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"Pit Fissure Sealant berbahan resin merupakan salah satu produk pencegahan karies. Pada penelitian sebelumnya, ditunjukkan adanya pelepasan komponen dan material tersebut ke lingkungannya yang menimbulkan respon hipersensitifitas. Tujuan : untuk mengetahui biokompatibilitas dari Resin Pit Fissure Sealant terhadap sel keratinosit kulit yang dicerminkan dari viabilitas sel HaCaT. Material dan Metode: Spesimen Resin Pit & Fissure Sealant dibuat pada cetakan akrilik (N=18; diameter 15mm; ketebalan 1mm) menurut ISO 4049 dan dipolimerisasi dengan UV dari QTH (Quartz Tungsten Halogen) ( = 400 nm). Spesimen dipersiapkan dan disterilisasi untuk menghindari kontaminasi dari bakteri atau jamur. Setelah itu, spesimen direndam dalam DMEM (5mL) dan disimpan dalam inkubator (370C) selama 1, 2, dan 7 hari. Kultur sel dipersiapkan pada 96 well dan diinkubasi selama 24 jam. Rendaman spesimen dipaparkan ke setiap well dan diuji tingkat viabilitas selnya menggunakan MTT assay. Tingkat viabilitas sel diukur dengan microplate reader = 490 nm. Signifikansi diukur dengan metode analisis ragam satu arah Anova. Hasil : Viabilitas sel menurun pada hari pertama dan setelah hari kedua. Kesimpulan : Waktu perendaman mempengaruhi viabilitas sel, tetapi masih cukup aman untuk digunakan untuk perawatan gigi.

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Resin based Pit Fissure Sealant is one of dental caries prevention product. Previous research of resin showed that some components leached into aqueous environment and cause hypersensitivity responses.

Objectives: To observe the biocompatibility of Resin Pit Fissure Sealant due to skin keratinocytes which is determined by viability of HaCaT Cell lines.

Material & Methods : Resin based Pit Fissure Sealant were made in acrylic mould (N= 18; diameter 15mm; thickness 1mm) according to ISO 4049 and polymerized by UV light from QTH (Quartz Tungsten Halogen) ( = 400 nm). Specimen were prepared and sterilized to avoid contamination from bacterial or germs. After that, Specimens were immersed in DMEM (5mL) and stored in incubator (370C) for 1, 2, and 7 days. Cell Culture were prepared into 96 well and stored in incubator for 24 hours. The elution of specimens was exposed into every well, and examined the viability of cells by MTT assay. Viability Cell were counted in 490 nm microplate reader. Significance were measured by One Way Anova.

Results : The viability of HaCaT Cell Lines were decreased in first and after second days.

Conclusion : The elution time of Resin based Pit Fissure Sealant affect the viability of HaCat Cell line, but still safe to be used in dental clinic."

"ABSTRAK Latar Belakang: Impaksi Molar 3 rahang bawah telah diketahui akan meningkatkan resiko fraktur tulang mandibula terutama di daerah angulus mandibula. Fraktur angulus mandibula sering terjadi akibat kecelakaan lalu lintas di Indonesia. Masyarakat belum mengetahui pentingnya odontektomi sebagai langkah awal pencegahan fraktur angulus mandibula.Tujuan: Untuk mengetahui apakah terdapat hubungan dari adanya fraktur angulus mandibula dengan adanya impaksi molar 3 rahang bawah.Material dan Metode: Rekam medis pasien poli Bedah Mulut dan Maksilofasial Rumah Sakit Umum kabupaten Tangerang selama periode Januari 2013-Desember 2017 dikumpulkan dan didapatkan 41 orang dengan fraktur angulus mandibula. Setiap sampel diidentifikasi adanya fraktur angulus mandibula, adanya impaksi molar 3 rahang bawah, posisi erupsi impaksi molar 3 dan kelas impaksi menurut Pell dan Gregory. Data diolah dengan uji Chi Square dan Kolmogorov Smirnov, serta ditentukan Odd Ratio. Uji hipotesis korelatif dilakukan dengan Uji Contingency Coeficient, Phi ? ? ?, Cramer rsquo;s V, dan Kendall rsquo;s Tau-b.Kesimpulan: Ditemukan hubungan antara terjadinya fraktur angulus mandibula dengan adanya impaksi molar 3 bawah mandibula dengan p = 0,01 p < 0,05 dengan Odd Ratio = 4,615; memiliki hubungan korelatif dengan p = 0,010 p < 0,05 dengan kekuatan r = 0,272 lemah . Tidak ditemukan hubungan bermakna antara fraktur angulus mandibula dengan posisi erupsi Suprabony,Infrabony p=0,375 p>0,05 . Tidak ditemukan hubungan bermakna antara fraktur angulus mandibula dengan kelas impaksi menurut Pell dan Gregory p=0,087, p>0,05 .Tidak ditemukan hubungan bermakna antara fraktur angulus mandibula dengan Jenis Kelamin p=0,763 p>0,05 . Tidak ditemukan hubungan bermakna antara fraktur angulus mandibula dengan Usia p=1,000 p>0,05. ABSTRACT Background: Impacted third molar of mandibula have been studied to have a role in increasing mandible fracture especially in the mandibular angle region. Mandibular angle fractures are often the result of traffic accidents in Indonesia. People do not yet know the importance of odontectomy as a first step to prevent fracture of the mandibular angle.Objective: To determine whether there is association or correlation of the presence of angular fracture in the presence of lower third molar impaction.Materials and Methods: Medical records of patients with Oral and Maxillofacial Surgery of Tangerang District General Hospital during the period of January 2013-December 2017 were collected and obtained 41 people with mandibular angle fractures. Each sample identified an mandibular angle fracture, a lower third molar impaction, third molar impaction eruption position and an impaction class according to Pell and Gregory. The data were processed by Chi Square and Kolmogorov Smirnov, and Odd Ratio was determined. Test the correlative hypothesis with Contingency Coefficient, Phy ? ? ?, Cramer rsquo;s V, and Kendall Tau B test.Conclusion: There was found a association between the presence of mandibular angle fracture in the presence of mandibula lower 3 molar impaction with p = 0,01 p 0,05 . There was no significant association between mandibular angle fracture and Gender p = 0,763 p> 0,05 . There was no significant association between mandibular angle fracture and Age p = 1,000 p> 0,05"