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"[ABSTRAKLatar belakang: Malignant peripheral nerve sheath tumor (MPNST) adalah sarkoma jaringan lunak yang sulit dibedakan dengan beberapa sarkoma sel spindel karena morfologinya yang serupa. MPNST bersifat agresif dengan angka rekurensi yang tinggi dan cenderung bermetastasis terutama ke paru. Salah satu tahap metastasis adalah invasi dengan cara mendegradasi matriks ekstraseluler dimana Matrix Metalloproteinase (MMP) memainkan peranan penting dalam proses ini. MMP tipe gelatinase yaitu MMP-2 dan MMP-9 memiliki kemampuan dalam mendegradasi membran basal dan kolagen fibrilar sehingga dapat membuka jalur invasi bagi sel tumor. MMP-2 mampu mendegradasi lebih banyak tipe kolagen dan MES non kolagen dibandingkan MMP-9. Penelitian ini bertujuan untuk menilai hubungan antara peningkatan ekspresi MMP-2 dengan derajat keganasan histologik dan variabel prognostik klinis lainnya.Bahan dan cara: Dilakukan pulasan imunohistokimia MMP-2 pada 39 kasus yang terdiri atas 19 MPNST derajat rendah dan 20 MPNST derajat tinggi. Selanjutnya dilakukan analisis hubungan antara peningkatan ekspresi MMP-2 dengan derajat keganasan dan variabel klinis seperti usia, jenis kelamin, ukuran dan lokasi tumor.Hasil: Peningkatan ekspresi MMP-2 ditemukan pada 19 (95%) kasus MPNST derajat tinggi dan 3 kasus (15,8%) kasus MPNST derajat rendah (p=0.000). Terdapat hubungan yang kuat antara peningkatan ekspresi MMP-2 dengan derajat keganasan MPNST. Tidak ditemukan hubungan antara ekspresi MMP-2 dengan jeni usia, jenis kelamin, ukuran dan lokasi tumor.Kesimpulan: Peningkatan ekspresi MMP-2 sejalan dengan peningkatan derajat histologik, sehingga dapat digunakan untuk membantu menentukan progresifitas MPNST.

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ABSTRACTBackground: Malignant peripheral nerve sheath tumor (MPNST) is a sarcoma that is difficult to differentiate with other spindle cell sarcomas, because of their similar morphology. The behavior of MPNST is aggressive, with a high recurrence and tend to metastases hematogenous, especially to lung. Important step of metastases is invasion by degradating extracellular matrix, in which Matrix Metalloproteinase (MMP) play important role in this process. Gelatinase type of MMP, MMP-2 and MMP-9 have ability to degrade basal membrane and fibriler collagen, in order to open the way of invasion. MMP-2 can degrade type collagen and non collagen extracellular matrix than MMP-9. The aim of this study is to see the correlation between expression of MMP-2 and histopathology grading and other prognostic clinical variables.Methods: This study enrolled 39 cases of consisted of 19 low grade MPNST and 20 high grade MPNST. The case were stained for MMP-2 imunnohistochemistry and the expression of MMP-2 were scored. Analysis the correlation between over expression of MMP-2 and histopathology grading and other clinical variables , such as age, sex, size and location of the tumor.Results: Overexpression of MMP-2 was observed in 19 (95%) cases of high grade MPNST and 3 (15,8%) cases of low grade MPNST (p=0.000). There is a significant correlation between MMP-2 over expression and histopathology grade. There is no correlation between MMP-2 expression and age, sex, siize and location of tumor.Conclusion: High expression of MMP-2 is in parallel with high histologic grade, therefore it may be of additional use as prognostic factor., ackground: Malignant peripheral nerve sheath tumor (MPNST) is a sarcoma that is difficult to differentiate with other spindle cell sarcomas, because of their similar morphology. The behavior of MPNST is aggressive, with a high recurrence and tend to metastases hematogenous, especially to lung. Important step of metastases is invasion by degradating extracellular matrix, in which Matrix Metalloproteinase (MMP) play important role in this process. Gelatinase type of MMP, MMP-2 and MMP-9 have ability to degrade basal membrane and fibriler collagen, in order to open the way of invasion. MMP-2 can degrade type collagen and non collagen extracellular matrix than MMP-9. The aim of this study is to see the correlation between expression of MMP-2 and histopathology grading and other prognostic clinical variablesMethods: This study enrolled 39 cases of consisted of 19 low grade MPNST and 20 high grade MPNST. The case were stained for MMP-2 imunnohistochemistry and the expression of MMP-2 were scored. Analysis the correlation between over expression of MMP-2 and histopathology grading and other clinical variables , such as age, sex, size and location of the tumor.Results:. Overexpression of MMP-2 was observed in 19 (95%) cases of high grade MPNST and 3 (15,8%) cases of low grade MPNST (p=0.000). There is a significant correlation between MMP-2 over expression and histopathology grade. There is no correlation between MMP-2 expression and age, sex, siize and location of tumor.Conclusion: High expression of MMP-2 is in parallel with high histologic grade, therefore it may be of additional use as prognostic factor.]"

"[ABSTRAKLatar Belakang: Penggunaan biomaterial berupa bahan tandur tulang dan membran untuk prosedur Guided Bone Regeneration (GBR) sangat diperlukan di bidang bedah maksilofasial dan, untuk mengatasi defek tulang yang dapat terjadi oleh berbagai sebab. Penelitian ini bertujuan untuk mengetahui efek pemakaian bahan tandur tulang DFDBX dengan membran perikardium (MPK) bovine pada defek tulang kalvaria tikus.Bahan dan Metode: Studi eksperimental ini menggunakan 45 ekor tikus Sprague Dawley sebagai hewan coba dibagi dalam 3 kelompok secara acak. Ciritical size defect sebesar diameter 5 mm dibuat pada tulang kalvaria seluruh hewan coba. Kelompok I merupakan kelompok kontrol, tidak diberikan perlakuan dan defek dibiarkan sembuh dengan sendirinya, kelompok II yang diberi DFDBX, dan pada kelompok III defek diisi dengan DFDBX dan ditutup dengan MPK (DFDBX+MPK). Setelah 1,4 dan 8 minggu dilakukan dilakukan pengorbanan pada kelompok hewan coba, dilanjutkan dengan evaluasi secara radiologik, histopatologik untuk reaksi radang, pertumbuhan tulang dan pemeriksaan imunohistokimia dengan osteokalsin. Data dianalisis secara statistik dengan menggunakan uji ANOVA.Hasil: Penilaian radiografik diperoleh perbedaan bermakna pada rerata densitas area defek minggu ke 8 antara kelompok kontrol dengan DFDBX+MPK (p<0,001) dan antara kelompok DFDBX dengan DFDBX+MPK (p=0,03).Pertumbuhan tulang baru pada minggu ke 8 tertinggi adalah pada kelompok DFDBX+MPK dengan perbedaan bermakna dengan kelompok kontrol (p=0,016) dan dengan kelompok DFDBX nilai p=0,048. Ekspresi osteokalsin minggu ke-8 menunjukkan perbedaan bermakna antara kelompok kontrol dengan kelompok DFDBX (p<0,001) maupun dengan kelompok DFDBX+MPK (p=0,0013), namun tidak terdapat perbedaan bermakna antara kelompok DFDBX dengan kelompok MPK (p=1,000).Kesimpulan: Penggunaan DFDBX dengan kombinasi MPK terbukti secara radiologik, histopatologik dan imunohistokimia dapat meningkatkan regenerasi tulang pada defek tulang kalvaria.

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ABSTRACTBackground: Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has included bone grafting and using membrane in guided bone regeneration procedure. Using biomaterial such as bone grafting and membrane for Guided Bone Regeneration (GBR) procedures is an essential issue in maxillofacial and dental reconstruction surgery to overcome bone defects caused by various etiologies. Our study was aimed to identify the effect of using Demineralized Freeze-Dried Bone Xenograft (DFDBX) with (or without) bovine pericardium membrane (PCM) on the treatment of rats calvarial bone defects.Materials and Method: The experimental study used 45 Sprague-Dawley rats as the experimental animals, which were categorized randomly into three groups, i.e. the control group, DFDBX group, and DFDBX+PCM group. The 5-mm-critical-sized calvarial defects were created in all experimental animals. The first group was a control group, which did not receive any treatment with self-limiting defects; while subjects in the second group received DFDBX (DFDBX group) and in the third group, the defects were filled with DFDBX and PCM (DFDBX + PCM group). Animals were sacrified at the 1st, 4th, and 8th weeks following the surgery. Subsequently, an evaluation was carried out using radiological analysis, histopathological assay to observe inflammatory reaction and bone growth, as well as immunohistochemical analysis of osteocalcin. Data were analyzed statistically using ANOVA test. The specimens were embedded ini paraffin, serially cut, and stained with hematoxylin and eosin for analysis under light microscope. The inflammation reaction, new bone formation, and the rest of DFDBX and PCM were histomorphometrically evaluated. Immunohistochemical analysis of osteocalcin expression was performed.Results: Radiological analysis demonstrated a significant difference of mean bone density in the defect area at the 8th week between subjects in the control group and those in DFDBX+PCM group (p < 0.001), as well as between subjects in the DFDBX group and those in DFDBX+PCM group (p = 0.03). The highest rate of bone healing at the 8th week was found in DFDBX+PCM group, which showed significant difference compared to the control group (p=0.016) and to DFDBX group (p=0.048). There was a significant difference of osteocalcin expression between the control group and DFDBX group (p < 0.001), as well as between the control group and DFDBX + PCM group (p=0,0013). However, there was no significant difference between the DFDBX group and the DFDBX+PCM group (p = 1.000). Conclusion: Our radiological, histopahtological and immunohistochemical evaluation has demonstrated that DFDBX combined with PCM increases bone regeneration in the treatment of bone calvarial defect. ;Background :Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has been bone grafting and using membrane in guided boneregeneration procedure.The aim of this study was to analyze the effect of Demineralized Freeze Dried BoneXenograft (DFDBX) with (or without)bovine pericardium membrane (PCM) on boneregeneration, in surgically created critical-size defects in rat calvaria, radiographically,histopathologically and immunohistochemically.Material and Methods :Surgical critical-size bone defects were created in 45 animalsthat randomly divided into three groups : control group, DFDBX group, andDFDBX+PCM group. Animals were sacrified at 1, 4 and 8 weeks post surgery.Radiological analysis was done. The specimens were embedded ini paraffin, serially cut,and stained with hematoxylin and eosin for analysis under light microscope. Theinflammation reaction, new bone formation, and the rest of DFDBX and PCM werehistomorphometrically evaluated. Immunohistochemical analysis of osteocalcinexpression was performed. Result : DFDBX and DFDBX+PCM groups demonstrated superior bone healingcompared with control group. Group DFDBX+PCM showmore advanced healing at 8weeks post surgery and show the highest density radiographically as compared with theother group DFDBX and control.Immunohistochemistry revealed the presence ofosteocalcin in osteoblast and matrix extracellular and show significant differences werenoted between DFDBX and DFDBX+PCM to control groups.Conclusion : Application of DFDBX combined with bovine PCM gave the best result in bone regeneration of critical size defects in rat calvaria. , Background :Reconstruction of cranial and maxillofacial defects is a challenging task. The standard method has been bone grafting and using membrane in guided boneregeneration procedure.The aim of this study was to analyze the effect of Demineralized Freeze Dried BoneXenograft (DFDBX) with (or without)bovine pericardium membrane (PCM) on boneregeneration, in surgically created critical-size defects in rat calvaria, radiographically,histopathologically and immunohistochemically.Material and Methods :Surgical critical-size bone defects were created in 45 animalsthat randomly divided into three groups : control group, DFDBX group, andDFDBX+PCM group. Animals were sacrified at 1, 4 and 8 weeks post surgery.Radiological analysis was done. The specimens were embedded ini paraffin, serially cut,and stained with hematoxylin and eosin for analysis under light microscope. Theinflammation reaction, new bone formation, and the rest of DFDBX and PCM werehistomorphometrically evaluated. Immunohistochemical analysis of osteocalcinexpression was performed. Result : DFDBX and DFDBX+PCM groups demonstrated superior bone healingcompared with control group. Group DFDBX+PCM showmore advanced healing at 8weeks post surgery and show the highest density radiographically as compared with theother group DFDBX and control.Immunohistochemistry revealed the presence ofosteocalcin in osteoblast and matrix extracellular and show significant differences werenoted between DFDBX and DFDBX+PCM to control groups.Conclusion : Application of DFDBX combined with bovine PCM gave the best result in bone regeneration of critical size defects in rat calvaria. ]"

"[ABSTRAKLatar belakang: Neoplasma sel plasma (NSP) adalah proliferasi sel plasma neoplastik yang tumbuh soliter menjadi plasmasitoma tulang soliter (PTS) dan plasmasitoma ekstrameduler (PEM) serta multipel (MM)). Saat ini perjalanan penyakit dari plasmasitoma menjadi MM sulit diprediksi. Bartl mengklasifikasikan derajat keganasan berdasarkan histomorfologik menjadi rendah, sedang dan tinggi. Penelitian ini bertujuan menggunakan klasifikasi Bartl untuk menilai perjalanan PTS dan PEM menjadi MM dihubungkan dengan ekspresi TP53 dan Ki-67.Bahan dan cara: Pada 32 kasus NSP yang berasal dari PTS 14 kasus, PEM 5 kasus, maupun MM sebanyak 13 kasus, diklasifikasikan menjadi 3 kelompok derajat keganasan menurut Bartl yaitu derajat keganasan ringan, sedang dan tinggi. Selanjutnya dilakukan pulasan IHK TP53 dan Ki-67 pada seluruh kasus dan dihitung persentase positifitas.Hasil: Berdasarkan derajat keganasan, derajat rendah ditemukan pada 10 (31,2%) MM, derajat sedang pada 5 (15,6%) PTS dan derajat tinggi pada 2 (6,2%) PTS dan PEM. Peningkatan ekspresi TP53 ditemukan pada derajat Bartl yaitu median derajat rendah 4%, derajat sedang 16%, dan derajat tinggi 10%. Terdapat perbedaan ekspresi TP53 yang bermakna antara derajat keganasan rendah dan sedang (p=0,004). Rerata indeks proliferasi Ki-67 pada derajat keganasan rendah 57%, derajat sedang 44,6%, dan derajat tinggi 32,6%. Tidak ditemukan perbedaan antara indeks proliferasi Ki-67 dengan derajat keganasan menurut Bartl (p=0,339). Tidak terdapat hubungan antara ekspresi TP53, Ki-67 dengan usia. Kesimpulan: Peningkatan ekspresi TP53 pada NSP sejalan dengan peningkatan derajat keganasan Bartl, terutama pada derajat rendah dan sedang. Klasifikasi Bartl ditambah dengan pulasan TP53 saja tidak cukup untuk memprediksi perkembangan PTS dan PEM menuju MM.

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ABSTRACTBackground: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl's classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl's degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl's low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl's degrees of malignancy, especially on low and inter.;Background: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl?s classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl?s degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl?s low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl?s degrees of malignancy, especially on low and inter, Background: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl’s classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl’s degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl’s low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl’s degrees of malignancy, especially on low and inter]"

"Latar belakang: Interpretasi cairan peritoneum yang tepat secara sitopatologi sangat mempengaruhi tatalaksana dan prognosis pasien, padahal pemeriksaan sitopatologi cairan peritoneum masih memiliki nilai negatif palsu dan positif palsu yang cukup tinggi, dan hingga saat ini penelitian tentang arsitektur sitopatologi maupun penanda sitomorfologi yang mengarahkan pada adanya sel neoplasma di cairan peritoneum masih menunjukkan hasil yang beragam. Bahan dan cara kerja: Penelitian potong lintang dengan data sekunder berupa slaid dan formulir sediaan sitopatologi cairan peritoneum yang memiliki data berpasangan dengan diagnosis histopatologi. Diagnosis klinis berupa neoplasma epitelial ovarium. Slaid dan formulir diambil dari arsip Departemen Patologi Anatomik FKUI/RSCM tahun 2011 - 2012, dilakukan pembacaan ulang semua slaid sitopatologi dengan diagnosis akhir dikategorikan sebagai positif atau negatif, peneliti membaca pula sediaan histopatologi untuk mengetahui morfologi sel pada lesi, kemudiaan dilakukan penilaian terhadap arsitektur sitopatologi berupa: selularitas, sel berkelompok, struktur papiler, intercelular windows, group contours, jisim psamoma, dan penanda sitomorfologi berupa: atipia inti, inti bertumpuk, anak inti, rasio inti:sitoplasma, ukuran inti, dan ukuran sel.Hasil penelitian: Sampel penelitian sejumlah 47 sediaan sitopatologi dengan diagnosis sitopatologi akhir 34 kasus (72.3%) negatif, 13 kasus (27.7%) positif. Terdapat perbedaan bermakna arsitektur sitopatologi berupa: selularitas (p = 0.017), sel berkelompok (p = 0.001), intercellular windows (p = 0.00), group contours (p = 0.00), dan gambaran sitomorfologi berupa: atipia inti (p = 0.00), inti bertumpuk (p = 0.001), anak inti (p = 0.001), rasio inti:sitoplasma (p = 0.00), ukuran inti (p = 0.00), ukuran sel (p = 0.00) antara cairan peritoneum positif dan negatif. Melalui uji multivariat didapatkan penanda yang paling berpengaruh terhadap diagnosis sitopatologi positif atau negatif yaitu: intercellular windows, atipia inti, dan selularitas.Kesimpulan: Terdapat tiga penanda yang paling berpengaruh terhadap diagnosis positif ditemukannya sel neoplasma ganas dalam cairan peritoneum pada kasus dengan lesi ovarium, secara berturut - turut yaitu: tidak ditemukannya intercellular windows pada kelompokan sel, sel memiliki atipia inti sedang hingga berat, dan selularitas lebih dari 20 kelompok dari keseluruhan sediaan apus.

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Background : Peritoneal fluid cytopathology interpretation profoundly influences patients management and prognosis, however this practice still has high false positive and false negative value, and until now research concerning the architectural and cytomorphology features for detecting malignant cells in peritoneal fluid still has various result.Materials and Methods : Cross sectional study using secondary data of peritoneal fluid cytopathology and histopathology slides and form, from patients with clinical diagnosis of ovarian epithelial neoplasm. The data was taken from the archive of Anatomical Pathology Department Cipto Mangunkusumo Hospital 2011 - 2012. The researchers examined the cytopathology slides and also examined the histopatology slide for morphology comparison, and then make a final cytopathological diagnosis of positive peritoneal fluid containing neoplastic cells or negative. Architectural features including: cellularity, cells grouping, papillary structure, intercellular windows, group contours, psamoma bodies, and cytomorphology features including: nuclear atypia, overlapping nuclei, nucleoli, nuclei : cytoplasm ratio, the dimension of the nuclei and cells were also examined.Result : There were 47 samples with final cytopathology diagnosis: 34 cases (72.3%) negative for neoplastic cells in the peritoneal fluid and 13 cases (27.7%) positive. There were significant differences in cytopathology architectural including cellularity (p = 0.017), cells grouping (p = 0.001), intercellular windows (p = 0.00), group contours (p = 0.00) and cytomorphology features including nuclear atypia (p = 0.00), overlapping nuclei (p = 0.001), nucleoli (p =0.001), nuclei : cytoplasm ratio (p = 0.00), the dimension of nuclei (p = 0.00), the dimension of cell (p = 0.00) between the positive and negative peritoneal fluid cytopathology. Using multivariate analysis there were 3 cytological features that have the strongest association with positive or negative peritoneal cytopathology diagnosis, they were: intercellular windows, nuclear atypia, and cellularity.Conclusion: In peritoneal fluid cytopathology for examining ovarian lesion there were 3 cytological features that have the strongest association with finding neoplastic cells in peritoneal fluid, they were: the absent of intercellular windows, moderate to severe cytological atypia, and cellularity more than 20 groups in all smear preparation."

"ABSTRAK Latar belakang: Fibroadenoma dan tumor filodes jinak merupakan tumorfibroepitelial dengan gambaran histopatologik yang tumpang tindih. Saat inibanyak pengambilan jaringan tumor payudara secara core biopsy, termasuk padatumor fibroepitelial. Jumlah jaringan yang sedikit dan gambaran histopatologikyang tumpang tindih sering menyulitkan Dokter Spesialis Patologi Anatomikdalam menentukan diagnosis fibroadenoma dan tumor filodes jinak. Penelitian inibertujuan untuk mengetahui gambaran histopatologik apa saja yang bermaknauntuk mendiagnosis fibroadenoma dan tumor filodes jinak dan untuk mengujiapakah diagnosis fibroadenoma dan tumor filodes jinak pada core biopsy denganmenggunakan sistem skoring lebih baik dibandingan tanpa skoring.Bahan dan cara: Penelitian ini merupakan suatu uji diagnostik. 57 kasusfibroadenoma dan tumor filodes jinak yang memiliki slaid core biopsy danmastektomi/lumpektomi/eksisi dinilai ulang tanpa sistem skoring danmenggunakan skoring. Gambaran histopatologik yang dinilai pada sistem skoringadalah selularitas stroma, atipia inti, fragmentasi jaringan, infiltrasi lemak, mitosisdan heterogenitas stroma. Kemudian dilakukan analisis statistik, uji diagnostikdan uji kappa.Hasil: Selularitas stroma, heterogenitas stroma dan fragmentasi jaringan lebihsering ditemukan pada tumor filodes jinak dan berbeda bermakna (p=0,001;p=0,000; p=0,021). Spesifisitas pada sistem skoring meningkat sebesar 17,9%.Nilai duga positif dan nilai duga negatif pada sistem skoring meningkat sebesar11,9% dan 5,1%. Area under curve (AUC) meningkat 8,9%. Uji Cohen?s kappaantara diagnosis core biopsy tanpa dan dengan skoring bernilai rendah (0,545).Kesimpulan: Adanya peningkatan spesifisitas, nilai duga positif dan AUCmenunjukkan bahwa penilaian core biopsy sistem skoring lebih baikdibandingkan tanpa skoring dan dapat menjadi acuan untuk diagnosis fibroadenoma dan tumor filodes jinak.ABSTRACT Background: Fibroadenoma and benign phyllodes tumor are kinds of fibroepithelial tumor which have overlapping histopathological features. Recently, core biopsy is commonly performed to determine breast tumor, including fibroepithelial tumor. Small amount of tissue and overlapped histopathological features often complicate the Pathologist in diagnosing both. This study aims to describe the histopathological appearance which needed to diagnose fibroadenoma and benign phyllodes tumor and to verify if the diagnosis of fibroadenoma and benign phyllodes tumor in core biopsy using scoring system is more accurate than without scoring system.Method: This study was a diagnostic test, in which 57 cases of fibroadenoma and benign phyllodes tumor which had undergone core biopsy and mastectomy/excision were re-assessed using and without using scoring system. Histopathologic features which assessed using scoring system were stromal cellularity, nuclear atypia, tissue fragmentation, fat infiltration, mitotic figure, stromal heterogeneity. Analytical statistic, diagnostic test, accuracy test and Kappa test were done.Results: Stromal cellularity, stromal heterogeneity and tissue fragmentation mostly found in benign phyllodes tumor and significantly different (p=0,001; p=0,000; p=0,021).There were significant differences between stromal cellularity (p=0,001), stromal heterogeneity (p=0,000), and tissue fragmentation (p=0,021) in diagnosis of benign phyllodes tumor. Specificity in scoring system increased by17,9 %. Positive predictive value, negative predictive value and accuracy increased in scoring system (11,9% and 5,1%). Area under curve (AUC) increased by 8,9%. Cohen's Kappa test between core biopsy diagnosis without using and using scoring system had low result(0,545). Conclusion: The increasing of specificity, positive predictive value, accuracy and AUC proved that core biopsy with scoring system is more accurate than without scoring. This can be used as reference to diagnose fibroadenoma and benign phyllodes tumor.;Background: Fibroadenoma and benign phyllodes tumor are kinds of fibroepithelial tumor which have overlapping histopathological features. Recently, core biopsy is commonly performed to determine breast tumor, including fibroepithelial tumor. Small amount of tissue and overlapped histopathological features often complicate the Pathologist in diagnosing both. This study aims to describe the histopathological appearance which needed to diagnose fibroadenoma and benign phyllodes tumor and to verify if the diagnosis of fibroadenoma and benign phyllodes tumor in core biopsy using scoring system is more accurate than without scoring system.Method: This study was a diagnostic test, in which 57 cases of fibroadenoma and benign phyllodes tumor which had undergone core biopsy and mastectomy/excision were re-assessed using and without using scoring system. Histopathologic features which assessed using scoring system were stromal cellularity, nuclear atypia, tissue fragmentation, fat infiltration, mitotic figure, stromal heterogeneity. Analytical statistic, diagnostic test, accuracy test and Kappa test were done.Results: Stromal cellularity, stromal heterogeneity and tissue fragmentation mostly found in benign phyllodes tumor and significantly different (p=0,001; p=0,000; p=0,021).There were significant differences between stromal cellularity (p=0,001), stromal heterogeneity (p=0,000), and tissue fragmentation (p=0,021) in diagnosis of benign phyllodes tumor. Specificity in scoring system increased by17,9 %. Positive predictive value, negative predictive value and accuracy increased in scoring system (11,9% and 5,1%). Area under curve (AUC) increased by 8,9%. Cohen's Kappa test between core biopsy diagnosis without using and using scoring system had low result(0,545). Conclusion: The increasing of specificity, positive predictive value, accuracy and AUC proved that core biopsy with scoring system is more accurate than without scoring. This can be used as reference to diagnose fibroadenoma and benign phyllodes tumor.;Background: Fibroadenoma and benign phyllodes tumor are kinds of fibroepithelial tumor which have overlapping histopathological features. Recently, core biopsy is commonly performed to determine breast tumor, including fibroepithelial tumor. Small amount of tissue and overlapped histopathological features often complicate the Pathologist in diagnosing both. This study aims to describe the histopathological appearance which needed to diagnose fibroadenoma and benign phyllodes tumor and to verify if the diagnosis of fibroadenoma and benign phyllodes tumor in core biopsy using scoring system is more accurate than without scoring system.Method: This study was a diagnostic test, in which 57 cases of fibroadenoma and benign phyllodes tumor which had undergone core biopsy and mastectomy/excision were re-assessed using and without using scoring system. Histopathologic features which assessed using scoring system were stromal cellularity, nuclear atypia, tissue fragmentation, fat infiltration, mitotic figure, stromal heterogeneity. Analytical statistic, diagnostic test, accuracy test and Kappa test were done.Results: Stromal cellularity, stromal heterogeneity and tissue fragmentation mostly found in benign phyllodes tumor and significantly different (p=0,001; p=0,000; p=0,021).There were significant differences between stromal cellularity (p=0,001), stromal heterogeneity (p=0,000), and tissue fragmentation (p=0,021) in diagnosis of benign phyllodes tumor. Specificity in scoring system increased by17,9 %. Positive predictive value, negative predictive value and accuracy increased in scoring system (11,9% and 5,1%). Area under curve (AUC) increased by 8,9%. Cohen's Kappa test between core biopsy diagnosis without using and using scoring system had low result(0,545). Conclusion: The increasing of specificity, positive predictive value, accuracy and AUC proved that core biopsy with scoring system is more accurate than without scoring. This can be used as reference to diagnose fibroadenoma and benign phyllodes tumor.;Background: Fibroadenoma and benign phyllodes tumor are kinds of fibroepithelial tumor which have overlapping histopathological features. Recently, core biopsy is commonly performed to determine breast tumor, including fibroepithelial tumor. Small amount of tissue and overlapped histopathological features often complicate the Pathologist in diagnosing both. This study aims to describe the histopathological appearance which needed to diagnose fibroadenoma and benign phyllodes tumor and to verify if the diagnosis of fibroadenoma and benign phyllodes tumor in core biopsy using scoring system is more accurate than without scoring system.Method: This study was a diagnostic test, in which 57 cases of fibroadenoma and benign phyllodes tumor which had undergone core biopsy and mastectomy/excision were re-assessed using and without using scoring system. Histopathologic features which assessed using scoring system were stromal cellularity, nuclear atypia, tissue fragmentation, fat infiltration, mitotic figure, stromal heterogeneity. Analytical statistic, diagnostic test, accuracy test and Kappa test were done.Results: Stromal cellularity, stromal heterogeneity and tissue fragmentation mostly found in benign phyllodes tumor and significantly different (p=0,001; p=0,000; p=0,021).There were significant differences between stromal cellularity (p=0,001), stromal heterogeneity (p=0,000), and tissue fragmentation (p=0,021) in diagnosis of benign phyllodes tumor. Specificity in scoring system increased by17,9 %. Positive predictive value, negative predictive value and accuracy increased in scoring system (11,9% and 5,1%). Area under curve (AUC) increased by 8,9%. Cohen's Kappa test between core biopsy diagnosis without using and using scoring system had low result(0,545). Conclusion: The increasing of specificity, positive predictive value, accuracy and AUC proved that core biopsy with scoring system is more accurate than without scoring. This can be used as reference to diagnose fibroadenoma and benign phyllodes tumor."

"ABSTRAK Latar Belakang :Karsinoma kolorektal (KKR) merupakan penyebab kematian kedua di dunia dari seluruh jeniskanker. KKR dapat disebabkan oleh defek dari MMR DNA. Microsatellite instability (MSI)adalah penanda defek MMR DNA. KKR MSI-H memiliki gambaran karakteristik tertentu.Tumor-infiltrating-lymphocyte (TIL) merupakan faktor prognosis. Hilangnya ekspresi PMS2 danMSH6 dapat sebagai penanda MSI. Penelitian ini bertujuan untuk menilai terjadinya MSI padaKKR di sisi kiri dan sisi kanan kolon melalui Hilangnya ekspresi PMS2 dan MSH6, sertamengetahui hubungan antara TIL dengan MSI-H.Bahan dan Metode :Dilakukan pulasan IHK PMS2 dan MSH6, serta penghitungan TIL. Penilaian dilakukan denganmenghitung hilangnya ekspresi PMS2 dan MSH6 pada inti sel dan dikelompokkan ke dalamkelompok mutasi dan tidak mutasi .Penghitungan TIL juga dikelompokkan ke dalam TIL tinggidan rendah, berdasarkan nilai titik potong Hasil : Didapatkan 27,8% kasus menunjukkan hilangnya ekspresi PMS2 dan MSH6 dengan 14,4%kasus di distal kolon. TIL terbanyak di distal kolon 30% kasus. Tidak terdapat perbedaanbermakna antara mutasi PMS2 dan MSH6 dengan lokasi (p=0,829) dan TIL (p=0,187). Terdapatperbedaan bermakna antara usia dan lokasi (p=0,020) serta peningkatan ekspresi PMS2 denganMSH6 (p=0,06).Kesimpulan :MSI-H ditemukan pada 27,8% kasus. Penggunaan PMS2 dan MSH6 pada penelitian ini belumdapat menggantikan 4 panel IHK. Terdapat kecenderungan dimana adenokarsinoma NOSmemiliki frekuensi mutasi lebih tinggi dari adenokarsinoma musinosum.ABSTRACT Background : Colorectal carcinoma (CRC) is the world second leading cause of death from all types of cancer.CRC can be caused by a defect of MMR DNA. Microsatellite instability (MSI) is a marker ofDNA MMR defect. CRC MSI-H has a certain characteristic figures. Tumor-infiltratinglymphocytes (TIL) isone of prognostic factor. Loss expression of the PMS2 and MSH6 can beuse as a marker of MSI. This study aims to assess the occurrence of MSI in CRC on the left sideand the right side of the colon through the loss of expression of PMS2 and MSH6, anddetermine the relationship between TIL with MSI-H. Materials and Methods :Immunohistochemical staining using two marker, there is PMS2 and MSH6. We also countingthe number of TIL. Assessment by calculating the loss expression of PMS2 and MSH6 in the cellnuclei and divided into two groups, the mutations and non mutations . TIL result also groupedinto high and low, based on the cutoff point. Result :There are 27.8% of cases showed loss of expression of PMS 2 and MSH6 with 14.4% of cases inthe distal colon. About 30% TIL cases located in distal colon. There were no significantdifferences between PMS2 and MSH6 mutation with the location (p = 0.829) and TIL (p =0.187). There are significant differences between age and location (p = 0.020) and increasedexpression of PMS2 with MSH6 (p = 0.06). \ Conclusion :MSI-H was found in 27.8% of cases. The use of PMS2 and MSH6 in this study have not beenable to replace 4 panels of IHC. There is a tendency where the adenocarcinoma NOS have ahigher mutation frequency than mucinous adenocarcinoma. ;Background :Colorectal carcinoma (CRC) is the world second leading cause of death from all types of cancer.CRC can be caused by a defect of MMR DNA. Microsatellite instability (MSI) is a marker ofDNA MMR defect. CRC MSI-H has a certain characteristic figures. Tumor-infiltratinglymphocytes (TIL) isone of prognostic factor. Loss expression of the PMS2 and MSH6 can beuse as a marker of MSI. This study aims to assess the occurrence of MSI in CRC on the left sideand the right side of the colon through the loss of expression of PMS2 and MSH6, anddetermine the relationship between TIL with MSI-H. Materials and Methods :Immunohistochemical staining using two marker, there is PMS2 and MSH6. We also countingthe number of TIL. Assessment by calculating the loss expression of PMS2 and MSH6 in the cellnuclei and divided into two groups, the mutations and non mutations . TIL result also groupedinto high and low, based on the cutoff point. Result :There are 27.8% of cases showed loss of expression of PMS 2 and MSH6 with 14.4% of cases inthe distal colon. About 30% TIL cases located in distal colon. There were no significantdifferences between PMS2 and MSH6 mutation with the location (p = 0.829) and TIL (p =0.187). There are significant differences between age and location (p = 0.020) and increasedexpression of PMS2 with MSH6 (p = 0.06). \ Conclusion :MSI-H was found in 27.8% of cases. The use of PMS2 and MSH6 in this study have not beenable to replace 4 panels of IHC. There is a tendency where the adenocarcinoma NOS have ahigher mutation frequency than mucinous adenocarcinoma. ;Background :Colorectal carcinoma (CRC) is the world second leading cause of death from all types of cancer.CRC can be caused by a defect of MMR DNA. Microsatellite instability (MSI) is a marker ofDNA MMR defect. CRC MSI-H has a certain characteristic figures. Tumor-infiltratinglymphocytes (TIL) isone of prognostic factor. Loss expression of the PMS2 and MSH6 can beuse as a marker of MSI. This study aims to assess the occurrence of MSI in CRC on the left sideand the right side of the colon through the loss of expression of PMS2 and MSH6, anddetermine the relationship between TIL with MSI-H. Materials and Methods :Immunohistochemical staining using two marker, there is PMS2 and MSH6. We also countingthe number of TIL. Assessment by calculating the loss expression of PMS2 and MSH6 in the cellnuclei and divided into two groups, the mutations and non mutations . TIL result also groupedinto high and low, based on the cutoff point. Result :There are 27.8% of cases showed loss of expression of PMS 2 and MSH6 with 14.4% of cases inthe distal colon. About 30% TIL cases located in distal colon. There were no significantdifferences between PMS2 and MSH6 mutation with the location (p = 0.829) and TIL (p =0.187). There are significant differences between age and location (p = 0.020) and increasedexpression of PMS2 with MSH6 (p = 0.06). \ Conclusion :MSI-H was found in 27.8% of cases. The use of PMS2 and MSH6 in this study have not beenable to replace 4 panels of IHC. There is a tendency where the adenocarcinoma NOS have ahigher mutation frequency than mucinous adenocarcinoma. "

"ABSTRAK Latar belakang: Endometriosis merupakan kelainan ginekologik yang palingsering ditemukan. Seperti halnya endometrium di uterus juga dapat terjadiberbagai perubahan pada epitel yang melapisi kista endometriosis di ovarium,antara lain metaplasia, hiperplasia, atipia bahkan perubahan ke arah keganasan.Saat ini banyak penelitian yang menghubungkan antara endometriosis dan kankerovarium terutama jenis clear cell dan dikenal dengan istilah endometriosisassociatedovarian carcinoma (EAOC) dan dilaporkan adanya mutasi yangmenginaktifkan gen supresor tumor (ARID1A), sehingga protein BAF250a tidakdiekpresikan pada Clear cell carcinoma (CCC) ovarii.Bahan dan cara: Dilakukan pulasan imunohistokimia ARID1A pada sampel 20kasus endometriosis non atipik, 20 kasus atipik dan 20 kasus CCC ovarii tahun2012 hingga Maret 2015. Dari kelompok kasus CCC didapatkan 9 kasus EAOC.Selanjutnya dilihat adakah perbedaan persentase ekspresi ARID1A padaendometriosis non atipik, atipik, CCC ovarii serta endometriosis disertai CCC(EAOC).Hasil: Pada kelompok kasus endometriosis non atipik, atipik dan CCC adaperbedaan bermakna persentase ekspresi ARID1A (uji Kruskal-Wallis p=0,0035).Selanjutnya dilakukan analisis Post Hoc uji Mann-Whitney dan didapatkanperbedaan bermakna persentase ekspresi ARID1A antara endometriosis non atipikdan atipik dengan CCC ovarii (p=0,001 dan p=0,0015). Pada kelompok kasusendometriosis non atipik, atipik dan endometriosis pada EAOC, didapatkan adaperbedaan bermakna persentase ekspresi ARID1A (Uji Kruskal-Walis p=0,011).Selanjutnya dilakukan analisis Post Hoc uji Mann-Whitney dan ada perbedaanbermakna persentase ekspresi ARID1A antara endometriosis non atipik dan atipikdengan EAOC (p=0,005 dan p=0,008). Kesimpulan: Ekspresi ARID1A pada endometriosis non atipik dan atipik lebihtinggi bermakna dibanding CCC ovarii dan EAOC. Sehingga ekspresi ARID1Akemungkinan dapat digunakan sebagai petanda adanya transformasi ganas padaendometriosis.ABSTRACT Background: Endometriosis is one of the most common gynecologicalabnormalities found. Endometriosis cyst in the ovary also exhibited changes inepithelial cyst just like endometrium in the uterus. Changes in the epithelial cellsalso include metaplasia, hyperplasia, atyphia even changes toward malignancharacteristics. Nowadays, there are some research that linked endometriosis andclear cell ovarian cancer which is known with endometriosis-associated ovariancarcinoma (EAOC) it is reported that there?s a mutation that activated tumorsuppressor gene (ARID1A), so protein BAF250a is not expressed in Clear CellCarcinoma (CCC) in the ovarium.Materials and Methods: Immunohistochemistry staining of ARID1A were donein 20 samples of non-atypical endometriosis, 20 samples of atypicalendometriosis, 20 samples of CCC in the ovarium from the year 2012 until march2015. From the group that experienced CCC we get 9 cases of EAOC. After that,we see if there?s any difference in the percentage of ARID1A expression in nonatypicalendometrosis, atypical endometriosis, CCC in the ovarium andendometriosis with CCC( EAOC).Results: In non-atypical endometriosis, atypical and CCC cases groups there aresignificant differences on the percentage of ARID1A expression (Kruskal-Walistest p=0,0035). Post Hoc analysis were done using Mann-Whitney test and thereare significant differences on ARID1A expression between non-atypical andatypical endometriosis with CCC (p=0,001 and p=0,0015). In non-atypicalendometriosis, atypical and EAOC groups there are significant differences on thepercentage of ARID1A expression (Kruskal-Walis test p=0,011). Post Hocanalysis were done using Mann-Whitney test and there are significant differenceson ARID1A expression between non-atypical and atypical endometriosis withEAOC (p=0,005 and p=0,008).Conclusion: Expression of ARID1A in non atypical and atypical endometriosisare significantly higher compared to ovarian CCC and EAOC. So, we can say thatARID1A may be used as a marker for malignancy transformation inendometriosis.;Background: Endometriosis is one of the most common gynecologicalabnormalities found. Endometriosis cyst in the ovary also exhibited changes inepithelial cyst just like endometrium in the uterus. Changes in the epithelial cellsalso include metaplasia, hyperplasia, atyphia even changes toward malignancharacteristics. Nowadays, there are some research that linked endometriosis andclear cell ovarian cancer which is known with endometriosis-associated ovariancarcinoma (EAOC) it is reported that there?s a mutation that activated tumorsuppressor gene (ARID1A), so protein BAF250a is not expressed in Clear CellCarcinoma (CCC) in the ovarium.Materials and Methods: Immunohistochemistry staining of ARID1A were donein 20 samples of non-atypical endometriosis, 20 samples of atypicalendometriosis, 20 samples of CCC in the ovarium from the year 2012 until march2015. From the group that experienced CCC we get 9 cases of EAOC. After that,we see if there?s any difference in the percentage of ARID1A expression in nonatypicalendometrosis, atypical endometriosis, CCC in the ovarium andendometriosis with CCC( EAOC).Results: In non-atypical endometriosis, atypical and CCC cases groups there aresignificant differences on the percentage of ARID1A expression (Kruskal-Walistest p=0,0035). Post Hoc analysis were done using Mann-Whitney test and thereare significant differences on ARID1A expression between non-atypical andatypical endometriosis with CCC (p=0,001 and p=0,0015). In non-atypicalendometriosis, atypical and EAOC groups there are significant differences on thepercentage of ARID1A expression (Kruskal-Walis test p=0,011). Post Hocanalysis were done using Mann-Whitney test and there are significant differenceson ARID1A expression between non-atypical and atypical endometriosis withEAOC (p=0,005 and p=0,008).Conclusion: Expression of ARID1A in non atypical and atypical endometriosisare significantly higher compared to ovarian CCC and EAOC. So, we can say thatARID1A may be used as a marker for malignancy transformation inendometriosis.;Background: Endometriosis is one of the most common gynecologicalabnormalities found. Endometriosis cyst in the ovary also exhibited changes inepithelial cyst just like endometrium in the uterus. Changes in the epithelial cellsalso include metaplasia, hyperplasia, atyphia even changes toward malignancharacteristics. Nowadays, there are some research that linked endometriosis andclear cell ovarian cancer which is known with endometriosis-associated ovariancarcinoma (EAOC) it is reported that there?s a mutation that activated tumorsuppressor gene (ARID1A), so protein BAF250a is not expressed in Clear CellCarcinoma (CCC) in the ovarium.Materials and Methods: Immunohistochemistry staining of ARID1A were donein 20 samples of non-atypical endometriosis, 20 samples of atypicalendometriosis, 20 samples of CCC in the ovarium from the year 2012 until march2015. From the group that experienced CCC we get 9 cases of EAOC. After that,we see if there?s any difference in the percentage of ARID1A expression in nonatypicalendometrosis, atypical endometriosis, CCC in the ovarium andendometriosis with CCC( EAOC).Results: In non-atypical endometriosis, atypical and CCC cases groups there aresignificant differences on the percentage of ARID1A expression (Kruskal-Walistest p=0,0035). Post Hoc analysis were done using Mann-Whitney test and thereare significant differences on ARID1A expression between non-atypical andatypical endometriosis with CCC (p=0,001 and p=0,0015). In non-atypicalendometriosis, atypical and EAOC groups there are significant differences on thepercentage of ARID1A expression (Kruskal-Walis test p=0,011). Post Hocanalysis were done using Mann-Whitney test and there are significant differenceson ARID1A expression between non-atypical and atypical endometriosis withEAOC (p=0,005 and p=0,008).Conclusion: Expression of ARID1A in non atypical and atypical endometriosisare significantly higher compared to ovarian CCC and EAOC. So, we can say thatARID1A may be used as a marker for malignancy transformation inendometriosis.;Background: Endometriosis is one of the most common gynecologicalabnormalities found. Endometriosis cyst in the ovary also exhibited changes inepithelial cyst just like endometrium in the uterus. Changes in the epithelial cellsalso include metaplasia, hyperplasia, atyphia even changes toward malignancharacteristics. Nowadays, there are some research that linked endometriosis andclear cell ovarian cancer which is known with endometriosis-associated ovariancarcinoma (EAOC) it is reported that there?s a mutation that activated tumorsuppressor gene (ARID1A), so protein BAF250a is not expressed in Clear CellCarcinoma (CCC) in the ovarium.Materials and Methods: Immunohistochemistry staining of ARID1A were donein 20 samples of non-atypical endometriosis, 20 samples of atypicalendometriosis, 20 samples of CCC in the ovarium from the year 2012 until march2015. From the group that experienced CCC we get 9 cases of EAOC. After that,we see if there?s any difference in the percentage of ARID1A expression in nonatypicalendometrosis, atypical endometriosis, CCC in the ovarium andendometriosis with CCC( EAOC).Results: In non-atypical endometriosis, atypical and CCC cases groups there aresignificant differences on the percentage of ARID1A expression (Kruskal-Walistest p=0,0035). Post Hoc analysis were done using Mann-Whitney test and thereare significant differences on ARID1A expression between non-atypical andatypical endometriosis with CCC (p=0,001 and p=0,0015). In non-atypicalendometriosis, atypical and EAOC groups there are significant differences on thepercentage of ARID1A expression (Kruskal-Walis test p=0,011). Post Hocanalysis were done using Mann-Whitney test and there are significant differenceson ARID1A expression between non-atypical and atypical endometriosis withEAOC (p=0,005 and p=0,008).Conclusion: Expression of ARID1A in non atypical and atypical endometriosisare significantly higher compared to ovarian CCC and EAOC. So, we can say thatARID1A may be used as a marker for malignancy transformation inendometriosis."

"Latar Belakang: Tipe terbanyak dari limfoma non-Hodgkin (LNH) sel B adalah Diffuse Large B-cell Lymphoma (DLBCL) yang merupakan entitas heterogen. Hans membagi DLBCL menjadi subtipe Germinal Center B-cell like (GCB) dan non-Germinal Center B-cell like (non-GCB) dengan tehnik pemeriksaan imunohistokimia menggunakan CD10, BCL6 dan MUM1. Caspase-3 adalah protein yang berperan utama dalam mekanisme apoptosis dan dapat mengalami mutasi somatik pada LNH. Imunoekspresi Caspase-3 dapat berhubungan dengan kesintasan pasien DLBCL. Tujuan penelitian ini untuk mengetahui perbedaan imunoekspresi Caspase-3 pada DLBCL subtipe GCB dan non-GCB. Bahan dan Metode: Pemeriksaaan imunoekspresi Caspase-3 pada 41 kasus DLBCL yang terdiri atas 18 kasus subtipe GCB dan 23 kasus non-GCB dilakukan dengan tehnik imunohistokimia . Subjek penelitian berasal dari RSCM dan enam rumah sakit lainnya di Jakarta. Hasil: Kasus DLBCL subtipe GCB memberikan hasil positif pada pulasan Caspase-3 berjumlah 13 kasus (72%) dan negatif berjumlah 5 kasus (28%). Hasil yang didapatkan dari subtipe non-GCB yaitu positif berjumlah 5 kasus (22%) dan negatif berjumlah 18 kasus (78%). Terdapat perbedaan bermakna imunoekspresi Caspase-3 antara DLBCL subtipe GCB dengan subtipe non-GCB (p = 0,002). Kesimpulan: Imunoekspresi Caspase-3 menunjukkan positivitas yang lebih tinggi pada DLBCL subtipe GCB dibandingkan dengan subtipe non-GCB.

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Background: The most common type of B-cell non-Hodgkin lymphoma (NHL) is diffuse large B-cell lymphoma (DLBCL) which is heterogeneous. Hans divided DLBCL into Germinal Center B-cell-like (GCB) and non-Germinal Center B-cell-like (non-GCB) subtypes by using immunohistochemistry staining with CD10, BCL6 and MUM1. Caspase-3 is a protein that functions as a key role in the mechanism of apoptosis and may have somatic mutation in NHL. Immunoexpression of Caspase-3 could be associated with the survival of DLBCL patient. This study was performed to analyze the difference of Caspase-3 immunoexpression between GCB and non-GCB subtypes of DLBCL.

Materials and Methods: Caspase-3 immunoexpression examination was performed in 41 DLBCL cases, consisted of 18 cases of GCB and 23 cases of non-GCB subtypes by using immunohistochemistry technique. The cases came from RSCM and 6 other hospitals in Jakarta.

Result: The number of GCB subtype which showed positive Caspase-3 staining was 13 cases (72%) and negative in 5 cases (28%). The number of non-GCB subtype which showed positive Caspase-3 staining was 5 cases (22%) and negative in 18 cases (78%). There was significant difference of Caspase-3 immunoexpression between GCB and non-GCB subtype of DLBCL (p = 0,002).

Conclusion: The positivity of Caspase-3 expression was higher in GCB subtype than non-GCB subtype."