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"[ABSTRAKSpirullina sp. telah banyak diteliti karena terkenal akan kandungan yang lengkap serta mampu memenuhi kebutuhan manusia sehari-hari. Kandungan yang dihasilkan akan sangat dipengaruhi oleh keberadaan nutrisi didalam medium tempat mikroalga tumbuh. Penelitian ini akan memanfaatkan beberapa jenis medium yang berbeda untuk selanjutnya dapat melihat bagaimana pengaruhnya terhadap laju pertumbuhan serta kandungan esensial. Beberapa jenis kandungan yang akan diuji diantaranya yaitu protein, klorofil, karbohidrat, serta lipid (lemak). Uji protein dilakukan dengan metode lowry, lipid dilakukan dengan metode bligh dryer dan klorofil dilakukan dengan cara melarutkan. Spirullina sp. ini akan ditumbuhkan di dalam reaktor plat datar dengan masing-masing medium berbeda. Medium yang digunakan adalah medium Walne, medium Zarrouck’s, medium ekstrak daging serta medium ekstrak tauge. Pada penelitian ini, Spirulina platensis dengan kandungan lipid (1,787%) serta klorofil (0,052%) tertinggi diperoleh dari kultivasi dengan Medium Walne. Kandungan protein tertinggi (29,957%) diperoleh dari kultivasi dengan medium Zarrouck dan jumlah biomassa tertinggi diperoleh dari kultivasi dengan Medium Tauge 4%. Melalui penelitian ini, dapat disimpulkan bahwa medium alternatif berupa ekstrak daging dan ekstrak tauge dapat digunakan sebagai pengganti medium komersial, meskipun kandungan protein, lipid serta klorofil tertinggi didapati pada medium komersial.

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ABSTRACTSpirulina sp. has been researched because of its prosperous essential content and its ability to meet people needs everyday. This essential content would be influenced by nutrition contained in medium where microalgae lives. Various mediums will be utilized in this research in order to know the effect of nutrition contained to growth rate and essential content. The essential content that will be tested is lipid, protein, and chlorophyll. Protein test performed by lowry method, lipid test performed by bligh-dryer method, and chlorophyll test performed by acetone solvent. Spirulina platensis will be cultivated in flat plate reactor with 8 variant mediums, namely Zarrouck, Walne, Meat Extract (2%, 4%, 10%) and Bean Extract (2%, 4%, 10%) medium. Highest lipid (1,787%) and chlorophyll (0,052%) accumulation found in Spirulina platensis cultivated in Walne Medium,highest protein accumulation (29,957%) found in microalgae cultivated in Zarrouck Medium, also highest biomass accumulation found in microalgae cultivated in 4% Bean Extract Medium. Through this research, it can be concluded that alternative medium such as Meat and Bean Extract Medium can be used as a substitution of commercial medium, though the highest essential content still found in commercial medium., Spirulina sp. has been researched because of its prosperous essential content andits ability to meet people needs everyday. This essential content would beinfluenced by nutrition contained in medium where microalgae lives. Variousmediums will be utilized in this research in order to know the effect of nutritioncontained to growth rate and essential content. The essential content that will betested is lipid, protein, and chlorophyll. Protein test performed by lowry method,lipid test performed by bligh-dryer method, and chlorophyll test performed byacetone solvent. Spirulina platensis will be cultivated in flat plate reactor with 8variant mediums, namely Zarrouck, Walne, Meat Extract (2%, 4%, 10%) andBean Extract (2%, 4%, 10%) medium. Highest lipid (1,787%) and chlorophyll(0,052%) accumulation found in Spirulina platensis cultivated in Walne Medium,highest protein accumulation (29,957%) found in microalgae cultivated inZarrouck Medium, also highest biomass accumulation found in microalgaecultivated in 4% Bean Extract Medium. Through this research, it can beconcluded that alternative medium such as Meat and Bean Extract Medium can beused as a substitution of commercial medium, though the highest essential contentstill found in commercial medium.]"

"ABSTRAKKebutuhan manusia akan energi fossil semakin lama semakin meningkat. Salahsatu cara yang dapat membantu mengatasi hal ini adalah dengan menggunakanpemanfaatan diesel hijau. Diesel hijau bisa didapatkan dari minyak tumbuhan.Selain itut, bisa didapatkan pula dari mikroalga yaitu Chlorella vulgaris.Mikroalga ini dikenal dengan kandungan lipidnya yang tinggi, denganmempertimbangkan beberapa parameter pendukungnya diantara lain yaituintensitas cahaya, medium, pH serta salinitas. Kandungan lipid tersebut akandiekstrak dengan menggunakan metode Bligh-Dryer yang kemudian direaksikandengan methanol. Masalah berikutnya muncul dari katalis homogen yangbiasanya digunakan namun susah untuk diseparasi, sehingga biodiesel harusdicuci kembali untuk mengembalikan pH serta akan mengurangi kualitasnya.Untuk mengatasi masalah tersebut, katalis heterogen berupa zeolite memilikikemampuan untuk mengkatalisis reaksi tersebut. Kelebihannya yaitu mudah untukdipisahkan serta tidak mengurangi kualitas biodiesel yang dihasilkan. Katalisheterogen memiliki peranan yang sama dengan katalis homogeny. Untukmendapatkan katalis yang optimal, maka digunakan beberapa variasi. Diantaranyayaitu variasi pada saat impregnasi, serta variasi saat reaksi transesterifikasiberlangsung. Diharapkan, dari beberapa variasi ini didapatkan karakterisasi katalisheterogen CuO/Zeolit serta kondisi reaksi trasnesterifikasi yang menghasilkanyield tertinggi. Katalis terbaik didapatkan saat konsentrasi Cu(NO3)2 0,002M sertawaktu perendaman 6 jam, yaitu dengan terimpregnasinya CuO sebesar 1770 ppmdidalam zeolite. Selain itu, variasi reaksi transesterifikasi terbaik didapatkan saatmenggunakan katalis 5%, suhu reaksi 60oC serta waktu reaksi 4jam. Masingmasingmemberikan yield terhadap berat kering sebanyak 4,92%, 6,41% ,4,55%serta konversi sebesar 26,03%, 36,79% dan 28,66%.

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ABSTRACTHuman needs for fossil energy increase every year. Green diesel is the main wayto resolve this world problem. Green diesel produces from vegetable oil. But then,the alternative way came from the uses of microalgae in Chlorella vulgaris typecauses by its simplicity of growing. In the other hand, this microalgae known forits high lipid content by considering several parameter such as light intensity,medium nutrition, pH and also salinity. Lipid content will be extracted by usingBligh-Dryer method which will be reacted with methanol along transesterificationreaction. Beside, there come another matter which is the utilization of homogenycatalyst. The difficulty of separation is the main matter so then biodiesel need tobe washed in case normalizing the pH and this process will decrease the quality ofbiodiesel. To resolve this problem, we?ll be using a heterogen catalyst, zeolite,with ability to catalyst the process. Zeolite is easier to separate from the biodieselso there will not be needed washing process. Heterogent catalyst work as well ashomogeny catalyst. Variation needed to optimize the utilization of heterogencatalyst, which occurred in impregnation and transesterification process. Uponthis variation, highest yield of biodiesel with CuO/Zeolit heterogent catalyst iswhat this research aim for. Best catalyst obtained from Cu(NO3)2 0,002M and 6hours of impregnation. It result a CuO of 1770ppm inside zeolite. Meanwhile,transesterification variation reach the greatest result at 5% of catalyst, 60oC and 4hours of reaction. It lead to a yield from dry weight of 4,92%, 6,41% ,4,55% and aconversion of 26,03%, 36,79% and 28,66% in consecutive."