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Natasya Prameswari
"Latar Belakang. Preeklamsia merupakan salah satu komplikasi kehamilan yang menyebabkan angka kematian ibu di Indonesia. Kehamilan dengan preeklamsia diduga berhubungan dengan kejadian stress oksidatif yang hingga kini etiologinya masih merupakan perdebatan. Pendekatan secara metabolomik dengan pemeriksaan asam amino serum maternal diharapkan nantinya dapat memberikan penjelasan mengenai etiologi dari preeklamsia.
Tujuan. Mendapatkan perbandingan antara kadar asam amino pada pasien preeklamsia dibandingkan kehamilan normal.
Metode. Penelitian ini merupakan penelitian deskriptif analitik, menggunakan 30 serum maternal wanita dengan kehamilan normal dan 34 preeklamsia berat yang kemudian dilakukan pemeriksaan dengan  instrumen LCMS/MS. Hasil penelitian kemudian dilakukan analisis dengan SPSS ver 20.0. Dilakukan pemeriksaan 19 variabel asam amino esensial maupun non-esensial. Kadar fenilalanin lebih tinggi pada pasien preeklamsia, dan metionin lebih rendah pada pasien preeklamsia. Sedangkan pada asam amino non-esensial serin, glisin, dan glutamat ditemukan lebih tinggi pada pasein preeklamsia.
Kesimpulan. Fenilalanin, serin, glisin, dan glutamat meningkat pada pasien preeklamsia, sedangkan metionin lebih rendah dibandingkan pasien kehamilan normal. Keseluruhan gangguan kadar asam amino yang meningkat pada pasien preeklamsia dapat merupakan sebuah dugaan bahwa hal tersebut terkait dengan adanya stress oksidatif yang terjadi pada pasien preeklamsia. Data dasar tersebut dapat dipikirkan untuk menjadi basis untuk penelitian selanjutnya

Background: Preeclampsia has been one of the leading cause of death in Indonesia. It is postulated that its relationship with oxidative stress may be the grounds of the disease.  Metabolomics approach to check the levels of amino acid are hope to give initial explanation related to preeclampsia.
Aim: LCMS/MS instrument was used in maternal serum of 30 cases of normal pregnancy and 34 cases of preeclampsia, these cases were then analyzed statistically
Results: We identified 19 different levels of aminoacids in this study. Four amino acids that were elevated in this study were phenylalanine, serine, glycine, and glutamate. Methionine were lower in preeclamsia
Conclusion: Phenylalanine, serine, glycine, and glutamate were elevated in preeclampsia, and methionine were lower in prreeclampsia. Abnormalities on amino acid levels were thought to be an evidence of oxidative stress that were related with preeclampsia
"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2020
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UI - Tugas Akhir  Universitas Indonesia Library
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Lingga Yudistira Abral
"Latar Belakang: Intrauterine Growth Restriction (IUGR) merupakan masalah yang sangat lazim pada kehamilan di dunia. Selain insufisiensi plasenta, penyebab lain adalah defisiensi nutrisi. Pada negara berkembang seperti Indonesia, konsumsi karbohidrat tetap tinggi sedangkan protein masih rendah. Asam amino sebagai komponen dasar dari setiap protein sangat berperan dalam pertumbuhan janin. Defisiensi asam amino pada akhirnya akan meningkatkan prevalensi IUGR. Namun, hanya ada beberapa penelitian tentang dampak masing-masing asam amino pada IUGR. Penelitian ini bertujuan untuk mengetahui kadar asam amino serum ibu pada IUGR dan kehamilan normal.
Metode: Penelitian observasional analitik dengan desain cross sectional dilakukan pada ibu hamil tunggal yang menjalani pemeriksaan kehamilan trimester III di RSUD dr. Rumah Sakit Umum Pusat Nasional Cipto Mangunkusumo, Indonesia selama periode September 2019 sampai Januari 2020. Subyek dengan IUGR atau kehamilan normal dimasukkan. Subyek dengan preeklamsia, diabetes gestasional, intervensi nutrisi, infeksi kronis, perokok, atau kelainan kongenital janin dieksklusikan. Berbagai kadar asam amino esensial dan non esensial ditentukan dari masing-masing subjek.
Hasil: Sebanyak 50 subjek (30 normal dan 20 IUGR) direkrut untuk penelitian. Tingkat asam amino ibu yang lebih tinggi pada kehamilan IUGR diamati pada valin, metionin, isoleusin, leusin, dan sistein (p <0,05) sedangkan tingkat yang lebih rendah diamati pada arginin, aspartat, dan serin (p <0,05).
Kesimpulan: Beberapa asam amino esensial kecuali arginin lebih tinggi pada IUGR, sedangkan asam amino non-esensial kecuali sistein lebih rendah pada IUGR.

Background: Intrauterine growth restriction (IUGR) is a highly prevalent problem on pregnancies in the world. Besides placental insufficiency, other etiology is nutritional deficiency. On developing countries such as Indonesia, consumption of carbohydrate remains high while protein remains low. Amino acid as the basic component of every protein highly contributes to fetal growth. Deficiency of amino acids would eventually increase the prevalence of IUGR. However, there is only a few studies on the impact of each amino acids on IUGR. This study aims to determine the level of maternal serum amino acids on IUGR and normal pregnancy.
Methods: An analytic observational study using cross sectional design was done on women with singleton pregnancy who was having third trimester antenatal care at dr. Cipto Mangunkusumo National General Hospital, Indonesia during the period of September 2019 to January 2020. Subjects with IUGR or normal pregnancy were included. Subjects with preeclampsia, gestational diabetes, nutrition intervention, chronic infections, smoker, or fetal congenital anomaly were excluded. Various essential and non-essential amino acids level were determined from each subject.
Results: A total of 50 subjects (30 normal and 20 IUGR) was recruited to the study. Higher maternal amino acids level in IUGR pregnancy was observed on valine, methionine, isoleucine, leucine, and cysteine (p < 0.05) while lower level was observed on arginine, aspartate, and serine (p < 0.05).
Conclusion: Some amino acids essential except arginine were higher in IUGR, whereas non-essential amino acids except cysteine was lower in IUGR.
"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
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UI - Tugas Akhir  Universitas Indonesia Library
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Hilya Auliy
"Pada penelitian ini, asam risinoleat diesterifikasi dengan dry metanol dan katalis KOH dengan sistem reflux. Metil risinoleat yang terbentuk dioksidasi pada ikatan rangkapnya membentuk diol menggunakan KMnO4 encer dalam suasana basa pada suhu 0oC. Metil risinoleat kemudian diamidasi menggunakan asam amino glisin dan asam amino fenilalanin untuk menghasilkan senyawa lipoamida. Hasil karakterisasi lipoamida yang terbentuk menggunakan FTIR menunjukkan adanya pita serapan ulur N-H dan O-H yang overlaping pada bilangan gelombang 3445,47 cm-1 untuk lipoamida glisin-risinoleat dan 3434,06 cm-1 untuk lipoamida fenilalanin-risinoleat. Selain itu, muncul puncak serapan medium vibrasi C-N pada bilangan gelombang 1217,90 cm-1 pada lipoamida glisin-risinoleat dan 1217,59 cm-1 pada lipoamida fenilalanin-risinoleat. Hal ini menunjukkan ikatan amida yang terbentuk dari proses amidasi. Hasil uji sitotoksik MTT senyawa lipoamida terhadap sel HeLa menunjukkan bahwa nilai IC50 lipoamida glisin-risinoleat sebesar 120 µg/mL yang termasuk ke dalam kategori cukup aktif, sedangkan IC50 lipoamida fenilalanin-risinoleat sebesar 250 µg/mL yang tergolong memiliki sifat sitotoksisitas yang lemah terhadap sel HeLa.

In this study, ricinoleic acid from castor oil was esterified with dry methanol and KOH catalyst using the reflux system. The methyl ricinoleate formed was oxidized on its double bonds to form a diol using dilute KMnO4 under alkaline conditions at 0oC. Methyl ricinoleate was then reacted through amidation process using amino acid glycine and amino acid phenylalanine to produce lipoamides. The results of characterization of lipoamides formed using FTIR showed that there were overlapping N-H and O-H stretch bands at wave numbers 3445.47 cm-1 for glycine-ricinoleate lipoamide and 3434.06 cm-1 for phenylalanine-ricinoleate lipoamide. In addition, the medium absorption peak of C-N appeared at the wave number 1217.90 cm-1 for glycine-ricinoleate lipoamide and 1217.59 cm-1 for phenylalanine-ricinoleate lipoamide. These showed that the amide bonds were formed from the amidation process. The results of the MTT cytotoxic assay of lipoamide compounds against HeLa cells showed that the IC50 value of glycine-ricinoleate lipoamide was 120 µg / mL which was considered quite active, while the IC50 value of phenylalanine-ricinoleate lipoamide was 250 µg / mL which was classified as having weak cytotoxicity properties against HeLa cells"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2020
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UI - Skripsi Membership  Universitas Indonesia Library
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Siahaan, Charly Haposan Marulitua
"Latar Belakang: Asam lemak rantai panjang Omega-3 dan Omega-6 selain penting untuk perkembangan otak janin, diketahui juga dapat mempengaruhi kejadian preeklamsia. Derivate omega-3 seperti DHA dapat membantu stimulasi Vascular Endothelial Growth Factor (VEGF) yang berperan dalam angiogenesis saat implantasi trofoblas. Belum tersedianya data pengaruh asam lemak rantai panjang terhadap kejadian preeklamsia mendorong untuk dilakukannya penelitian ini.
Tujuan: Mengetahui perbedaan kadar Omega-3 dan Omega-6 pada ibu hamil dengan preeklamsia dan normal.
Metode: Penelitian dilakukan dengan uji potong-lintang menggunakan sampel ibu hamil preeklamsia dan tidak preeklamsia yang telah dikumpulkan sebelumnya pada tahun 2018 di RSUPN Dr. Cipto Mangunkusumo dan RSU Budi Kemuliaan  dan RSUD Koja, kemudian dilakukan pengolahan pada Januari 2020.
Hasil: Diperoleh 60 subjek penelitian dengan 30 subjek pada masing-masing kelompok. Hasil dengan kategori rendah didapatkan pada kelompok preeklamsia dengan median kadar Omega-3, ALA 39 µmol/L, EPA 9 µmol/L dengan perbedaan yang bermakna (p=0,01). Sedangkan kadar  Omega-6 yang tinggi pada preeklamsia, LA 4726 µmol/L dan AA 558 µmol/L, menunjukkan asam lemak tersebut merupakan pro inflamasi. Indeks omega-3 pada kelompok preeklamsia juga rendah yaitu 2%, begitu pula rasio Omega-6/Omega-3 yang tinggi (17 µmol/L) dan rasio AA/EPA 61µmol/L pada kelompok preeklamsia sesuai dengan reaksi pro inflamasi pada preeklamsia 
Kesimpulan: Rendahnya kadar Omega-3, tingginya kadar Omega-6, indeks Omega-3 rendah, rasio Omega-6/Omega-3 dan rasio AA/EPA yang tinggi didapatkan pada preeklamsia.

Background: Omega-3 and Omega-6 long-chain fatty acids, besides being important for fetal brain development, are also known to influence the incidence of preeclampsia. Omega-3 derivatives such as DHA can help stimulate Vascular Endothelial Growth Factor (VEGF) which plays a role in angiogenesis during trophoblast implantation. The unavailability of data on the effect of long-chain fatty acids on the incidence of preeclampsia is encouraging this study.
Objective: To determine differences in levels of Omega-3 and Omega-6 in pregnant women with preeclampsia and normal
Methods: The study was carried out by cross-sectional testing using samples of preeclampsia and non-preeclampsia pregnant women that had been collected earlier in 2018 at Dr. RSUPN. Cipto Mangunkusumo and Budi Kemuliaan Hospital and Koja District General Hospital then processed in January 2020.
Results: Obtained 60 research subjects with 30 subjects in each group. Results with low category were obtained in the preeclampsia group with median levels of Omega-3, ALA 39 μmol/L, EPA 9 μmol/L with significant differences (p = 0.01). While high levels of Omega-6 in preeclampsia, LA 4726 μmol/L and AA 558 μmol/L. The omega-3 index in the preeclampsia group was also low at 2%, as was the high Omega-6/Omega-3 ratio (17 μmol/L) and the 61 μmol/L AA/EPA ratio in the preeclampsia group. Conclusion: An imbalance between Omega-3 and Omega-6, low index of Omega-3, a ratio of Omega-6/Omega-3, and ratio of high AA/EPA is found in preeclampsia.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2020
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UI - Tugas Akhir  Universitas Indonesia Library
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Anak Agung Eka Widya Saraswati
"Penelitian ini bertujuan untuk mengetahui asupan asam amino taurin dan korelasinya dengan aktivitas superoksida dismutase pada darah pasien osteoartritis lutut. Pada osteoartritis terjadi ketidakseimbangan antara prooksidan dengan antioksidan sehingga menimbulkan keadaan yang disebut stres oksidatif. Antioksidan enzimatik superoksida dismutase berperan dalam mencegah terjadinya stres oksidatif dengan cara memutus reaksi berantai radikal bebas sejak awal. Superoksida dismutase bekerja dengan cara mengkatalisis superoksida menjadi hidrogen peroksida. Pada osteoartritis diketahui terjadi peningkatan superoksida dan penurunan aktivitas superoksida dismutase. Asam amino taurin merupakan asam amino yang terdapat dalam jumlah tinggi di tubuh namun tidak ikut berperan serta dalam sintesis protein. Asam amino taurin banyak terdapat dalam bahan makanan sumber protein hewani terutama ikan, daging dan hasil laut. Asam amino taurin mempunyai beberapa sifat antara lain sebagai antioksidan, antiinflamasi, dan kondroprotektif. Penelitian ini menggunakan disain potong lintang dengan melibatkan 56 subjek OA lutut yang direkrut melalui consecutive sampling. Asupan taurin diambil dengan metode FFQ semikuantitatif. Sampel aktivitas superoksida dismutase diambil dari darah dan diukur menggunakan RANSOD SD 125 dengan metode spektrofotometri. Uji statistik menggunakan uji korelasi dengan SPSS. Rerata usia adalah 50,75 6,17 tahun, sebanyak 89,3 berjenis kelamin perempuan. Median asupan asam amino taurin adalah 59,77 15,96-278,57 mg per hari. Median aktivitas superoksida dismutase adalah 274,97 152,48-360,97 unit/mL dan didapatkan sebanyak 64,3 subjek dengan aktivitas superoksida dismutase yang meningkat. Pada penelitian ini didapatkan korelasi positif bermakna dengan kekuatan lemah p = 0,034, r = 0,284 antara asupan asam amino taurin dengan aktivitas superoksida dismutase pada pasien osteoartritis lutut. Kesimpulan: asupan asam amino taurin mungkin mempunyai peranan dengan aktivitas superoksida dismutase pada pasien osteoartritis lutut.

The aim of this research was to observe the correlation between taurine amino acid intakes and superoxide dismutase activities on knee osteoarthritis patients. In osteoarthritis there is an imbalance state between pro oxidant and anti oxidant causing oxidative stress. The enzymatic anti oxidant superoxide dismutase plays an important role in stopping the occurrence of oxidative stress by cutting off the free radicals chain reaction from the beginning. Superoxide dismutase works by catalyzing superoxide into hydrogen peroxide. Osteoarthritis cases are known by the increase of superoxide and the decrease of superoxide dismutase activities. Taurine is an amino acid that is found abundant in human body that does not play a role in protein synthesis reaction. Taurine amino acid is found in several food sources including fish, meat, and seafood. Taurine amino acid has several characteristics including anti oxidant, anti inflammatory, and chondro protective. This study used cross sectional design with 56 knee osteoarthritis subjects recruited through consecutive sampling. Taurine intake was obtained by semiquantitative FFQ method. The superoxide dismutase activity sample was obtained from whole blood and measured using RANSOD SD 125 with spectrophotometric method. The statistical test used correlation test with SPSS. The mean age was 50.75 6.17 years old, with 89.3 of them were females. Median for taurine intakes was 59.77 15.96 ndash 278.57 mg per day. Median for the superoxide dismutase activities was 274.97 152.48 ndash 360.97 unit per ml, and 64.3 of the subjects with increasing superoxide dismutase activity. This research found a positive yet low significant correlation p 0,034, r 0,284 between taurine amino acid intakes and superoxide dismutase activity in patients with knee osteoarthritis. Conclusion The taurine amino acid intake may have a role with the superoxide dismutase activity in patients with knee osteoarthritis.
"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
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UI - Tesis Membership  Universitas Indonesia Library
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"Forty-eight male weanling rats (91 g) were utilized to study the nutritional adequacy of cooked polished white rice. Rats
were individually housed, and allowed ad libitum access to one of six treatment diets. Treatment diets were 1) polished
white rice plus 10% casein and 0.18% methionine, CAS, 2) polished white rice, WHR, 3) polished white rice plus
0.45% lysine, LYS, 4) polished white rice plus0.40% methionine, MET, 5) polished white rice plus 0.30% threonine,
THR, 6) polished white rice plus 0.45% lysine, 0.40% methionine, and 0.40% threonine, COM. Rice was cooked prior
diet formulation using a 3 to 1 ratio of water to rice. Vitamins (AIN-76) and AIN minerals were added to all diets to
meet NRC (1978) requirements. Rats fed CAS diets were significantly heavier on d 21 (P<0.05) than rats on COM,
LYS, MET, THR, or WHR diets, (219.9 vs. 171.6, 153.2, 153.2, 148.3, or 155.4 g respectively). Supplementation of the
most deficient essential amino acids, lysine (LYS) or methionine (MET) did not improve (P>0.05) rat performance over
WHR fed rats, Average daily gain (ADG) for CAS was 6.1 g/d and ADG for LYS and MET was 3.0 g/d. The addition
of threonine (THR) significantly (P<0.05) reduced ADG when compared to WHR diets (2.7 vs. 3.0 g/d). When rats
were fed to COM diet significant (P<0.05) improvement in ADG was observed compared to WHR fed rats (4.8 vs. 3.0
g/d). The increased gains achieved with COM diet and the poor gains observed with the single amino acid diets (LYS,
MET, or THR) would suggest that polished white rice is limiting in more than one essential amino acid."
Lembaga Penelitian Universitas Indonesia, 2003
Artikel Jurnal  Universitas Indonesia Library
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Alberto Christopher
"Apoptin merupakan protein dari virus anemia ayam yang dapat menginduksi apoptosis sel kanker. Penggunaannya sebagai senyawa antikanker dapat mengatasi kelemahan metode kemoterapi. Deteksi massa molekular apoptin dilakukan dengan SDS-PAGE memiliki keberagaman hasil dan asam-asam amino rekombinan dalam apoptin disinyalir menyadi penyebabnya. Karakterisasi asam-asam amino rekombinan dalam apoptin untuk membuktikan hipotesis tersebut dilakukan dengan dua variasi rekombinan plasmid, yakni modifikasi 12 histidin dan modifikasi 12 histidin-8 arginin yang ditransformasikan pada Escherichia coli DH5α. Kedua variasi modifikasi plasmid ini mendapatkan perlakuan yang sama. Transformasi plasmid dalam penelitian ini menggunakan metode heat shock dengan suhu 42oC dan bantuan CaCl2 dalam pembentukan sel Escherichia coli DH5α kompeten. Penentuan konsentrasi apoptin dilakukan dengan metode Lowry dan BSA sebagai protein standarnya. Deteksi massa molekular apoptin oleh metode SDS-PAGE dilakukan dengan konsentrasi gel sebesar 15% dan mendeteksi adanya pita protein di bawah 30 KDa. Uji karakterisasi asam amino yang dilakukan dengan Liquid Chromatography Mass Spectroscopy menunjukkan bahwa adanya konsentrasi asam amino berlebih dalam apoptin sehingga meningkatkan deteksi massa molekularnya oleh SDS-PAGE.

Apoptin is a protein from chicken anemia virus which could induce tumor cell apoptotic. The use of apoptin as anticancer could overwhelm chemotheraphy weaknesses. Apoptin molecular weight detection conducted by SDS-PAGE had various results while the recombinant amino acids are the suspects. Recombinant amino acids characterization of apoptin in order to prove the hypothesis was conducted by two variants of recombinant plasmid, which were 12 histidine modification dan 12 histidine-8 arginine modification, transformed into Escherichia coli DH5α. These two variations were given the same treatment. Heat shock method was used in plasmid transformation at 42oC and CaCl2 treatment was used in order to create Escherichia coli DH5α competent cells. Apoptin concentration determination was conducted by Lowry method and BSA was used as protein standard. Molecular weight detection of apoptin by SDS-PAGE was conducted using 15% gel concentration and there was protein band detected below 30 KDa. Amino acids characterization test conducted indicate that there are excess amino acids concentration in apoptin as the cause of increasing molecular weight detection by SDS-PAGE.
"
Depok: Fakultas Teknik Universitas Indonesia, 2014
S55247
UI - Skripsi Membership  Universitas Indonesia Library
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Dupont, Anton
"The statement contained in the motto cited above, made as early as the year 1906, still has lost nothing of its value for the present time. Although innumerable investigations have been carried out on proteins, and much insight was gained from this, a lot of problems connected with the proteins, remain to be solved. As is well known, proteins are found in nature in the animal and vegetable kingdoms. The differences between these two groups of proteins are situated in the number and the amount of the various acids present in these proteins.
Up to comparatively recent years, however, the knowledge of the requirement for the amino acids in the diet was limited to in-formation obtained with the young rat. This animal was able to grow when receiving only nine amino acids: namely histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine; and grew significantly better when arginine was also provided. These ten amino acids were called "essential" for the growing rat, the remaining amino acids were called "non essential". However, there was no implication that the dietetically non-essential amino acids are of little or no value. In spite of the cautions issued, there has been a general tendency to assume, that the above classification has a very broad application. The investigations made by Rose et all. (1,2,3,4,5), have revealed that for adult man only eight amino acids are essential, namely, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine. Moreover in these investigations, it was demonstrated, that only foods with sufficient caloric and nitrogen supply and adequate amounts of these eight amino acids simultaneously are able to main﷓ thin a nitrogen balance. This fact is of far reaching importance, because, from this it follows, that the nutritive value of a meal, with respect to the proteins, is determined by the total amount of essential amino acids - in the proper proportions - present in the constituents of the meal. In Indonesia, rice is the most important constituent of a meal. However rice contains such a small amount of some essential amino acids, that even larger use is not sufficient to meet the requirements of man. Therefore, other foods, which complete this shortage, must be used in combination with rice.
In general, in Indonesia, foods containing animal protein are relatively scarce; therefore, it is necessary to replace these with foods containing vegetable protein. An important source of plant protein is found in the soybean. As a general rule, the soybean yields fair crops, without asking too much care. Botanically, the soybean has been referred to as Glycine hispida (Moench) Max. Recent studies indicate that the correct botanical name should be Glycine max (L.) Merril according to international botanical rules (6).
The early history of the soybean, like that of most important food crops, is lost in obscurity. In the Far East story tellers for centuries have related, tales of the remarkable history of the soybean. Ancient Chinese literature reveals that the soybean was extensively cultivated and highly valued as a food. There it is said to be one of the grains planted by one of the gods of agriculture, named Hou Tsi. The first written record of the plant is contained in the books Pen Tsao Kong Mu, a materia medica written by Emperor Sheng Nung in 2838. B.C.
In many of the early writings advice of agricultural experts is given on soil preferences, proper time of planting, methods and rates of planting, the best varieties to plant under different conditions and for different uses, time to harvest, methods of storage, and utilization of the many varieties for different purposes. Some of this expert advice goes as far back as 2207 B.C. The soybean was included in the second class of drugs, and was regarded as having many medicinal virtues; it was regarded as a specific remedy for the propel functioning of the heart, and other organs."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1954
D114
UI - Disertasi Membership  Universitas Indonesia Library
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Fransisca Putri Tungga Dewi
"ABSTRAK
Secondary iron overload pada thalassemia mayor terjadi karena eritropoiesis inefektif dan tranfusi berkala. Besi melebihi transferin sehingga banyak non transferin bound iron NTBI yang mengkatalisasi terjadinya ion radikal bebas yang merusak jaringan. Pengendapan besi pada saluran cerna mengakibatkan perubahan fungsi, kerusakan organ, gangguan ketersediaan asam amino. Iron overload dikurangi dengan kelasi besi. Transferin merupakan kelator alami tubuh terdiri asam amino dominan alanin, leusin, glisin, asam aspartat. Berdasarkan penelitian, pasien iron overload memiliki transferin lebih rendah dibandingkan non iron overload. Penelitian bertujuan mengetahi perubahan status besi, profil asam amino dan hubungan iron overload dengan profil asam amino. Parameter yang diteliti : besi serum, unsaturated iron binding capacity UIBC , total iron binding capacity TIBC , feritin, saturasi transferin, indeks transferin, alanin, leusin, glisin, asam aspartat. Desain penelitian kohort dengan 21 subjek, yaitu 13 thalassemia beta mayor dan 8 thalassemia beta HbE. Hasil penelitian didapatkan perubahan status besi bermakna yaitu peningkatan feritin pasca transfusi, penurunan feritin pasca kelasi 1 bulan, peningkatan kadar besi pasca kelasi 3 bulan. Perubahan asam amino bermakna yaitu penurunan alanin, leusin, serta peningkatan glisin pasca kelasi 1 bulan Terdapat hubungan kuat, bermakna searah antara indeks transferin dan alanin pre transfusi. Terdapat hubungan kuat, bermakna, searah antara indeks transferin dengan alanin dan glisin pasca transfusi.

ABSTRACT
Secondary iron overload in thalassemia major occurs due to ineffective erythropoiesis and periodic transfusions. The excess of iron exceed transferrin so there are many non transferrin bound iron NTBI that induce tissue damaging free radical ion. Accumulation of iron in intestine can lead to changes in the function, organ damage, lack of amino acid availability. Iron overload can be reduced by iron chelation. Transferrin is the body 39 s natural chelator comprising of dominant amino acid alanine, leucine, glycine, aspartic acid. Research found that transferrin were lower in iron overload patients. This study aims to acquire the changes of iron status, amino acid profile, and correlation between iron overload and amino acid profile. Studied parameter were serum iron, unsaturated iron binding capacity UIBC , total iron binding capacity TIBC , ferritin, transferrin saturation, transferrin index, alanine, leucine, glycine, aspartic acid. The study design were cohort with 21 subjects consisted of 13 beta major thalassemia and 8 beta Hbe thalassemia. The result showed significant iron status changes ferritin increased post transfusion, ferritin decreased after 1 month chelation and serum iron increased after 3 months chelation. Significant amino acid profile changes decreased of alanine and leucine, and glycin increased after 1 month chelation. There rsquo s significant correlation between transferrin index and alanine pre transfusion. There rsquo s significant correlation between transferrin index and alanine, glycine after 3 month chelation. "
2017
T55642
UI - Tesis Membership  Universitas Indonesia Library
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