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"Latar Belakang: Amelogenin merupakan gen yang umum digunakan dalam identifikasi dimorfisme seksual, namun riset dan laporan kasus melaporkan adanya kegagalan dalam amplifikasi dikarenakan delesi pada AMELY. Tujuan: Menganalisis frekuensi delesi AMELY pada populasi pria di Indonesia. Metode: Pemeriksaan DNA dengan amplifikasi multipleks PCR menggunakan gen AMXY 1F/2R dan SRY. Hasil dan Kesimpulan: Satu dari 405 sampel penelitian mengalami delesi pada gen AMELY pada populasi di Indonesia.

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Background: The Amelogenin gene represents the gender marker most widely used for human identification. However, some failures in sex-typing have been observed globally.

Aim: In this study, we could approximate the population frequency of AMELY negative among Indonesian population.

Methods: Multiplex PCR using primers AMXLY 1F/2R and SRY.

Results and Summary: One of 405 sample are indicated as AMELY negative in an Indonesian Population."

"[ABSTRAKTelah dilakukan penelitian mengenai identifikasi spesies dan distribusilarva udang mantis di Teluk Banten selama bulan Oktober 2013--November 2013.Penelitian bertujuan untuk mengukur efektivitas aplikasi DNA barcoding dalamidentifikasi larva udang mantis dan mempelajari pola distribusinya di TelukBanten. Larva udang mantis sebanyak 138 individu dikoleksi denganmenggunakan jaring larva dengan besar mulut 30x30 cm2 dan besar jaring sebesar500 μm dari 6 stasiun penelitian. Daerah COI sebagai penanda DNA barcodingefektif dapat digunakan untuk identifikasi larva udang mantis dengan variasiintraspesies sekuen COI berkisar antara 0,7--2,4%. Distribusi larva udang mantisberpusat di Stasiun 4 yang ditandai dengan tingginya kelimpahan larva udangmantis pada lokasi tersebut (P<0,005; ANOSIM). Ordinasi NMDS danklusterisasi berdasarkan jarak Bray-Curtis menunjukkan distribusi larva udangmantis dipengaruhi oleh kondisi perairanTeluk Banten. Faktor lingkungan yangmemengaruhi kelimpahan larva udang mantis adalah suhu, salinitas dan kecerahandengan nilai R2 adjusted sebesar 94,5% (P<0,05). Distribusi, kelimpahan, dankomposisi larva udnag mantis di Teluk Banten juga dipengaruhi oleh polaperilaku larva (vertical migration) dan arah arus yang memengaruhi perairanTeluk Banten. Distribusi kelimpahan larva pada lokasi penelitian selama bulanOktober--November 2013 bergerak kearah barat Teluk Banten.

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ABSTRACTPlanktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.;Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.;Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater., Planktonic larvae of stomatopoda were collected at six stations in BantenBay from October 2013 to November 2013, aimed at assessing effectiveness ofusing COI gene for barcoding stomatopoda larvae and studying its distribution inBanten Bay. A total of 138 stomatopod larvae were obtained by deploying larvaltrap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10minutes just beneath the surface. Five species of stomatopod successfullyidentified using COI gene as barcode marker. Variation of intraspecies for COIgene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution ofstomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance atsix stations in Banten Bay affected by temperature, salinity, and visibility withscore of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity ofstomatopods larvae are affected by vertical migration and current on Teluk Bantenwater.]"

"ABSTRAKIdentifikasi forensik sangat penting dalam penanganan kasus kriminal, kecelakaan, maupun bencana alam. Identifikasi forensik dilakukan untuk membantu proses investigasi dan mengembalikan korban kepada keluarga yang benar. Salah satu metode yang dapat dilakukan untuk membantu proses identifikasi forensik ialah analisis DNA dari sampel tulang. Tulang dipilih karena merupakan bagian tubuh yang paling awet dari proses pembusukan dan pelapukan dibandingkan bagian tubuh lainnya. Namun, tulang yang ditemukan di tempat kejadian perkara memiliki jumlah yang terbatas, sehingga penggunaan tulang untuk ekstraksi DNA harus seminimal mungkin. Tujuan dari penelitian adalah untuk mendapatkan berat sampel yang efisien sehingga tidak merusak bentuk tulang sebagai barang bukti, namun dapat menghasilkan kesimpulan yang kuat. Penelitian dilakukan dengan mengektraksi DNA dari sampel tulang femur yang telah dijadikan bubuk terlebih dahulu. Bubuk sampel tulang diekstraksi menggunakan metode ektraksi organik fenol-kloroform. Hasil ekstraksi DNA kemudian dikuantifikasi menggunakan Real-Time PCR (RT-PCR). Hasil kuantifikasi DNA sampel tulang dari berat 100 mg, 150 mg, 200 mg, 250 mg, dan 300 mg didapatkan persamaan regresi linear y = 0,032x + 0,417, dengan y = berat DNA dan x = berat sampel tulang. Berdasarkan perhitungan dari rumus persamaan tersebut, berat minimal sampel tulang adalah sebesar 18,22 mg untuk 1 ng DNA.

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ABSTRACTForensic identification is very important step for handling criminal, accident, and natural disaster cases. Forensic identification was done to assist the process of investigation and return the victim’s body back to their family. One of many methods that can help forensic identification process is DNA analysis from bone sample. Bone chosen because it’s weathering and decaying is slower than other tissues. Bones, found at the crime scene in limited amount, should be used with precaution. The aims of this research is to determine the minimum quantity of bone sample without damaging the bone profile as an evidence. Research was carried out by extracting DNA from femur bone samples that had been powdered beforehand. Bone powder samples were extracted using organic phenol-chloroform extraction. Quantification of DNA was performed by using Real-Time PCR (RT-PCR). The result of DNA quantification from bone samples quantity of 100 mg, 150 mg, 200 mg, 250 mg, and 300 mg were plotted to obtain linear regression equation y = 0,032x + 0,417, with y = DNA quantity and x = bone sample quantity. Derived from the equation, the minimum quantity of bone sample is 18,22 mg for 1 ng DNA. "

"This book, written by leading experts, summarizes our current knowledge about plastid and mitochondrial genomes in all major groups of algae and land plants. It also includes chapters on endosymbioses, plastid and mitochondrial mutants, gene expression profiling and methods for organelle transformation. "

"ABSTRAKHuman papillomavirus HPV adalah virus DNA yang dapat menginfeksi bagian basal sel epitel leher rahim wanita melalui luka sehingga meningkatkan kasus kanker serviks di Indonesia. Penelitian mengenai pengembangan obat terhadap penyakit kanker serviks berbasis vaksin DNA terapeutik telah dilakukan melalui konstruksi plasmid rekombinan antigen E7 HPV-16 pada sistem ekspresi mamalia. Vektor plasmid pcDNA 3.1 5.428 pb yang digunakan pada penelitian berhasil dikonstruksi melalui proses digesti pada situs NheI dan ligasi dengan fragmen acak gen E7 294 pb sehingga membentuk plasmid rekombinan pcDNA-E7 CADB. Plasmid rekombinan hasil ligasi diklona ke dalam sel E. coli TOP 10 melalui proses transformasi heat shock. Analisis hasil penelitian menunjukkan bahwa 5 koloni mengandung plasmid rekombinan pcDNA-E7 CADB. Analisis orientasi arah gen melalui PCR dan digesti pada 5 koloni menghasilkan 2 koloni plasmid positif dengan arah orientasi 5 rsquo; ke 3 rsquo; pada koloni nomor 5 dan 7. Kedua koloni menunjukkan bahwa fragmen gen E7 CADB berhasil disisipkan pada vektor pcDNA 3.1 dan berhasil diklona ke dalam E. coli TOP 10.

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ABSTRACTHuman papillomavirus HPV is a DNA virus that can infects the basal cells of the female cervix through wounds in which it may increase the risk of cervical cancer in Indonesia. There has been a drug development research to treat cervical cancer based on therapeutic DNA vaccine via constructing recombinant plasmid of HPV 16 E7 in mammalian expression system. pcDNA 3.1 plasmid vectors 5.428 bp which were used in this research are successfully construced through the digestion process at NheI site and the ligation process with shuffling fragments of E7 gene 294 bp which created pcDNA E7 CADB recombinant plasmid. Recombinant plasmid which is the result of the ligation process is cloned into TOP 10 Escherichia coli cell through a transformation process called heat shock. The result of this research displays 5 colonies containing pcDNA E7 CADB recombinant plasmid. Analysis of gene direction orientation through PCR and digestion of 5 colonies displays positive plasmid on 2 colonies with 5 rsquo ndash 3 rsquo direction on colony unit 5 and colony unit 7. Result of the 2 colony shows that E7 CADB gene fragment successfully inserted into the NheI site of pcDNA 3.1. It also resulted in cloning completion of E7 CADB gene fragments into TOP 10 E. coli."

"ABSTRAKPerkembangan Ilmu Pengetahuan telah melahirkan banyak instrumen-instrumen yang membantu kehidupan manusia diantaranya penelitian tentang genetika. Diketahuinya Deoxyribonucleic acid DNA sebagai pembawa unsur kimia untuk informasi genetik merupakan gen yang meneruskan informasi biologis dari induk kepada keturunannya dan telah membantu kita untuk mengetahui file-file khas karakter tubuh. Penggunaan resume hasil tes DNA dalam penyelesaian sengketa di Pengadilan Negeri bukanlah hal baru. Dalam beberapa tahun terakhir, bukti tes DNA telah menjadi alat bukti yang penting bagi pemecahan kasus di Pengadilan Negeri khususnya terkait hubungan darah. Penerapan alat bukti ilmu pengetahuan dan teknologi termasuk resume hasil tes DNA dalam penyelesaian sengketa di pengadilan telah menjadi instrumen penting dalam mengungkapkan kebenaran formil. Namun, sangat disayangkan eksistensi dari hasil tes DNA itu sendiri belum memiliki pengaturan perundang-undangan tersendiri dalam pembuktian di hukum acara perdata di Indonesia. Berdasarkan latar belakang tersebut, Penulis melakukan penelitian yang bertujuan untuk mengetahui bagaimana peran, fungsi serta prosedur pengajuan hasil tes DNA sebagai alat bukti dalam penyelesaian perkara perdata di Pengadilan Negeri termasuk bagaimana hakim mempertimbangkan dalam putusan. Penelitian ini menggunakan metode yuridis normatif dengan menggunakan metode penelitian kepustakaan dan dipadu dengan wawancara narasumber. Dari penelitian yang dilakukan didapatkan hasil bahwa hasil tes DNA yang berupa resume sudah membantu hakim dalam membuat pertimbangan atas putusan dan atau penetapan yang dijatuhkan dan dapat dikategorikan sebagai alat bukti surat yang tergolong akta otentik dan dapat pula diperkuat dengan keterangan ahli sebagai alat bukti dalam proses pembuktian perkara perdata.

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ABSTRACTThe development of science has spawned many instruments that helps human life, such as research on genetics. Deoxyribonucleic acid DNA as the carrier of the chemical element for genetic information is a gene that passes biological information from the mother to her children and has helped us to know the typical character files of the body. The use of resumes of DNA test results in dispute resolved at the District Court is not new. The application of scientific and technological evidence including resumes of DNA test results in dispute resolvement in court has become an important instrument in revealing formal trusth. However, it is unfortunate that the existence of the DNA test does not yet have own legislation in the provision of civil procedure law in Indonesia. Based on the background, the author conducted a study that aims to find out how the roles, the functions and the procedure of filing DNA test results as evidence in the settlement of civil cases in the District Court including how judges consider in decisions. This research uses normative juridical method using literature research method and combined with interviews of resource persons. From the research results of DNA testing in the form of resumes have helped judges in making consideration of the verdict and establishment and also can be categorized as a letter proof evidence that belong to authentic deed and can also be reinforced with expert information as evidence in the process of proving civil cases. "

"ABSTRAKKami mempelajari sifat transport muatan menggunakan molekul DNA aperiodik dan molekul DNA G4. Molekul DNA aperiodik mempelajari pengaruh nilai loncatan inter-strand pada karakteristik I-V. Molekul DNA G4 mempelajari pengaruh medan listrik dan medan magnet pada panjang lokalisasi, density of state DOS , dan karakteristik I-V. Sifat transport muatan dipelajari dengan menghitung probabilitas transmisi, karakteristik I-V, dan memberikan pengaruh medan listrik dan medan magnet. Pada molekul DNA aperiodik, dipelajari pengaruh nilai hopping inter-strand terhadap probabilitas transmisi dan karakteristik I-V. Probabilitas transmisi dihitung dengan metode transfer matriks, sedangkan karakteristik I-V dihitung dengan formula Launder-Buttiker. Pada molekul DNA G4, dipelajari pengaruh medan listrik dan medan magnet terhadap panjang lokalisasi, density of state DOS, dan karakteristik I-V. Panjang lokalisasi dihitung menggunakan metode transfer matriks, dan density of state DOS serta karakteristik I-V dihitung dengan metode fungsi Green.

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ABSTRACTThe charge transport of aperiodic DNA and G4 DNA molecule have been studied. On the aperiodic DNA molecule, the effect of the inter-strand hopping value on the I-V characteristic has been studied. The effect of the electric and magnetic field on the localization length, density of state (DOS), and I-V characteristics were studied G4 DNA molecule. Charge transport was studied by calculating the transmission probability, the I-V characteristic, by considering the effect of the electric and the magnetic field. In the aperiodic DNA molecule, we study the effect of hopping inter-strand on transmission probability and I-V characteristics. The transmission probability was calculated by the matrix transfer method, while the I-V characteristic was calculated by the Launder-Buttiker formula. In G4 DNA molecule, we studied the effect of the electric and magnetic field on the localization length, density of state (DOS), and I-V characteristic. Localization length was calculated using matrix transfer method, and density of state (DOS) and I-V characteristic was calculated by Green function method."

"DNA polimerase merupakan sebuah enzim yang memiliki aplikasi luas dalam dunia bioteknologi. Peran DNA polimerase dalam sintesis DNA membuat DNA polimerase sebuah reagen yang sering digunakan pada berbagai metode amplifikasi DNA. Proses sintesis DNA pada metode-metode tersebut menggunakan suhu tinggi, sehingga dibutuhkan DNA polimerase yang tidak terdenaturasi pada suhu tinggi atau dengan kata lain termostabil. Salah satu sumber DNA polimerase termostabil adalah bakteri termofilik Geobacillus thermoleovorans Strain SGAir0734 (GBK Pol) yang diisolasi dari mata air panas Batu Kuwung, Banten, Indonesia. Pada penelitian sebelumnya, telah diuji coba produksi dari GBK Pol dengan suhu purifikasi pada rentang 60 – 80℃ dimana diduga terjadi denaturasi dari enzim. Maka dari itu, pada penelitian ini dilakukan purifikasi GBK Pol sekuens utuh dan parsial dengan metode immobilized metal affinity chromatography (IMAC) dengan rentang suhu 40 – 60℃ dan uji aktivitas amplifikasi DNA dengan metode loop mediated isothermal amplification (LAMP) dengan rentang suhu 25 – 60℃. Hasil uji Lowry menunjukkan bahwa pemanasan 60℃ menghasilkan GBK pol dengan konsentrasi tertinggi, yaitu sekitar 134 µg/ml untuk plasmid sekuens utuh. GBK pol hasil purifikasi kemudian diuji aktivitasnya dengan LAMP, dimana reaksi pada suhu 40℃ selama 2 jam menghasilkan pita yang jelas pada elektroforesis dan menghasilkan konsentrasi DNA tertinggi. GBK pol dari hasil purifikasi dengan pemanasan 60℃ menghasilkan konsentrasi DNA tertinggi pada LAMP suhu 40℃ baik untuk sekuens parsial dan utuh.

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DNA polymerase is an enzyme that has a wide application in the world of biotechnology. DNA Polymerase’s role in synthesizing DNA makes DNA polymerase a favorable reagent in various DNA amplification methods. The synthesis process in these methods is done in high temperatures, thus a DNA polymerase that won’t experience denaturation in high temperatures, or in other words thermostable, is needed. One source of thermostable DNA polymerase is the thermophilic bacteria Geobacillus thermoleovorans Strain SGAir0734 (dubbed GBK Pol) that has been isolated from Batu Kuwung hot springs, Banten, Indonesia. In the previous study, a production trial of GBK pol has been carried out with purification temperatures that range between 60 – 80℃, where denaturation of the enzyme is suspected to have happened. As such, in this study, lower temperatures of 40 – 60℃ are employed in the purification of GBK Pol with immobilized metal affinity chromatography (IMAC) as well as in the activity study with loop mediated isothermal amplification (LAMP) with reaction temperatures of 25 – 60℃. The Lowry assay results show that the highest GBK pol concentration is achieved with 60℃ heat treatment, with a concentration of around 134 µg/ml for full sequence plasmid. Purified GBK pol are then tested with LAMP, where isothermal amplification at 40℃ for 2 hours resulted in the clearest bands during gel electrophoresis as well as highest concentrations of DNA. The highest concentration of DNA is achieved from GBK pol with 60℃ heat treatment, suggesting that 60℃ is the optimal temperature for purification."

"Berdasarkan data WHO pada pertengahan Juli 2021 lebih dari 185,2 juta orang di seluruh dunia terinfeksi virus corona atau Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Virus ini menyerang penapasan manusia yang dapat mengakibatkan infeksi paru-paru pada manusia dan bahkan dapat menyebabkan kematian. Tercatat bahwa lebih dari 4 juta orang di seluruh dunia meninggal akibat terinfeksi virus corona. Di Indonesia sendiri pada pertengahan Juli 2021 tercatat lebih dari 2,4 juta orang ternfeksi virus corona dan lebih dari 65,4 ribu orang meninggal akibat terinfeksi virus corona. Berdasarkan data tersebut, perlu dilakukan analisis kekerabatan virus SARS-CoV-2 untuk mengurangi penyebaran dan memberikan batasan sosial dari negara satu dengan negara lainnya. Identifikasi kekerabatan dari virus covid-19 dan penyebarannya dapat dilakukan dengan cara pembentukan pohon filogenetik dan clustering. Pada penelitian ini pohon filogenetik akan dibangun berdasarkan metode Hierarchical Clustering dengan menggunakan metode Multiple Encoding Vector dan K-Mer berdasarkan translasi DNA kodon menjadi asam amino. Jarak Euclidean akan digunakan untuk menentukan matriks jarak. Penelitian ini selanjutnya menggunakan metode K- Means Clustering untuk melihat penyebarannya, dimana nilai k ditentukan dari jumlah centroid yang dihasilkan dari metode Hierarchical Clustering. Penelitian ini mengambil sampel barisan DNA SARS-CoV-2 dari beberapa negara yang tertular. Dari hasil simulasi, nenek moyang SARS-CoV-2 berasal dari China. Hasil analisis juga menunjukkan bahwa leluhur covid-19 yang paling dekat dengan Indonesia berasal dari India, Australia dan Spanyol. Selain itu dari hasil simulasi dihasilkan bahwa barisan DNA SARS-CoV-2 terdiri dari 9 cluster dan cluster keenam adalah kelompok yang memiliki anggota paling banyak. Hasil analisis juga menunjukkan bahwa metode ini sangat opitimal dalam pengelompokan data dengan nilai 97.4%.

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Based on WHO data in middle of July 2021, Coronavirus or Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is infecting more than 185.2 million people worldwide. The virus attacks human breathing, which can cause lung infections and can even cause death. More than 4 million people worldwide have died due to being infected with the coronavirus. In Indonesia alone, in mid-July 2021, there were more than 2.4 million people infected with the corona virus and more than 65.4 thousand people died from being infected with the corona virus. Based on those covid-19 survivor data, it is necessary to carry out a kinship analysis of the coronavirus to reduce its spreading. Identification of the kinship of the covid- 19 virus and its spread can be done by forming a phylogenetic tree and clustering. This study uses the Multiple Encoding Vector method and K-mer based on translation DNA codon to amino acid in analyzing sequences and Euclidean Distance to determine the distance matrix. This research will then use the Hierarchical Clustering method to determine the number of initial centroids and cluster, which will be used later by the K-Means Clustering method kinship in SARS-CoV-2 DNA sequence. This study took samples of DNA sequences of SARS-CoV-2 from several infected countries. From the simulation results, the ancestors of SARS-CoV-2 came from China. The results of the analysis also show that the closest ancestors of covid-19 to Indonesia came from India, Australia and Spain. In addition, the ancestors of SARS-CoV-2 came from China. The SARS- CoV-2 DNA sequence is also consisted of 9 clusters, and the sixth cluster is the group that has the most members. The results also show that this method is very optimal in a grouping of data with a value of 97.4%."

"Metode Taqman MGB real time PCR yang cepat merupakan kunci pengawasan pemalsuan daging yang efektif. Penelitian bertujuan mengevaluasi kuantitas, kualitas DNA produk olahan daging babi, serta kandungan DNA babi produk olahan daging sapi yang diduga mengandung babi menggunakan Taqman MGB real time PCR untuk memverifikasi label. Lima produk olahan daging babi, 30 produk olahan daging sapi: dendeng, abon, baso, dan daging asap sebagai sampel, serta daging babi segar sebagai kontrol positif diekstraksi, diukur konsentrasi, kemurnian DNA, dielektroforesis serta diamplifikasi dengan realtime PCR. Konsentrasi, kemurnian DNA, nilai Ct sampel diuji ANAVA satu arah dilanjutkan uji Tukey, kecuali nilai Ct produk olahan daging sapi. Integritas DNA genomnya dianalisis deskriptif. Hasil uji ANAVA menunjukkan ada pengaruh nyata (P˂0,05) konsentrasi, kemurnian DNA dan nilai Ct. Hasil uji Tukey produk olahan daging babi: ada beda nyata konsentrasi DNA sampel dan kontrol positif (P˂0,05), kecuali kornet (P˃0,05). Kemurnian DNA baso dan daging asap berbeda nyata (P˂0,05) dengan kontrol positif. Nilai Ct sampel dan kontrol positif berbeda nyata (P˂0,05), kecuali dendeng (P˃0,05). Hasil uji Tukey produk olahan daging sapi: konsentrasi DNA baso dan daging asap berbeda nyata (P<0,05) dengan kontrol positif, kemurnian DNA kornet berbeda nyata (P<0,05) dengan kontrol positif. Semua DNA genom sampel terfragmentasi ukuran terendahnya sekitar 250 bp dimiliki kornet dan abon. Produk olahan daging dapat meningkat kuantitas DNAnya dan menurun kualitas DNAnya tergantung pada suhu dan bahan tambahan yang diberikan. Tiga puluh produk olahan daging sapi tidak mengandung DNA babi menggunakan Taqman real time PCR yang sensitif dan cepat serta terverifikasi mematuhi peraturan label.

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The fast Taqman MGB qPCR method is key to effective meat adulteration surveillance. This research aimed to evaluate the quantity, quality of DNA from processed pork products and the content of pork DNA in processed beef products suspected of containing pork DNA using the Taqman MGB qPCR to verify labels. Five processed pork products, 30 processed beef products: corned, jerky, shredded, meatballs, and smoked meat were used as samples as well as and fresh pork as a positive control were extracted, DNA concentration and purity were measured, electrophoresed, and amplified with qPCR. The DNA concentration, purity, and Ct value were tested by one-way ANOVA followed by the Tukey test, except for the Ct value of processed beef products. The genomic DNA integrity was analyzed descriptively. The ANOVA showed a significant effect (P˂0.05) on the concentration and purity of DNA and Ct value. Tukey test results for processed pork products: there was a significant difference (P˂0.05) in the DNA concentration of the samples and positive controls, except for corned (P˃0.05). The DNA purity of pork meatballs and smoked pork was significantly different (P˂0.05) from the positive control. The Ct values of the samples and positive control were significantly different (P˂0.05), except for jerky (P˃0.05). The results of the Tukey test for processed beef products: the DNA concentration of beef meatballs and smoked beef was significantly different (P<0.05) with the positive control, and the DNA purity of corned beef was significantly different (P<0,05) with positive control. All genomic DNA samples were fragmented with the smallest size of about 250 bp experienced by corned and shredded. Processed meat products can increase the quantity of DNA and decrease the quality depending on temperature and additives. Thirty processed beef products did not contain pork DNA using the sensitive and fast Taqman qPCR and verified to comply with label regulations."