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"Salah satu penyebab penurunan produksi karet Indonesia diketahui disebabkan oleh serangan patogen Pestalotiopsis. Salah satu solusi paling relevan dalam mengatasi permasalahan tersebut adalah melalui pembentukan klon karet yang resistan terhadap infeksi Pestalotiopsis. Pembentukan klon karet resistan membutuhkan pemahaman tentang gen-gen terkait pertahanan tanaman. Gen Hevea brasiliensis WRKY40 menjadi salah satu gen pertahanan yang diduga berperan penting dalam memediasi pertahanan tanaman karet. Tujuan penelitian adalah menyajikan informasi mengenai pola peningkatan dan perbedaan ekspresi gen HbWRKY40 pada tanaman karet terhadap infeksi Pestalotiopsis pada klon moderat RRIC 100 dan rentan GT 1. Hipotesis penelitian ini adalah terdapat pola peningkatan ekspresi gen HbWRKY40 pada H. brasiliensis terhadap infeksi Pestalotiopsis dan terdapat perbedaan level ekspresi gen HbWRKY40 pada kedua klon. Penelitian dimulai dengan melakukan ekstraksi RNA daun H. brasiliensis berbasis spin column, yang kemudian diubah menjadi cDNA melalui metode reverse transcriptase polymerase chain reaction (RT-PCR). Sintesis cDNA yang diperoleh digunakan dalam analisis ekspresi gen berbasis quantitative polymerase chain reaction (qPCR) sehingga didapatkan nilai quantification cycle (Cq). Nilai tersebut dihitung menggunakan rumus Livak (2-ΔΔCq) untuk melihat tingkat ekpresi gen dalam satuan perubahan kelipatan (fold change). Hasil penelitian menunjukkan ekspresi gen HbWRKY40 pada tanaman karet yang terinfeksi Pestalotiopsis meningkat pada hari ke-4 dan 6 setelah inokulasi. Selain itu, tingkat ekspresi gen HbWRKY40 pada klon moderat RRIC 100 lebih rendah daripada klon rentan GT 1.

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One of the causes of the decline in Indonesian rubber production is known to be due to attacks by the Pestalotiopsis pathogen. One of the most relevant solutions to this problem is the creation of rubber clones that are resistant to Pestalotiopsis infection. The creation of resistant rubber clones requires an understanding of the genes related to plant defense. The Hevea brasiliensis WRKY40 gene is one of the defense genes that is thought to play an important role in mediating the defense of rubber plants. The objective of this study is to present information on the pattern of increase and differences in the expression of the HbWRKY40 gene in rubber plants against Pestalotiopsis infection in the moderate RRIC 100 and susceptible GT 1 clones. The hypothesis of this study is that there is a pattern of increased expression of the HbWRKY40 gene in H. brasiliensis against Pestalotiopsis infection and that there are differences in the expression levels of the HbWRKY40 gene between the two clones. The study began with RNA extraction from H. brasiliensis leaves using a spin column, which was then converted into cDNA via reverse transcriptase polymerase chain reaction (RT-PCR). The synthesized cDNA was used in gene expression analysis based on quantitative polymerase chain reaction (qPCR), yielding quantification cycle (Cq) values. These values were calculated using the Livak formula (2-ΔΔCq) to determine gene expression levels in terms of fold change. The results showed that the expression of the HbWRKY40 gene in rubber plants infected with Pestalotiopsis sp. increased on days 4 and 6 after inoculation. In addition, the level of HbWRKY40 gene expression in the RRIC 100 moderate clone was lower than that in the GT 1 susceptible clone."