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"TUJUAN: Tujuan dari penelitian ini adalah untuk menentukan kadar tromboksan B2 pada kultur jaringan plasenta penderita preeklampsia dengan plasenta wanita hamil normal sebagai pembanding.RANCANGAN PENELITIAN: Penelitian ini merupakan studi cross-sectional. Kultur jaringan plasenta penderita preeklampsia (n=13) dan plasenta wanita hamil normal (n=12) dengan usia dan umur kehamilan tidak berbeda bermakna secara statistic. Kultur plasenta menggunakan medium M199 dari sigma dengan 20 % serum menggunakan metode tabung menurut Rand dan dikultur selama 72 jam. Kadar tromboksan B2 diukur dengan spektrofotometri pada panjang gelombang 405 nm. Sebagai petanda bahwa plasenta yang dikultur masih memilik viabilitas set yang baik diukur melalui pemeriksaan human chorionic gonadotropin (hCG).HASIL: Kedua sel baik dari jaringan plasenta penderita preeklampsia maupun dari jaringan plasenta hamil normal memiliki viabilitas sel yang baik. Kadar tromboksan B2 yang terlarut dalam supematan kultur jaringan plasenta penderita preklampsia (887.88± 26.07 pglml) lebih tinggi secara bermakna (P<0.05) dibanding wanita hamil normal (849.82± 24.61 pglml)KESIMPULAN: Kadar Tromboksan B2 pada penderita preeklampsia lebih tinggi dibandingkan pada wanita hamil normal, peningkatan ini bertanggung jawab terhadap terjadinya vasokonstriksi pembuluh darah pada plasenta dan maternal.

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Enhanced Tromboxane B2 (TXB2) Production In Placental Culture In PreeclampsiaOBJECTIVE: To determine tromboxane B2 production in placental culture in preeclampsiaSTUDY DESIGN: The study was a crosssectional study. Placentas were obtained from having woman with normal (n=12) and woman with preeclampsia (n=13) with the same age and gestational age. Placenta tissues were incubated in M199 sigma medium with 20 % serum for 72 hour using the with tube method from Rand. Samples were analyzed spectrophotometrically and with absorbtion at 405 nm for tromboxane B2. hCG was also determined as a marker for cell viability.RESULT : The placentas of women will preeclampsia and from normal pregnanly were viable. The concentration of tromboxane B2 from placental of preeclampsia cultured for 72 hour (887.88±26.07 pg/ml) was significantly higher (p<0.05) than from placental of normal pregnanly (849.83±24.60 pg/ml).CONCLUSION : The concentration of tromboxane B2 from cultures of placental preeclampsia was significantly higher than from cultures of placental of normal pregnanly. The increased tromboxane B2 production in placental culture could be responsible for increased placental and maternal blood vessel vasoconstriction."

"Pembelajaran praktik di laboratorium sangat penting bagi peserta didik sebagai sarana pengembangan kompetensi sebelum masuk ke wahana praktik. Selain kompetensi, pembelajaran praktik di laboratorium juga dapat meningkatkan self-efficacy peserta didik agar dapat mempertahankan kompetensinya sebagai perawat. Penelitian ini bertujuan untuk mengembangkan Model Pembelajaran Praktik di Laboratorium yang Efektif (MP2LE) dan menguji efektivitasnya terhadap kompetensi dan self-efficacy peserta didik vokasi keperawatan. MP2LE dibuat berdasarkan model kinerja keterampilan praktik (BjA¸rk & Kirkevold, 2000) dan teori belajar sosial kognitif (Bandura, 1986). Pada penelitian kualitatif, lima tema teridentifikasi, yaitu peran pembimbing, persiapan sebelum praktik, pengelolaan laboratorium yang komprehensif, sarana dan prasarana yang memadai, serta kinerja keterampilan praktik peserta didik. Model MP2LE kemudian dikembangkan berdasarkan hasil integrasi tema yang didapatkan pada penelitian kualitatif, studi literatur, dan konsultasi pakar. Tahap uji coba model menggunakan desain kuasi eksperimen pre-test dan post-test terhadap 89 responden dalam kelompok intervensi dan 84 responden dalam kelompok kontrol. Hasil penelitian menunjukkan terdapat perbedaan yang bermakna dalam hal tingkat pengetahuan, sikap, keterampilan, dan self-efficacy antara kelompok intervensi dan kelompok kontrol dalam waktu dua minggu intervensi. Kesimpulan hasil penelitian yaitu MP2LE efektif dalam meningkatkan kompetensi (pengetahuan, sikap, dan keterampilan) serta self-efficacy peserta didik.

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Laboratory skills learning is really important for nursing student as a way to develop their competency before entering clinical settings. Laboratory skills learning can also improve the students self-efficacy in order to retain their competency as a nurse. This research aims to develop the Laboratory Practical Skills Learning Model (MP2LE) and identify its effectiveness in vocational nursing students competency and self-efficacy. MP2LE is designed by integrating the Practical Skill Performance Model (Bja¸rk & Kirkevold, 2000) and the Social Cognitive Learning Theory (Bandura, 1986). Five themes derived from the qualitative study are the roles of the instructor, preparations before the laboratory skills learning, comprehensive laboratory management, sufficient laboratory facilities, and practical skill performance. MP2LE was then developed based on the qualitative study results, literature reviews, and expert panel. The model testing was held by using quasi-experimental design of pre-test and post-test within an experimental group of 89 participants and a control group of 84 participants. The study showed a significant difference between the two groups during two weeks of intervention in knowledge, attitude, skill, and self-efficacy. This research concludes that MP2LE is effective in improving students competency and self-efficacy."