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"Polisiklik Aromatik Hidrokarbon (PAH) merupakan salah satu jenis bahan pencemar organik yang dapat dihasilkan dari pembakaran yang tak sempurna (pirogenik) ataupun dari kegiatan perminyakan (petrogenik). Pertambakan adalah salah satu kawasan yang rentan akan bahan pencemar organik. Pada penelitian ini dilakukan analisis terhadap sedimen dan Udang Windu (Penaeus monodon) di pertambakan untuk mengetahui kadar senyawa PAH yang dipengaruhi oleh tataguna lahan dan vegetasi mangrove. Sampel yang sudah kering kemudian diekstraksi dengan soxhlet selama ± 18 jam dengan 250 mL pelarut campuran (1:1) n-heksan : diklorometan (DCM) lalu di fraksinasi menggunakan kolom berisi silika gel, alumina dan natrium sulfat dengan 40 mL pelarut campuran (1:1) n-heksan : diklorometan (DCM) untuk mendapatkan fraksi aromatik. Hasil fraksinasi kemudian dievaporasi dan diblow up dengan gas helium hingga tepat 1 mL, lalu diambil 2 μL untuk analisa dengan GC-MS (Gas Chromatography-Mass Spectrometry). Hasil analisa menunjukkan bahwa konsentrasi Naphthalene di sedimen Blanakan dan Marunda berkisar antara 0.0944 ng.g-1 - 9.9069 ng.g-1 dan 0.1691 ng.g-1 - 8.3503 ng.g-1. Sedangkan untuk konsentrasi benzo(a)pyrene di sedimen blanakan dan Marunda berkisar antara 2.6294 ng.g-1 - 5.2302 ng.g-1 dan 4.0760 ng.g-1 - 6.3368 ng.g-1. Konsentrasi senyawa naphthalene pada tubuh udang windu di kawasan Marunda dan Blanakan sebesar 4.7080 ng.g-1 dan 1.6322 ng.g-1 serta untuk senyawa benzo(a)pyrene di kawasan Marunda dan Blanakan sebesar 1.5367 ng.g-1 dan 1.2910 ng.g-1. ......Polycyclic Aromatic Hydrocarbons (PAH) is one type of organic pollutants can be produced from incomplete combustion (pyrogenic) or from petroleum activities (petrogenik). Aquaculture is one of the areas that are vulnerable to organic pollutants. In this research, analysis of sediment and tiger prawn (Penaeus monodon) in aquaculture to determine levels of PAH compounds that are affected by land use and mangrove vegetation. The dried samples were then extracted by Soxhlet for ± 18 hours with 250 mL solvent mixture (1:1) n-hexane: dichloromethane (DCM) and fractionated using a column containing silica gel, alumina and sodium sulfate with 40 mL of solvent mixture (1 : 1) n-hexane: dichloromethane (DCM) to obtain the aromatic fraction. Results of fractionation then evaporated and blow up with helium gas to exactly 1 mL, 2 μL of result then taken for analysis by GC-MS (Gas Chromatography-Mass Spectrometry). The analysis shows that the concentration of Naphthalene in sediments Blanakan and Marunda ranged between 0.0944 ng.g-1 - 9.9069 ng.g-1 and 0.1691 ng.g-1 - 8.3503 ng.g-1. As for the concentration of benzo(a)pyrene in sediment Blanakan and Marunda range between 2.6294 ng.g-1 - 5.2302 ng.g-1 and 4.0760 ng.g-1 - 6.3368 ng.g-1. The concentration of naphthalene compound in prawn's body in the Marunda and Blanakan ranged from 4.7080 ng.g-1 to 1.6322 ng.g-1 and for benzo(a)pyrene compound in the Marunda and Blanakan from 1.5367 ng.g-1 to 1.2910 ng.g-1."

"Kebakaran hutan dan lahan menghasilkan asap yang diketahui mengandung Polycyclic Aromatic Hydrocarbon (PAH). Efek adanya kerusakan oksidatif pada DNA akibat paparan asap kebakaran hutan dan lahan terhadap risiko kanker diselidiki melalui deteksi biomarker 8-Hidroksi-2’-Deoksiguanosin (8-OHdG) dan 1-Hidroksipiren (1- OHP), metabolit utama piren, sebagai indikator paparan PAH dalam urin. Analisis biomarker paparan PAH dalam urin 24 jam dilakukan secara acak dalam suatu populasi di Kota Dumai, Provinsi Riau. Kandungan 8-OHdG dalam sampel urin dianalisis menggunakan HPLC detektor UV dengan fasa gerak buffer natrium fosfat 0,1 M pH 6,7 dan metanol (85:15, v/v). Sementara, kandungan 1-OHP dalam urin dideteksi menggunakan HPLC detektor flourosens dengan eluen metanol dan air (60:40, v/v). Analisis kedua senyawa tersebut dilakukan dengan kromatografi fasa terbalik mode isokratik. Hasil pengujian menunjukan bahwa 8-OHdG terdeteksi pada seluruh sampel dalam rentang konsentrasi 25,29 g/L hingga 2,16 mg/L urin (n = 11), lebih tinggi dibandingkan dengan konsentrasi 8-OHdG dalam urin individu sehat (1,42 μg/L – 4,25 μg/L). 1-OHP dalam urin juga terdeteksi dalam empat dari lima sampel yang diuji, menandakan bahwa terdapat potensi besar kerusakan oksidatif DNA akibat paparan PAH......orest fires generate woodsmoke that contain Polycyclic Aromatic Hydrocarbon (PAH). The effect of DNA oxidative damage due to woodsmoke exposure on cancer risk can be investigated through the detection of urinary 8-Hydroxy-2’-Deoxyguanosin (8-OHdG) and 1-Hydroxypyrene, main metabolite of pyrene, as indicators of PAH exposure. Analysis biomarkers of PAH exposure in 24 hours urine was performed within a population randomized in Dumai City, Riau Province. The 8-OHdG levels in urine samples were analyzed by using HPLC with UV detector using sodium phosphate buffer 0.1 M pH 6.7 and methanol (85:15, v/v) as mobile phase. Meanwhile, 1-OHP levels in urine was detected by using a HPLC with fluorosens detector using methanol and water (60:40, v/v) eluent. Analysis of both compounds was performed by reverse phase chromatography with isoratic mode. The results showed that 8-OHdG was detected in all samples with concentration range of 25.29 μg/L to 2.16 mg/L urine (n = 11), higher than urinary 8-OHdG of health person (1,42 μg/L – 4,25 μg/L). Urinary 1- OHP was also detected in four of five samples. It indicates that there was a high potential of DNA damage caused by PAH exposure"

"[This book provides a thorough and up-to-date overview of the aryl hydrocarbon receptor (AHR) and its unique dual role in toxicology and biology. The coverage includes epigenetic mechanisms, gene expression, reproductive and developmental toxicity, signal transduction, and transgenic animal models. Featuring an internationally recognized team of authors at the forefront of AHR research, this resource provides a comprehensive reference for readers interested in understanding the full spectrum of AHR, from basic concepts, toxicology analysis, and models to polymorphism and related diseases., This book provides a thorough and up-to-date overview of the aryl hydrocarbon receptor (AHR) and its unique dual role in toxicology and biology. The coverage includes epigenetic mechanisms, gene expression, reproductive and developmental toxicity, signal transduction, and transgenic animal models. Featuring an internationally recognized team of authors at the forefront of AHR research, this resource provides a comprehensive reference for readers interested in understanding the full spectrum of AHR, from basic concepts, toxicology analysis, and models to polymorphism and related diseases.]"