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"Abstract. This article analyses the observation of science & technology (Iptek) and innovation policies in activities of science and technological research, development, and implementation. Science & technology and innovation policies become an important study subject since in some countries this field gives positive effect to competitiveness of a nation. Qualitative approach was selected in studying science & technology and innovation policy in Indonesia since its appropriation with the objective of portraying national policy in science & technology and innovation. Method employed was content analysis qualitative with framing analysis, which in this study refers to concept of science & technological research, development, and implementation. The results of this study shows that national policy of Indonesia during 2000-2011 period were less supportive to development, research, diffusion, and implementation of technology in regions, proven by evidence that there was only few policies supporting the system implementation of science & technological research, development, and implementation in Indonesia.Abstrak. Artikel ini menguraikan tinjauan kebijakan ilmu pengetahuan & teknologi (iptek) dan inovasi dalam kegiatan penelitian, pengembangan, dan penerapan iptek. Kebijakan iptek dan inovasi menjadi penting untuk dikaji karena dibeberapa negara bidang ini memberikan pengaruh terhadap daya saing suatu negara. Pendekatan kualitatif dipilih untuk mengkaji kebijakan iptek dan inovasi di Indonesia karena sesuai dengan tujuan untuk memberikan gambaran mengenai potret kebijakan nasional bidang iptek dan inovasi. Metode yang dipergunakan adalah analisis isi “content analysis” yang bersifat kualitatif dengan pembingkaian “framing analysis” yang dalam studi ini kerangkanya merujuk pada konsep penelitian, pengembangan, dan penerapan iptek. Berdasarkan hasil studi ini terungkap bahwa kebijakan nasional Indonesia selama periode 2000–2011 kurang berpihak pada pengembangan, riset, difusi, dan penerapan teknologi di daerah, terbukti dengan keberadaan kebijakan terkait pelaksanaan sistem penelitian, pengembangan, dan penerapan iptek di Indonesia yang masih sangat minim."

"The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on the imidazole ring, histidine is often used in the IMAC purification system. Two types of commercial IMAC resin use a nitrilotriacetic acid (NTA) matrix: a nickel-based (Ni-NTA) and cobalt-based (Co-NTA), better known as TALON. This study was aim to investigate the effect of the metal ions Ni2+ and Co2+ to purify recombinant human erythropoietin (rhEPO) expressed in yeast system Pichia pastoris. The results indicated that both Ni-NTA and Co-TALON gave almost the same level of protein purity; however, Ni-NTA has a higher binding affinity than Co-TALON might be due to the higher stability complex of Ni+. The average amount of protein bound by Ni-NTA and Co-TALON was 183.5 and 38.7 μg/mL, respectively."

"Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP ishampered by costly experimental method and post-PCR treatment requirment that make this technique is not meetingfor high-throughput analysis purpose. In this study we developed a accurate, simple, low cost and rapid test to detectpolymorphism at HER2 gene using SBR Green I based-melting curve method. Methods: Two forward allele-specificprimers and one common reverse primer were used then these primers were tested to discriminate known genotypes ofgenomic templates (GG type or AA type) and genomic samples retrieved from breast cancer patients. Results: Meltingcurve analysis derived from SYBR Green I-based allele-specific PCR with defined primers concentration and annelingtemperature at 54.3 °C showed good discrimination level of Tm peaks in which GG genotype melted at 89 °C slightlyhigher than AA genotype which melted at 86 °C, while AG genotype harbored both of homozygous Tm characteristics.Conclusions: This preliminary result will be as basic for further large-scale typing of HER2I655V polymorphism."