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Abstrak :
The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation In A Mouse Bone Marrow CultureBisphosphonates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of bisphosphonate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 10$M la, 25-dihydroxyvitamin D3 (la, 25(OH)2 D3) numerous clusters of mononuclear and multinucleated cells formed, which stained positive for tartrate-resistant acid phosphatase (TRAP-positive). la, 25(011)2 D3 is known to stimulate osteoclast-like cell formation in a mouse bone marrow culture. Adding 1-hydroxyethylidene-l, 1-bisphosphonate (BEEP) inhibited the increased formation of osteoclast-like cells stimulated by this stimulant. A time-course experimental model showed that the number of osteoclast-like cells decreased slightly when drugs were given early in the culture period and decreased markedly when the drugs were given later or continuously in the culture period. These findings suggested that bisphosphonate had an effect on mature stage and significantly inhibit bone destruction by inhibit osteoclast-like cells formation. The amount of PGE2 production stimulated by la, 25(011)2 D3 was dose dependently higher with BEEP and 3-amino-lhydroxypropylidene-1, 1-bisphosphonate (APD). Showing that PGE2 production is high at the end of culture when the cells are going to undergo apoptosis. This showed in part, the known bone-resorbing activity of these agents.

Abstrak :
The sensation of pain is the means by which the body is made urgently aware of the presence of tissue damage. Pain represents a protective reflex for self-preservation. It is often pain that brings the patient to the dental office but also can be the factor that keeps the patient from seeking treatment at the appropriate time. Pain control is of great importance in dental practice. The clinician has to know the functional neuroanatomy, peripheral and central nervous system pathways, pain modulating system and various categories of pain of the head, neck and face. Psychological and psychosocial factors also contribute to pain, whether pain arises endogenously from pathologic processes or exogenously from trauma or even dental treatment. The dentists task is threefold: first, to establish the correct diagnosis, second to find the cause of the pain, and third to select the treatment plan that the addresses the patients complaint. By knowing the classification of orofacial pain, the clinician will easily make a diagnosis and determine the cause of the pain. After establishing the physical diagnosis it is easy then to determine the therapy to be made. The treatment in managing dental pain from pharmacotherapy is still effective using analgesic and local anesthetic drug.