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Abstrak :
Pendahuluan: Sekretom sel punca mesenkimal dipercaya mengandung faktor pertumbuhan yang bekerja melalui mekanisme parakrin di situs cedera. Di antara banyaknya faktor-faktor pertumbuhan, beberapa disinyalir memiliki efek osteogenik antara lain bone morphogenetic protein-2 (BMP-2), epidermal growth factor (EGF), dan vascular endothelial growth factor (VEGF). Tujuan: Tujuan penelitian ini adalah untuk mengidentifikasi kuantitas BMP-2, EGF dan VEGF pada sekretom sel punca mesenkimal jaringan adiposa dan tali pusat. Metode: Sampel sekretom dari sel punca mesenkimal jaringan adiposa dan tali pusat dibedakan berdasarkan perlakuan pemberian serum atau non serum dan waktu pengambilan saat penggantian medium terakhir atau saat panen, dan dianalisa dengan metode ELISA sandwich assay menggunakan Human BMP-2, VEGF, and EGF ELISA Kits. Hasil: Sebaran nilai BMP-2 tersentrasi pada nilai 0 pada sekretom jaringan adiposa maupun tali pusat. Kadar EGF dan VEGF memiliki perbedaan bermakna pada sampel jaringan adiposa yang berbeda (p<0,009 dan p<0,005). Kadar EGF dan VEGF pada jaringan adiposa adalah 2,67 (0-22,53) dan 1473,5 (136,1-5335) sedangkan pada jaringan tali pusat adalah 2,67 (0-13,29) dan 0 (0-1675). Kesimpulan: Sekretom jaringan adiposa dan tali pusat kemungkinan hanya mengandung BMP-2 dalam nilai yang sangat rendah. Baik jaringan adiposa maupun jaringan tali pusat mengandung EGF dalam jumlah yang moderat. Kadar VEGF pada jaringan adiposa secara signifikan lebih tinggi. ......Background: The secretome derived from mesenchymal stem cells has been suggested contain growth factors that works via a paracrine mechanism in the injured area. Of these factors, some are thought to have an osteogenic effect, including bone morphogenetic protein-2 (BMP-2), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF). Objective: The aim of this study is to identify the quantity of BMP-2, EGF, VEGF in secretome from adipose tissue (AT-MSC) and umbilical cord-derived mesenchymal stem cells (UC-MSC). Methods: Secretome samples from AT-MSC and UC-MSC were grouped based on serum administration and harvesting time, and were analyzed with an ELISA sandwich assay method using Human BMP-2, VEGF, and EGF ELISA Kits. This study aims to identify whether BMP-2 is contained in the secretome of AT-MSC and UC-MSC, which has never been reported before, and to measure the level of EGF and VEGF within the secretome. Results: The distribution of value for BMP-2 was nearly zero in the secretome of AT-MSC and UC-MSC. The level of EGF and VEGF were significantly different between different donor samples of AT-MSC (p<0,009 and p<0,005). The level of EGF and VEGF of AT-MSC are 2,67 (0-22,53) and 1473,5 (136,1-5335) compare to 2,67 (0-13,29) and 0 (0-1675) of UC-MSC. Conclusion: The secretome of AT-MSC and UC-MSC may contain BMP-2 in a very low level. Both AT-MSC and UC-MSC contain EGF in moderate amount. VEGF is significantly higher in of AT-MSC.

Abstrak :
ABSTRAK
Pendahuluan. Osteoarthritis (OA) adalah sebuah penyakit sendi degeneratif yang menyebabkan disabilitas dengan prevalensi yang terus meningkat. Hormon pertumbuhan memiliki efek regenerasi tulang rawan secara langsung melalui stimulasi sel kondroblas dan proses morphoangiogenesis juga melalui faktor pertumbuhan secara sistemik. Penelitian ini bertujuan untuk mengetahui manfaat suntikan sendi dengan hormon pertumbuhan pada kasus Osteoarthritis. Metode Penelitian. Penelitian dilakukan di Rumah Sakit Hewan Institut Pertanian Bogor pada bulan Mei hingga September 2015. Desain penelitian adalah randomized post test only control group. Sejumlah 21 ekor kelinci Selandia Baru putih, berat 1.9-2.6kg, usia 7-8 bulan. Kelinci dibagi secara acak menjadi kelompok kontrol (NaCl 0.9%), suntikan hormon pertumbuhan (4iu), dan suntikan asam hyaluronat (6mg) . Dengan metode acak tersamar dilakukan suntikan kolagenase tipe II C. Histolyticum pada hari 1 dan ke 4 pada lutut kiri, kemudian tindakan penyuntikan dilakukan sebanyak tiga kali dengan selang waktu 1 minggu. Evaluasi dengan periode kepincangan, skoring makroskopis, histologis dilakukan pada minggu ke-8 pasca penyuntikan pertama. Temuan Penelitian. Berdasarkan hasil penelitian ditemukan periode kepincangan pada grup yang diberikan hormon pertumbuhan lebih singkat, dan bermakna secara statistik dibandingkan dengan grup kontrol (p<0.001), grup asam hyaluronat (p<0.03), dan grup hormon pertumbuhan (p<0.001). Evaluasi skor makroskopik dengan skor yoshimi menunjukan bahwa kelompok hormon pertumbuhan memiliki kerusakan tulang rawan yang lebih ringan jika dibandingkan dengan grup kontrol (p=0.001) dan grup asam hyaluronat (p=0.04). Skoring histopatologis menggunakan skor modifikasi Mankin menunjukan pada kelompok dengan hormon pertumbuhan memiliki angka terendah dibandingkan grup lainnya (p=0.001), grup kontrol (p=0.001), grup asam hyaluronat (p=0.015). Kesimpulan. Suntikan hormon pertumbuhan ke dalam sendi memiliki efektifitas yang lebih baik dibandingkan dengan Asam hyaluronat pada model osteoarthritis. Hormon pertumbuhan memberikan harapan baru sebagai alternatif dalam terapi osteoarthritis.ABSTRACT
Introduction. Osteoarthritis is a degenerative joint disorder that cause disability for patients all over the globe, with an increasing number of patients. Growth hormone (GH) works trough direct and indirect effect on cartilage regeneration by chondroblast stimulation, stimulation of growth factors and morphoangiogenesis process. Further research is needed to know the effects of intra articular joint injection of growth hormone using validated animal model and reliable outcome measurement. Methods. This study was conducted in Animal Hospital of Agricultural Institute Bogor west Java, from May to September 2015. The design of the study was randomized posttest only control group. Male white New Zealand rabbit (n=21) weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was randomized and divided into three groups. All groups recieved intra articular injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1 and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline (0.6ml) were done at 2 weeks after collagenase injection once a week for consecutive 3 weeks. Evaluation of weight and lameness periode is done periodically, histopathological and macroscopic score were done at 8 weeks since the first injection. Result. The lameness priode for control group is significantly longer than both of the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001). Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than the HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found lowest at the GH group (p=0.001), with significant difference in control (p=0.001), and HA group (p=0.015).). Conclusion. Intraarticular injection of growth hormone is found to be more effective compared to hyaluronic acid on rabbit osteoarthritis model. This results showed promising result for intra articular injection of GH as an alternative treatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disability for patients all over the globe, with an increasing number of patients. Growth hormone (GH) works trough direct and indirect effect on cartilage regeneration by chondroblast stimulation, stimulation of growth factors and morphoangiogenesis process. Further research is needed to know the effects of intra articular joint injection of growth hormone using validated animal model and reliable outcome measurement. Methods. This study was conducted in Animal Hospital of Agricultural Institute Bogor west Java, from May to September 2015. The design of the study was randomized posttest only control group. Male white New Zealand rabbit (n=21) weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was randomized and divided into three groups. All groups recieved intra articular injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1 and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline (0.6ml) were done at 2 weeks after collagenase injection once a week for consecutive 3 weeks. Evaluation of weight and lameness periode is done periodically, histopathological and macroscopic score were done at 8 weeks since the first injection. Result. The lameness priode for control group is significantly longer than both of the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001). Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than the HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found lowest at the GH group (p=0.001), with significant difference in control (p=0.001), and HA group (p=0.015).). Conclusion. Intraarticular injection of growth hormone is found to be more effective compared to hyaluronic acid on rabbit osteoarthritis model. This results showed promising result for intra articular injection of GH as an alternative treatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disability for patients all over the globe, with an increasing number of patients. Growth hormone (GH) works trough direct and indirect effect on cartilage regeneration by chondroblast stimulation, stimulation of growth factors and morphoangiogenesis process. Further research is needed to know the effects of intra articular joint injection of growth hormone using validated animal model and reliable outcome measurement. Methods. This study was conducted in Animal Hospital of Agricultural Institute Bogor west Java, from May to September 2015. The design of the study was randomized posttest only control group. Male white New Zealand rabbit (n=21) weighted 1.9-2.6kg, age 6-7months were used in this study. The sample was randomized and divided into three groups. All groups recieved intra articular injection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1 and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline (0.6ml) were done at 2 weeks after collagenase injection once a week for consecutive 3 weeks. Evaluation of weight and lameness periode is done periodically, histopathological and macroscopic score were done at 8 weeks since the first injection. Result. The lameness priode for control group is significantly longer than both of the experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001). Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than the HA group (P=0.04) and placebo (P=0.01). Histopathological score was also found lowest at the GH group (p=0.001), with significant difference in control (p=0.001), and HA group (p=0.015).). Conclusion. Intraarticular injection of growth hormone is found to be more effective compared to hyaluronic acid on rabbit osteoarthritis model. This results showed promising result for intra articular injection of GH as an alternative treatment for osteoarthritis.