pada sampel insang, lambung dan usus ikan Nila Oreochromis niloticus (Linnaeus,1758) di Setu Babakan Jagakarsa, Jakarta Selatan

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Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) in Setu Babakan Jagakarsa, South Jakarta is widely consumed by the local communities. This study analyzed percentage abudance of microplastics form in the gills, stomach, and intestinal of Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) from Setu Babakan Jagakarsa, South Jakarta. This study was conducted at Setu Babakan and Marine Biology Laboratory, Departement of Biology FMIPA UI, Depok. The samples of Nile Tilapia studied were 18 individuals with the same relative length between 13-20 cm. The sample were isolated in order to do the destruction methods with HNO3 65%, then analysis of abundance and shape of microplastics can be done. The results show that the average total abudance in the gills sample was 7.820 ± 1.847 particles/individual; in the stomach was 2.588,11 ± 751,33 particles/individual; and in the intenstine was 5.178,16 ± 2.098,55 particles/individuals. The highest total abudance of microplastics was found in the gills and the lowest in the stomach samples. The results from Kruskal-Wallis statistical test shows there was no significant difference in the percentage of microplastics form (fiber, film, fragment, and granules) in the gills, stomach, and intestinal samples of Nile Tilapia Oreochromis niloticus (Linnaeus,1758) in Setu Babakan, Jagakarsa, South Jakarta."

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Mikroplastik merupakan partikel plastik yang terdegradasi di lingkungan dengan ukuran < 5 mm. Kandungan mikroplastik di perairan tawar berpotensi membahayakan kelangsungan hidup biota di dalamnya. Penelitian ini bertujuan menganalisis kelimpahan dan persentase komposisi bentuk mikroplastik pada insang dan saluran pencernaan ikan nila Oreochromis niloticus (Linnaeus,1758) di Ciliwung daerah Srengseng Sawah, Jakarta Selatan. Insang dan saluran pencernaan (lambung dan usus) dari 10 sampel ikan nila diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO₃) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Hasil penelitian terdapat kelimpahan mikroplastik pada sampel insang, lambung, dan usus ikan nila diantaranya bentuk fiber, film, fragmen, dan granula. Total kelimpahan rata-rata mikroplastik di insang sebanyak 4.135 ± 3.297,20 partikel/ind, di lambung sebanyak 2.772 ± 1.030,43 partikel/ind, dan di usus sebanyak 3.598 ± 1.582,18 partikel/ind ditemukan pada ikan nila. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada ikan nila terdapat bentuk fiber sebesar 57,85% di insang; 53,21% di lambung dan 53,06% di usus.

 

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Microplastics are plastic particles that degrade in the environment with a size of <5 mm. The content of microplastics in freshwater has the potential to endanger the survival of the biota in it. This study aims to analyze the abundance and percentage composition of microplastic forms in the gills and digestive tract of Nile Tilapia fish Oreochromis niloticus (Linnaeus, 1758) in Ciliwung, Srengseng Sawah, South Jakarta. The gills and digestive tract (stomach and intestines) of 10 samples of nile tilapia fish were extracted and crushed using a 65% nitric acid (HNO3) solution. The extracted sample is then given a saturated NaCl solution so that the microplastic floats to the surface. The sample was dropped as much as 1 ml into the Sedgwick Rafter Chamber and then observed under a microscope and the number of microplastics was calculated based on the shape of the particles. The results showed an abundance of microplastics in the samples of gill, stomach, and intestines of tilapia including the form of fiber, film, fragments, and granules. The total abundance of microplastics in the gills was 4.135 ± 3.297,20 particles / ind, 2.772 ± 1.030,43 particles / ind in the stomach, and 3.598 ± 1.582,18 particles / ind in the intestine, which was found in tilapia. The percentage composition of the microplastic form that was found predominantly in tilapia contained a fiber form of 57,85% in the gills; 53,21% in the stomach and 53,06% in the intestine.

 

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"Mikroplastik yang mencemari perairan tawar dapat mengganggu keberlangsungan hidup biota di dalamnya. Penelitian ini bertujuan untuk menganalisis bentuk, warna, dan kelimpahan mikroplastik yang terkandung pada air, sedimen, insang, dan saluran pencernaan ikan red devil Amphilophus labiatus di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari kedua situ. Sampel ikan red devil diperoleh 15 ekor pada masing – masing situ. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sample diteteskan pada Sedgwick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan kelimpahan mikroplastik bentuk fiber, fragmen, film, granula terdapat pada seluruh sampel dengan warna yang beragam. Hasil penelitian menunjukkan total kelimpahan rata – rata mikroplastik di Situ Kenanga sebanyak 434,33 ± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel kg-1 pada sedimen, 268,33 ± 119,18 partikel gr-1 dan 1266,2 ± 349,72 partikel ind-1 pada insang, 287,79 ± 185,22 partikel gr-1 dan 978,22 ± 336,38 partikel ind-1 pada saluran pencernaan. Pada Situ Mahoni sebesar 437,67 ± 30,21 partikel L-1 pada air, 36.237,04 ± 16.702,59 partikel kg-1 pada sedimen, 429,18 ± 187,50 partikel gr-1 dan 1233,8 ± 253,60 partikel ind-1 pada insang, 318,04 ± 114,94 partikel gr-1 dan 1053,78 ± 328,44 partikel ind-1 pada saluran pencernaan.

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Microplastics that contaminate freshwater can disrupt the survival of the biota in it. This study aims to analyze the shape, color, and abundance of microplastics contained in water, sediment, gills, and digestive tract of red devil fish Amphilophus labiatus in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. Water and sediment samples were taken at 3 sampling stations from the two Situ. Samples of red devil fish were obtained 15 tails in each Situ. Samples of gills and digestive tract were destroyed using 65% nitric acid (HNO3) solution. Water samples were taken 20 L and filtered using a plankton net. Sediment samples were dried in an oven as much as 25 grams. Saturated NaCl solution was used to separate microplastics with impurities. A total of 1 mL of the sample was dropped into the Sedgwick Rafter Chamber and then observed and calculated based on the shape of the microplastic under a microscope. The results showed an abundance of microplastics in the form of fibers, fragments, films, and granules in all samples with various colors. The results showed the total average abundance of microplastics in Situ Kenanga was 434,33 ± 23,51 L-1 particles in water, 45.837,04 ± 36.305,97 particles kg-1 in sediments, 268.33 ± 119.18 particles gr-1 and 1266.2 ± 349.72 particles ind-1 in the gills, 287.79 ± 185.22 particles gr-1 and 978.22 ± 336.38 particles ind-1 in the digestive tract. At Situ Mahoni there are 437,67 ± 30,21 particles L-1 in water, 36.237,04 ± 16.702,59 particles kg-1 in sediments, 429.18 ± 187.50 particles gr-1 and 1233.8 ± 253.60 particles ind-1 on the gills, 318.04 ± 114.94 particles gr-1 and 1053.78 ± 328.44 particles ind-1 in the digestive tract.

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"Peningkatan jumlah limbah plastik yang dibuang ke Situ Rawa Besar mengancam habitat ikan cere. Limbah plastik akan terdegradasi menjadi partikel kecil yang disebut dengan mikroplastik. Mikroplastik dapat terkonsumsi oleh ikan cere karena ukurannya yang mirip dengan sumber makanannya. Penelitian ini bertujuan untuk mengetahui adanya keberadaan mikroplastik dan menganalisis berbagai macam bentuk mikroplastik pada air, sedimen dan ikan cere Gambusia affinis (Baird & Girard, 1853) di Situ Rawa Besar pada musim kemarau 2022 dan musim hujan 2023. Seluruh sampel diambil di 3 stasiun yaitu inlet, midlet, dan outlet. Sampel air 20 L disaring menggunakan plankton net 350 mesh, sampel sedimen diambil 250 mL menggunakan Ekman grab, ikan cere diambil secara acak menggunakan dip net sebanyak 15 individu. Sampel ikan diawetkan dengan alkohol 70% dan diekstraksi menggunakan 5mL HNO3 1 M. Sedimen dikeringkan pada oven 65C dan dihaluskan. Seluruh sampel dilarutkan dengan NaCl jenuh. Larutan dihomogenisasi sebanyak 20 mL dan 1 mL diteteskan pada Sedgewick Rafter Chamber untuk diamati di bawah mikroskop cahaya. Hasil pengamatan pada musim kemarau 2022 menunjukkan total rata-rata kelimpahan mikroplastik pada air sejumlah 46,33 ± 4,67 partikel L-1, pada sedimen sejumlah 31.777,78 ± 16.344,67 partikel kg-1, pada ikan cere sejumlah 291,11 ± 73,10 partikel ind-1 atau 93,52 ± 14,33 partikel cm-1. Sementara itu, pada musim hujan 2023 menunjukkan total rata-rata kelimpahan mikroplastik pada air sejumlah 64,67 ± 8,70 partikel L-1 , pada sedimen sejumlah 34.222,22 ± 17.493,91 partikel kg-1, dan pada ikan cere sejumlah 254,00 ± 40,28 partikel ind-1 atau 89,71 ± 12,15 partikel cm-1. Hasil Uji Korelasi Spearman menunjukkan terdapat korelasi positif sangat kuat antara kelimpahan mikroplastik pada air dengan sedimen, serta antara panjang ikan cere dengan kelimpahan mikroplastik pada ikan cere. Hasil analisis Uji T Dua Sampel menunjukkan tidak terdapat perbedaan nyata pada seluruh sampel.

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The increasing amount of plastic waste dumped into Situ Rawa Besar threatens the mosquitofish habitat. Plastic waste degrades into small particles called microplastics. Microplastics can be consumed by mosquitofish because their size is similar to that of their food source. This research aims to determine the presence of microplastics and analyze various forms of microplastics in water, sediment, and the mosquitofish Gambusia affinis (Baird & Girard, 1853) in Situ Rawa Besar during the 2022 dry season and the 2023 wet season. All samples were taken at three stations, namely inlet, midlet, and outlet. A 20 L water sample was filtered using a 350 mesh plankton net, 250 mL sediment samples were taken using an Ekman grab, and 15 mosquitofish were randomly captured using a dip net. Fish samples were preserved with 70% alcohol and extracted using 5 mL of 1 M HNO3. The sediment was dried in an oven at 65°C and ground. All samples were dissolved with saturated NaCl. The solution was homogenized to 20 mL, and 1 mL was dropped into the Sedgewick Rafter Chamber to be observed under a light microscope. The results of observations in the 2022 dry season showed that the total average abundance of microplastics in the water was 46.33 ± 4.67 particles L-1, in sediment was 31,777.78 ± 16,344.67 particles kg-1, and in mosquitofish was 291.11 ± 73.10 particles ind-1 or 93.52 ± 14.33 particles cm-1. Meanwhile, in the 2023 wet season, the total average abundance of microplastics in the water was 64.67 ± 8.70 particles L-1, in the sediment was 34,222.22 ± 17,493.91 particles kg-1, and in mosquitofish was 254.00 ± 40.28 particles ind-1 or 89.71 ± 12.15 particles cm-1. The results of the Spearman Correlation Test showed that there was a very strong positive correlation between the abundance of microplastics in water and sediment, as well as between the length of mosquitofish and the abundance of microplastics in mosquitofish. The results of the Two Sample T Test analysis show that there are no significant differences in all samples."

"Penelitian ini dilatarbelakangi oleh meningkatnya polusi plastik yang menyebabkan pencemaran mikroplastik di perairan air tawar. Situ Rawa Besar, Depok merupakan salah satu situ yang tercemar oleh mikroplastik akibat aktivitas manusia di sekitarnya. Mikroplastik dapat berdampak negatif bagi organisme akuatik seperti ikan sapu-sapu Pterygoplichthys pardalis yang merupakan spesies invasif dan bioindikator pencemaran mikroplastik di situ. Penelitian ini bertujuan untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu di Situ Rawa Besar serta menguji perbedaan kelimpahan mikroplastik pada tahun 2022 dengan 2023. Penelitian ini menggunakan metode deskriptif kuantitatif untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang, dan saluran pencernaan sapu-sapu di Situ Rawa Besar. Pengambilan sampel dilakukan di tiga stasiun. Parameter fisika-kimia air seperti suhu, pH, oksigen terlarut, dan kecerahan air diukur secara in situ. Sampel air 20 L disaring menggunakan plankton net dan sampel sedimen diambil menggunakan Ekman grab dan dikeringkan dengan oven, lalu ditambahkan larutan NaCl jenuh. Sampel ikan sapu-sapu diambil sebanyak 15 ekor dengan menggunakan jaring ikan. Sampel insang dan saluran pencernaan ikan dipisahkan dengan menggunakan dissecting set dan dilarutkan dengan HNO3 kemudian ditambahkan larutan NaCl jenuh. Perhitungan partikel mikroplastik dilakukan di bawah mikroskop dan sampel diletakkan pada Sedgwick Rafter Chamber. Mikroplastik dianalisis kelimpahannya dan diklasifikasikan menurut bentuknya (pellet, fiber, film, dan fragmen) serta dihitung persentase komposisi mikroplastik dalam sampel. Hasil penelitian pada tahun 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 12,67 – 20,33 partikel L-1 pada air, 14.400,00-38.400,00 partikel kg-1 pada sedimen, 250,67-386,67 partikel ind-1 pada insang, dan 313,33-369,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada tahun 2023, yaitu berkisar antara 14,67-32,00 partikel L-1 pada air, 16.533,33-41.600,00 partikel kg-1 pada sedimen, 333,33-446,67 partikel ind-1 pada insang, dan 429,33-502,67 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fiber pada air dan ikan sapu-sapu, fragmen pada sedimen. Warna mikroplastik yang paling dominan adalah biru pada air dan sedimen, hitam pada insang dan saluran pencernaan ikan sapu-sapu. Kelimpahan mikroplastik pada tahun 2023 lebih tinggi dibandingkan tahun 2022, dengan peningkatan sebesar 15,24% pada insang, 24,25% pada saluran pencernaan, 27,91% pada air, dan 7,12% pada sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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This research was motivated by the increasing plastic pollution that causes microplastic contamination in freshwater bodies. Situ Rawa Besar, Depok is one of the lakes that is polluted by microplastics due to human activities around it. Microplastics can have negative impacts on aquatic organisms such as Amazon Sailfin Catfish Pterygoplichthys pardalis which is an invasive species and a bioindicator of microplastic pollution in the lake. This research aims to analyze the abundance, shape and color of microplastics in water, sediment, gills and digestive tract of sailfin catfish in Situ Rawa Besar and to test the difference in microplastic abundance between 2022 and 2023. This research uses descriptive quantitative method to analyze the abundance, shape and color of microplastics in water, sediment, gills, and digestive tract of sailfin catfish in Situ Rawa Besar. Sampling was done at three stations. Physico-chemical parameters of water such as temperature, pH, dissolved oxygen, and water clarity were measured in situ. Water samples of 20 L were filtered using plankton net and sediment samples were taken using Ekman grab and dried with oven, then added with saturated NaCl solution. Sailfin catfish samples were taken as many as 15 individuals using fish net. Gill and digestive tract samples were separated using dissecting set and dissolved with HNO3 then added with saturated NaCl solution. Microplastic particle counting was done under microscope and samples were placed on Sedgwick Rafter Chamber. Microplastics were analyzed for their abundance and classified according to their shape (pellet, fiber, film, and fragment) and the percentage composition of microplastics in the sample was calculated. The results of the research in 2022 showed that the abundance of microplastics ranged from 12.67-20.33 particles L-1 in water, 14,400.00-38,400.00 particles kg-1 in sediment, 250.67-386.67 particles ind-1 in gills, and 313.33-369.33 particles ind-1 in digestive tract. The abundance of microplastics was lower than its abundance in 2023, which ranged from 14.67-32.00 particles L-1 in water, 16,533.33-41,600.00 particles kg-1 in sediment, 333.33-446.67 particles ind-1 in gills, and 429.33-502.67 particles ind-1 in digestive tract. The most dominant shape of microplastics was fiber in water and sailfin catfish, fragment in sediment and digestive tract of sailfin catfish. The most dominant color of microplastics was blue in water and sediment, black in gills and digestive tract of sailfin catfish. The abundance of microplastics increased from 2022 to 2023, with an increase of 15.24% in gills, 24.25% in digestive tract, 27.91% in water, and 7.12% in sediment. This research shows an increase in the number of microplastics in Situ Rawa Besar over time supported by several factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Keberadaan mikroplastik di perairan dapat mengancam biota di dalamnya. Penelitian dilakukan dengan tujuan menganalisis kelimpahan, bentuk, dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu Pterygoplichthys pardalis dari Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air dan sedimen dilakukan pada inlet, midlet, dan outlet kedua situ. Sampel air disaring menggunakan plankton net, sampel sedimen diambil menggunakan Ekman grab kemudian dikeringkan menggunakan oven, sampel P. pardalis sebanyak 15 individu diambil menggunakan cast net dari masing-masing situ, kemudian insang dan saluran pencernaannya diisolasi dan didestruksi menggunakan HNO3 65%. Tiap sampel yang diperoleh dilarutkan dengan larutan NaCl jenuh agar terjadi flotasi. Pengamatan dilakukan menggunakan mikroskop cahaya dan Sedgwick Rafter Chamber untuk meletakkan sampel, dengan mengamati bentuk, warna, dan jumlah partikel mikroplastik. Hasil penelitian menunjukkan bahwa bentuk fiber, film, fragmen, granula, serta warna transparan, hitam, biru, dan merah muda ditemukan pada semua sampel. Kelimpahan mikroplastik di Situ Kenanga pada air sebanyak 48,26 ± 23,51 partikel L-1, sedimen 45837,04 ± 36305,97 partikel Kg-1, insang 290,48 ± 154,58 partikel g-1 atau 1156,44 ± 378,69 partikel ind-1, saluran pencernaan 134,37 ± 55,72 partikel g-1 atau 1364,89 ± 339,54 partikel ind-1. Kelimpahan mikroplastik di Situ Mahoni pada air sebanyak 48,63 ± 30,21 partikel L-1, sedimen 36237,04 ± 16702,60 partikel Kg-1, insang 287,23 ± 109,40 partikel g-1 atau 1153,78 ± 324,32 partikel ind-1, saluran pencernaan 123,77 ± 34,35 partikel g-1 atau 1304,44 ± 270,90 partikel ind-1. Tidak terdapat perbedaan signifikan antara kelimpahan mikroplastik di Situ Kenanga dan Situ Mahoni pada semua sampel.

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The presence of microplastics in the water could threaten the biota there. This study was conducted to analyze the abundance, shapes, and colors of microplastics in water, sediment, gills and digestive tract of amazon sailfin catfish Pterygoplichthys pardalis from Situ Kenanga and Situ Mahoni, Universitas Indonesia Campus, Depok. Sampling of water and sediment were carried out at the inlet, midlet, and outlet of both situ. Water samples were filtered using plankton net, sediment samples were taken using Ekman grab and dried using an oven, as many as 15 individual P. pardalis samples were taken using cast net from each situ, then their gills and digestive tract were isolated and pulverized using 65% HNO3. Each sample obtained was dissolved with saturated NaCl solution for flotation to occur. Observations were made using a light microscope and Sedgwick Rafter Chamber to place each sample, by observing the shape, color, and number of microplastic particles. The results showed that the shapes of fibers, films, fragments, granules, as well as transparent, black, blue, and pink colors were found in all samples. The abundance of microplastics in Situ Kenanga water was 48.26 ± 23.51 particles L-1, sediment 45837.04 ± 36305.97 particles Kg-1, gills 290.48 ± 154.58 particles g-1 or 1156, 44 ± 378.69 ind-1 particles, digestive tract 134.37 ± 55.72 particles g-1 or 1364.89 ± 339.54 ind-1 particles. The abundance of microplastics in Situ Mahoni water was 48.63 ± 30.21 particles L-1, sediment 36237.04 ± 16702.60 particles Kg-1, gills 287.23 ± 109.40 particles g-1 or 1153, 78 ± 324.32 ind-1 particles, digestive tract 123.77 ± 34.35 particles g-1 or 1304.44 ± 270.90 ind-1 particles. There was no significant difference between the abundance of microplastics in Situ Kenanga and Situ Mahoni in all samples."

"Mikroplastik didefinisikan sebagai partikel plastik dengan ukuran <5 mm. Mikroplastik dapat masuk ke dalam tubuh organisme air khususnya ikan melalui oral dan insang. Penelitian ini bertujuan untuk mengetahui bentuk dan kelimpahan mikroplastik pada air, insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) serta menguji perbedaan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis Universitas Indonesia. Penelitian dilakukan di Departemen Biologi FMIPA UI, Depok. Sampel ikan mujair diperoleh sebanyak total 20 ekor dari kedua danau dengan panjang tubuh relatif antara 16--19 cm. Insang dan saluran pencernaan dari 20 sampel ikan mujair diisolasi, kemudian diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Total kelimpahan rata-rata mikroplastik pada air Danau Kenanga sebesar 1.766,6 ± 40,11 partikel/L, sementara total kelimpahan rata-rata mikroplastik air pada Danau Agathis sebesar 1.885,53 ± 106,27 partikel/L. Total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Kenanga di insang sebanyak 6.232 ± 1.898,66 partikel/ind dan di saluran pencernaan sebanyak 9.108 ± 4.027,14 partikel/ind, sementara total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Agathis di insang sebanyak 6.716 ± 2.467,67 partikel/ind dan di saluran pencernaan sebanyak 4.038 ± 2.180,75 partikel/ind. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Kenanga terdapat bentuk fragmen sebesar 40% di air; fiber 80% di insang dan fiber 75% di saluran pencernaan, Sementara komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Agathis terdapat bentuk fiber sebesar 43% di air; 75% di insang dan 67% di saluran pencernaan. Hasil analisis statistik Uji Mann-Whittney menunjukkan adanya perbedaan secara signifikan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis, Universitas Indonesia.

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Microplastics are define as plastic particles with a size of <5 mm. Microplastics can enter the body of aquatic organisms, especially fish through the mouth and gills. This study aims to determine the shape and abundance of microplastics in water, gill and digestive tract tilapia fish Oreochromis mossambicus (Peters, 1852) at Kenanga Pond and Agathis Pond, University of Indonesia and to analyze and examine differences in the abundance of microplastics in tilapia fish at Kenanga Pond and Agathis Pond, University of Indonesia. The study was conducted at Department of Biology FMIPA UI, Depok. Total 20 tilapia fish from the two lakes with relative length between 16--19 cm. Gills and digestive tract samples were obtained of tilapia fish were isolated, extracted and then crushed using a saturated solution of nitric acid (HNO3) 65%. The extracted sample is then given NaCl solution so that the microplastic floats to the surface. As much as 1 ml NaCl were dropped in the Sedgwick Rafter Chamber then observed under a microscope. The number of microplastic was calculated based on the type. The average total abundance of microplastics in the water of Kenanga Pond was 1.766,6 ± 40,11 particles/L, meanwhile the average total abundance of microplastics in the water of Agathis Pond was 1.885,53 ± 106,27 particles/L. The average total microplastics in tilapia gill at Kenanga Pond was 6.232 ± 1.898,66 particles/ind and in the digestive tract was 9.108 ± 4,027.14 particles/ind, meanwhile the average total abundance microplastics in tilapia gill at Agathis Pond was 6.716 ± 2.467,67 particles/ind and in the digestive tract was 4.038 ± 2.180,75 particles/ind. The percentage composition of the microplastic form that was found dominant at Kenanga Pond were as much as 40% fragments in the water; 80% fiber in the gills and 75% fiber in the digestive tract. Meanwhile, the percentage composition of the microplastic form that was found dominant at Agathis Pond were as much as 43% fiber in the water; 75% in the gills and 67% in the digestive tract. The results of the Mann-Whittney test statistical analysis showed that there was significant difference between abundance of microplastics in tilapia fish at of Kenanga Pond and Agathis Pond, University of Indonesia."

"Penelitian ini bertujuan untuk mengetahui kelimpahan mikroplastik pada air, sedimen, serta insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) dan Ikan Sepat Rawa Trichopodus trichopterus (Pallas, 1770) di Situ Mahoni, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L menggunakan beaker glass 1000 mL lalu disaring menggunakan plankton net hingga tertampung volume air 300 mL, sampel sedimen diambil menggunakan ekman grab dan ditampung pada jar kaca 250 mL. Sementara itu, sampel ikan mujair dan ikan sepat rawa masing-masing diambil 15 ekor dengan cast net lalu disimpan pada wadah penyimpanan berisi alkohol 70%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode penghancuran oleh HNO3 65% untuk sampel ikan. Sedangkan, sampel sedimen dikeringkan dengan menggunakan oven selama 2 hari. Selanjutnya, seluruh sampel dicampurkan dengan larutan NaCl jenuh untuk mengapungkan mikroplastik. Kemudian dilakukan analisis kelimpahan, bentuk, dan warna mikroplastik menggunakan mikroskop. Hasil penelitian menunjukkan rata-rata total kelimpahan mikroplastik pada air diperoleh 437,67 ± 30,21 partikel L-1, pada sedimen diperoleh 36.237,04 ± 16.702,60 partikel kg-1, pada insang ikan mujair diperoleh 785,78 ± 292,07 partikel ind-1, pada insang ikan sepat rawa diperoleh 553,33 ± 242,54 partikel ind-1, saluran pencernaan ikan mujair diperoleh 1.058,67 ± 215,44 partikel ind-1, pada saluran pencernaan ikan sepat rawa diperoleh 892,89 ± 156,52 partikel ind-1. Secara keseluruhan, bentuk mikroplastik yang paling banyak ditemukan adalah mikroplastik berbentuk fiber. Berdasarkan hasil uji Mann-Whitney terhadap sampel insang dan saluran pencernaan dari ikan mujair dan ikan sepat rawa didapatkan bahwa terdapat perbedaan jumlah mikroplastik yang signifikan.

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The purpose of this study is to identify the abundance of microplastics in water, sediment, gills, and digestive tract of tilapia Oreochromis mossambicus (Peters,1852) and three spot gourami Trichopodus trichopterus (Pallas, 1770) in Situ Mahoni, University of Indonesia Campus, Depok. As much as 20 Litres of water samples were taken using a 1000 mL beaker glass and then filtered using a plankton net until 300 mL of water is stored, Sediment samples were taken using an ekman grab then stored on a 250 mL glass jar, while 15 samples of the tilapia and three spot gourami were caught using a cast net and then stored with alcohol 70%. Sample extractions were carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI by pulverizing the fish samples with HNO3 65%. The sediment samples were dried using the oven for 2 days. Then, all of the samples were mixed with a NaCl solution to float the microplastics. Then, an analysis of abundance, shape, and colours of microplastics under the microscope were done. The results showed that the average total abundance of microplastics was 437,67 ± 30,21 L-1 particles in water samples, in sediment samples was 36.237,04 ± 16.702,60 kg-1 particles in sediment samples, 785,78 ± 292,07 ind-1 particles in the gills of tilapia, 553,33 ± 242,54 ind-1 particles in the gills of the three spot gourami, 1.058,67 ± 215,44 ind-1 particles in the digestive tract of the tilapia, 892,89 ± 156,52 ind-1 particles in the digestive tract of the three spot gourami. Overall, the predominant form of microplastic in the water, sediment, gills, and digestive tract is in the form of fiber. There is a significant difference between the abundance of microplastics based on the results of the Mann-Whitney Test on gill and digestive tract samples of tilapia and three spot gourami."

"Telah dilakukan penelitian yang bertujuan untuk menganalisis bentuk dan kelimpahan mikroplastik pada air, insang, dan saluran pencernaan ikan mujair Oreochromis mossambicus dan ikan setan merah Amphilophus labiatus yang terdapat di Situ Salam, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L lalu disaring menggunakan plankton net sampai volume air menjadi 200 ml sementara sampel ikan mujair dan ikan setan merah diambil masing-masing 10 ekor dengan jala lalu disimpan di wadah penyimpanan berisi formalin 40%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode dekstruksi oleh HNO3 65% kemudian dilakukan analisis bentuk dan kelimpahan mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan, rata-rata total kelimpahan mikroplastik pada air diperoleh 153,7 ± 44,2 partikel L-1, pada saluran pencernaan ikan mujair diperoleh 2.868 ± 723,5 partikel ind-1, pada saluran pencernaan ikan setan merah diperoleh 3.548,4 ± 1031,4 partikel ind-1, pada insang ikan mujair diperoleh 3.782,6 ± 1.171,6 partikel ind-1, dan pada insang ikan setan merah diperoleh 3.848 ± 863,1 partikel ind-1. Bentuk mikroplastik yang mendominasi pada air situ, saluran pencernaan, dan insang adalah bentuk fiber. Berdasarkan hasil Uji T Dua Sampel terhadap sampel insang dan pencernaan dari ikan mujair dan ikan setan merah menunjukkan tidak ada perbedaan signifikan.

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Research has been carried out that aims to analyze the shape and microplastics in the water, gills, and digestive tract of tilapia fish Oreochromis mossambicus and red devil fish Amphilophus labiatus that found in Salam Lake, University of Indonesia Campus, Depok. Water samples were taken as much as 20 L and then filtered using a plankton net until the water volume became 200 ml while samples of tilapia fish and red devil fish were taken each with a net and stored in a storage container containing formalin 40%. Sample extraction was carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI with the destruction method by HNO3 65% and then analyzed the shape and abundance of microplastics under a microscope. The results showed that the average total abundance of microplastics in water was 153,7 ± 44,2 L-1 particles, in the digestive tract of tilapia fish it was obtained 2.868 ± 723,5 ind-1 particles, in the digestive tract of red devil fish obtained 3.548,4 ± 1.031,4 ind-1 particles, in the gills of tilapia fish obtained 3.782,6 ± 1.171,6 ind-1 particles, and in the gills of red devil fish obtained 3.848 ± 863,1 particles ind-1. The predominant form of microplastic in the water, digestive tract, and gills is the form of fiber. Based on the results of the Two-Sample T-Test on gill and digestive samples of tilapia fish and red devil fish, there is no significant difference."