Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar Belakang: Metilasi di area promoter berpotensi mengakibatkan gene silencing pada gen CDH1 yang berperan penting dalam adhesi antarsel dan morfogenesis kraniofasial. Tujuan: Mengetahui distribusi metilasi antara individu cleft dan non-cleft. Metode: 24 sampel DNA penderita orofacial cleft dan 24 sampel kontrol dianalisis menggunakan teknik methylation-specific PCR (MSP). Hasil: Dari kelompok cleft didapatkan 5 sampel (20,83%) berstatus fully methylated dan 19 sampel (79,17%) berstatus partially methylated, sedangkan dari kelompok kontrol didapatkan 24 sampel (100%) berstatus partially methylated. Kesimpulan: Terjadi metilasi CDH1 pada penderita orofacial cleft, namun secara statistik tidak terdapat perbedaan bermakna pada distribusi status metilasi CDH1 antara individu cleft dan non-cleft (p=0,05).

---

Background: Methylation at promoter area potentially results in silencing of CDH1 gene which plays important role in cell adhesion and craniofacial morphogenesis. Objective: To obtain the distribution of CDH1 methylation in cleft and non-cleft individuals. Methods: 24 DNA samples of individuals with orofacial cleft and 24 control samples were analyzed with methylation-specific PCR (MSP) technique. Results: From cleft group, 5 (20.83%) were fully methylated and 19 (79.17%) were partially methylated; while from control group, 24 (100%) were partially methylated. Conclusion: CDH1 methylation was observed in orofacial cleft affected individuals but there is no significant difference in CDH1 methylation status between cleft and non-cleft individuals (p=0.05).