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Hasil Pencarian

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Aulia Sekar Putri
Abstrak :
Pertanian merupakan salah satu sektor utama yang berperan dalam perkembangan ekonomi Indonesia. Produktivitas pertanian dan ketahanan pangan berkaitan erat dengan kondisi kesehatan tanaman. Salah satu patogen penginfeksi tanaman adalah fungi. Pengendali hayati atau biokontrol merupakan teknik pengendalian menggunakan aktivitas metabolisme agen hayati seperti bakteri untuk mengendalikan patogen tanaman. Penelitian terdahulu berhasil mengisolasi bakteri saluran pencernaan black soldier fly larvae (BSFL) dan diketahui mampu mendukung pertumbuhan tanaman. Penelitian ini bertujuan untuk mengetahui kemampuan 10 isolat bakteri saluran pencernaan BSFL terhadap 4 fungi patogen tanaman, yaitu Ganoderma boninense, Fusarium oxysporum, Colletotrichum siamense KA, dan Curvularia lunata BM. Pengujian aktivitas antifungi isolat bakteri dilakukan melalui uji aktivitas antagonistik dan uji aktivitas antibiosis yang dilanjutkan dengan uji aktivitas enzim ekstraseluler isolat potensial. Uji aktivitas antagonistik menggunakan metode dual culture secara kualitatif dan semi-kuantitatif. Uji aktivitas antibiosis menggunakan filtrat medium fermentasi berusia 5 hari yang dicampur dengan bubuk medium potato dextrose agar. Uji aktivitas enzim ekstraseluler isolat potensial meliputi uji enzim protease, lipase, amilase, kitinase, dan selulase menggunakan medium selektif. Hasil uji aktivitas antagonistik menunjukkan 4 dari 10 isolat bakteri saluran pencernaan BSFL, yaitu G7, G17, G20, G21 mampu menghambat pertumbuhan keempat fungi uji. Hasil uji aktivitas antibiosis menunjukkan bahwa efektivitas terbesar diperoleh dari filtrat medium fermentasi isolat G17 dalam menghambat pertumbuhan fungi Ganoderma boninense dengan persentase hambatan sebesar 85,56% – 91,98%. Hasil uji aktivitas enzim menunjukkan isolat G17 memiliki aktivitas enzim ektraseluler protease, lipase, dan kitinase. Isolat G17 teridentifikasi sebagai Stenotrophomonas maltophilia. ......Agriculture is one of the main sectors that play a role in Indonesia's economic development. Agricultural productivity and food security are closely related to plant health conditions. One of the plant infecting pathogens is a fungus. Biological control or biocontrol is a control technique using the metabolic activity of biological agents such as bacteria to control plant pathogens. Previous studies have succeeded in isolating black soldier fly larvae (BSFL) gut bacteria and are known to be able to support plant growth. This study aims to determine the ability of 10 bacterial isolates from BSFL gut against 4 plant pathogenic fungi, namely Ganoderma boninense, Fusarium oxysporum, Colletotrichum siamense KA, and Curvularia lunata BM. Antifungal activity assay of bacterial isolates was carried out through antagonistic activity assay and antibiosis activity assay followed by potential isolates extracellular enzyme activity assay. The antagonistic activity assay used the dual culture method qualitatively and semi-quantitatively. The antibiosis activity assay used 5 days old fermented medium filtrate mixed with potato dextrose agar medium powder. The extracellular enzyme activity assay of potential isolates included protease, lipase, amylase, chitinase, and cellulase enzyme assay using selective media. The results of the antagonistic activity assay showed that 4 out of 10 BSFL digestive tract bacterial isolates namely G7, G17, G20, G21 were able to inhibit the growth of the four tested fungi. The results of the antibiosis activity assay showed that the greatest effectiveness was obtained from the fermented medium filtrate of isolate G17 in inhibiting the growth of the Ganoderma boninense fungus with an inhibition percentage of 85.56% – 91.98%. The results of the enzyme activity assay showed that isolate G17 had extracellular enzyme activity of protease, lipase, and chitinase. Isolate G17 was identified as Stenotrophomonas maltophilia.
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023
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UI - Skripsi Membership  Universitas Indonesia Library
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Bella Rineka Putri
Abstrak :
ABSTRAK
Penelitian tentang potensi antagonis bakteri Gram-positif Bacillus siamensis LDR terhadap pertumbuhan 2 isolat Asp. niger ABP dan ART memiliki selesai. Uji antagonis dilakukan dengan menggunakan metode kultur ganda yang berbeda, yaitu: kultur cakram ganda dan kultur piring tuang. Hasil pengujian pada metode kultur cakram ganda menunjukkan bahwa B. siamensis LDR mampu menghambat pertumbuhan diameter Asp. niger ART (44,83%) dan ABP (39,95%). Sedangkan pada metode pelat tuang, kultur, B. siamensis LDR mampu menghambat pertumbuhan Asp. niger ART (87,98%) dan ABP (92,43%). Fermentasi senyawa antijamur dari B. siamensis telah dilakukan dengan metode fermentasi menggunakan media Potato Dextrose Broth untuk inkubasi 10, 12 dan 14 hari. Filtrat hasil fermentasi digunakan sebagai pelarut untuk medium medium PDA dan PDB dalam uji antibiotik. Uji antibiotik menunjukkan bahwa efektivitas terbesar diperoleh dari filtrat fermentasi 14 hari. Hasil pengujian pada media PDA berdasarkan diameter pertumbuhan koloni menunjukkan terhambatnya pertumbuhan Asp. niger ART (74,53%) dan ABP (62,13%). Sedangkan penghambatan pertumbuhan pada medium PDB yang ditentukan berdasarkan produksi biomassa menunjukkan penurunan sebesar 79,80% pada Asp. niger ART dan 51,22% pada Asp. niger ABP versus kontrol. Uji antibiosis dengan metode difusi cakram pada waktu inkubasi media fermentasi hari ke 14 juga selesai. Hasil pengujian pada metode difusi cakram menunjukkan bahwa penghambatan pertumbuhan Asp. niger ART dan ABP pada konsentrasi 2.500 ppm hingga 20.000 ppm.
ABSTRACT
Research on the potential antagonist of Gram-positive bacteria Bacillus siamensis LDR to the growth of 2 isolates of Asp. niger ABP and ART have finished. The antagonist test was carried out using different multiple culture methods, namely: double disc culture and pour plate culture. The test results on the double disc culture method showed that B. siamensis LDR was able to inhibit the growth of Asp diameter. niger ART (44.83%) and ABP (39.95%). While in the pour plate method, culture, B. siamensis LDR was able to inhibit the growth of Asp. niger ART (87.98%) and ABP (92.43%). Fermentation of antifungal compounds from B. siamensis has been carried out by fermentation method using Potato Dextrose Broth media for incubation 10, 12 and 14 days. The fermented filtrate was used as a solvent for PDA and PDB medium in the antibiotic test. Antibiotic tests show that the greatest effectiveness obtained from 14 days fermentation filtrate. The test results on PDA media based on the diameter of the colony growth showed inhibition of Asp growth. niger ART (74.53%) and ABP (62.13%). Meanwhile, the inhibition of growth in the GDP medium which was determined based on biomass production showed a decrease of 79.80% in Asp. niger ART and 51.22% on Asp. niger ABP versus control. Antibiosis test using the disc diffusion method at the incubation time of the 14th day of fermentation media was also completed. The test results on the disc diffusion method showed that the inhibition of the growth of Asp. niger ART and ABP at a concentration of 2,500 ppm up to 20,000 ppm.
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2019
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UI - Skripsi Membership  Universitas Indonesia Library
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Inneke Kusanggraeni
Abstrak :
Berbagai masalah muncul dalam makanan dan produk pertanian yang tercemar dengan cetakan Aspergillus niger. Pertumbuhan A. niger dapat dikelola dengan metode biokontrol menggunakan senyawa antijamur yang dihasilkan melalui proses fermentasi. Komposisi nutrisi merupakan faktor penting dalam proses fermentasi. Penelitian telah dilakukan terhadap produksi senyawa antijamur dari Bacillus siamensis LDR melawan dua galur A. niger ABP dan ART. Modifikasi media Czapek-Dox dengan Sumber karbon pati beras, sukrosa dan ekstrak ragi digunakan sebagai sumber karbon media fermentasi. Fermentasi dilakukan selama 10, 12, dan 14 hari dengan metode diam budaya. Uji antibiotik dilakukan dengan menggunakan filtrat fermentasi sebagai pelarut internal PDB dan PDA sedang. Hasil pengujian diukur berdasarkan biomassa pada medium cair PDB, dan diameter pertumbuhan dalam media padat PDA dari A. niger ABP dan ART. Uji Filtrat pertumbuhan antibiosis B. siamensis LDR terhadap A. niger menunjukkan ABP efektivitas tertinggi pada hari ke-10 fermentasi dengan penurunan biomassa hingga 99,20% dan penghambatan pertumbuhan diameter mencapai 83,25%. Efektivitas tertinggi terhadap A. niger ART pada hari ke-12 fermentasi dengan penurunan biomassa hingga 89,62% dan diameter penghambatan pertumbuhan mencapai 87,95%. Tes antibiotik juga dilakukan dengan metode difusi ekstrak kasar senyawa antijamur dari media fermentasi pada hari ke 12. Media diekstraksi dengan metode ekstraksi cair-cair menggunakan etil asetat. Uji antibiotik dengan ekstrak kasar dilakukan pada konsentrasi 2.500, 5.000, 10.000, dan 20.000 ppm. Secara kualitatif, ekstrak kasar bersifat antijamur menunjukkan penghambatan pertumbuhan terhadap A. niger ABP dan HAART pada konsentrasi 2.500 ppm. ......Problems arose in food and agricultural products tainted with Aspergillus niger molds. A. niger growth can be managed by biocontrol methods using antifungal compounds produced through the fermentation process. The nutritional composition is an important factor in the fermentation process. Research has been conducted on the production of an antifungal compound from Bacillus siamensis LDR against two A. niger ABP strains and ART. Modification of Czapek-Dox media with carbon sources of rice starch, sucrose and yeast extract were used as carbon sources in the fermentation medium. Fermentation was carried out for 10, 12, and 14 days with culture still method. Antibiotic test was carried out using fermentation filtrate as internal solvent of medium PDB and PDA. The test results were measured based on biomass in PDB liquid medium, and growth diameter in PDA solid media from A. niger ABP and ART. Test The antibiosis growth filtrate of B. siamensis LDR against A. niger showed the highest ABP effectiveness on the 10th day of fermentation with a decrease in biomass up to 99.20% and inhibition of diameter growth reaching 83.25%. Highest effectiveness against A. niger ART on the 12th day of fermentation with a decrease in biomass up to 89.62% and the diameter of growth inhibition reaching 87.95%. Antibiotic tests were also carried out by diffusion method of crude extract of antifungal compounds from the fermentation medium on day 12. The media was extracted by the liquid-liquid extraction method using ethyl acetate. Antibiotic assays with crude extracts were carried out at concentrations of 2,500, 5,000, 10,000, and 20,000 ppm. Qualitatively, the crude extract was antifungal and showed growth inhibition against A. niger ABP and HAART at a concentration of 2,500 ppm.
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2019
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UI - Skripsi Membership  Universitas Indonesia Library